BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00491 (735 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 136 1e-32 At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 136 1e-32 At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 136 1e-32 At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 136 1e-32 At1g35550.1 68414.m04414 elongation factor Tu C-terminal domain-... 83 2e-16 At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 46 2e-05 At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 45 5e-05 At4g27900.2 68417.m04005 expressed protein 32 0.45 At4g27900.1 68417.m04004 expressed protein 32 0.45 At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 30 1.8 At1g55420.1 68414.m06339 DC1 domain-containing protein contains ... 29 4.2 At5g56440.1 68418.m07045 F-box family protein contains F-box dom... 28 7.4 At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 28 7.4 At1g55380.1 68414.m06334 DC1 domain-containing protein contains ... 28 7.4 >At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha identical to SWISS-PROT:P13905 elongation factor 1-alpha (EF-1-alpha) [Arabidopsis thaliana] Length = 449 Score = 136 bits (330), Expect = 1e-32 Identities = 59/81 (72%), Positives = 73/81 (90%) Frame = +2 Query: 11 EAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPV 190 EA+PGDNVGFNVKNV+VK+L+RGYVA +SK++P KGAA+FT+QVI++NHPGQI NGY PV Sbjct: 289 EALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPV 348 Query: 191 LDCXTAHIACKFAEIKEKVDR 253 LDC T+HIA KF+EI K+DR Sbjct: 349 LDCHTSHIAVKFSEILTKIDR 369 Score = 89.0 bits (211), Expect = 3e-18 Identities = 39/60 (65%), Positives = 50/60 (83%) Frame = +1 Query: 256 TGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVN 435 +GK E PK +K+GDA +V + P+KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V+ Sbjct: 371 SGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVD 430 >At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha identical to GB:CAA34456 from [Arabidopsis thaliana] (Plant Mol. Biol. 14 (1), 107-110 (1990)) Length = 449 Score = 136 bits (330), Expect = 1e-32 Identities = 59/81 (72%), Positives = 73/81 (90%) Frame = +2 Query: 11 EAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPV 190 EA+PGDNVGFNVKNV+VK+L+RGYVA +SK++P KGAA+FT+QVI++NHPGQI NGY PV Sbjct: 289 EALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPV 348 Query: 191 LDCXTAHIACKFAEIKEKVDR 253 LDC T+HIA KF+EI K+DR Sbjct: 349 LDCHTSHIAVKFSEILTKIDR 369 Score = 89.0 bits (211), Expect = 3e-18 Identities = 39/60 (65%), Positives = 50/60 (83%) Frame = +1 Query: 256 TGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVN 435 +GK E PK +K+GDA +V + P+KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V+ Sbjct: 371 SGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVD 430 >At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha identical to GB:CAA34456 from [Arabidopsis thaliana] (Plant Mol. Biol. 14 (1), 107-110 (1990)) Length = 449 Score = 136 bits (330), Expect = 1e-32 Identities = 59/81 (72%), Positives = 73/81 (90%) Frame = +2 Query: 11 EAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPV 190 EA+PGDNVGFNVKNV+VK+L+RGYVA +SK++P KGAA+FT+QVI++NHPGQI NGY PV Sbjct: 289 EALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPV 348 Query: 191 LDCXTAHIACKFAEIKEKVDR 253 LDC T+HIA KF+EI K+DR Sbjct: 349 LDCHTSHIAVKFSEILTKIDR 369 Score = 89.0 bits (211), Expect = 3e-18 Identities = 39/60 (65%), Positives = 50/60 (83%) Frame = +1 Query: 256 TGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVN 435 +GK E PK +K+GDA +V + P+KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V+ Sbjct: 371 SGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVD 430 >At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha identical to GB:CAA34456 from [Arabidopsis thaliana] (Plant Mol. Biol. 14 (1), 107-110 (1990)) Length = 449 Score = 136 bits (330), Expect = 1e-32 Identities = 59/81 (72%), Positives = 73/81 (90%) Frame = +2 Query: 11 EAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPV 190 EA+PGDNVGFNVKNV+VK+L+RGYVA +SK++P KGAA+FT+QVI++NHPGQI NGY PV Sbjct: 289 EALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPV 348 Query: 191 LDCXTAHIACKFAEIKEKVDR 253 LDC T+HIA KF+EI K+DR Sbjct: 349 LDCHTSHIAVKFSEILTKIDR 369 Score = 89.0 bits (211), Expect = 3e-18 Identities = 39/60 (65%), Positives = 50/60 (83%) Frame = +1 Query: 256 TGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVN 435 +GK E PK +K+GDA +V + P+KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V+ Sbjct: 371 SGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVD 430 >At1g35550.1 68414.m04414 elongation factor Tu C-terminal domain-containing protein similar to SP|P13905 Elongation factor 1-alpha (EF-1-alpha) {Arabidopsis thaliana}; contains Pfam profile PF03143: Elongation factor Tu C-terminal domain Length = 104 Score = 83.0 bits (196), Expect = 2e-16 Identities = 35/59 (59%), Positives = 47/59 (79%) Frame = +1 Query: 256 TGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 432 TG E PK +K+ +AAI+N+ P+KP+ VE++ +PPLGRFA+RDMRQTV VGVIK+V Sbjct: 37 TGHEIEKEPKFLKNSEAAIINMTPTKPMVVEAYSAYPPLGRFAIRDMRQTVGVGVIKSV 95 Score = 58.0 bits (134), Expect = 6e-09 Identities = 24/34 (70%), Positives = 28/34 (82%) Frame = +2 Query: 149 LNHPGQISNGYTPVLDCXTAHIACKFAEIKEKVD 250 +NH GQI NGYTPVLDC T+HIA KF+EI K+D Sbjct: 1 MNHLGQIKNGYTPVLDCHTSHIAVKFSEILTKID 34 >At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / EF-1-alpha, putative contains similarity to SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha) [Aeropyrum pernix] Length = 667 Score = 46.4 bits (105), Expect = 2e-05 Identities = 20/61 (32%), Positives = 37/61 (60%) Frame = +1 Query: 250 PSTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKA 429 P TG+ T+ +P+ + + +A++ + P+CVE+F E LGR +R +TVA+G + Sbjct: 603 PKTGQPTKKSPRCLTAKQSAMLEVSLQNPVCVETFSESRALGRVFLRSSGRTVAMGKVTR 662 Query: 430 V 432 + Sbjct: 663 I 663 >At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein, putative similar to EF-1-alpha-related GTP-binding protein gi|1009232|gb|AAA79032 Length = 532 Score = 45.2 bits (102), Expect = 5e-05 Identities = 17/45 (37%), Positives = 29/45 (64%) Frame = +1 Query: 289 IKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVI 423 +K+G A + + + +C+E F +FP LGRF +R +T+AVG + Sbjct: 478 VKNGAAVVCRIQVTNSICIEKFSDFPQLGRFTLRTEGKTIAVGKV 522 Score = 34.7 bits (76), Expect = 0.064 Identities = 20/83 (24%), Positives = 38/83 (45%), Gaps = 2/83 (2%) Frame = +2 Query: 8 REAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLN--HPGQISNGY 181 + A PG+N+ + + +++ G+V S NP +F AQ+ +L + GY Sbjct: 383 KRAGPGENLRVRITGIEDEDILSGFVL-SSIVNPVPAVTEFVAQLQILELLDNAIFTAGY 441 Query: 182 TPVLDCXTAHIACKFAEIKEKVD 250 +L C+ E+K ++D Sbjct: 442 KAILHIHAVVEECEIIELKSQID 464 >At4g27900.2 68417.m04005 expressed protein Length = 261 Score = 31.9 bits (69), Expect = 0.45 Identities = 11/31 (35%), Positives = 21/31 (67%) Frame = +1 Query: 280 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 372 PKS+ SG+ + +++V K ++ F +FPP+ Sbjct: 144 PKSVSSGNLSSMDMVEHKDAVIQGFPDFPPV 174 >At4g27900.1 68417.m04004 expressed protein Length = 261 Score = 31.9 bits (69), Expect = 0.45 Identities = 11/31 (35%), Positives = 21/31 (67%) Frame = +1 Query: 280 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 372 PKS+ SG+ + +++V K ++ F +FPP+ Sbjct: 144 PKSVSSGNLSSMDMVEHKDAVIQGFPDFPPV 174 >At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) identical to SWISS-PROT:P17745 elongation factor Tu, chloroplast precursor (EF-Tu) [Arabidopsis thaliana] Length = 476 Score = 29.9 bits (64), Expect = 1.8 Identities = 21/83 (25%), Positives = 34/83 (40%), Gaps = 3/83 (3%) Frame = +2 Query: 11 EAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQIS---NGY 181 EA+ GDNVG ++ + +++RG V + P + V+ G+ S GY Sbjct: 345 EALAGDNVGLLLRGIQKADIQRGMVLAKPGSITPHTKFEAIIYVLKKEEGGRHSPFFAGY 404 Query: 182 TPVLDCXTAHIACKFAEIKEKVD 250 P T + K +I D Sbjct: 405 RPQFYMRTTDVTGKVTKIMNDKD 427 >At1g55420.1 68414.m06339 DC1 domain-containing protein contains Pfam profile PF03107: DC1 domain Length = 725 Score = 28.7 bits (61), Expect = 4.2 Identities = 15/38 (39%), Positives = 21/38 (55%), Gaps = 5/38 (13%) Frame = +2 Query: 602 IFYKACN---VTLFYNLYKVIHNI--SETFCYDCKLKC 700 I+ K C+ V LFY ++ N S FCY C+L+C Sbjct: 619 IYLKPCHIFKVGLFYKEVEIARNDGNSRLFCYICRLRC 656 >At5g56440.1 68418.m07045 F-box family protein contains F-box domain Pfam:PF00646 Length = 430 Score = 27.9 bits (59), Expect = 7.4 Identities = 12/21 (57%), Positives = 15/21 (71%) Frame = +2 Query: 8 REAVPGDNVGFNVKNVSVKEL 70 R VPGD+VGF +K S+K L Sbjct: 210 RSQVPGDDVGFVIKAPSLKSL 230 >At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu, putative similar to mitochondrial elongation factor Tu [Arabidopsis thaliana] gi|1149571|emb|CAA61511 Length = 454 Score = 27.9 bits (59), Expect = 7.4 Identities = 26/85 (30%), Positives = 39/85 (45%), Gaps = 6/85 (7%) Frame = +2 Query: 23 GDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVL------NHPGQISNGYT 184 GDNVG ++ + ++++RG V +K K F A++ VL H SN Y Sbjct: 332 GDNVGLLLRGLKREDIQRGMVI--AKPGSCKTYKKFEAEIYVLTKDEGGRHTAFFSN-YR 388 Query: 185 PVLDCXTAHIACKFAEIKEKVDRLL 259 P TA I K E+ E V ++ Sbjct: 389 PQFYLRTADITGK-VELPENVKMVM 412 >At1g55380.1 68414.m06334 DC1 domain-containing protein contains Pfam profile PF03107: DC1 domain Length = 661 Score = 27.9 bits (59), Expect = 7.4 Identities = 14/38 (36%), Positives = 21/38 (55%), Gaps = 5/38 (13%) Frame = +2 Query: 602 IFYKACN---VTLFYNLYKVIHNI--SETFCYDCKLKC 700 I+ K C+ V L+Y ++ N S FCY C+L+C Sbjct: 579 IYLKPCHIFKVGLYYKEVEIARNDGNSRLFCYTCELRC 616 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,440,534 Number of Sequences: 28952 Number of extensions: 314809 Number of successful extensions: 889 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 848 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 888 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1614253080 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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