Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00548
(747 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 315 3e-88
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 311 4e-87
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 196 1e-52
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 168 6e-44
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 33 0.003
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.3
DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450 monoo... 23 3.0
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 3.0
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 4.0
AB244761-1|BAE66603.1| 504|Apis mellifera cystathionine beta-sy... 22 7.0
AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic ac... 21 9.3
AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase pro... 21 9.3
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 315 bits (773), Expect = 3e-88
Identities = 150/154 (97%), Positives = 151/154 (98%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 232 DKLKAERDVGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 411
DKLKAER+ GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT
Sbjct: 61 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 120
Query: 412 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVTK 513
GEFEAGISKNGQTREHALLAFTLGVKQLIVGV K
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK 154
Score = 141 bits (341), Expect = 7e-36
Identities = 68/81 (83%), Positives = 72/81 (88%), Gaps = 1/81 (1%)
Frame = +3
Query: 507 NKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMLGSR 686
NKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE S+KM +
Sbjct: 153 NKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFK 212
Query: 687 DGS-ERKEGKADGKCLIEALD 746
+ ERKEGK +GKCLIEALD
Sbjct: 213 GWTVERKEGKVEGKCLIEALD 233
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 311 bits (764), Expect = 4e-87
Identities = 148/154 (96%), Positives = 150/154 (97%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 232 DKLKAERDVGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 411
DKLKAER+ GITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG
Sbjct: 61 DKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGI 120
Query: 412 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVTK 513
GEFEAGISKNGQTREHALLAFTLGVKQLIVGV K
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK 154
Score = 129 bits (312), Expect = 2e-32
Identities = 63/81 (77%), Positives = 67/81 (82%), Gaps = 1/81 (1%)
Frame = +3
Query: 507 NKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMLGSR 686
NKMD T+PPYSE RFEEIKKEVSSYIKKIGYN A+VAFVPISGWHGDNMLEPS K +
Sbjct: 153 NKMDMTDPPYSEARFEEIKKEVSSYIKKIGYNTASVAFVPISGWHGDNMLEPSPKTPWYK 212
Query: 687 DGS-ERKEGKADGKCLIEALD 746
ERK+G ADGK LIEALD
Sbjct: 213 GWKVERKDGNADGKTLIEALD 233
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 196 bits (479), Expect = 1e-52
Identities = 94/97 (96%), Positives = 95/97 (97%)
Frame = +1
Query: 223 WVLDKLKAERDVGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 402
WVLDKLKAER+ GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA
Sbjct: 1 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60
Query: 403 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVTK 513
AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGV K
Sbjct: 61 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK 97
Score = 141 bits (341), Expect = 7e-36
Identities = 68/81 (83%), Positives = 72/81 (88%), Gaps = 1/81 (1%)
Frame = +3
Query: 507 NKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMLGSR 686
NKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE S+KM +
Sbjct: 96 NKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFK 155
Query: 687 DGS-ERKEGKADGKCLIEALD 746
+ ERKEGK +GKCLIEALD
Sbjct: 156 GWTVERKEGKVEGKCLIEALD 176
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 168 bits (408), Expect = 6e-44
Identities = 80/81 (98%), Positives = 80/81 (98%)
Frame = +1
Query: 271 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 450
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 451 REHALLAFTLGVKQLIVGVTK 513
REHALLAFTLGVKQLIVGV K
Sbjct: 61 REHALLAFTLGVKQLIVGVNK 81
Score = 141 bits (341), Expect = 7e-36
Identities = 68/81 (83%), Positives = 72/81 (88%), Gaps = 1/81 (1%)
Frame = +3
Query: 507 NKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMLGSR 686
NKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE S+KM +
Sbjct: 80 NKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFK 139
Query: 687 DGS-ERKEGKADGKCLIEALD 746
+ ERKEGK +GKCLIEALD
Sbjct: 140 GWTVERKEGKVEGKCLIEALD 160
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 33.1 bits (72), Expect = 0.003
Identities = 16/35 (45%), Positives = 18/35 (51%)
Frame = +1
Query: 310 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 414
VT +D PGH FI G D VL+VAA G
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDG 229
Score = 25.4 bits (53), Expect = 0.57
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +1
Query: 64 KTHINIVVIGHVDSGKST 117
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.2 bits (50), Expect = 1.3
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +1
Query: 73 INIVVIGHVDSGKST 117
INI IGHV GKST
Sbjct: 43 INIGTIGHVAHGKST 57
>DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450
monooxygenase protein.
Length = 517
Score = 23.0 bits (47), Expect = 3.0
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = -2
Query: 65 FSLPIFG*SRITNCV*Y 15
FSLPIFG I +C+ Y
Sbjct: 57 FSLPIFGTRWIFSCIGY 73
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 23.0 bits (47), Expect = 3.0
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +1
Query: 295 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 396
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.6 bits (46), Expect = 4.0
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +1
Query: 295 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 396
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
>AB244761-1|BAE66603.1| 504|Apis mellifera cystathionine
beta-synthase protein.
Length = 504
Score = 21.8 bits (44), Expect = 7.0
Identities = 11/44 (25%), Positives = 20/44 (45%)
Frame = +2
Query: 560 QEGSILIHQEDWLQPSCCRFRAHFWMARRQHVGAFNQNAWFKGW 691
+E ++I D ++ +F + +WM R + QN K W
Sbjct: 324 EEKRMVIILPDGIRNYLTKFVSEYWMETRGFLQPVCQNEMNKWW 367
>AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic
acetylcholine Apisa7-2 subunit protein.
Length = 461
Score = 21.4 bits (43), Expect = 9.3
Identities = 8/24 (33%), Positives = 13/24 (54%)
Frame = -2
Query: 671 FG*RLQHVVSVPSRNGHESDSSWV 600
FG L H++ V +N + + WV
Sbjct: 35 FGLSLHHIIDVDEKNQILTTNCWV 58
>AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase
protein.
Length = 580
Score = 21.4 bits (43), Expect = 9.3
Identities = 7/20 (35%), Positives = 13/20 (65%)
Frame = +1
Query: 514 WIPLNHHTVSPDLRKSRRKY 573
W+P+N + S +L +R+Y
Sbjct: 443 WLPVNENYKSLNLAAQKREY 462
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 209,278
Number of Sequences: 438
Number of extensions: 4438
Number of successful extensions: 29
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 20
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 23388480
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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