Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00530
         (797 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g11710.1 68416.m01435 lysyl-tRNA synthetase, putative / lysin...    85   4e-17
At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N) f...    60   2e-09
At5g45370.3 68418.m05573 nodulin-related / integral membrane fam...    30   2.0  
At3g50030.1 68416.m05470 hypothetical protein                          29   4.7  
At3g52170.1 68416.m05727 expressed protein                             28   6.2  

>At3g11710.1 68416.m01435 lysyl-tRNA synthetase, putative /
           lysine--tRNA ligase, putative similar to SP|Q43776
           Lysyl-tRNA synthetase (EC 6.1.1.6) (Lysine--tRNA ligase)
           {Lycopersicon esculentum}; contains Pfam profile
           PF00152: tRNA synthetases class II (D, K and N)
          Length = 626

 Score = 85.4 bits (202), Expect = 4e-17
 Identities = 43/84 (51%), Positives = 58/84 (69%)
 Frame = +2

Query: 257 YYKLRSGAVAALKNGLKEDHPYPHKFNVSISLEEFIEKYQNLNNGDVLENVTLSVAGRVH 436
           YY+ R   +AA K   K ++PYPHKF VS+S+ ++IE Y +LNNGD +EN   S+AGR+ 
Sbjct: 90  YYENRLKYLAAEK--AKGENPYPHKFAVSMSIPKYIETYGSLNNGDHVENAEESLAGRIM 147

Query: 437 SIRESGAKLIFYDLRAEGAKIQVM 508
           S R S +KL FYDL  +  K+QVM
Sbjct: 148 SKRSSSSKLFFYDLHGDDFKVQVM 171



 Score = 76.2 bits (179), Expect = 2e-14
 Identities = 43/100 (43%), Positives = 60/100 (60%), Gaps = 21/100 (21%)
 Frame = +1

Query: 532 EDKFFKDTDKLRRGDIIGCVGHPGKTKKGELSIIPKNIKLLAPCLHMLPHL--------- 684
           E +F K     +RGDI+G +G PGKTK+GELSI P++  LL+ CLHM+P           
Sbjct: 181 EAEFLKLHSNAKRGDIVGVIGFPGKTKRGELSIFPRSFILLSHCLHMMPRKADNVNAKKP 240

Query: 685 ------------HFGLKDKETRFRKRYLDLILNDKVRQSF 768
                        + LKD+E+R+R+R+LD+ILN +VRQ F
Sbjct: 241 EIWVPGQTRNPEAYVLKDQESRYRQRHLDMILNVEVRQIF 280


>At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N)
           family protein similar to SP|Q9RHV9 Lysyl-tRNA
           synthetase (EC 6.1.1.6) (Lysine--tRNA ligase) {Bacillus
           stearothermophilus}; contains Pfam profile: PF00152 tRNA
           synthetases class II (D, K and N)
          Length = 602

 Score = 59.7 bits (138), Expect = 2e-09
 Identities = 31/85 (36%), Positives = 49/85 (57%)
 Frame = +1

Query: 529 TEDKFFKDTDKLRRGDIIGCVGHPGKTKKGELSIIPKNIKLLAPCLHMLPHLHFGLKDKE 708
           ++D+F +    +  GDI+G  G   +T+KGELSI   +  +L   L  LP  + GL D +
Sbjct: 173 SDDQFEQLKQFIDIGDILGASGSMKRTEKGELSICVNSFSILTKSLLPLPDKYHGLTDID 232

Query: 709 TRFRKRYLDLILNDKVRQSFIQELK 783
            R+R+RY+D+I N +V   F +  K
Sbjct: 233 KRYRQRYVDMIANPEVADVFRRRAK 257



 Score = 39.1 bits (87), Expect = 0.003
 Identities = 28/90 (31%), Positives = 47/90 (52%), Gaps = 3/90 (3%)
 Frame = +2

Query: 317 PYPHKFNVSISLEEFIEKYQNLNNGDVLENV--TLSVAGRVHSIRESGAKLIFYDLRAEG 490
           PY +K+  S S  +  E Y++L NG+  +N    +S+AGRV + R  G KL F  LR + 
Sbjct: 102 PYAYKWEKSHSANQLQEIYKHLANGEESDNEIDCVSIAGRVVARRAFG-KLAFLTLRDDS 160

Query: 491 AKIQVMLMQNYMK-QKISFLKILINYAEVI 577
             IQ+   +  +   +   LK  I+  +++
Sbjct: 161 GTIQLYCEKERLSDDQFEQLKQFIDIGDIL 190


>At5g45370.3 68418.m05573 nodulin-related / integral membrane family
           protein contains Pfam profile:PF00892 integral membrane
           protein DUF6
          Length = 321

 Score = 29.9 bits (64), Expect = 2.0
 Identities = 12/26 (46%), Positives = 18/26 (69%)
 Frame = +3

Query: 48  CIFISILRLSELSPIAMAESSTEKIS 125
           C+F  +L LS L+P+A    STEK++
Sbjct: 52  CVFRDLLALSILAPLAFFRESTEKVN 77


>At3g50030.1 68416.m05470 hypothetical protein 
          Length = 501

 Score = 28.7 bits (61), Expect = 4.7
 Identities = 16/64 (25%), Positives = 33/64 (51%)
 Frame = +2

Query: 443 RESGAKLIFYDLRAEGAKIQVMLMQNYMKQKISFLKILINYAEVISLVVWAIQVKLRKEN 622
           +E+    +   L+    ++  +++Q+Y K K S LK+    A      +W I+V+ +K+ 
Sbjct: 286 KETALDTLLLLLKDSNVRVAQIVLQDYHKIKYSGLKMTTEEAHRSIENLWEIKVERKKKE 345

Query: 623 FLLS 634
            L+S
Sbjct: 346 KLMS 349


>At3g52170.1 68416.m05727 expressed protein
          Length = 499

 Score = 28.3 bits (60), Expect = 6.2
 Identities = 16/41 (39%), Positives = 23/41 (56%), Gaps = 2/41 (4%)
 Frame = +2

Query: 350 LEEFIEKYQNLNNGDV--LENVTLSVAGRVHSIRESGAKLI 466
           +E FI+K+Q LNNG    L      V G  ++IRE   ++I
Sbjct: 39  VESFIKKHQKLNNGSFPSLSLTHKEVGGSFYTIREIVREII 79


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,939,521
Number of Sequences: 28952
Number of extensions: 280472
Number of successful extensions: 642
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 619
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 642
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1804564000
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)