Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00576
         (620 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAPJ698.03c |prp12|sap130|U2 snRNP-associated protein Sap130 |S...   100   1e-22
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce...    27   2.2  
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1...    26   3.8  
SPAC12B10.14c |ppk2||serine/threonine protein kinase Ppk2 |Schiz...    26   5.1  
SPBC646.15c |||Pex16 family protein|Schizosaccharomyces pombe|ch...    25   8.8  
SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyc...    25   8.8  
SPAC13A11.04c |ubp8||ubiquitin C-terminal hydrolase Ubp8|Schizos...    25   8.8  

>SPAPJ698.03c |prp12|sap130|U2 snRNP-associated protein Sap130
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1206

 Score =  100 bits (240), Expect = 1e-22
 Identities = 48/90 (53%), Positives = 62/90 (68%)
 Frame = +1

Query: 268  GSLGVLLPFTSREDHDFFQHLEMHMRSENSPLCGRDHLSFRSYYYPVKNVIDGDLCEQFN 447
            G++GV  PF ++ED  FFQ LE  +R E  PL GRDHL++RSYY PVK VIDGDLCE + 
Sbjct: 1114 GTVGVFTPFINQEDVRFFQQLEFLLRKECPPLAGRDHLAYRSYYAPVKCVIDGDLCEMYY 1173

Query: 448  SLDPGKQKAIAGDLERTPAEDQKNLKTFEL 537
            SL    Q+ IA +L+RT AE  K ++ F +
Sbjct: 1174 SLPHPVQEMIANELDRTIAEVSKKIEDFRV 1203



 Score = 65.7 bits (153), Expect = 5e-12
 Identities = 29/78 (37%), Positives = 48/78 (61%)
 Frame = +2

Query: 11   ILDYDTIAVSDKFGNVAIMRLPQSVSDDVDEDPTGNKALWDRGLLNGASQKGDVVVNFHV 190
            ++DYDT+A  DKFGN+ ++R P+ VS   DE+ + +K + ++  LN    K D++ +F  
Sbjct: 1028 LVDYDTLAGGDKFGNIWLLRCPEHVSKLADEENSESKLIHEKPFLNSTPHKLDLMAHFFT 1087

Query: 191  GETVTSLQRATLIPGGSE 244
             +  TSLQ+  L+ G  E
Sbjct: 1088 NDIPTSLQKVQLVEGARE 1105


>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1036

 Score = 27.1 bits (57), Expect = 2.2
 Identities = 18/43 (41%), Positives = 23/43 (53%), Gaps = 5/43 (11%)
 Frame = -3

Query: 201 TVSPT*KLT-----TTSPFCEAPLSKPLSQSALFPVGSSSTSS 88
           TV+PT   T     TTS    + +S PLS S+ +P   SST S
Sbjct: 706 TVAPTSTFTSSGFNTTSGLPTSSVSTPLSNSSAYPSSGSSTFS 748


>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 800

 Score = 26.2 bits (55), Expect = 3.8
 Identities = 18/64 (28%), Positives = 31/64 (48%), Gaps = 2/64 (3%)
 Frame = -3

Query: 267 TNCCNSSASEPPGINVALCREV--TVSPT*KLTTTSPFCEAPLSKPLSQSALFPVGSSST 94
           T+C  S++  P G + +L   +  TV PT   +T+ P      S   + S+  P  S+S 
Sbjct: 170 TSCTTSTSIPPTGGSSSLSTPITPTVPPTSTSSTSIPIPPTSTSSTDTNSSPLPTTSTSC 229

Query: 93  SSLT 82
           ++ T
Sbjct: 230 TTST 233



 Score = 25.0 bits (52), Expect = 8.8
 Identities = 19/64 (29%), Positives = 32/64 (50%), Gaps = 2/64 (3%)
 Frame = -3

Query: 267 TNCCNSSASEPPGINVALCREV--TVSPT*KLTTTSPFCEAPLSKPLSQSALFPVGSSST 94
           T+C  S++  P G + +L   +  TV PT   +T+ P    P S   + +   P+ ++ST
Sbjct: 113 TSCTTSTSIPPTGGSSSLSTPITPTVPPTSTSSTSIPI--PPTSTSSTDTNSNPLPTTST 170

Query: 93  SSLT 82
           S  T
Sbjct: 171 SCTT 174


>SPAC12B10.14c |ppk2||serine/threonine protein kinase Ppk2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 665

 Score = 25.8 bits (54), Expect = 5.1
 Identities = 11/35 (31%), Positives = 22/35 (62%)
 Frame = +1

Query: 298 SREDHDFFQHLEMHMRSENSPLCGRDHLSFRSYYY 402
           S++     + + +H+RSE++P+    HL ++SY Y
Sbjct: 143 SKKSSTLSRSISLHLRSESAPISLPIHL-YKSYSY 176


>SPBC646.15c |||Pex16 family protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 376

 Score = 25.0 bits (52), Expect = 8.8
 Identities = 13/32 (40%), Positives = 19/32 (59%), Gaps = 1/32 (3%)
 Frame = +1

Query: 133 QRFT*WCFTKGRCCGQFS-CW*NCNLSTESNI 225
           Q F  W FT+GR   +F+  W N +LS  ++I
Sbjct: 294 QHFVVWAFTQGRFYDEFTKHWINRSLSWVNSI 325


>SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 987

 Score = 25.0 bits (52), Expect = 8.8
 Identities = 12/34 (35%), Positives = 22/34 (64%)
 Frame = -3

Query: 144 SKPLSQSALFPVGSSSTSSLTDCGNRIIATLPNL 43
           S PLS  ++ PV ++ +SSL++  N ++ T  +L
Sbjct: 151 SLPLSGCSIGPVRTNHSSSLSNSSNSLLQTESSL 184


>SPAC13A11.04c |ubp8||ubiquitin C-terminal hydrolase
           Ubp8|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 449

 Score = 25.0 bits (52), Expect = 8.8
 Identities = 10/28 (35%), Positives = 19/28 (67%)
 Frame = +3

Query: 114 ETKHFGTEVYLMVLHKREMLWSIFMLVK 197
           E++   ++ YL+  H+R++L+S  M VK
Sbjct: 419 ESEVLNSQAYLLFYHERQILYSDEMTVK 446


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,485,913
Number of Sequences: 5004
Number of extensions: 51216
Number of successful extensions: 138
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 127
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 138
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 273658928
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)