Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00527
(736 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 42 6e-04
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 40 0.001
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 36 0.021
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 36 0.028
At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein (H... 35 0.049
At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 33 0.15
At1g78910.1 68414.m09199 pseudouridine synthase family protein l... 29 3.2
At5g11470.1 68418.m01339 bromo-adjacent homology (BAH) domain-co... 29 4.2
At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family prote... 29 4.2
At2g27980.1 68415.m03391 expressed protein 27 9.8
At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containi... 27 9.8
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 41.5 bits (93), Expect = 6e-04
Identities = 27/77 (35%), Positives = 43/77 (55%), Gaps = 8/77 (10%)
Frame = +1
Query: 304 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 459
D+ EE+ V+ DG I+ + G+ +EEK D+ + R +FTRR+ LPE E
Sbjct: 66 DLPGLRKEEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTRRFRLPENAKME 125
Query: 460 SVESRLSSDGVLSVIAP 510
+++ + +GVLSV P
Sbjct: 126 EIKASM-ENGVLSVTVP 141
>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
(HSP17.4-CI) identical to 17.4 kDa class I heat shock
protein SP:P19036 from [Arabidopsis thaliana]
Length = 156
Score = 40.3 bits (90), Expect = 0.001
Identities = 28/77 (36%), Positives = 41/77 (53%), Gaps = 8/77 (10%)
Frame = +1
Query: 304 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 459
DV EE+ V+ DG I+ + G+ +EEK D + R +F RR+ LPE E
Sbjct: 65 DVPGLKKEEVKVEVEDGNILQISGERSSENEEKSDTWHRVERSSGKFMRRFRLPENAKVE 124
Query: 460 SVESRLSSDGVLSVIAP 510
V++ + +GVLSV P
Sbjct: 125 EVKASM-ENGVLSVTVP 140
>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
(HSP18.1-CI) identical to 18.2 kDa class I heat shock
protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
contains Pfam profile: PF00011 Hsp20/alpha crystallin
family
Length = 161
Score = 36.3 bits (80), Expect = 0.021
Identities = 24/77 (31%), Positives = 42/77 (54%), Gaps = 8/77 (10%)
Frame = +1
Query: 304 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 459
D+ EE+ V+ D ++ + G+ +EEK D+ + R +F RR+ LPE E
Sbjct: 68 DLPGLKKEEVKVEVEDKNVLQISGERSKENEEKNDKWHRVERASGKFMRRFRLPENAKME 127
Query: 460 SVESRLSSDGVLSVIAP 510
V++ + +GVL+V+ P
Sbjct: 128 EVKATM-ENGVLTVVVP 143
>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
(HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
crystallin family; identified in Scharf, K-D., et al,
Cell Stress & Chaperones (2001) 6: 225-237.
Length = 153
Score = 35.9 bits (79), Expect = 0.028
Identities = 25/77 (32%), Positives = 41/77 (53%), Gaps = 8/77 (10%)
Frame = +1
Query: 304 DVQHFAPEEISVKTA-DGYIVVEG-KHEEKKDQHGYISR------QFTRRYALPEGCTAE 459
D+ EE+ V+ D + + G +H EK+D++ R QFTRR+ LPE +
Sbjct: 62 DLPGLKKEEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMD 121
Query: 460 SVESRLSSDGVLSVIAP 510
V++ + +GVL+V P
Sbjct: 122 QVKAAM-ENGVLTVTVP 137
>At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein
(HSP22.0-ER) identical to endomembrane-localized small
heat shock protein GI:511795 from [Arabidopsis thaliana]
Length = 195
Score = 35.1 bits (77), Expect = 0.049
Identities = 25/85 (29%), Positives = 47/85 (55%), Gaps = 8/85 (9%)
Frame = +1
Query: 271 KSDKDKFQVNLDVQHFAPEEISVKTAD-GYIVVEG---KHEEKK-DQHGYISR---QFTR 426
K + ++ LD+ +E+ ++ + G + V G + EEKK DQ + R +F R
Sbjct: 76 KETAEGHEIMLDIPGLKKDEVKIEVEENGVLRVSGERKREEEKKGDQWHRVERSYGKFWR 135
Query: 427 RYALPEGCTAESVESRLSSDGVLSV 501
++ LP+ ESV+++L +GVL++
Sbjct: 136 QFKLPDNVDMESVKAKL-ENGVLTI 159
>At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein
(HSP17.8-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]; contains Pfam
PF00011: Hsp20/alpha crystallin family
Length = 157
Score = 33.5 bits (73), Expect = 0.15
Identities = 23/77 (29%), Positives = 41/77 (53%), Gaps = 8/77 (10%)
Frame = +1
Query: 304 DVQHFAPEEISVKTADGYIV-VEGKH----EEKKDQHGYISR---QFTRRYALPEGCTAE 459
D+ EE+ V+ D ++ + G+ EEK+D + R QF+R++ LPE +
Sbjct: 64 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMD 123
Query: 460 SVESRLSSDGVLSVIAP 510
V++ + +GVL+V P
Sbjct: 124 QVKASM-ENGVLTVTVP 139
>At1g78910.1 68414.m09199 pseudouridine synthase family protein low
similarity to SP|P23851 Ribosomal large subunit
pseudouridine synthase C (EC 4.2.1.70) (Pseudouridylate
synthase) (Uracil hydrolyase) {Escherichia coli};
contains Pfam profile PF00849: RNA pseudouridylate
synthase
Length = 478
Score = 29.1 bits (62), Expect = 3.2
Identities = 23/59 (38%), Positives = 26/59 (44%), Gaps = 2/59 (3%)
Frame = -1
Query: 469 TPQIQPYSPPAKRSDE*IDAICNHADLSSPRVCLQPR--CSRPPF*QRFLPGRNAGRPG 299
T I+ YS A I N A L P+ PR S PPF LPG+NAG G
Sbjct: 14 TVLIRRYSRVAPPPSSVIRVTNNVAHLGPPKQGPLPRQLISLPPFPGHPLPGKNAGADG 72
>At5g11470.1 68418.m01339 bromo-adjacent homology (BAH)
domain-containing protein contains Pfam profile PF01426:
BAH domain
Length = 691
Score = 28.7 bits (61), Expect = 4.2
Identities = 18/67 (26%), Positives = 32/67 (47%)
Frame = +1
Query: 241 CRGSSLGSSIKSDKDKFQVNLDVQHFAPEEISVKTADGYIVVEGKHEEKKDQHGYISRQF 420
C+ +S G + +K +Q+ + A EE S K + G H KDQ + +Q
Sbjct: 235 CKENSNGCKEEKEKGHYQLAIKKSTLA-EERSNKDSGS----RGNHYNGKDQESEVKKQL 289
Query: 421 TRRYALP 441
T++ ++P
Sbjct: 290 TKQKSMP 296
>At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 822
Score = 28.7 bits (61), Expect = 4.2
Identities = 17/54 (31%), Positives = 23/54 (42%)
Frame = +1
Query: 325 EEISVKTADGYIVVEGKHEEKKDQHGYISRQFTRRYALPEGCTAESVESRLSSD 486
+EI D Y EG EE+ ++ R + L E C E+VES D
Sbjct: 48 DEIDSAYGDFYEYEEGVPEEESRKNNRYERVVNYEFELAEDCEDENVESEDDDD 101
>At2g27980.1 68415.m03391 expressed protein
Length = 1037
Score = 27.5 bits (58), Expect = 9.8
Identities = 16/48 (33%), Positives = 25/48 (52%)
Frame = +1
Query: 229 ASPCCRGSSLGSSIKSDKDKFQVNLDVQHFAPEEISVKTADGYIVVEG 372
A PC R + S +K + K +VN D H PE+ ++ + D + V G
Sbjct: 203 AKPCKRLTR--SMLKVEGIKSEVNADDDHVNPEKDAIGSEDNCVDVSG 248
>At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containing
protein low similarity to fertility restorer [Petunia x
hybrida] GI:22128587, post-transcriptional control of
chloroplast gene expression CRP1 [Zea mays] GI:3289002;
contains Pfam profile PF01535: PPR repeat
Length = 991
Score = 27.5 bits (58), Expect = 9.8
Identities = 15/48 (31%), Positives = 20/48 (41%)
Frame = -1
Query: 490 HHLKTAGTPQIQPYSPPAKRSDE*IDAICNHADLSSPRVCLQPRCSRP 347
HHL + ++ P S P S + H SS PRCS+P
Sbjct: 26 HHLALSSKARVFPLSLPCNFSSRVSFKLQLHCAASSSSSVSPPRCSKP 73
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,643,242
Number of Sequences: 28952
Number of extensions: 341334
Number of successful extensions: 900
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 872
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 900
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1614253080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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