Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00500
(733 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 145 5e-36
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 145 5e-36
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 145 5e-36
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 42 7e-05
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 40 3e-04
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 34 0.018
SPBC21.01 |mis17|SPBC776.19|kinetochore protein Mis17|Schizosacc... 28 1.2
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 3.6
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 27 3.6
SPBC336.15 |pic1|SPBC685.01|INCENP-like|Schizosaccharomyces pomb... 26 6.4
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 26 6.4
SPAC23D3.08 |usp108||U1 snRNP-associated protein Usp108|Schizosa... 25 8.4
SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomy... 25 8.4
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 8.4
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 145 bits (352), Expect = 5e-36
Identities = 65/85 (76%), Positives = 74/85 (87%)
Frame = +1
Query: 253 QVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYV 432
+VETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V
Sbjct: 264 RVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNV 323
Query: 433 AGDSKNNPPKGAADFTAQVIVLNHP 507
GDSKN+PP G A FTAQVI+LNHP
Sbjct: 324 CGDSKNDPPMGCASFTAQVIILNHP 348
Score = 135 bits (326), Expect = 7e-33
Identities = 58/87 (66%), Positives = 71/87 (81%)
Frame = +2
Query: 2 GTSPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPARP 181
G +P V FVP+SG+ GDNM+EP+T MPW++GWQ E K G GK L+EA+D+I PPARP
Sbjct: 180 GFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARP 239
Query: 182 TXKXLRLPLQDVYKIGGIGTVPVGKLK 262
T K LRLPLQDVYKIGGIGTVPVG+++
Sbjct: 240 TDKPLRLPLQDVYKIGGIGTVPVGRVE 266
Score = 107 bits (258), Expect = 1e-24
Identities = 51/95 (53%), Positives = 63/95 (66%)
Frame = +3
Query: 432 CW*LQKQPT*GCCRFYSSSHCA*PSCQISNGYTPVLDCXTAHIACKFAEIKEKVDRRTGK 611
C + P GC F + QIS GY+PVLDC TAHIACKFAE+ EK+DRR+GK
Sbjct: 324 CGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGK 383
Query: 612 STEVNPKSIKSGDAAIVNLVPSKPLCVESFQNSHP 716
E +PK +KSGDA I +VPSKP+CVE+F + P
Sbjct: 384 KIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAP 418
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 145 bits (352), Expect = 5e-36
Identities = 65/85 (76%), Positives = 74/85 (87%)
Frame = +1
Query: 253 QVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYV 432
+VETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V
Sbjct: 264 RVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNV 323
Query: 433 AGDSKNNPPKGAADFTAQVIVLNHP 507
GDSKN+PP G A FTAQVI+LNHP
Sbjct: 324 CGDSKNDPPMGCASFTAQVIILNHP 348
Score = 135 bits (326), Expect = 7e-33
Identities = 58/87 (66%), Positives = 71/87 (81%)
Frame = +2
Query: 2 GTSPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPARP 181
G +P V FVP+SG+ GDNM+EP+T MPW++GWQ E K G GK L+EA+D+I PPARP
Sbjct: 180 GFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARP 239
Query: 182 TXKXLRLPLQDVYKIGGIGTVPVGKLK 262
T K LRLPLQDVYKIGGIGTVPVG+++
Sbjct: 240 TDKPLRLPLQDVYKIGGIGTVPVGRVE 266
Score = 107 bits (258), Expect = 1e-24
Identities = 51/95 (53%), Positives = 63/95 (66%)
Frame = +3
Query: 432 CW*LQKQPT*GCCRFYSSSHCA*PSCQISNGYTPVLDCXTAHIACKFAEIKEKVDRRTGK 611
C + P GC F + QIS GY+PVLDC TAHIACKFAE+ EK+DRR+GK
Sbjct: 324 CGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGK 383
Query: 612 STEVNPKSIKSGDAAIVNLVPSKPLCVESFQNSHP 716
E +PK +KSGDA I +VPSKP+CVE+F + P
Sbjct: 384 KIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAP 418
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 145 bits (352), Expect = 5e-36
Identities = 65/85 (76%), Positives = 74/85 (87%)
Frame = +1
Query: 253 QVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYV 432
+VETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V
Sbjct: 264 RVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNV 323
Query: 433 AGDSKNNPPKGAADFTAQVIVLNHP 507
GDSKN+PP G A FTAQVI+LNHP
Sbjct: 324 CGDSKNDPPMGCASFTAQVIILNHP 348
Score = 135 bits (326), Expect = 7e-33
Identities = 58/87 (66%), Positives = 71/87 (81%)
Frame = +2
Query: 2 GTSPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPARP 181
G +P V FVP+SG+ GDNM+EP+T MPW++GWQ E K G GK L+EA+D+I PPARP
Sbjct: 180 GFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARP 239
Query: 182 TXKXLRLPLQDVYKIGGIGTVPVGKLK 262
T K LRLPLQDVYKIGGIGTVPVG+++
Sbjct: 240 TDKPLRLPLQDVYKIGGIGTVPVGRVE 266
Score = 107 bits (258), Expect = 1e-24
Identities = 51/95 (53%), Positives = 63/95 (66%)
Frame = +3
Query: 432 CW*LQKQPT*GCCRFYSSSHCA*PSCQISNGYTPVLDCXTAHIACKFAEIKEKVDRRTGK 611
C + P GC F + QIS GY+PVLDC TAHIACKFAE+ EK+DRR+GK
Sbjct: 324 CGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGK 383
Query: 612 STEVNPKSIKSGDAAIVNLVPSKPLCVESFQNSHP 716
E +PK +KSGDA I +VPSKP+CVE+F + P
Sbjct: 384 KIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAP 418
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 42.3 bits (95), Expect = 7e-05
Identities = 26/76 (34%), Positives = 38/76 (50%), Gaps = 2/76 (2%)
Frame = +2
Query: 2 GTSPAAVAFVPISGWHGDNMLEPSTK--MPWFKGWQVERKEGKADGKCLIEALDAILPPA 175
G + V FVPIS G N+++ + W+KG L+ ALD ++PP
Sbjct: 351 GFKTSNVHFVPISAISGTNLIQKDSSDLYKWYKG------------PTLLSALDQLVPPE 398
Query: 176 RPTXKXLRLPLQDVYK 223
+P K LRL + DVY+
Sbjct: 399 KPYRKPLRLSIDDVYR 414
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 40.3 bits (90), Expect = 3e-04
Identities = 21/62 (33%), Positives = 36/62 (58%), Gaps = 2/62 (3%)
Frame = +1
Query: 253 QVETGVLKPGTIV--VFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRG 426
+VE G LK G + V +++ T V +EM + L AV GDN G ++++ ++L+RG
Sbjct: 273 RVERGTLKKGAEIEIVGYGSHLKTTVTGIEMFKKQLDAAVAGDNCGLLLRSIKREQLKRG 332
Query: 427 YV 432
+
Sbjct: 333 MI 334
Score = 31.5 bits (68), Expect = 0.13
Identities = 15/42 (35%), Positives = 26/42 (61%), Gaps = 1/42 (2%)
Frame = +2
Query: 140 LIEALDAILP-PARPTXKXLRLPLQDVYKIGGIGTVPVGKLK 262
L+EA+D+ + P R T + ++DV+ I G GTV G+++
Sbjct: 234 LMEAVDSYITLPERKTDVPFLMAIEDVFSISGRGTVVTGRVE 275
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 34.3 bits (75), Expect = 0.018
Identities = 18/65 (27%), Positives = 33/65 (50%)
Frame = +3
Query: 513 ISNGYTPVLDCXTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCV 692
++ GY+ V+ TA FA++ K+D +T + ++ P G I L P+C+
Sbjct: 573 LTTGYSCVMHIHTAVEEVSFAKLLHKLD-KTNRKSKKPPMFATKGMKIIAELETQTPVCM 631
Query: 693 ESFQN 707
E F++
Sbjct: 632 ERFED 636
>SPBC21.01 |mis17|SPBC776.19|kinetochore protein
Mis17|Schizosaccharomyces pombe|chr 2|||Manual
Length = 441
Score = 28.3 bits (60), Expect = 1.2
Identities = 16/72 (22%), Positives = 32/72 (44%)
Frame = +1
Query: 199 SSPARRIQNRWYWYRARRQVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDN 378
S ++IQ + R +ET +I+ +P N + S++ ++ QE PG+
Sbjct: 133 SHKQKKIQRESIPFHKRDNIETSDAYSSSILENSPPNKVQRLSSLDSSQDSFQEEHPGNV 192
Query: 379 VGFNVKNVSVKE 414
G + + +E
Sbjct: 193 TGTTFSSQAPEE 204
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 3.6
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -2
Query: 417 QFLDGHVLYVETYIVSRYSFLESFV 343
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 227
Score = 26.6 bits (56), Expect = 3.6
Identities = 16/48 (33%), Positives = 19/48 (39%)
Frame = -3
Query: 359 SWRASWCISTDLTSVVMLAGAKTTMVPGFNTPVSTCRRARYQYHRFCI 216
SW SW +ST L +V + G T ST R RF I
Sbjct: 97 SWNPSWMVSTILVGLVSFMTSDEITTGGIVTSESTRRTYAKDTKRFNI 144
>SPBC336.15 |pic1|SPBC685.01|INCENP-like|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1018
Score = 25.8 bits (54), Expect = 6.4
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = +1
Query: 247 RRQVETGVLKPGTIVVFAPANITTEVKSVEMHHEAL 354
+R VE +LKP + +P IT+ KS + EAL
Sbjct: 344 KRSVENEMLKPHPTIFESPPEITSFDKSNAVEAEAL 379
>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1233
Score = 25.8 bits (54), Expect = 6.4
Identities = 10/39 (25%), Positives = 22/39 (56%)
Frame = -2
Query: 441 VTSNITTTQFLDGHVLYVETYIVSRYSFLESFVVHLHRF 325
+T ++ + L H+ +++ SR +FL ++H+H F
Sbjct: 1012 LTQSLQSFSHLSNHIEVLDSTRQSRLTFLCHLILHMHGF 1050
>SPAC23D3.08 |usp108||U1 snRNP-associated protein
Usp108|Schizosaccharomyces pombe|chr 1|||Manual
Length = 382
Score = 25.4 bits (53), Expect = 8.4
Identities = 25/74 (33%), Positives = 35/74 (47%), Gaps = 4/74 (5%)
Frame = -2
Query: 420 TQFLDGHVLYVET---YIVSRYSFLESFVVHLHRFDFSSDVGGGKDNNGTWFQH-TSFNL 253
T +D V+ +ET YI+SR SF+ V + D + + G + T F H +S
Sbjct: 162 TNEIDASVV-IETLLKYILSRISFIPRVSVVPYIPDAAYGI-YGSPSTFTQFNHMSSMVT 219
Query: 252 PTGTVPIPPILYTS 211
P IPPI TS
Sbjct: 220 PENPYGIPPIALTS 233
>SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1115
Score = 25.4 bits (53), Expect = 8.4
Identities = 10/34 (29%), Positives = 18/34 (52%)
Frame = -2
Query: 333 HRFDFSSDVGGGKDNNGTWFQHTSFNLPTGTVPI 232
H++DF D K ++G+ H + PT + P+
Sbjct: 59 HKYDFEIDRDSLKSDSGSPRLHQNATAPTSSTPL 92
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 25.4 bits (53), Expect = 8.4
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = -1
Query: 658 MAASPDLMDFGLTSVDLPVRRSTFS 584
++ +PDL D L+SVD P++ +T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,296,843
Number of Sequences: 5004
Number of extensions: 69885
Number of successful extensions: 194
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 181
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 194
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 345237368
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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