BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00488 (737 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearl... 32 0.35 At3g26610.1 68416.m03322 polygalacturonase, putative / pectinase... 30 1.4 At4g18180.1 68417.m02701 glycoside hydrolase family 28 protein /... 29 2.4 At3g19570.2 68416.m02482 expressed protein contains Pfam domain,... 27 9.8 At3g19570.1 68416.m02481 expressed protein contains Pfam domain,... 27 9.8 At3g06770.3 68416.m00803 glycoside hydrolase family 28 protein /... 27 9.8 At3g06770.2 68416.m00804 glycoside hydrolase family 28 protein /... 27 9.8 At3g06770.1 68416.m00802 glycoside hydrolase family 28 protein /... 27 9.8 At3g06290.1 68416.m00722 SAC3/GANP family protein contains Pfam ... 27 9.8 >At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearly identical to ubiquitin-protein ligase 1 [Arabidopsis thaliana] GI:7108521; E3, HECT-domain protein family; similar to GI:7108521, GB:AAF36454 from [Arabidopsis thaliana] Length = 3891 Score = 32.3 bits (70), Expect = 0.35 Identities = 18/43 (41%), Positives = 22/43 (51%) Frame = -2 Query: 496 RNFRLKGKIIHSFSNICKFQKQKKKHFNARTTLTPLEIKYLPL 368 RN L GKII S + F+ K K F T PLE+ + PL Sbjct: 241 RNRSLDGKIITSLAKFMVFEPSKIKSFINSVTSVPLELIHEPL 283 >At3g26610.1 68416.m03322 polygalacturonase, putative / pectinase, putative similar to polygalacturonase (PG1) GI:5669846, (PG2) GI:5669848 [Glycine max]; contains PF00295: Glycosyl hydrolases family 28 (polygalacturonases) Length = 470 Score = 30.3 bits (65), Expect = 1.4 Identities = 11/24 (45%), Positives = 18/24 (75%) Frame = +3 Query: 477 PFSLKFLTSNNVVVMRVTLINQPL 548 P +L+F +SNNV V ++++N PL Sbjct: 193 PTALRFYSSNNVTVRDISIVNSPL 216 >At4g18180.1 68417.m02701 glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein similar to polygalacturonase [Salix gilgiana] GI:6714524; contains PF00295: Glycosyl hydrolases family 28 (polygalacturonases) Length = 414 Score = 29.5 bits (63), Expect = 2.4 Identities = 12/25 (48%), Positives = 17/25 (68%) Frame = +3 Query: 465 CIILPFSLKFLTSNNVVVMRVTLIN 539 C +LP SLKF+ N VV R++ +N Sbjct: 150 CKLLPTSLKFVGMNRTVVRRISSVN 174 >At3g19570.2 68416.m02482 expressed protein contains Pfam domain, PF04484: Family of unknown function (DUF566) Length = 644 Score = 27.5 bits (58), Expect = 9.8 Identities = 13/42 (30%), Positives = 20/42 (47%) Frame = -2 Query: 157 SDTPGICSGHSSGEALSSFSELKRLSSKDVNGISSDDAWSST 32 SDT + SG ++G E+ + S NG++S W T Sbjct: 290 SDTDSVSSGSTNGAHECGSGEVSKTRSLPRNGMASTKFWQET 331 >At3g19570.1 68416.m02481 expressed protein contains Pfam domain, PF04484: Family of unknown function (DUF566) Length = 627 Score = 27.5 bits (58), Expect = 9.8 Identities = 13/42 (30%), Positives = 20/42 (47%) Frame = -2 Query: 157 SDTPGICSGHSSGEALSSFSELKRLSSKDVNGISSDDAWSST 32 SDT + SG ++G E+ + S NG++S W T Sbjct: 290 SDTDSVSSGSTNGAHECGSGEVSKTRSLPRNGMASTKFWQET 331 >At3g06770.3 68416.m00803 glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein weak similarity to polygalacturonase [Persea americana] GI:166951; contains PF00295: Glycosyl hydrolases family 28 (polygalacturonases) Length = 377 Score = 27.5 bits (58), Expect = 9.8 Identities = 10/23 (43%), Positives = 16/23 (69%) Frame = +3 Query: 477 PFSLKFLTSNNVVVMRVTLINQP 545 P ++FL+S NV+V +T +N P Sbjct: 110 PHLIEFLSSENVIVSNLTFLNAP 132 >At3g06770.2 68416.m00804 glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein weak similarity to polygalacturonase [Persea americana] GI:166951; contains PF00295: Glycosyl hydrolases family 28 (polygalacturonases) Length = 446 Score = 27.5 bits (58), Expect = 9.8 Identities = 10/23 (43%), Positives = 16/23 (69%) Frame = +3 Query: 477 PFSLKFLTSNNVVVMRVTLINQP 545 P ++FL+S NV+V +T +N P Sbjct: 179 PHLIEFLSSENVIVSNLTFLNAP 201 >At3g06770.1 68416.m00802 glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein weak similarity to polygalacturonase [Persea americana] GI:166951; contains PF00295: Glycosyl hydrolases family 28 (polygalacturonases) Length = 377 Score = 27.5 bits (58), Expect = 9.8 Identities = 10/23 (43%), Positives = 16/23 (69%) Frame = +3 Query: 477 PFSLKFLTSNNVVVMRVTLINQP 545 P ++FL+S NV+V +T +N P Sbjct: 110 PHLIEFLSSENVIVSNLTFLNAP 132 >At3g06290.1 68416.m00722 SAC3/GANP family protein contains Pfam profile: PF03399 SAC3/GANP family Length = 1720 Score = 27.5 bits (58), Expect = 9.8 Identities = 13/38 (34%), Positives = 24/38 (63%) Frame = -1 Query: 338 FHGSSL*LLPGPVDKLSHEVFLLP**DHRCKCSKLLHV 225 +HG S+ + P + +++FL D++ KCSKL+H+ Sbjct: 798 YHGFSIKVFEEPY-MVKNDLFLHADKDYKTKCSKLVHM 834 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,757,129 Number of Sequences: 28952 Number of extensions: 288166 Number of successful extensions: 681 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 672 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 681 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1624036432 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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