Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00527
(736 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_16632| Best HMM Match : HSP20 (HMM E-Value=1e-12) 74 1e-13
SB_21425| Best HMM Match : HSP20 (HMM E-Value=1.9e-26) 66 2e-11
SB_38963| Best HMM Match : HSP20 (HMM E-Value=7.3e-26) 62 6e-10
SB_37349| Best HMM Match : HSP20 (HMM E-Value=7.3e-26) 62 6e-10
SB_21424| Best HMM Match : HSP20 (HMM E-Value=0) 60 2e-09
SB_3291| Best HMM Match : No HMM Matches (HMM E-Value=.) 60 2e-09
SB_58906| Best HMM Match : HSP20 (HMM E-Value=3e-26) 58 6e-09
SB_49292| Best HMM Match : HSP20 (HMM E-Value=1.9e-29) 58 6e-09
SB_58907| Best HMM Match : HSP20 (HMM E-Value=4.2e-05) 44 1e-04
SB_6186| Best HMM Match : HSP20 (HMM E-Value=1.1e-06) 36 0.026
SB_24406| Best HMM Match : DUF536 (HMM E-Value=1.5) 31 0.73
SB_13144| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 1.7
SB_1830| Best HMM Match : DUF331 (HMM E-Value=3.5) 30 1.7
SB_75| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 3.9
SB_44943| Best HMM Match : Peptidase_A17 (HMM E-Value=2e-06) 29 5.2
SB_54295| Best HMM Match : RVP (HMM E-Value=0.037) 28 9.0
>SB_16632| Best HMM Match : HSP20 (HMM E-Value=1e-12)
Length = 210
Score = 73.7 bits (173), Expect = 1e-13
Identities = 35/89 (39%), Positives = 58/89 (65%), Gaps = 1/89 (1%)
Frame = +1
Query: 244 RGSSLGSSIKSDKDKFQV-NLDVQHFAPEEISVKTADGYIVVEGKHEEKKDQHGYISRQF 420
R SL ++ + + DK ++ LDV+++ PEEIS+K G I ++GKH+ + +HGY + +F
Sbjct: 25 RNCSLAANTEMEGDKVEIATLDVKNYRPEEISLKVEHGRIKIDGKHKSE-GEHGYETSEF 83
Query: 421 TRRYALPEGCTAESVESRLSSDGVLSVIA 507
R Y LP+G +V SR++ DG+L + A
Sbjct: 84 HRSYNLPDGVDVSTVSSRITGDGLLHIEA 112
Score = 25.4 bits (53), Expect(2) = 1.0
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = +1
Query: 253 SLGSSIKSDKDKFQVNLDVQHFAPE 327
S G K D +F V LDV F+P+
Sbjct: 131 SAGDIAKIDDKRFTVTLDVSSFSPD 155
Score = 24.2 bits (50), Expect(2) = 1.0
Identities = 11/28 (39%), Positives = 16/28 (57%)
Frame = +1
Query: 424 RRYALPEGCTAESVESRLSSDGVLSVIA 507
R + LP+ +S+ SRL DG L + A
Sbjct: 161 RHFVLPKDVDMDSLVSRLGKDGKLYIEA 188
>SB_21425| Best HMM Match : HSP20 (HMM E-Value=1.9e-26)
Length = 185
Score = 66.5 bits (155), Expect = 2e-11
Identities = 34/78 (43%), Positives = 49/78 (62%), Gaps = 1/78 (1%)
Frame = +1
Query: 277 DKDKFQV-NLDVQHFAPEEISVKTADGYIVVEGKHEEKKDQHGYISRQFTRRYALPEGCT 453
D DKFQ+ LDV+ F PEEI+ K +G I V G + ++ G+ S++F R Y LPEG
Sbjct: 2 DADKFQIATLDVREFKPEEITCKVENGKIKVSGLQRHESEE-GFDSKEFRRCYNLPEGVD 60
Query: 454 AESVESRLSSDGVLSVIA 507
S+ +R++ DG+L V A
Sbjct: 61 ESSISTRIAEDGMLHVEA 78
Score = 54.0 bits (124), Expect = 1e-07
Identities = 31/85 (36%), Positives = 45/85 (52%), Gaps = 1/85 (1%)
Frame = +1
Query: 277 DKDKFQVNLDVQHFAPEEISVKTADGYIVVEGKHEEKKDQHGYIS-RQFTRRYALPEGCT 453
D KF + LDV F PEE+ VK + V + E ++HG+ + RQF R + LP
Sbjct: 96 DDTKFTLALDVSDFKPEEVDVKVYGHELSVRARQE--CEEHGFFTARQFNRHFVLPREVD 153
Query: 454 AESVESRLSSDGVLSVIAPGRCRQQ 528
+++ RL DGVL + A R +Q
Sbjct: 154 MDTLVPRLGKDGVLYIEADKRPLRQ 178
>SB_38963| Best HMM Match : HSP20 (HMM E-Value=7.3e-26)
Length = 190
Score = 61.7 bits (143), Expect = 6e-10
Identities = 29/79 (36%), Positives = 53/79 (67%), Gaps = 1/79 (1%)
Frame = +1
Query: 274 SDKDKFQV-NLDVQHFAPEEISVKTADGYIVVEGKHEEKKDQHGYISRQFTRRYALPEGC 450
++++K ++ LDV+ + PEEIS K +G++ V+G+H + G+ ++F R +ALPEG
Sbjct: 2 ANENKLEIAKLDVREYRPEEISFKVENGFVKVQGRH-VNEGPFGFELKEFRRTFALPEGV 60
Query: 451 TAESVESRLSSDGVLSVIA 507
A +V++R+S+ G L + A
Sbjct: 61 EASNVKTRISNHGQLHIEA 79
Score = 49.6 bits (113), Expect = 3e-06
Identities = 27/90 (30%), Positives = 42/90 (46%)
Frame = +1
Query: 247 GSSLGSSIKSDKDKFQVNLDVQHFAPEEISVKTADGYIVVEGKHEEKKDQHGYISRQFTR 426
G G DKF + +DV F P+ I V+ ++V HE + + H Y + F R
Sbjct: 85 GPDGGEEESKGDDKFSMAMDVTGFPPDSIKVQVLGNELLVSANHEVEHEGH-YHAMHFNR 143
Query: 427 RYALPEGCTAESVESRLSSDGVLSVIAPGR 516
++ LP +S+ +RL +G L A R
Sbjct: 144 QFVLPREVDMDSLTTRLDKEGKLHFEAKKR 173
>SB_37349| Best HMM Match : HSP20 (HMM E-Value=7.3e-26)
Length = 190
Score = 61.7 bits (143), Expect = 6e-10
Identities = 29/79 (36%), Positives = 53/79 (67%), Gaps = 1/79 (1%)
Frame = +1
Query: 274 SDKDKFQV-NLDVQHFAPEEISVKTADGYIVVEGKHEEKKDQHGYISRQFTRRYALPEGC 450
++++K ++ LDV+ + PEEIS K +G++ V+G+H + G+ ++F R +ALPEG
Sbjct: 2 ANENKLEIAKLDVREYRPEEISFKVENGFVKVQGRH-VNEGPFGFELKEFRRTFALPEGV 60
Query: 451 TAESVESRLSSDGVLSVIA 507
A +V++R+S+ G L + A
Sbjct: 61 EASNVKTRISNHGQLHIEA 79
Score = 49.6 bits (113), Expect = 3e-06
Identities = 27/90 (30%), Positives = 42/90 (46%)
Frame = +1
Query: 247 GSSLGSSIKSDKDKFQVNLDVQHFAPEEISVKTADGYIVVEGKHEEKKDQHGYISRQFTR 426
G G DKF + +DV F P+ I V+ ++V HE + + H Y + F R
Sbjct: 85 GPDGGEEESKGDDKFSMAMDVTGFPPDSIKVQVLGNELLVSANHEVEHEGH-YHAMHFNR 143
Query: 427 RYALPEGCTAESVESRLSSDGVLSVIAPGR 516
++ LP +S+ +RL +G L A R
Sbjct: 144 QFVLPREVDMDSLTTRLDKEGKLHFEAKKR 173
>SB_21424| Best HMM Match : HSP20 (HMM E-Value=0)
Length = 424
Score = 60.1 bits (139), Expect = 2e-09
Identities = 28/79 (35%), Positives = 52/79 (65%), Gaps = 1/79 (1%)
Frame = +1
Query: 274 SDKDKFQV-NLDVQHFAPEEISVKTADGYIVVEGKHEEKKDQHGYISRQFTRRYALPEGC 450
++++K ++ LDV+ + PEEIS K +G++ V+G+H + G+ ++F R + LPEG
Sbjct: 2 ANENKLEIAKLDVREYRPEEISFKVENGFVKVQGRH-VNEGPFGFELKEFRRTFTLPEGV 60
Query: 451 TAESVESRLSSDGVLSVIA 507
A +V++R+S+ G L + A
Sbjct: 61 EASNVKTRISNHGQLHIEA 79
Score = 59.3 bits (137), Expect = 3e-09
Identities = 28/76 (36%), Positives = 49/76 (64%), Gaps = 1/76 (1%)
Frame = +1
Query: 283 DKFQV-NLDVQHFAPEEISVKTADGYIVVEGKHEEKKDQHGYISRQFTRRYALPEGCTAE 459
D+ ++ LDV+ + PEEIS K +G++ V+G+H + G+ ++F R + LPEG A
Sbjct: 239 DELEIAKLDVREYRPEEISFKVENGFVKVQGRH-VNEGPFGFELKEFRRTFTLPEGVEAS 297
Query: 460 SVESRLSSDGVLSVIA 507
+V++R+S+ G L + A
Sbjct: 298 NVKTRISNHGQLHIEA 313
Score = 49.6 bits (113), Expect = 3e-06
Identities = 27/90 (30%), Positives = 42/90 (46%)
Frame = +1
Query: 247 GSSLGSSIKSDKDKFQVNLDVQHFAPEEISVKTADGYIVVEGKHEEKKDQHGYISRQFTR 426
G G DKF + +DV F P+ I V+ ++V HE + + H Y + F R
Sbjct: 85 GPDGGEEESKGDDKFSMAMDVTGFPPDSIKVQVLGNELLVSANHEVEHEGH-YHAMHFNR 143
Query: 427 RYALPEGCTAESVESRLSSDGVLSVIAPGR 516
++ LP +S+ +RL +G L A R
Sbjct: 144 QFVLPREVDMDSLTTRLDKEGKLHFEAKKR 173
Score = 49.6 bits (113), Expect = 3e-06
Identities = 27/90 (30%), Positives = 42/90 (46%)
Frame = +1
Query: 247 GSSLGSSIKSDKDKFQVNLDVQHFAPEEISVKTADGYIVVEGKHEEKKDQHGYISRQFTR 426
G G DKF + +DV F P+ I V+ ++V HE + + H Y + F R
Sbjct: 319 GPDGGEEESKGDDKFSMAMDVTGFPPDSIKVQVLGNELLVSANHEVEHEGH-YHAMHFNR 377
Query: 427 RYALPEGCTAESVESRLSSDGVLSVIAPGR 516
++ LP +S+ +RL +G L A R
Sbjct: 378 QFVLPREVDMDSLTTRLDKEGKLHFEAKKR 407
>SB_3291| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 760
Score = 60.1 bits (139), Expect = 2e-09
Identities = 28/79 (35%), Positives = 52/79 (65%), Gaps = 1/79 (1%)
Frame = +1
Query: 274 SDKDKFQV-NLDVQHFAPEEISVKTADGYIVVEGKHEEKKDQHGYISRQFTRRYALPEGC 450
++++K ++ LDV+ + PEEIS K +G++ V+G+H + G+ ++F R + LPEG
Sbjct: 2 ANENKLEIAKLDVREYRPEEISFKVENGFVKVQGRH-VNEGPFGFELKEFRRTFTLPEGV 60
Query: 451 TAESVESRLSSDGVLSVIA 507
A +V++R+S+ G L + A
Sbjct: 61 EASNVKTRISNHGQLHIEA 79
Score = 49.6 bits (113), Expect = 3e-06
Identities = 27/90 (30%), Positives = 42/90 (46%)
Frame = +1
Query: 247 GSSLGSSIKSDKDKFQVNLDVQHFAPEEISVKTADGYIVVEGKHEEKKDQHGYISRQFTR 426
G G DKF + +DV F P+ I V+ ++V HE + + H Y + F R
Sbjct: 85 GPDGGEEESKGDDKFSMAMDVTGFPPDSIKVQVLGNELLVSANHEVEHEGH-YHAMHFNR 143
Query: 427 RYALPEGCTAESVESRLSSDGVLSVIAPGR 516
++ LP +S+ +RL +G L A R
Sbjct: 144 QFVLPREVDMDSLTTRLDKEGKLHFEAKKR 173
>SB_58906| Best HMM Match : HSP20 (HMM E-Value=3e-26)
Length = 695
Score = 58.4 bits (135), Expect = 6e-09
Identities = 28/79 (35%), Positives = 51/79 (64%), Gaps = 1/79 (1%)
Frame = +1
Query: 274 SDKDKFQV-NLDVQHFAPEEISVKTADGYIVVEGKHEEKKDQHGYISRQFTRRYALPEGC 450
++++K ++ LDV+ + PEEIS K +G + V+G+H + G+ ++F R + LPEG
Sbjct: 3 NNENKLEIAKLDVREYRPEEISFKVENGVVKVQGRH-VNEGPFGFELKEFRRTFTLPEGV 61
Query: 451 TAESVESRLSSDGVLSVIA 507
A +V++R+S+ G L + A
Sbjct: 62 EASNVKTRISNHGQLHIEA 80
Score = 52.8 bits (121), Expect = 3e-07
Identities = 28/86 (32%), Positives = 45/86 (52%)
Frame = +1
Query: 259 GSSIKSDKDKFQVNLDVQHFAPEEISVKTADGYIVVEGKHEEKKDQHGYISRQFTRRYAL 438
G+ +S DKF + +DV+ F PE I V+ ++V HE + + H Y + F R++ L
Sbjct: 89 GAKKESKDDKFSMAMDVKGFPPEAIKVQVLGNELLVSANHEVEHEGH-YHAMHFNRQFIL 147
Query: 439 PEGCTAESVESRLSSDGVLSVIAPGR 516
P +S+ +RL +G L A R
Sbjct: 148 PREVDMDSLTTRLDKEGKLHFEAKKR 173
>SB_49292| Best HMM Match : HSP20 (HMM E-Value=1.9e-29)
Length = 189
Score = 58.4 bits (135), Expect = 6e-09
Identities = 28/69 (40%), Positives = 44/69 (63%)
Frame = +1
Query: 301 LDVQHFAPEEISVKTADGYIVVEGKHEEKKDQHGYISRQFTRRYALPEGCTAESVESRLS 480
LDV+ + PEEIS K +G + V+G+H + + G+ ++F R + LPEG E+V SR+S
Sbjct: 12 LDVKEYRPEEISFKVENGVVKVQGRH-VNEGEFGFELKEFRRTFTLPEGIDPENVTSRIS 70
Query: 481 SDGVLSVIA 507
+ G L + A
Sbjct: 71 NHGHLHIEA 79
Score = 52.0 bits (119), Expect = 5e-07
Identities = 25/75 (33%), Positives = 41/75 (54%)
Frame = +1
Query: 271 KSDKDKFQVNLDVQHFAPEEISVKTADGYIVVEGKHEEKKDQHGYISRQFTRRYALPEGC 450
K+ +DKF + +DV F PE I V+ ++V HE + + H Y + F R++ LP
Sbjct: 92 KAKEDKFSMAIDVAGFPPESIKVQVLGNELLVNANHEVEHEGH-YHAMHFNRQFVLPREV 150
Query: 451 TAESVESRLSSDGVL 495
+S+ +RL +G L
Sbjct: 151 DMDSLTTRLDKEGKL 165
>SB_58907| Best HMM Match : HSP20 (HMM E-Value=4.2e-05)
Length = 259
Score = 44.0 bits (99), Expect = 1e-04
Identities = 23/62 (37%), Positives = 34/62 (54%), Gaps = 1/62 (1%)
Frame = +1
Query: 262 SSIKSDKDKFQVNLDVQHFAPEEISVKTADGYIVVEGKHEEKKDQHGYI-SRQFTRRYAL 438
S+ D KF + LDV F PEE+ VK + V + E ++HG+ +RQF R + L
Sbjct: 19 SAATKDDTKFTLALDVSDFKPEEVDVKVYGHELSVRARQE--CEEHGFFTARQFNRHFVL 76
Query: 439 PE 444
P+
Sbjct: 77 PQ 78
>SB_6186| Best HMM Match : HSP20 (HMM E-Value=1.1e-06)
Length = 237
Score = 36.3 bits (80), Expect = 0.026
Identities = 19/80 (23%), Positives = 46/80 (57%), Gaps = 1/80 (1%)
Frame = +1
Query: 271 KSDKDKFQVNLDVQHFAPEEISVKTADGYIVVEGKHEEKKDQHGYIS-RQFTRRYALPEG 447
++DK+ ++ ++V+ FA +++S++ ++++G+ +K D G+ RQF R P+
Sbjct: 94 QNDKNGIKLEMNVRGFAAKDVSLQVDGQDLIIKGEKMDKDD--GWCKMRQFCWRRRFPKS 151
Query: 448 CTAESVESRLSSDGVLSVIA 507
+S+++ L +L + A
Sbjct: 152 TDMKSIKATLKYGDILEIEA 171
>SB_24406| Best HMM Match : DUF536 (HMM E-Value=1.5)
Length = 436
Score = 31.5 bits (68), Expect = 0.73
Identities = 11/22 (50%), Positives = 18/22 (81%)
Frame = +1
Query: 373 KHEEKKDQHGYISRQFTRRYAL 438
KHEE++D+HGY+ + T ++AL
Sbjct: 72 KHEEREDRHGYLKDKVTLQHAL 93
>SB_13144| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 266
Score = 30.3 bits (65), Expect = 1.7
Identities = 11/36 (30%), Positives = 19/36 (52%)
Frame = +1
Query: 337 VKTADGYIVVEGKHEEKKDQHGYISRQFTRRYALPE 444
+++A GY+V G H K + G + + R LP+
Sbjct: 10 IRSARGYVVTSGSHTSKSEVRGIMEETCSNRNPLPQ 45
>SB_1830| Best HMM Match : DUF331 (HMM E-Value=3.5)
Length = 326
Score = 30.3 bits (65), Expect = 1.7
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = +1
Query: 214 ILPPVASPCCRGSSLGSSIKSDKDKFQVNLDVQHFAPE 327
ILPPV P S LG +KS KD+ ++++D+ +
Sbjct: 273 ILPPVVLPSLASSQLGRGLKSRKDE-KLDVDINQLTKD 309
>SB_75| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 343
Score = 29.1 bits (62), Expect = 3.9
Identities = 11/24 (45%), Positives = 14/24 (58%)
Frame = -2
Query: 561 FGRSESCVHPPLLAAPSWCDYRQH 490
F R+ V+PP LA P W Y +H
Sbjct: 31 FTRTIGKVYPPTLANPDWIHYEEH 54
>SB_44943| Best HMM Match : Peptidase_A17 (HMM E-Value=2e-06)
Length = 295
Score = 28.7 bits (61), Expect = 5.2
Identities = 9/19 (47%), Positives = 13/19 (68%)
Frame = -3
Query: 554 DRNLAFTLHCWRHLPGAIT 498
+R +F CWRH PGA++
Sbjct: 247 ERQSSFDPECWRHCPGAVS 265
>SB_54295| Best HMM Match : RVP (HMM E-Value=0.037)
Length = 239
Score = 27.9 bits (59), Expect = 9.0
Identities = 11/30 (36%), Positives = 20/30 (66%), Gaps = 1/30 (3%)
Frame = +3
Query: 516 VPPAVEGERKIPIAQTGPVRKEVKD-QSNG 602
V P + RK+PI+ GPV++++ + + NG
Sbjct: 156 VTPVIHPPRKLPISPLGPVKEKLSEMEQNG 185
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 22,741,594
Number of Sequences: 59808
Number of extensions: 510981
Number of successful extensions: 1580
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 1407
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1574
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1974037988
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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