Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00535
(790 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 240 2e-64
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 237 2e-63
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 120 2e-28
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 105 1e-23
SPBC23G7.13c |||urea transporter |Schizosaccharomyces pombe|chr ... 27 4.0
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 7.1
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.1
SPAPB1A11.01 ||SPAPB24D3.11|membrane transporter|Schizosaccharom... 25 9.4
SPAC19G12.02c |pms1||MutL family mismatch-repair protein Pms1|Sc... 25 9.4
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 240 bits (587), Expect = 2e-64
Identities = 116/214 (54%), Positives = 139/214 (64%), Gaps = 2/214 (0%)
Frame = +3
Query: 96 QCISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHV 275
+ IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK+V
Sbjct: 3 EIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYV 62
Query: 276 PRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRI 455
PR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D+I
Sbjct: 63 PRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKI 122
Query: 456 RKLADQCTGLQGFLIFHS--SVEVPALGSLPY*WSVSPLTTARSLNWXXXXXXXXXXXXX 629
R++AD C+GLQGFL+FHS G+L T L +
Sbjct: 123 RRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVV 182
Query: 630 XXXXXXXXXXXXXXXXHSDCAFMVDNEAIYDICR 731
+DC FMVDNE+ YDICR
Sbjct: 183 EPYNSVLTTHATLDL--ADCTFMVDNESCYDICR 214
Score = 70.9 bits (166), Expect = 2e-13
Identities = 36/71 (50%), Positives = 46/71 (64%)
Frame = +2
Query: 548 MERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSIXXXXXXXXXXXXCFHGRQ*SHL*HL 727
+ERL+++Y KKSKL+F++YPAPQVST+VVEPYNS+ F S
Sbjct: 154 LERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDIC 213
Query: 728 PRNLDIERPTY 760
RNLDIERP+Y
Sbjct: 214 RRNLDIERPSY 224
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 237 bits (579), Expect = 2e-63
Identities = 117/217 (53%), Positives = 140/217 (64%), Gaps = 5/217 (2%)
Frame = +3
Query: 96 QCISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSETG 260
+ ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSETG
Sbjct: 3 EVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSETG 61
Query: 261 AGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDL 440
GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D
Sbjct: 62 QGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDS 121
Query: 441 VLDRIRKLADQCTGLQGFLIFHSSVEVPALGSLPY*WSVSPLTTARSLNWXXXXXXXXXX 620
VL+RIR++AD C+GLQGFL+FHS G + + N
Sbjct: 122 VLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQV 181
Query: 621 XXXXXXXXXXXXXXXXXXXHSDCAFMVDNEAIYDICR 731
+SDC FMVDNEA YDICR
Sbjct: 182 STSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICR 218
Score = 72.5 bits (170), Expect = 6e-14
Identities = 36/71 (50%), Positives = 46/71 (64%)
Frame = +2
Query: 548 MERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSIXXXXXXXXXXXXCFHGRQ*SHL*HL 727
+ERL+++YGKKS L+F++YPAPQVST+VVEPYNS+ F +
Sbjct: 158 LERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDIC 217
Query: 728 PRNLDIERPTY 760
RNLDIERPTY
Sbjct: 218 RRNLDIERPTY 228
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 120 bits (289), Expect = 2e-28
Identities = 57/138 (41%), Positives = 80/138 (57%)
Frame = +3
Query: 96 QCISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHV 275
+ + + GQ G Q+G A W EHG+ G T + N +F+E GK+V
Sbjct: 3 EIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGKYV 60
Query: 276 PRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRI 455
PRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD +
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 456 RKLADQCTGLQGFLIFHS 509
R+ A+ C LQGF + HS
Sbjct: 121 RREAEACDALQGFQLTHS 138
Score = 31.9 bits (69), Expect = 0.11
Identities = 12/34 (35%), Positives = 21/34 (61%)
Frame = +2
Query: 548 MERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNS 649
+ ++ +Y + F++ PAP+ S VVEPYN+
Sbjct: 152 LSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNA 185
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 105 bits (251), Expect = 1e-23
Identities = 53/140 (37%), Positives = 84/140 (60%), Gaps = 2/140 (1%)
Frame = +3
Query: 96 QCISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHV 275
+ I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ +++
Sbjct: 4 EIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRYI 61
Query: 276 PRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVLD 449
PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++D
Sbjct: 62 PRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMD 120
Query: 450 RIRKLADQCTGLQGFLIFHS 509
I + AD L+GF + HS
Sbjct: 121 MIDREADGSDSLEGFSLLHS 140
Score = 32.3 bits (70), Expect = 0.081
Identities = 16/36 (44%), Positives = 24/36 (66%), Gaps = 1/36 (2%)
Frame = +2
Query: 548 MERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYNSI 652
+ERL+ Y KK ++++P Q VS VV+PYNS+
Sbjct: 154 LERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSL 189
>SPBC23G7.13c |||urea transporter |Schizosaccharomyces pombe|chr
2|||Manual
Length = 664
Score = 26.6 bits (56), Expect = 4.0
Identities = 13/53 (24%), Positives = 27/53 (50%)
Frame = +1
Query: 64 SIEKNSNQNACSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCPQTRPSGV 222
S++K +N+N S T + ++ + ++W+ AS+L+ +T GV
Sbjct: 32 SLKKFNNENQTSEHFNTASHSVRTGLVASAVVSSWTWASTLLTSAQKTYQYGV 84
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 7.1
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +1
Query: 169 STASSLMARCPQTRPSGVETILSTLSSARPELAST 273
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 7.1
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +2
Query: 188 WPDAHRQDHRGWRRFFQHFLQR 253
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPAPB1A11.01 ||SPAPB24D3.11|membrane
transporter|Schizosaccharomyces pombe|chr 1|||Manual
Length = 495
Score = 25.4 bits (53), Expect = 9.4
Identities = 7/20 (35%), Positives = 13/20 (65%)
Frame = +1
Query: 502 STLRWRYRLWVHFLIDGASL 561
S++ WR+ W+ ++ G SL
Sbjct: 190 SSISWRWEFWILLMLSGVSL 209
>SPAC19G12.02c |pms1||MutL family mismatch-repair protein
Pms1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 794
Score = 25.4 bits (53), Expect = 9.4
Identities = 14/48 (29%), Positives = 23/48 (47%)
Frame = +1
Query: 73 KNSNQNACSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCPQTRPS 216
KNS QN C + + ++ S+S+ S S+ S PQ+ P+
Sbjct: 324 KNSLQNLCESCGHAISCSRSQSIFSYSSQIPDSSGDSTDQELPQSIPA 371
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,282,099
Number of Sequences: 5004
Number of extensions: 66906
Number of successful extensions: 219
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 196
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 210
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 383374054
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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