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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00576
         (620 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g55220.1 68416.m06133 splicing factor, putative contains CPSF...   124   4e-29
At3g55200.1 68416.m06131 splicing factor, putative contains CPSF...   124   4e-29
At1g19680.1 68414.m02453 expressed protein                             29   2.5  
At1g65080.1 68414.m07378 OXA1 family protein contains Pfam PF020...    28   5.7  
At1g35240.1 68414.m04370 transcriptional factor B3 family protei...    27   7.6  

>At3g55220.1 68416.m06133 splicing factor, putative contains CPSF A
            subunit region (PF03178); contains weak WD-40 repeat
            (PF00400); similar to Splicing factor 3B subunit 3
            (SF3b130)/spliceosomal protein/Splicing factor 3B subunit
            3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens,
            EMBL:HSAJ1443_1
          Length = 1214

 Score =  124 bits (300), Expect = 4e-29
 Identities = 57/94 (60%), Positives = 70/94 (74%)
 Frame = +1

Query: 244  STTIATISGSLGVLLPFTSREDHDFFQHLEMHMRSENSPLCGRDHLSFRSYYYPVKNVID 423
            S    T+ GS+G L  FTSR+D DFF HLEMHMR E  PLCGRDH+++RS Y+PVK+VID
Sbjct: 1114 SIMYGTVMGSIGALHAFTSRDDVDFFSHLEMHMRQEYPPLCGRDHMAYRSAYFPVKDVID 1173

Query: 424  GDLCEQFNSLDPGKQKAIAGDLERTPAEDQKNLK 525
            GDLCEQF +L    Q+ IA +L+RTPAE  K L+
Sbjct: 1174 GDLCEQFPTLPMDLQRKIADELDRTPAEILKKLE 1207



 Score =  107 bits (257), Expect = 6e-24
 Identities = 45/78 (57%), Positives = 63/78 (80%)
 Frame = +2

Query: 14   LDYDTIAVSDKFGNVAIMRLPQSVSDDVDEDPTGNKALWDRGLLNGASQKGDVVVNFHVG 193
            +D+DT+A +DKFGNV  +RLPQ +S++++EDPTG K  W++G LNGA  K D +V FHVG
Sbjct: 1037 VDFDTMAGADKFGNVYFVRLPQDLSEEIEEDPTGGKIKWEQGKLNGAPNKVDEIVQFHVG 1096

Query: 194  ETVTSLQRATLIPGGSEA 247
            + VT LQ+A++IPGGSE+
Sbjct: 1097 DVVTCLQKASMIPGGSES 1114


>At3g55200.1 68416.m06131 splicing factor, putative contains CPSF A
            subunit region (PF03178); contains weak WD-40 repeat
            (PF00400); similar to Splicing factor 3B subunit 3
            (SF3b130)/spliceosomal protein/Splicing factor 3B subunit
            3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens,
            EMBL:HSAJ1443_1
          Length = 1214

 Score =  124 bits (300), Expect = 4e-29
 Identities = 57/94 (60%), Positives = 70/94 (74%)
 Frame = +1

Query: 244  STTIATISGSLGVLLPFTSREDHDFFQHLEMHMRSENSPLCGRDHLSFRSYYYPVKNVID 423
            S    T+ GS+G L  FTSR+D DFF HLEMHMR E  PLCGRDH+++RS Y+PVK+VID
Sbjct: 1114 SIMYGTVMGSIGALHAFTSRDDVDFFSHLEMHMRQEYPPLCGRDHMAYRSAYFPVKDVID 1173

Query: 424  GDLCEQFNSLDPGKQKAIAGDLERTPAEDQKNLK 525
            GDLCEQF +L    Q+ IA +L+RTPAE  K L+
Sbjct: 1174 GDLCEQFPTLPMDLQRKIADELDRTPAEILKKLE 1207



 Score =  107 bits (257), Expect = 6e-24
 Identities = 45/78 (57%), Positives = 63/78 (80%)
 Frame = +2

Query: 14   LDYDTIAVSDKFGNVAIMRLPQSVSDDVDEDPTGNKALWDRGLLNGASQKGDVVVNFHVG 193
            +D+DT+A +DKFGNV  +RLPQ +S++++EDPTG K  W++G LNGA  K D +V FHVG
Sbjct: 1037 VDFDTMAGADKFGNVYFVRLPQDLSEEIEEDPTGGKIKWEQGKLNGAPNKVDEIVQFHVG 1096

Query: 194  ETVTSLQRATLIPGGSEA 247
            + VT LQ+A++IPGGSE+
Sbjct: 1097 DVVTCLQKASMIPGGSES 1114


>At1g19680.1 68414.m02453 expressed protein
          Length = 444

 Score = 29.1 bits (62), Expect = 2.5
 Identities = 16/53 (30%), Positives = 21/53 (39%)
 Frame = -3

Query: 219 ALCREVTVSPT*KLTTTSPFCEAPLSKPLSQSALFPVGSSSTSSLTDCGNRII 61
           A C E   + T K   T P C       LS+ AL        +S   C NR++
Sbjct: 305 AACLETMTNETEKYDPTCPICTETQVTKLSRKALKAEAELKATSYKRCKNRVV 357


>At1g65080.1 68414.m07378 OXA1 family protein contains Pfam PF02096:
           60Kd inner membrane protein; similar to AtOXA1
           (GI:6624207) [Arabidopsis thaliana]
          Length = 525

 Score = 27.9 bits (59), Expect = 5.7
 Identities = 13/39 (33%), Positives = 19/39 (48%)
 Frame = -3

Query: 168 SPFCEAPLSKPLSQSALFPVGSSSTSSLTDCGNRIIATL 52
           SP C +PL + LS    FP+GS       D    ++A +
Sbjct: 46  SPTCFSPLHRLLSSGPEFPIGSQEIIPTDDSSLPVLAVV 84


>At1g35240.1 68414.m04370 transcriptional factor B3 family protein /
           auxin-responsive factor AUX/IAA-related contains Pfam
           profiles: PF02309 AUX/IAA family, PF02362: B3 DNA
           binding domain
          Length = 615

 Score = 27.5 bits (58), Expect = 7.6
 Identities = 13/36 (36%), Positives = 17/36 (47%), Gaps = 2/36 (5%)
 Frame = +1

Query: 358 PLCGRDHLSFRSYYYPVKNV--IDGDLCEQFNSLDP 459
           PLC    L    YY+P  N+  +D    E+ N L P
Sbjct: 35  PLCDIPKLGENVYYFPQGNIELVDASTREELNELQP 70


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,687,318
Number of Sequences: 28952
Number of extensions: 261545
Number of successful extensions: 590
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 577
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 588
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1255974912
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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