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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00530
         (797 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr...    83   3e-17
SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 3|...    60   5e-10
SPBC530.08 |||transcription factor |Schizosaccharomyces pombe|ch...    32   0.11 
SPBC211.08c |||ribonuclease PH-like|Schizosaccharomyces pombe|ch...    25   9.5  
SPAC6F6.06c |rax2||cell polarity factor Rax2|Schizosaccharomyces...    25   9.5  
SPAC1D4.06c |csk1||cyclin-dependent kinase activating kinase Csk...    25   9.5  
SPBC17D1.06 |dbp3||ATP-dependent RNA helicase Dbp3 |Schizosaccha...    25   9.5  

>SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 591

 Score = 83.4 bits (197), Expect = 3e-17
 Identities = 42/82 (51%), Positives = 52/82 (63%), Gaps = 5/82 (6%)
 Frame = +1

Query: 541 FFKDTDKLRRGDIIGCVGHPGKTKK-----GELSIIPKNIKLLAPCLHMLPHLHFGLKDK 705
           F    + LRRGDIIG  G+PG++       GELSI  +   LL+PCL MLP  H+GLKD 
Sbjct: 168 FAAQHEHLRRGDIIGIRGYPGRSNPKGRADGELSIFARQCVLLSPCLRMLPKEHYGLKDL 227

Query: 706 ETRFRKRYLDLILNDKVRQSFI 771
           E R R+RYLDLI+N   R  F+
Sbjct: 228 EIRHRQRYLDLIMNRSTRDRFV 249



 Score = 83.0 bits (196), Expect = 4e-17
 Identities = 39/84 (46%), Positives = 57/84 (67%)
 Frame = +2

Query: 257 YYKLRSGAVAALKNGLKEDHPYPHKFNVSISLEEFIEKYQNLNNGDVLENVTLSVAGRVH 436
           Y++ RS  +  L+   K+ +PYPHKF V+I+L EFI KY+ L  G+    V ++VAGRV 
Sbjct: 75  YFENRSRTIMELRQ-TKDPNPYPHKFQVTITLPEFIAKYEGLARGETKPEVEVAVAGRVL 133

Query: 437 SIRESGAKLIFYDLRAEGAKIQVM 508
            +R +G KL FY++ A+G K+QVM
Sbjct: 134 GLRTAGNKLRFYEIHADGKKLQVM 157



 Score = 25.4 bits (53), Expect = 9.5
 Identities = 7/28 (25%), Positives = 17/28 (60%)
 Frame = +3

Query: 630 YPEKYKIACTMPSYVAAFTFRSQRQRNP 713
           YP K+++  T+P ++A +   ++ +  P
Sbjct: 95  YPHKFQVTITLPEFIAKYEGLARGETKP 122


>SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 531

 Score = 59.7 bits (138), Expect = 5e-10
 Identities = 32/77 (41%), Positives = 46/77 (59%), Gaps = 1/77 (1%)
 Frame = +1

Query: 526 ETEDKFFKDTDKLRRGDIIGCVGHPGKTKKGELSIIPKNI-KLLAPCLHMLPHLHFGLKD 702
           E + KF      L++GD I C G+ G++  GELSI    + KLL+PCLH +P     L +
Sbjct: 126 EMKGKFIPRLKALQKGDCIQCSGNVGRSGSGELSIYATELPKLLSPCLHPIP---VKLTN 182

Query: 703 KETRFRKRYLDLILNDK 753
            E RF KR++D++ N K
Sbjct: 183 YEKRFEKRFVDMMSNTK 199



 Score = 39.9 bits (89), Expect = 4e-04
 Identities = 20/49 (40%), Positives = 30/49 (61%)
 Frame = +2

Query: 362 IEKYQNLNNGDVLENVTLSVAGRVHSIRESGAKLIFYDLRAEGAKIQVM 508
           IEK++N      +  V  +V GR+ SIR SG+KL F+D+     K+QV+
Sbjct: 67  IEKWRNKITKSEIAMVRYTVCGRISSIRYSGSKLAFFDVLYGNKKLQVV 115


>SPBC530.08 |||transcription factor |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 815

 Score = 31.9 bits (69), Expect = 0.11
 Identities = 20/72 (27%), Positives = 36/72 (50%)
 Frame = +2

Query: 521 YMKQKISFLKILINYAEVISLVVWAIQVKLRKENFLLSRKI*NCLHHAFICCRIYISVSK 700
           Y   ++   + L++Y E  S  + A++V  R+  F L+ K   C+  A +C +    +SK
Sbjct: 494 YYSFRMLIYRPLLHYLEADSPAMQALKVPDRQTAFTLACK---CVDSAIVCVQNLSHLSK 550

Query: 701 TKKPALEKGIWT 736
             K  L++  WT
Sbjct: 551 GLKRTLDRYYWT 562


>SPBC211.08c |||ribonuclease PH-like|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 257

 Score = 25.4 bits (53), Expect = 9.5
 Identities = 13/32 (40%), Positives = 18/32 (56%), Gaps = 2/32 (6%)
 Frame = +3

Query: 621 TFYYPEKYKIACTM--PSYVAAFTFRSQRQRN 710
           +++  EK KIACT+  P     F FRS  + N
Sbjct: 52  SYFESEKIKIACTVSGPRPSKTFAFRSSAKLN 83


>SPAC6F6.06c |rax2||cell polarity factor Rax2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1155

 Score = 25.4 bits (53), Expect = 9.5
 Identities = 17/58 (29%), Positives = 26/58 (44%), Gaps = 3/58 (5%)
 Frame = +2

Query: 257 YYKLRSGAVAALKNGLK---EDHPYPHKFNVSISLEEFIEKYQNLNNGDVLENVTLSV 421
           Y  L SG +    +  +   E  PY +   V+ S+    + Y N++NG V  N T  V
Sbjct: 467 YVSLYSGYLQGFSDNFRPYVELLPYKNSRMVTHSIRFLEQSYTNVSNGLVFVNTTTDV 524


>SPAC1D4.06c |csk1||cyclin-dependent kinase activating kinase Csk1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 306

 Score = 25.4 bits (53), Expect = 9.5
 Identities = 8/13 (61%), Positives = 12/13 (92%)
 Frame = -3

Query: 108 NFLPWLSDLIHLT 70
           +F+PWL+D+ HLT
Sbjct: 6   HFVPWLTDIRHLT 18


>SPBC17D1.06 |dbp3||ATP-dependent RNA helicase Dbp3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 578

 Score = 25.4 bits (53), Expect = 9.5
 Identities = 12/31 (38%), Positives = 18/31 (58%)
 Frame = +2

Query: 296 NGLKEDHPYPHKFNVSISLEEFIEKYQNLNN 388
           NGL ++   P    VS + E  I+ Y+NLN+
Sbjct: 228 NGLSDNKSVPRVLVVSPTRELAIQTYENLNS 258


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,951,982
Number of Sequences: 5004
Number of extensions: 56941
Number of successful extensions: 174
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 164
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 172
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 389395636
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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