Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00501
(731 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g32410.1 68415.m03960 auxin-resistance protein, putative stro... 74 9e-14
At1g05180.1 68414.m00522 auxin-resistance protein AXR1 (AXR1) id... 74 1e-13
At1g05180.2 68414.m00521 auxin-resistance protein AXR1 (AXR1) id... 44 1e-04
At5g50680.1 68418.m06280 SUMO activating enzyme 1b (SAE1b) ident... 36 0.028
At5g50580.2 68418.m06266 SUMO activating enzyme, putative nearly... 36 0.028
At5g50580.1 68418.m06265 SUMO activating enzyme, putative nearly... 36 0.028
At5g06460.1 68418.m00724 ubiquitin activating enzyme 2 (UBA2) E1... 35 0.064
At2g30110.1 68415.m03664 ubiquitin activating enzyme 1 (UBA1) E1... 35 0.064
At4g24940.1 68417.m03572 SUMO activating enzyme 1a (SAE1a) ident... 32 0.45
At3g53980.2 68416.m05965 protease inhibitor/seed storage/lipid t... 31 1.0
At3g53980.1 68416.m05964 protease inhibitor/seed storage/lipid t... 31 1.0
At1g77240.1 68414.m08996 AMP-binding protein, putative strong si... 30 1.8
At5g44960.1 68418.m05514 F-box family protein contains F-box dom... 29 2.4
At1g75960.1 68414.m08822 AMP-binding protein, putative similar t... 29 4.2
At5g05960.1 68418.m00659 protease inhibitor/seed storage/lipid t... 28 5.6
At1g30890.1 68414.m03779 integral membrane HRF1 family protein c... 28 7.3
At1g21530.1 68414.m02693 AMP-binding protein, putative strong si... 28 7.3
At1g10410.1 68414.m01173 expressed protein similar to ESTs gb|N9... 28 7.3
At1g59650.1 68414.m06709 expressed protein ; supporting cDNA gi|... 27 9.7
At1g21540.1 68414.m02694 AMP-binding protein, putative strong si... 27 9.7
At1g09090.2 68414.m01015 respiratory burst oxidase protein B (Rb... 27 9.7
At1g09090.1 68414.m01014 respiratory burst oxidase protein B (Rb... 27 9.7
>At2g32410.1 68415.m03960 auxin-resistance protein, putative strong
similarity to SP|P42744 Auxin-resistance protein AXR1
{Arabidopsis thaliana}; contains Pfam profile PF00899:
ThiF family
Length = 523
Score = 74.1 bits (174), Expect = 9e-14
Identities = 35/88 (39%), Positives = 55/88 (62%)
Frame = +1
Query: 247 IEMIVLPGIGAITIVDDCTVSDEDIGSNFFLEMSSKGLNRGSEALRLVLELNPAVQGHAV 426
++ +V+ GIG+ITIVD V D+G+NF ++ S G +R + ELN +V+ + V
Sbjct: 41 LKNLVIGGIGSITIVDGSKVEIGDLGNNFMVDAKSVGQSRAKTVCGFLQELNDSVKANFV 100
Query: 427 QEPPDQILDENPDFFKSFSLVIATTISE 510
+E PD ++ +P FF F+LVIAT + E
Sbjct: 101 EENPDTLISTDPSFFSQFTLVIATQLVE 128
Score = 52.4 bits (120), Expect = 3e-07
Identities = 22/36 (61%), Positives = 28/36 (77%)
Frame = +2
Query: 146 QYDRQLRLWGDHGQSALESGHICLINATALGTEILK 253
+YDRQLR+WG+ GQSALE+ ICL+N G+E LK
Sbjct: 7 KYDRQLRIWGELGQSALETASICLLNCGPTGSEALK 42
Score = 45.2 bits (102), Expect = 5e-05
Identities = 19/45 (42%), Positives = 27/45 (60%)
Frame = +3
Query: 543 DINVPFILCQSVGFLGSFRIQIREHAVTETHPDNEQTDLRLDEPF 677
+ NV +L +S G G RI ++EH ET PD+ DLRL+ P+
Sbjct: 140 EANVMLVLARSYGLTGFVRISVKEHTAIETKPDHSLDDLRLNSPW 184
>At1g05180.1 68414.m00522 auxin-resistance protein AXR1 (AXR1)
identical to SP|P42744 Auxin-resistance protein AXR1
{Arabidopsis thaliana}; contains Pfam profile PF00899:
ThiF family; identical to cDNA ubiquitin activating
enzyme E1-related protein (AXR1) GI:304103
Length = 540
Score = 73.7 bits (173), Expect = 1e-13
Identities = 33/88 (37%), Positives = 53/88 (60%)
Frame = +1
Query: 247 IEMIVLPGIGAITIVDDCTVSDEDIGSNFFLEMSSKGLNRGSEALRLVLELNPAVQGHAV 426
++ +VL G+G+IT+VD V D+G+NF ++ S G ++ + ELN +V +
Sbjct: 57 LKNLVLGGVGSITVVDGSKVQFGDLGNNFMVDAKSVGQSKAKSVCAFLQELNDSVNAKFI 116
Query: 427 QEPPDQILDENPDFFKSFSLVIATTISE 510
+E PD ++ NP FF F+LVIAT + E
Sbjct: 117 EENPDTLITTNPSFFSQFTLVIATQLVE 144
Score = 50.8 bits (116), Expect = 9e-07
Identities = 21/36 (58%), Positives = 27/36 (75%)
Frame = +2
Query: 146 QYDRQLRLWGDHGQSALESGHICLINATALGTEILK 253
+YDRQLR+WG+ GQ+ALE ICL+N G+E LK
Sbjct: 23 KYDRQLRIWGEVGQAALEEASICLLNCGPTGSEALK 58
Score = 43.6 bits (98), Expect = 1e-04
Identities = 18/45 (40%), Positives = 28/45 (62%)
Frame = +3
Query: 543 DINVPFILCQSVGFLGSFRIQIREHAVTETHPDNEQTDLRLDEPF 677
D NV +L +S G G RI ++EH + ++ PD+ DLRL+ P+
Sbjct: 156 DANVKLVLVRSYGLAGFVRISVKEHPIIDSKPDHFLDDLRLNNPW 200
>At1g05180.2 68414.m00521 auxin-resistance protein AXR1 (AXR1)
identical to SP|P42744 Auxin-resistance protein AXR1
{Arabidopsis thaliana}; contains Pfam profile PF00899:
ThiF family; identical to cDNA ubiquitin activating
enzyme E1-related protein (AXR1) GI:304103
Length = 436
Score = 43.6 bits (98), Expect = 1e-04
Identities = 18/45 (40%), Positives = 28/45 (62%)
Frame = +3
Query: 543 DINVPFILCQSVGFLGSFRIQIREHAVTETHPDNEQTDLRLDEPF 677
D NV +L +S G G RI ++EH + ++ PD+ DLRL+ P+
Sbjct: 52 DANVKLVLVRSYGLAGFVRISVKEHPIIDSKPDHFLDDLRLNNPW 96
>At5g50680.1 68418.m06280 SUMO activating enzyme 1b (SAE1b)
identical to SUMO activating enzyme 1b [Arabidopsis
thaliana] GI:22652852; nearly identical to At5g50580;
contains Pfam profile PF00899: ThiF family
Length = 320
Score = 35.9 bits (79), Expect = 0.028
Identities = 28/98 (28%), Positives = 44/98 (44%), Gaps = 3/98 (3%)
Frame = +1
Query: 256 IVLPGIGAITIVDDCTVSDEDIGSNFFL---EMSSKGLNRGSEALRLVLELNPAVQGHAV 426
IVL G+G++T++DD V+ E +NF + E + G + + NP V H
Sbjct: 50 IVLAGVGSVTLLDDRLVTTEVFNANFLILPDENAYVGKTVAEICCDSLKDFNPMV--HVS 107
Query: 427 QEPPDQILDENPDFFKSFSLVIATTISEK*RKVYLNTC 540
E D + DFF+ F +V+ S +K C
Sbjct: 108 IEKGD-LSTLGVDFFEKFDVVVIGYSSRATKKAVNEKC 144
Score = 29.5 bits (63), Expect = 2.4
Identities = 12/35 (34%), Positives = 16/35 (45%)
Frame = +2
Query: 149 YDRQLRLWGDHGQSALESGHICLINATALGTEILK 253
YDRQ+R+WG Q L H+ + E K
Sbjct: 14 YDRQIRVWGAGAQRRLSKSHVLVSGIKGTVAEFCK 48
>At5g50580.2 68418.m06266 SUMO activating enzyme, putative nearly
identical to SUMO activating enzyme 1b [Arabidopsis
thaliana] GI:22652852; nearly identical to At5g50680;
contains Pfam profile PF00899: ThiF family
Length = 320
Score = 35.9 bits (79), Expect = 0.028
Identities = 28/98 (28%), Positives = 44/98 (44%), Gaps = 3/98 (3%)
Frame = +1
Query: 256 IVLPGIGAITIVDDCTVSDEDIGSNFFL---EMSSKGLNRGSEALRLVLELNPAVQGHAV 426
IVL G+G++T++DD V+ E +NF + E + G + + NP V H
Sbjct: 50 IVLAGVGSVTLLDDRLVTTEVFNANFLILPDENAYVGKTVAEICCDSLKDFNPMV--HVS 107
Query: 427 QEPPDQILDENPDFFKSFSLVIATTISEK*RKVYLNTC 540
E D + DFF+ F +V+ S +K C
Sbjct: 108 IEKGD-LSTLGVDFFEKFDVVVIGYSSRATKKAVNEKC 144
Score = 29.5 bits (63), Expect = 2.4
Identities = 12/35 (34%), Positives = 16/35 (45%)
Frame = +2
Query: 149 YDRQLRLWGDHGQSALESGHICLINATALGTEILK 253
YDRQ+R+WG Q L H+ + E K
Sbjct: 14 YDRQIRVWGAGAQRRLSKSHVLVSGIKGTVAEFCK 48
>At5g50580.1 68418.m06265 SUMO activating enzyme, putative nearly
identical to SUMO activating enzyme 1b [Arabidopsis
thaliana] GI:22652852; nearly identical to At5g50680;
contains Pfam profile PF00899: ThiF family
Length = 318
Score = 35.9 bits (79), Expect = 0.028
Identities = 28/98 (28%), Positives = 44/98 (44%), Gaps = 3/98 (3%)
Frame = +1
Query: 256 IVLPGIGAITIVDDCTVSDEDIGSNFFL---EMSSKGLNRGSEALRLVLELNPAVQGHAV 426
IVL G+G++T++DD V+ E +NF + E + G + + NP V H
Sbjct: 50 IVLAGVGSVTLLDDRLVTTEVFNANFLILPDENAYVGKTVAEICCDSLKDFNPMV--HVS 107
Query: 427 QEPPDQILDENPDFFKSFSLVIATTISEK*RKVYLNTC 540
E D + DFF+ F +V+ S +K C
Sbjct: 108 IEKGD-LSTLGVDFFEKFDVVVIGYSSRATKKAVNEKC 144
Score = 29.5 bits (63), Expect = 2.4
Identities = 12/35 (34%), Positives = 16/35 (45%)
Frame = +2
Query: 149 YDRQLRLWGDHGQSALESGHICLINATALGTEILK 253
YDRQ+R+WG Q L H+ + E K
Sbjct: 14 YDRQIRVWGAGAQRRLSKSHVLVSGIKGTVAEFCK 48
>At5g06460.1 68418.m00724 ubiquitin activating enzyme 2 (UBA2) E1;
identical to gi:1703477
Length = 1077
Score = 34.7 bits (76), Expect = 0.064
Identities = 18/52 (34%), Positives = 28/52 (53%)
Frame = +1
Query: 256 IVLPGIGAITIVDDCTVSDEDIGSNFFLEMSSKGLNRGSEALRLVLELNPAV 411
I+L G+ ++T+ D+ V D+ SNF G NR ++ + ELN AV
Sbjct: 111 IILAGVKSVTLHDENVVELWDLSSNFVFTEEDIGKNRALASVHKLQELNNAV 162
Score = 33.9 bits (74), Expect = 0.11
Identities = 15/38 (39%), Positives = 22/38 (57%)
Frame = +2
Query: 140 NKQYDRQLRLWGDHGQSALESGHICLINATALGTEILK 253
N +YD Q+ ++G Q LE + ++ A ALG E LK
Sbjct: 469 NSRYDAQISVFGSTLQKKLEDARVFVVGAGALGCEFLK 506
>At2g30110.1 68415.m03664 ubiquitin activating enzyme 1 (UBA1) E1;
identical to GB:U80808
Length = 1080
Score = 34.7 bits (76), Expect = 0.064
Identities = 16/52 (30%), Positives = 29/52 (55%)
Frame = +1
Query: 256 IVLPGIGAITIVDDCTVSDEDIGSNFFLEMSSKGLNRGSEALRLVLELNPAV 411
++L G+ ++T+ D+ V D+ SNF G NR +++ + +LN AV
Sbjct: 114 LILAGVKSVTLHDERVVELWDLSSNFVFSEDDVGKNRADASVQKLQDLNNAV 165
Score = 31.5 bits (68), Expect = 0.60
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +2
Query: 140 NKQYDRQLRLWGDHGQSALESGHICLINATALGTEILK 253
N +YD Q+ ++G Q LE + + + ALG E LK
Sbjct: 472 NSRYDAQISVFGAKFQKKLEDAKVFTVGSGALGCEFLK 509
>At4g24940.1 68417.m03572 SUMO activating enzyme 1a (SAE1a)
identical to SUMO activating enzyme 1a [Arabidopsis
thaliana] GI:22652850; contains Pfam profile PF00899:
ThiF family
Length = 322
Score = 31.9 bits (69), Expect = 0.45
Identities = 23/82 (28%), Positives = 42/82 (51%), Gaps = 3/82 (3%)
Frame = +1
Query: 256 IVLPGIGAITIVDDCTVSDEDIGSNFFL---EMSSKGLNRGSEALRLVLELNPAVQGHAV 426
IVL G+G++T++DD + E + +NF + E G + + NP V+ +V
Sbjct: 50 IVLAGVGSVTLMDDRLANMEALNANFLIPPDENVYSGKTVAEICSDSLKDFNPMVR-VSV 108
Query: 427 QEPPDQILDENPDFFKSFSLVI 492
++ +L DFF+ F +V+
Sbjct: 109 EKGDLSML--GTDFFEQFDVVV 128
Score = 30.7 bits (66), Expect = 1.0
Identities = 13/35 (37%), Positives = 17/35 (48%)
Frame = +2
Query: 149 YDRQLRLWGDHGQSALESGHICLINATALGTEILK 253
YDRQ+R+WG + Q L HI + E K
Sbjct: 14 YDRQIRVWGANAQRRLTKAHILVSGIKGTVAEFCK 48
>At3g53980.2 68416.m05965 protease inhibitor/seed storage/lipid
transfer protein (LTP) family protein contains Pfam
protease inhibitor/seed storage/LTP family domain
PF00234
Length = 114
Score = 30.7 bits (66), Expect = 1.0
Identities = 11/22 (50%), Positives = 13/22 (59%)
Frame = +2
Query: 506 VKSNAKFISTPVGYKCSFYTLP 571
+ F + PVGYKC YTLP
Sbjct: 93 IPKRCNFANRPVGYKCGAYTLP 114
>At3g53980.1 68416.m05964 protease inhibitor/seed storage/lipid
transfer protein (LTP) family protein contains Pfam
protease inhibitor/seed storage/LTP family domain
PF00234
Length = 114
Score = 30.7 bits (66), Expect = 1.0
Identities = 11/22 (50%), Positives = 13/22 (59%)
Frame = +2
Query: 506 VKSNAKFISTPVGYKCSFYTLP 571
+ F + PVGYKC YTLP
Sbjct: 93 IPKRCNFANRPVGYKCGAYTLP 114
>At1g77240.1 68414.m08996 AMP-binding protein, putative strong
similarity to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501
Length = 545
Score = 29.9 bits (64), Expect = 1.8
Identities = 17/41 (41%), Positives = 21/41 (51%)
Frame = +1
Query: 355 GLNRGSEALRLVLELNPAVQGHAVQEPPDQILDENPDFFKS 477
G N S L VL NPA++ AV PD++ E P F S
Sbjct: 448 GENISSTELEAVLYTNPAIKEAAVVAKPDKMWGETPCAFVS 488
>At5g44960.1 68418.m05514 F-box family protein contains F-box domain
Pfam:PF00646
Length = 416
Score = 29.5 bits (63), Expect = 2.4
Identities = 17/60 (28%), Positives = 31/60 (51%)
Frame = +1
Query: 424 VQEPPDQILDENPDFFKSFSLVIATTISEK*RKVYLNTCGI*MFLLYFANQLDS*GLLEF 603
+ E PD +L + ++ V + S++ R ++LN G+ +F L F N GL++F
Sbjct: 7 INELPDSLLTQILLDLRTKDSVKTSVSSKRWRNLWLNVPGLDLFSLQFTNPHHEEGLIKF 66
>At1g75960.1 68414.m08822 AMP-binding protein, putative similar to
AMP-binding protein GI:1903034 from [Brassica napus];
contains Pfam profile: PF00501 AMP-binding enzyme;
identical to cDNA adenosine monophosphate binding
protein 8 AMPBP8 (AMPBP8) GI:20799724
Length = 544
Score = 28.7 bits (61), Expect = 4.2
Identities = 18/43 (41%), Positives = 20/43 (46%)
Frame = +1
Query: 349 SKGLNRGSEALRLVLELNPAVQGHAVQEPPDQILDENPDFFKS 477
S G N S + VL NPAV AV PD+ E P F S
Sbjct: 445 SGGENVSSVEVEAVLYTNPAVNEAAVVARPDEFWGETPCAFVS 487
>At5g05960.1 68418.m00659 protease inhibitor/seed storage/lipid
transfer protein (LTP) family protein contains Pfam
protease inhibitor/seed storage/LTP family domain
PF00234
Length = 116
Score = 28.3 bits (60), Expect = 5.6
Identities = 10/22 (45%), Positives = 12/22 (54%)
Frame = +2
Query: 506 VKSNAKFISTPVGYKCSFYTLP 571
+ + PVGYKC YTLP
Sbjct: 95 IPKRCNIANRPVGYKCGAYTLP 116
>At1g30890.1 68414.m03779 integral membrane HRF1 family protein
contains Pfam domain PF03878: Hrf1 family
Length = 269
Score = 27.9 bits (59), Expect = 7.3
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = -3
Query: 462 WVLIKYLVGWFLDCMTL 412
W+ +K LVGWFL M L
Sbjct: 149 WLFVKGLVGWFLQVMLL 165
>At1g21530.1 68414.m02693 AMP-binding protein, putative strong
similarity to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501; identical to cDNA adenosine monophosphate
binding protein 10 AMPBP10 (AMPBP10) GI:20799728
Length = 547
Score = 27.9 bits (59), Expect = 7.3
Identities = 16/41 (39%), Positives = 20/41 (48%)
Frame = +1
Query: 355 GLNRGSEALRLVLELNPAVQGHAVQEPPDQILDENPDFFKS 477
G N S + VL NP V+ AV PD++ E P F S
Sbjct: 445 GENISSAEIETVLYTNPVVKEAAVVAKPDKMWGETPCAFVS 485
>At1g10410.1 68414.m01173 expressed protein similar to ESTs
gb|N96021 and gb|N96863
Length = 485
Score = 27.9 bits (59), Expect = 7.3
Identities = 16/46 (34%), Positives = 27/46 (58%), Gaps = 1/46 (2%)
Frame = +1
Query: 316 DIGSNFFLEMSSKGLNRGSEALRL-VLELNPAVQGHAVQEPPDQIL 450
DI + F +S KG + L++ VL++ +QG+ +E P+QIL
Sbjct: 419 DIDMHRFGYISRKGFETFIDRLKICVLDVGLTIQGNKPEELPEQIL 464
>At1g59650.1 68414.m06709 expressed protein ; supporting cDNA
gi|6520232|dbj|AB028233.1|
Length = 492
Score = 27.5 bits (58), Expect = 9.7
Identities = 16/46 (34%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +1
Query: 316 DIGSNFFLEMSSKGLNRGSEALR-LVLELNPAVQGHAVQEPPDQIL 450
DI + F +S KG + L+ VL++ +QG+ +E P+QIL
Sbjct: 424 DIDMHRFSYISRKGFEAFLDRLKNCVLDVGLTIQGNKPEELPEQIL 469
>At1g21540.1 68414.m02694 AMP-binding protein, putative strong
similarity to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501; identical to cDNA adenosine monophosphate
binding protein 9 AMPBP9 (AMPBP9) GI:20799726
Length = 550
Score = 27.5 bits (58), Expect = 9.7
Identities = 16/41 (39%), Positives = 20/41 (48%)
Frame = +1
Query: 355 GLNRGSEALRLVLELNPAVQGHAVQEPPDQILDENPDFFKS 477
G N S + VL NP V+ AV PD++ E P F S
Sbjct: 450 GENISSAEVETVLYTNPVVKEAAVVAKPDKMWGETPCAFVS 490
>At1g09090.2 68414.m01015 respiratory burst oxidase protein B
(RbohB) / NADPH oxidase identical to respiratory burst
oxidase protein B from Arabidopsis thaliana [gi:3242783]
Length = 843
Score = 27.5 bits (58), Expect = 9.7
Identities = 18/57 (31%), Positives = 27/57 (47%)
Frame = -3
Query: 573 IGKV*KEHLYPTGVEINFALLFTYCSSYH*TE*FKKVWVLIKYLVGWFLDCMTLYCW 403
+ K+ + L PT + N F SY E +K++WVL W C+TL+ W
Sbjct: 263 LNKMLSQKLIPTK-DRNPVKRFAMNISYFFLENWKRIWVLTL----WISICITLFTW 314
>At1g09090.1 68414.m01014 respiratory burst oxidase protein B
(RbohB) / NADPH oxidase identical to respiratory burst
oxidase protein B from Arabidopsis thaliana [gi:3242783]
Length = 622
Score = 27.5 bits (58), Expect = 9.7
Identities = 18/57 (31%), Positives = 27/57 (47%)
Frame = -3
Query: 573 IGKV*KEHLYPTGVEINFALLFTYCSSYH*TE*FKKVWVLIKYLVGWFLDCMTLYCW 403
+ K+ + L PT + N F SY E +K++WVL W C+TL+ W
Sbjct: 263 LNKMLSQKLIPTK-DRNPVKRFAMNISYFFLENWKRIWVLTL----WISICITLFTW 314
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,892,354
Number of Sequences: 28952
Number of extensions: 256838
Number of successful extensions: 563
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 544
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 563
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1604469728
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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