Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00590
(648 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g18020.1 68415.m02094 60S ribosomal protein L8 (RPL8A) 238 3e-63
At4g36130.1 68417.m05142 60S ribosomal protein L8 (RPL8C) riboso... 233 7e-62
At3g51190.1 68416.m05604 60S ribosomal protein L8 (RPL8B) riboso... 231 3e-61
At2g44065.2 68415.m05480 ribosomal protein L2 family protein sim... 49 3e-06
At2g44065.1 68415.m05479 ribosomal protein L2 family protein sim... 49 3e-06
At4g14250.1 68417.m02198 UBX domain-containing protein low simil... 47 1e-05
At4g24390.2 68417.m03498 F-box family protein (FBX14) similar to... 31 0.87
At4g24390.1 68417.m03497 F-box family protein (FBX14) similar to... 31 0.87
At2g22730.1 68415.m02694 transporter-related low similarity to s... 30 1.2
At3g61210.1 68416.m06850 embryo-abundant protein-related similar... 29 2.0
At2g38630.1 68415.m04745 expressed protein 29 3.5
At3g51740.1 68416.m05673 leucine-rich repeat transmembrane prote... 28 6.1
At1g11080.1 68414.m01269 serine carboxypeptidase S10 family prot... 28 6.1
At1g10730.1 68414.m01223 clathrin adaptor complexes medium subun... 28 6.1
At4g33410.1 68417.m04748 signal peptide peptidase family protein... 27 8.1
At3g16950.1 68416.m02166 dihydrolipoamide dehydrogenase 1, plast... 27 8.1
At1g68530.2 68414.m07829 very-long-chain fatty acid condensing e... 27 8.1
At1g68530.1 68414.m07828 very-long-chain fatty acid condensing e... 27 8.1
>At2g18020.1 68415.m02094 60S ribosomal protein L8 (RPL8A)
Length = 258
Score = 238 bits (582), Expect = 3e-63
Identities = 109/168 (64%), Positives = 136/168 (80%), Gaps = 1/168 (0%)
Frame = +3
Query: 9 VRAQRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAVVHFR 188
+RAQRKGAGSVF SHT RKG K RSLD+ ER+GY+KGVV +IIHDPGRGAPLA V FR
Sbjct: 5 IRAQRKGAGSVFKSHTHHRKGPAKFRSLDFGERNGYLKGVVTEIIHDPGRGAPLARVTFR 64
Query: 189 DPYKFKTRKELFIAPEALHR-PIVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKMGDRG 365
P++FK +KELF+A E ++ +YCGKKATL VGNV+P+ ++PEG +VCN+E +GDRG
Sbjct: 65 HPFRFKKQKELFVAAEGMYTGQFLYCGKKATLVVGNVLPLRSIPEGAVVCNVEHHVGDRG 124
Query: 366 RLARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRGMVGIVAGG 509
LARASG++A VI HNPD+ TR+KLPSG+KK++PS R M+G VAGG
Sbjct: 125 VLARASGDYAIVIAHNPDSDTTRIKLPSGSKKIVPSGCRAMIGQVAGG 172
Score = 94.3 bits (224), Expect = 6e-20
Identities = 38/46 (82%), Positives = 41/46 (89%)
Frame = +2
Query: 509 GRIDKPILKAGRAYHKYKVKRNCWPYVRGVAMNPVEHPHGGGTHQH 646
GR +KP+LKAG AYHKY+VKRN WP VRGVAMNPVEHPHGGG HQH
Sbjct: 173 GRTEKPMLKAGNAYHKYRVKRNSWPKVRGVAMNPVEHPHGGGNHQH 218
>At4g36130.1 68417.m05142 60S ribosomal protein L8 (RPL8C) ribosomal
protein L8, cytosolic, tomato, PIR1:R5TOL8
Length = 258
Score = 233 bits (570), Expect = 7e-62
Identities = 106/168 (63%), Positives = 134/168 (79%), Gaps = 1/168 (0%)
Frame = +3
Query: 9 VRAQRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAVVHFR 188
+RAQRKGAGSVF SHT RKG K RSLD+ ER+GY+KGVV +IIHDPGRGAPLA V FR
Sbjct: 5 IRAQRKGAGSVFKSHTHHRKGPAKFRSLDFGERNGYLKGVVTEIIHDPGRGAPLARVAFR 64
Query: 189 DPYKFKTRKELFIAPEALHR-PIVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKMGDRG 365
P++FK +KELF+A E ++ +YCGKKATL VGNV+P+ ++PEG ++CN+E +GDRG
Sbjct: 65 HPFRFKKQKELFVAAEGMYTGQFLYCGKKATLVVGNVLPLRSIPEGAVICNVEHHVGDRG 124
Query: 366 RLARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRGMVGIVAGG 509
ARASG++A VI HNPD +R+KLPSG+KK++PS R M+G VAGG
Sbjct: 125 VFARASGDYAIVIAHNPDNDTSRIKLPSGSKKIVPSGCRAMIGQVAGG 172
Score = 98.3 bits (234), Expect = 4e-21
Identities = 39/46 (84%), Positives = 42/46 (91%)
Frame = +2
Query: 509 GRIDKPILKAGRAYHKYKVKRNCWPYVRGVAMNPVEHPHGGGTHQH 646
GR +KP+LKAG AYHKY+VKRNCWP VRGVAMNPVEHPHGGG HQH
Sbjct: 173 GRTEKPMLKAGNAYHKYRVKRNCWPKVRGVAMNPVEHPHGGGNHQH 218
>At3g51190.1 68416.m05604 60S ribosomal protein L8 (RPL8B) ribosomal
protein L8, cytosolic - Arabidopsis thaliana, PIR:T04582
Length = 260
Score = 231 bits (565), Expect = 3e-61
Identities = 107/169 (63%), Positives = 136/169 (80%), Gaps = 2/169 (1%)
Frame = +3
Query: 9 VRAQRKGA-GSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAVVHF 185
+RAQRKGA GSVF SHT RKG K RSLDY ER+GY+KG+V +IIHDPGRGAPLA V F
Sbjct: 5 IRAQRKGAAGSVFKSHTHHRKGPAKFRSLDYGERNGYLKGLVTEIIHDPGRGAPLARVAF 64
Query: 186 RDPYKFKTRKELFIAPEALHR-PIVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKMGDR 362
R P+++ +KELF+A E ++ +YCGKKA L VGNV+P+G++PEG ++CN+E +GDR
Sbjct: 65 RHPFRYMKQKELFVAAEGMYTGQYLYCGKKANLMVGNVLPLGSIPEGAVICNVELHVGDR 124
Query: 363 GRLARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRGMVGIVAGG 509
G LARASG++A VI HNP++ TRVKLPSG+KK+LPS+ R M+G VAGG
Sbjct: 125 GALARASGDYAIVIAHNPESNTTRVKLPSGSKKILPSACRAMIGQVAGG 173
Score = 97.9 bits (233), Expect = 5e-21
Identities = 39/46 (84%), Positives = 40/46 (86%)
Frame = +2
Query: 509 GRIDKPILKAGRAYHKYKVKRNCWPYVRGVAMNPVEHPHGGGTHQH 646
GR +KP LKAG AYHKYK KRNCWP VRGVAMNPVEHPHGGG HQH
Sbjct: 174 GRTEKPFLKAGNAYHKYKAKRNCWPVVRGVAMNPVEHPHGGGNHQH 219
>At2g44065.2 68415.m05480 ribosomal protein L2 family protein
similar to ribosomal protein L2 [Gossypium arboreum]
GI:17644114; contains Pfam profile PF03947: Ribosomal
Proteins L2, C-terminal domain
Length = 214
Score = 48.8 bits (111), Expect = 3e-06
Identities = 25/73 (34%), Positives = 41/73 (56%)
Frame = +3
Query: 285 VGNVMPVGAMPEGTIVCNLEEKMGDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKV 464
+G+ MP+G M GTI+ N+E G ++ RA+G A ++ P + +KLPSG K
Sbjct: 59 IGSSMPLGMMRIGTIIHNIEMNPGQGAKMVRAAGTNAKIL-KEPAKGKCLIKLPSGDTKW 117
Query: 465 LPSSNRGMVGIVA 503
+ + R +G V+
Sbjct: 118 INAKCRATIGTVS 130
Score = 40.7 bits (91), Expect = 8e-04
Identities = 18/30 (60%), Positives = 21/30 (70%), Gaps = 4/30 (13%)
Frame = +2
Query: 557 YKVKRNCW----PYVRGVAMNPVEHPHGGG 634
YK ++ W P VRGVAMNP +HPHGGG
Sbjct: 140 YKAGQSRWLGIRPKVRGVAMNPCDHPHGGG 169
>At2g44065.1 68415.m05479 ribosomal protein L2 family protein
similar to ribosomal protein L2 [Gossypium arboreum]
GI:17644114; contains Pfam profile PF03947: Ribosomal
Proteins L2, C-terminal domain
Length = 214
Score = 48.8 bits (111), Expect = 3e-06
Identities = 25/73 (34%), Positives = 41/73 (56%)
Frame = +3
Query: 285 VGNVMPVGAMPEGTIVCNLEEKMGDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKV 464
+G+ MP+G M GTI+ N+E G ++ RA+G A ++ P + +KLPSG K
Sbjct: 59 IGSSMPLGMMRIGTIIHNIEMNPGQGAKMVRAAGTNAKIL-KEPAKGKCLIKLPSGDTKW 117
Query: 465 LPSSNRGMVGIVA 503
+ + R +G V+
Sbjct: 118 INAKCRATIGTVS 130
Score = 40.7 bits (91), Expect = 8e-04
Identities = 18/30 (60%), Positives = 21/30 (70%), Gaps = 4/30 (13%)
Frame = +2
Query: 557 YKVKRNCW----PYVRGVAMNPVEHPHGGG 634
YK ++ W P VRGVAMNP +HPHGGG
Sbjct: 140 YKAGQSRWLGIRPKVRGVAMNPCDHPHGGG 169
>At4g14250.1 68417.m02198 UBX domain-containing protein low
similarity to 60S ribosomal protein L2 [Nicotiana
tabacum] GI:9230281; contains Pfam profile PF00789: UBX
domain
Length = 724
Score = 46.8 bits (106), Expect = 1e-05
Identities = 21/29 (72%), Positives = 22/29 (75%), Gaps = 1/29 (3%)
Frame = +2
Query: 554 KYKVKRNCWPYVRGVAM-NPVEHPHGGGT 637
K +KRN W VRGVAM NPVEHPHGG T
Sbjct: 411 KLMIKRNMWAKVRGVAMMNPVEHPHGGRT 439
>At4g24390.2 68417.m03498 F-box family protein (FBX14) similar to
transport inhibitor response 1 protein GI:8777429 from
[Arabidopsis thaliana]
Length = 623
Score = 30.7 bits (66), Expect = 0.87
Identities = 19/76 (25%), Positives = 35/76 (46%)
Frame = +3
Query: 96 YAERHGYIKGVVKDIIHDPGRGAPLAVVHFRDPYKFKTRKELFIAPEALHRPIVYCGKKA 275
+ E +++ + D + P L + R P+ K R F++ E LHR +V +
Sbjct: 224 FPEGETHLESLSFDCVESPINFKALEELVVRSPFLKKLRTNRFVSLEELHRLMVRAPQLT 283
Query: 276 TLEVGNVMPVGAMPEG 323
+L G+ P +P+G
Sbjct: 284 SLGTGSFSP-DNVPQG 298
>At4g24390.1 68417.m03497 F-box family protein (FBX14) similar to
transport inhibitor response 1 protein GI:8777429 from
[Arabidopsis thaliana]
Length = 623
Score = 30.7 bits (66), Expect = 0.87
Identities = 19/76 (25%), Positives = 35/76 (46%)
Frame = +3
Query: 96 YAERHGYIKGVVKDIIHDPGRGAPLAVVHFRDPYKFKTRKELFIAPEALHRPIVYCGKKA 275
+ E +++ + D + P L + R P+ K R F++ E LHR +V +
Sbjct: 224 FPEGETHLESLSFDCVESPINFKALEELVVRSPFLKKLRTNRFVSLEELHRLMVRAPQLT 283
Query: 276 TLEVGNVMPVGAMPEG 323
+L G+ P +P+G
Sbjct: 284 SLGTGSFSP-DNVPQG 298
>At2g22730.1 68415.m02694 transporter-related low similarity to
spinster membrane proteins from [Drosophila
melanogaster] GI:12003974, GI:12003976, GI:12003972,
GI:12003970; contains Pfam profile PF00083: major
facilitator superfamily protein
Length = 510
Score = 30.3 bits (65), Expect = 1.2
Identities = 28/103 (27%), Positives = 39/103 (37%), Gaps = 2/103 (1%)
Frame = -3
Query: 568 FDLVLVVCP--SSFQNRFVNTSPPATIPTMPLLLDGRTFLAPDGSFTLVRLASGLCPITV 395
F V ++C + F+ ATIP LL G TFL FT L S I +
Sbjct: 337 FGAVTIICGIVGTLSGGFILDRVTATIPNAFKLLSGATFLGAVFCFTAFTLKSLYGFIAL 396
Query: 394 AKFPEARARRPLSPIFSSRLHTMVPSGIAPTGITFPTSRVAFF 266
E +P+ LH + PS + P + T + F
Sbjct: 397 FALGELLVFATQAPVNYVCLHCVKPS-LRPLSMAISTVAIHIF 438
>At3g61210.1 68416.m06850 embryo-abundant protein-related similar to
embryo-abundant protein [Picea glauca] GI:1350531
Length = 261
Score = 29.5 bits (63), Expect = 2.0
Identities = 17/53 (32%), Positives = 25/53 (47%)
Frame = -3
Query: 625 VRMLYRVHGNTTYIWPAVTFDLVLVVCPSSFQNRFVNTSPPATIPTMPLLLDG 467
V+ + R G +W V DL++ S R V+++ P PTM L DG
Sbjct: 125 VKRVLRKQGGIIVVW--VYNDLIITPKVDSIMKRLVDSTLPYRNPTMNLAFDG 175
>At2g38630.1 68415.m04745 expressed protein
Length = 467
Score = 28.7 bits (61), Expect = 3.5
Identities = 19/60 (31%), Positives = 33/60 (55%), Gaps = 2/60 (3%)
Frame = -2
Query: 290 SNFKSCFLSTINNWPV*S--FGSNEELLPCLELVWIAEVYNSQRCTSTRVMDYILNNSLD 117
+N K CFL+ + + S + N + L + V+ ++ Y+S +C STR+ +YIL D
Sbjct: 117 TNKKVCFLNVSPDEVIRSLFYNKNNDSLITVS-VYASDNYSSLKCRSTRI-EYILRGQAD 174
>At3g51740.1 68416.m05673 leucine-rich repeat transmembrane protein
kinase, putative brassinosteroid-insensitive protein
BRI1 - Arabidopsis thaliana, PIR:T09356
Length = 836
Score = 27.9 bits (59), Expect = 6.1
Identities = 17/51 (33%), Positives = 23/51 (45%)
Frame = +3
Query: 174 VVHFRDPYKFKTRKELFIAPEALHRPIVYCGKKATLEVGNVMPVGAMPEGT 326
+VHF P+ F L E + + KATLE GN + V + E T
Sbjct: 521 LVHFDGPFVFTADDLLCATAEIMGKSTYGTAYKATLEDGNEVAVKRLREKT 571
>At1g11080.1 68414.m01269 serine carboxypeptidase S10 family protein
similar to Serine carboxypeptidase II chains A and B
(SP:P08819) (EC 3.4.16.6) [Triticum aestivum (Wheat)]
Length = 492
Score = 27.9 bits (59), Expect = 6.1
Identities = 13/38 (34%), Positives = 20/38 (52%)
Frame = -2
Query: 314 HSSNRHHISNFKSCFLSTINNWPV*SFGSNEELLPCLE 201
H+S+ ++ N+ C + NNW GSN +LP E
Sbjct: 363 HASDGVNLKNWSICNMEIFNNWT----GSNPSVLPIYE 396
>At1g10730.1 68414.m01223 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 428
Score = 27.9 bits (59), Expect = 6.1
Identities = 30/110 (27%), Positives = 47/110 (42%), Gaps = 6/110 (5%)
Frame = -3
Query: 463 TFLAPDGSFTLV--RLASGLCP-ITVAKFPEARARRPLSPIFSSRLHTMVPSGIAPTGIT 293
+F+ PDGSF L+ RL++ + P I V E +R + + +R S I
Sbjct: 257 SFIPPDGSFDLMTYRLSTQVKPLIWVEAHIERHSRSRVEMLVKARSQFKDRSYATSVEIE 316
Query: 292 FPTSRVAFFPQ*TIGLCRASGAMKSSFLVLNL---YGSRKCTTAKGAPLP 152
P A+ P L A+ A + LV + YG+++ T LP
Sbjct: 317 LPVPTDAYNPDVRTSLGSAAYAPEKDALVWKIQYFYGNKEHTLKADFHLP 366
>At4g33410.1 68417.m04748 signal peptide peptidase family protein
contains Pfam domain PF04258: Membrane protein of
unknown function (DUF435)
Length = 372
Score = 27.5 bits (58), Expect = 8.1
Identities = 9/13 (69%), Positives = 11/13 (84%)
Frame = -1
Query: 558 YLWYALPAFKIGL 520
Y+WYALP + IGL
Sbjct: 301 YIWYALPGYAIGL 313
>At3g16950.1 68416.m02166 dihydrolipoamide dehydrogenase 1,
plastidic / lipoamide dehydrogenase 1 (PTLPD1) identical
to plastidic lipoamide dehydrogenase from Arabidopsis
thaliana [gi:7159282]
Length = 570
Score = 27.5 bits (58), Expect = 8.1
Identities = 12/38 (31%), Positives = 18/38 (47%)
Frame = -2
Query: 395 GEVSRGTCQTTSITHFLFKIAHNGTLRHSSNRHHISNF 282
G+V GTC +A +G +R N HH+ +F
Sbjct: 117 GDVVGGTCVNRGCVPSKALLAVSGRMRELQNEHHMKSF 154
>At1g68530.2 68414.m07829 very-long-chain fatty acid condensing
enzyme (CUT1) identical to very-long-chain fatty acid
condensing enzyme (CUT1) GB:AF129511 (required for
cuticular wax biosynthesis and pollen fertility:
Millar,A.A., et al., Plant Cell (1999))
Length = 377
Score = 27.5 bits (58), Expect = 8.1
Identities = 19/65 (29%), Positives = 30/65 (46%)
Frame = -3
Query: 643 LMGTTTVRMLYRVHGNTTYIWPAVTFDLVLVVCPSSFQNRFVNTSPPATIPTMPLLLDGR 464
+M V +L +W ++ FDLV V+C SSF F++T + P L+D
Sbjct: 37 IMAIVAVELLRMGPEEILNVWNSLQFDLVQVLC-SSFFVIFISTVYFMSKPRTIYLVDYS 95
Query: 463 TFLAP 449
+ P
Sbjct: 96 CYKPP 100
>At1g68530.1 68414.m07828 very-long-chain fatty acid condensing
enzyme (CUT1) identical to very-long-chain fatty acid
condensing enzyme (CUT1) GB:AF129511 (required for
cuticular wax biosynthesis and pollen fertility:
Millar,A.A., et al., Plant Cell (1999))
Length = 497
Score = 27.5 bits (58), Expect = 8.1
Identities = 19/65 (29%), Positives = 30/65 (46%)
Frame = -3
Query: 643 LMGTTTVRMLYRVHGNTTYIWPAVTFDLVLVVCPSSFQNRFVNTSPPATIPTMPLLLDGR 464
+M V +L +W ++ FDLV V+C SSF F++T + P L+D
Sbjct: 37 IMAIVAVELLRMGPEEILNVWNSLQFDLVQVLC-SSFFVIFISTVYFMSKPRTIYLVDYS 95
Query: 463 TFLAP 449
+ P
Sbjct: 96 CYKPP 100
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,930,568
Number of Sequences: 28952
Number of extensions: 357225
Number of successful extensions: 992
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 961
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 986
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1344285648
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -