Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00501
         (731 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces ...    70   4e-13
SPAC4C5.04 |rad31|uba4|SUMO E1-like activator enzyme Rad31|Schiz...    47   3e-06
SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme ...    37   0.003

>SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 500

 Score = 69.7 bits (163), Expect = 4e-13
 Identities = 29/89 (32%), Positives = 56/89 (62%)
 Frame = +1

Query: 247 IEMIVLPGIGAITIVDDCTVSDEDIGSNFFLEMSSKGLNRGSEALRLVLELNPAVQGHAV 426
           ++ ++LPGIG+  +VDD +V     G NFF++   +G +R      L+ +LNP V+   +
Sbjct: 43  LKNLILPGIGSFAVVDDTSVDFSMDGMNFFIQYDQEGKSRARCTASLLQQLNPNVEMEYL 102

Query: 427 QEPPDQILDENPDFFKSFSLVIATTISEK 513
           +  P+ ++D+N ++F  FS+V+++ + EK
Sbjct: 103 EMSPEALIDKNIEYFSKFSVVLSSNLKEK 131



 Score = 49.2 bits (112), Expect = 6e-07
 Identities = 19/37 (51%), Positives = 27/37 (72%)
 Frame = +2

Query: 143 KQYDRQLRLWGDHGQSALESGHICLINATALGTEILK 253
           ++YDRQ+RLW   GQ+A+E  H+CL+ A  +G E LK
Sbjct: 8   QKYDRQVRLWKAEGQNAIEKSHVCLLYANTVGCEALK 44


>SPAC4C5.04 |rad31|uba4|SUMO E1-like activator enzyme
           Rad31|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 307

 Score = 46.8 bits (106), Expect = 3e-06
 Identities = 27/83 (32%), Positives = 44/83 (53%)
 Frame = +1

Query: 256 IVLPGIGAITIVDDCTVSDEDIGSNFFLEMSSKGLNRGSEALRLVLELNPAVQGHAVQEP 435
           +VL GIG + ++D  TV ++D+   FF+E S  G  R +   + + ELNP V+   +   
Sbjct: 50  LVLSGIGKLCVLDSMTVYEKDVEEQFFIEASDIGQLRANVFKKKLHELNPLVE---IDTD 106

Query: 436 PDQILDENPDFFKSFSLVIATTI 504
              I + +      FS+VIAT +
Sbjct: 107 TSLISEIDEGKISKFSMVIATQL 129



 Score = 41.9 bits (94), Expect = 9e-05
 Identities = 18/35 (51%), Positives = 23/35 (65%)
 Frame = +2

Query: 149 YDRQLRLWGDHGQSALESGHICLINATALGTEILK 253
           YDRQ+RLWG + Q AL+   + LI A+ L  EI K
Sbjct: 14  YDRQIRLWGFNAQQALKQSRVLLITASPLANEIAK 48


>SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1012

 Score = 37.1 bits (82), Expect = 0.003
 Identities = 26/96 (27%), Positives = 44/96 (45%), Gaps = 1/96 (1%)
 Frame = +1

Query: 223 CYCLGY*NIEMIVLPGIGAITIVDDCTVSDEDIGSNFFLEMSSKGLNRGSEALRLVLELN 402
           C  LG    + + L G+ ++T+ D      ED+ S +FL     G+ R    +  + ELN
Sbjct: 45  CKGLGVEIAKNVCLAGVKSVTLYDPQPTRIEDLSSQYFLTEDDIGVPRAKVTVSKLAELN 104

Query: 403 PAVQGHAVQEPPDQILDE-NPDFFKSFSLVIATTIS 507
                   Q  P  ++DE + ++ K+F  V+ T  S
Sbjct: 105 --------QYVPVSVVDELSTEYLKNFKCVVVTETS 132



 Score = 27.9 bits (59), Expect = 1.6
 Identities = 13/36 (36%), Positives = 21/36 (58%)
 Frame = +2

Query: 146 QYDRQLRLWGDHGQSALESGHICLINATALGTEILK 253
           +YD Q+ ++G   Q  + S    L+ A A+G E+LK
Sbjct: 409 RYDGQIAVFGSEFQEKIASLSTFLVGAGAIGCEMLK 444


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,749,947
Number of Sequences: 5004
Number of extensions: 51841
Number of successful extensions: 106
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 100
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 106
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 345237368
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)