Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00544
(797 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q16TL0 Cluster: Putative uncharacterized protein; n=1; ... 70 6e-11
UniRef50_UPI00015B6430 Cluster: PREDICTED: similar to conserved ... 68 2e-10
UniRef50_Q9W494 Cluster: CG12410-PA; n=2; Sophophora|Rep: CG1241... 63 7e-09
UniRef50_P54356 Cluster: Protein twisted gastrulation precursor;... 43 0.008
UniRef50_UPI00015B454A Cluster: PREDICTED: similar to conserved ... 41 0.041
UniRef50_Q9GZX9 Cluster: Twisted gastrulation protein homolog 1 ... 41 0.041
UniRef50_UPI0000F2147D Cluster: PREDICTED: similar to Twsg1b pro... 38 0.39
UniRef50_Q6IMD6 Cluster: Twisted gastrulation 2; n=5; Xenopus|Re... 36 1.6
UniRef50_Q0PL68 Cluster: Twisted gastrulation; n=1; Saccoglossus... 36 1.6
UniRef50_Q4LE75 Cluster: CENPE variant protein; n=9; Euteleostom... 34 4.8
UniRef50_Q02224 Cluster: Centromeric protein E; n=8; Eutheria|Re... 34 4.8
UniRef50_A0E169 Cluster: Chromosome undetermined scaffold_72, wh... 33 6.3
UniRef50_Q1DML3 Cluster: Predicted protein; n=1; Coccidioides im... 33 6.3
UniRef50_UPI000069EC4E Cluster: GAS2-like protein 1 (Growth arre... 33 8.3
UniRef50_A3Q4U9 Cluster: Putative uncharacterized protein; n=3; ... 33 8.3
UniRef50_Q4P259 Cluster: Putative uncharacterized protein; n=1; ... 33 8.3
>UniRef50_Q16TL0 Cluster: Putative uncharacterized protein; n=1;
Aedes aegypti|Rep: Putative uncharacterized protein -
Aedes aegypti (Yellowfever mosquito)
Length = 159
Score = 70.1 bits (164), Expect = 6e-11
Identities = 34/81 (41%), Positives = 42/81 (51%)
Frame = +1
Query: 274 VALICVVPIAYACNEAICASVVSKCMLTQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVD 453
V LIC+ AY+CNE +CAS+VSKCMLTQ ++
Sbjct: 16 VGLICLAQFAYSCNEMVCASIVSKCMLTQSCKCDMKNCSCCKECSECLSYLYTECCSCLE 75
Query: 454 MCPKPNDTQTELSKTSYVGSL 516
MCPKPN+T ELSK S+V L
Sbjct: 76 MCPKPNETTNELSKQSHVEEL 96
Score = 43.6 bits (98), Expect = 0.006
Identities = 18/49 (36%), Positives = 29/49 (59%)
Frame = +3
Query: 519 DGVPGLFAALTSDPDAQQRWLSMTYPVDIDLSAYRPVPEKQVVYHLQSV 665
+G P LF LTS+ D ++RW T+P+D+D + Y E + Y + S+
Sbjct: 97 EGFPNLFNVLTSEEDPEERWRIFTFPIDLDAAMYGSNAETGMKYLMLSL 145
>UniRef50_UPI00015B6430 Cluster: PREDICTED: similar to conserved
hypothetical protein; n=1; Nasonia vitripennis|Rep:
PREDICTED: similar to conserved hypothetical protein -
Nasonia vitripennis
Length = 377
Score = 68.1 bits (159), Expect = 2e-10
Identities = 30/79 (37%), Positives = 46/79 (58%), Gaps = 2/79 (2%)
Frame = +3
Query: 528 PGLFAALTSDPDAQQRWLSMTYPVDIDLSAYRPVPEKQVVYHLQSVEQ--DSEPVNTDMV 701
P LF AL +PD +RW S TYP+ + P EK Y++QS ++ +S+ V
Sbjct: 232 PELFRALNEEPDEHKRWNSFTYPISLPNRILSPQQEKNFEYYMQSADKKYNSKTVKLQAA 291
Query: 702 TFNCTVAYMSQCMSCDKCR 758
T NCTV+++++C S KC+
Sbjct: 292 TTNCTVSFLAECNSWSKCK 310
Score = 52.8 bits (121), Expect = 1e-05
Identities = 30/70 (42%), Positives = 32/70 (45%), Gaps = 1/70 (1%)
Frame = +1
Query: 301 AYACNEAICASVVSKCMLTQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVDMCPKPNDTQ 480
AY CNEAICAS VSKCML D+CPK NDTQ
Sbjct: 155 AYGCNEAICASTVSKCMLINSCKCDLTTCSCCKDCFLCLSLLYDECCSCFDLCPKSNDTQ 214
Query: 481 T-ELSKTSYV 507
T LSK S+V
Sbjct: 215 TVPLSKKSHV 224
>UniRef50_Q9W494 Cluster: CG12410-PA; n=2; Sophophora|Rep:
CG12410-PA - Drosophila melanogaster (Fruit fly)
Length = 257
Score = 63.3 bits (147), Expect = 7e-09
Identities = 29/80 (36%), Positives = 41/80 (51%)
Frame = +1
Query: 268 LIVALICVVPIAYACNEAICASVVSKCMLTQXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 447
+++A++C +CNE +CAS+VSKCMLTQ
Sbjct: 13 VLLAIVCFYGTVESCNEVVCASIVSKCMLTQSCKCELKNCSCCKECLKCLGKNYEECCSC 72
Query: 448 VDMCPKPNDTQTELSKTSYV 507
V++CPKPNDT+ LSK S+V
Sbjct: 73 VELCPKPNDTRNSLSKKSHV 92
Score = 52.8 bits (121), Expect = 1e-05
Identities = 30/86 (34%), Positives = 45/86 (52%), Gaps = 6/86 (6%)
Frame = +3
Query: 519 DGVPGLFAALTSDPDAQQ---RWLSMTYPVDIDLSAYRPVPEKQVVYHLQSVEQDSEPV- 686
DGVP LF A+ + + W T+ VD D P EK Y L++ +++ +
Sbjct: 96 DGVPELFNAVATPDEGDSFGYNWNVFTFQVDFDKYLKGPKLEKDGHYFLRTNDKNLDEAI 155
Query: 687 --NTDMVTFNCTVAYMSQCMSCDKCR 758
++VT NCTV Y+ QC+S +KCR
Sbjct: 156 QERDNIVTVNCTVIYLDQCVSWNKCR 181
>UniRef50_P54356 Cluster: Protein twisted gastrulation precursor;
n=2; Sophophora|Rep: Protein twisted gastrulation
precursor - Drosophila melanogaster (Fruit fly)
Length = 249
Score = 43.2 bits (97), Expect = 0.008
Identities = 21/69 (30%), Positives = 28/69 (40%)
Frame = +1
Query: 310 CNEAICASVVSKCMLTQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVDMCPKPNDTQTEL 489
CNE +C SVVSKC++TQ +DMCPK D L
Sbjct: 26 CNEVVCGSVVSKCLITQSCQCKLNDCHCCKDCLNCLGELYIECCGCLDMCPKHKDVLPSL 85
Query: 490 SKTSYVGSL 516
+ S +G +
Sbjct: 86 TPRSEIGDI 94
>UniRef50_UPI00015B454A Cluster: PREDICTED: similar to conserved
hypothetical protein; n=1; Nasonia vitripennis|Rep:
PREDICTED: similar to conserved hypothetical protein -
Nasonia vitripennis
Length = 229
Score = 40.7 bits (91), Expect = 0.041
Identities = 23/69 (33%), Positives = 28/69 (40%), Gaps = 1/69 (1%)
Frame = +1
Query: 304 YACNEAICASVVSKCMLTQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVDMCPKPNDTQT 483
Y CNEA+CAS VSKCML ++CP N T+T
Sbjct: 50 YGCNEAVCASTVSKCMLLNSCKCDLSTCSCCKDCFLCLSFLFDEYCSCFNLCPNSNVTET 109
Query: 484 -ELSKTSYV 507
L K S+V
Sbjct: 110 ISLGKKSHV 118
>UniRef50_Q9GZX9 Cluster: Twisted gastrulation protein homolog 1
precursor; n=23; Euteleostomi|Rep: Twisted gastrulation
protein homolog 1 precursor - Homo sapiens (Human)
Length = 223
Score = 40.7 bits (91), Expect = 0.041
Identities = 29/89 (32%), Positives = 43/89 (48%), Gaps = 6/89 (6%)
Frame = +3
Query: 510 ELGDGVPGLFAALTSDPDAQQRWLSMTYPVDIDLSAYRPVP---EKQVVYHLQSVEQDSE 680
EL + +P LF ALT + D Q W +++PV +LS + + E H Q+V S
Sbjct: 95 ELHEPIPSLFRALT-EGDTQLNWNIVSFPVAEELSHHENLVSFLETVNQPHHQNVSVPSN 153
Query: 681 PVNTDMVTFN---CTVAYMSQCMSCDKCR 758
V+ + CTV Y CMS +C+
Sbjct: 154 NVHAPYSSDKEHMCTVVYFDDCMSIHQCK 182
>UniRef50_UPI0000F2147D Cluster: PREDICTED: similar to Twsg1b
protein, partial; n=1; Danio rerio|Rep: PREDICTED:
similar to Twsg1b protein, partial - Danio rerio
Length = 261
Score = 37.5 bits (83), Expect = 0.39
Identities = 15/33 (45%), Positives = 25/33 (75%)
Frame = +1
Query: 262 SYLIVALICVVPIAYACNEAICASVVSKCMLTQ 360
S +++ L+ + IA ACN+A+CAS VSKC++ +
Sbjct: 197 SSVVLLLLSSLSIALACNKALCASDVSKCLIQE 229
>UniRef50_Q6IMD6 Cluster: Twisted gastrulation 2; n=5; Xenopus|Rep:
Twisted gastrulation 2 - Xenopus laevis (African clawed
frog)
Length = 248
Score = 35.5 bits (78), Expect = 1.6
Identities = 16/31 (51%), Positives = 22/31 (70%), Gaps = 1/31 (3%)
Frame = +1
Query: 271 IVALIC-VVPIAYACNEAICASVVSKCMLTQ 360
I AL+C V + CN+A+CAS VSKC+L +
Sbjct: 31 ISALLCSTVTLVTGCNKALCASDVSKCLLQE 61
>UniRef50_Q0PL68 Cluster: Twisted gastrulation; n=1; Saccoglossus
kowalevskii|Rep: Twisted gastrulation - Saccoglossus
kowalevskii (Acorn worm)
Length = 205
Score = 35.5 bits (78), Expect = 1.6
Identities = 16/35 (45%), Positives = 22/35 (62%), Gaps = 1/35 (2%)
Frame = +1
Query: 259 RSYLIVALIC-VVPIAYACNEAICASVVSKCMLTQ 360
R L +C + + +ACNEA+CAS VSKC L +
Sbjct: 7 RVLLFTLSVCYAIYLVHACNEAMCASDVSKCQLME 41
>UniRef50_Q4LE75 Cluster: CENPE variant protein; n=9;
Euteleostomi|Rep: CENPE variant protein - Homo sapiens
(Human)
Length = 2585
Score = 33.9 bits (74), Expect = 4.8
Identities = 16/30 (53%), Positives = 23/30 (76%), Gaps = 1/30 (3%)
Frame = -1
Query: 422 ELRQLKQSLQHEQS-FKSHLHDWVNMHFDT 336
+L+QL++SLQ E+ KS +HD VNM+ DT
Sbjct: 913 DLKQLQESLQIERDQLKSDIHDTVNMNIDT 942
>UniRef50_Q02224 Cluster: Centromeric protein E; n=8; Eutheria|Rep:
Centromeric protein E - Homo sapiens (Human)
Length = 2663
Score = 33.9 bits (74), Expect = 4.8
Identities = 16/30 (53%), Positives = 23/30 (76%), Gaps = 1/30 (3%)
Frame = -1
Query: 422 ELRQLKQSLQHEQS-FKSHLHDWVNMHFDT 336
+L+QL++SLQ E+ KS +HD VNM+ DT
Sbjct: 933 DLKQLQESLQIERDQLKSDIHDTVNMNIDT 962
>UniRef50_A0E169 Cluster: Chromosome undetermined scaffold_72, whole
genome shotgun sequence; n=1; Paramecium
tetraurelia|Rep: Chromosome undetermined scaffold_72,
whole genome shotgun sequence - Paramecium tetraurelia
Length = 653
Score = 33.5 bits (73), Expect = 6.3
Identities = 15/40 (37%), Positives = 27/40 (67%)
Frame = -1
Query: 449 TQLQHSLYKELRQLKQSLQHEQSFKSHLHDWVNMHFDTTL 330
TQLQ+SL+K+L +L Q+ ++ ++ + LH W+N D +
Sbjct: 241 TQLQYSLFKKLTELDQTKRY-RADQGLLHPWLNEQIDVEI 279
>UniRef50_Q1DML3 Cluster: Predicted protein; n=1; Coccidioides
immitis|Rep: Predicted protein - Coccidioides immitis
Length = 141
Score = 33.5 bits (73), Expect = 6.3
Identities = 17/43 (39%), Positives = 26/43 (60%), Gaps = 1/43 (2%)
Frame = -2
Query: 562 SGSLVRAAKRPGTPSPSSQHRKFL-IVLSACHLVWDTYRRNYS 437
SG R A RP + S + +HRKFL V S+CH +D + + ++
Sbjct: 29 SGHTTRPAARPHSKSQNREHRKFLRNVRSSCHRCFDHHTQPHT 71
>UniRef50_UPI000069EC4E Cluster: GAS2-like protein 1 (Growth
arrest-specific 2-like 1) (GAS2-related protein on
chromosome 22) (GAR22 protein).; n=1; Xenopus
tropicalis|Rep: GAS2-like protein 1 (Growth
arrest-specific 2-like 1) (GAS2-related protein on
chromosome 22) (GAR22 protein). - Xenopus tropicalis
Length = 632
Score = 33.1 bits (72), Expect = 8.3
Identities = 13/37 (35%), Positives = 23/37 (62%)
Frame = -1
Query: 449 TQLQHSLYKELRQLKQSLQHEQSFKSHLHDWVNMHFD 339
+Q+Q S K +R + S ++ ++ K L DW+NM +D
Sbjct: 5 SQIQSSASKSIRPFRSSEEYLEAMKEDLTDWLNMLYD 41
>UniRef50_A3Q4U9 Cluster: Putative uncharacterized protein; n=3;
Mycobacterium|Rep: Putative uncharacterized protein -
Mycobacterium sp. (strain JLS)
Length = 457
Score = 33.1 bits (72), Expect = 8.3
Identities = 17/52 (32%), Positives = 29/52 (55%)
Frame = +3
Query: 489 IKNFLCWELGDGVPGLFAALTSDPDAQQRWLSMTYPVDIDLSAYRPVPEKQV 644
++ F C LG G+ G + P + RW++ T PVD+D++A +P + V
Sbjct: 46 LRRFHC-NLGHGLLGRLVERSPLPFGRHRWVASTGPVDLDIAA-ADLPRRDV 95
>UniRef50_Q4P259 Cluster: Putative uncharacterized protein; n=1;
Ustilago maydis|Rep: Putative uncharacterized protein -
Ustilago maydis (Smut fungus)
Length = 1614
Score = 33.1 bits (72), Expect = 8.3
Identities = 15/52 (28%), Positives = 25/52 (48%)
Frame = -1
Query: 347 HFDTTLAHIASLHAYAIGTTQISATIK*DLLTWQDLMKQSKNA*KINNDQTS 192
HFD A + L + IG T + + L+ W ++ SKN N+D+ +
Sbjct: 1147 HFDIQSASLIDLPGWRIGRTLHATRVAHALMNWSEMFSGSKNGCHTNSDEAA 1198
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 730,426,870
Number of Sequences: 1657284
Number of extensions: 13677140
Number of successful extensions: 36969
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 35517
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 36945
length of database: 575,637,011
effective HSP length: 99
effective length of database: 411,565,895
effective search space used: 68319938570
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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