BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00530 (797 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr... 83 3e-17 SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 3|... 60 5e-10 SPBC530.08 |||transcription factor |Schizosaccharomyces pombe|ch... 32 0.11 SPBC211.08c |||ribonuclease PH-like|Schizosaccharomyces pombe|ch... 25 9.5 SPAC6F6.06c |rax2||cell polarity factor Rax2|Schizosaccharomyces... 25 9.5 SPAC1D4.06c |csk1||cyclin-dependent kinase activating kinase Csk... 25 9.5 SPBC17D1.06 |dbp3||ATP-dependent RNA helicase Dbp3 |Schizosaccha... 25 9.5 >SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 2|||Manual Length = 591 Score = 83.4 bits (197), Expect = 3e-17 Identities = 42/82 (51%), Positives = 52/82 (63%), Gaps = 5/82 (6%) Frame = +1 Query: 541 FFKDTDKLRRGDIIGCVGHPGKTKK-----GELSIIPKNIKLLAPCLHMLPHLHFGLKDK 705 F + LRRGDIIG G+PG++ GELSI + LL+PCL MLP H+GLKD Sbjct: 168 FAAQHEHLRRGDIIGIRGYPGRSNPKGRADGELSIFARQCVLLSPCLRMLPKEHYGLKDL 227 Query: 706 ETRFRKRYLDLILNDKVRQSFI 771 E R R+RYLDLI+N R F+ Sbjct: 228 EIRHRQRYLDLIMNRSTRDRFV 249 Score = 83.0 bits (196), Expect = 4e-17 Identities = 39/84 (46%), Positives = 57/84 (67%) Frame = +2 Query: 257 YYKLRSGAVAALKNGLKEDHPYPHKFNVSISLEEFIEKYQNLNNGDVLENVTLSVAGRVH 436 Y++ RS + L+ K+ +PYPHKF V+I+L EFI KY+ L G+ V ++VAGRV Sbjct: 75 YFENRSRTIMELRQ-TKDPNPYPHKFQVTITLPEFIAKYEGLARGETKPEVEVAVAGRVL 133 Query: 437 SIRESGAKLIFYDLRAEGAKIQVM 508 +R +G KL FY++ A+G K+QVM Sbjct: 134 GLRTAGNKLRFYEIHADGKKLQVM 157 Score = 25.4 bits (53), Expect = 9.5 Identities = 7/28 (25%), Positives = 17/28 (60%) Frame = +3 Query: 630 YPEKYKIACTMPSYVAAFTFRSQRQRNP 713 YP K+++ T+P ++A + ++ + P Sbjct: 95 YPHKFQVTITLPEFIAKYEGLARGETKP 122 >SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 3|||Manual Length = 531 Score = 59.7 bits (138), Expect = 5e-10 Identities = 32/77 (41%), Positives = 46/77 (59%), Gaps = 1/77 (1%) Frame = +1 Query: 526 ETEDKFFKDTDKLRRGDIIGCVGHPGKTKKGELSIIPKNI-KLLAPCLHMLPHLHFGLKD 702 E + KF L++GD I C G+ G++ GELSI + KLL+PCLH +P L + Sbjct: 126 EMKGKFIPRLKALQKGDCIQCSGNVGRSGSGELSIYATELPKLLSPCLHPIP---VKLTN 182 Query: 703 KETRFRKRYLDLILNDK 753 E RF KR++D++ N K Sbjct: 183 YEKRFEKRFVDMMSNTK 199 Score = 39.9 bits (89), Expect = 4e-04 Identities = 20/49 (40%), Positives = 30/49 (61%) Frame = +2 Query: 362 IEKYQNLNNGDVLENVTLSVAGRVHSIRESGAKLIFYDLRAEGAKIQVM 508 IEK++N + V +V GR+ SIR SG+KL F+D+ K+QV+ Sbjct: 67 IEKWRNKITKSEIAMVRYTVCGRISSIRYSGSKLAFFDVLYGNKKLQVV 115 >SPBC530.08 |||transcription factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 815 Score = 31.9 bits (69), Expect = 0.11 Identities = 20/72 (27%), Positives = 36/72 (50%) Frame = +2 Query: 521 YMKQKISFLKILINYAEVISLVVWAIQVKLRKENFLLSRKI*NCLHHAFICCRIYISVSK 700 Y ++ + L++Y E S + A++V R+ F L+ K C+ A +C + +SK Sbjct: 494 YYSFRMLIYRPLLHYLEADSPAMQALKVPDRQTAFTLACK---CVDSAIVCVQNLSHLSK 550 Query: 701 TKKPALEKGIWT 736 K L++ WT Sbjct: 551 GLKRTLDRYYWT 562 >SPBC211.08c |||ribonuclease PH-like|Schizosaccharomyces pombe|chr 2|||Manual Length = 257 Score = 25.4 bits (53), Expect = 9.5 Identities = 13/32 (40%), Positives = 18/32 (56%), Gaps = 2/32 (6%) Frame = +3 Query: 621 TFYYPEKYKIACTM--PSYVAAFTFRSQRQRN 710 +++ EK KIACT+ P F FRS + N Sbjct: 52 SYFESEKIKIACTVSGPRPSKTFAFRSSAKLN 83 >SPAC6F6.06c |rax2||cell polarity factor Rax2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1155 Score = 25.4 bits (53), Expect = 9.5 Identities = 17/58 (29%), Positives = 26/58 (44%), Gaps = 3/58 (5%) Frame = +2 Query: 257 YYKLRSGAVAALKNGLK---EDHPYPHKFNVSISLEEFIEKYQNLNNGDVLENVTLSV 421 Y L SG + + + E PY + V+ S+ + Y N++NG V N T V Sbjct: 467 YVSLYSGYLQGFSDNFRPYVELLPYKNSRMVTHSIRFLEQSYTNVSNGLVFVNTTTDV 524 >SPAC1D4.06c |csk1||cyclin-dependent kinase activating kinase Csk1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 306 Score = 25.4 bits (53), Expect = 9.5 Identities = 8/13 (61%), Positives = 12/13 (92%) Frame = -3 Query: 108 NFLPWLSDLIHLT 70 +F+PWL+D+ HLT Sbjct: 6 HFVPWLTDIRHLT 18 >SPBC17D1.06 |dbp3||ATP-dependent RNA helicase Dbp3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 578 Score = 25.4 bits (53), Expect = 9.5 Identities = 12/31 (38%), Positives = 18/31 (58%) Frame = +2 Query: 296 NGLKEDHPYPHKFNVSISLEEFIEKYQNLNN 388 NGL ++ P VS + E I+ Y+NLN+ Sbjct: 228 NGLSDNKSVPRVLVVSPTRELAIQTYENLNS 258 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,951,982 Number of Sequences: 5004 Number of extensions: 56941 Number of successful extensions: 174 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 164 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 172 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 389395636 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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