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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00488
         (737 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearl...    32   0.35 
At3g26610.1 68416.m03322 polygalacturonase, putative / pectinase...    30   1.4  
At4g18180.1 68417.m02701 glycoside hydrolase family 28 protein /...    29   2.4  
At3g19570.2 68416.m02482 expressed protein contains Pfam domain,...    27   9.8  
At3g19570.1 68416.m02481 expressed protein contains Pfam domain,...    27   9.8  
At3g06770.3 68416.m00803 glycoside hydrolase family 28 protein /...    27   9.8  
At3g06770.2 68416.m00804 glycoside hydrolase family 28 protein /...    27   9.8  
At3g06770.1 68416.m00802 glycoside hydrolase family 28 protein /...    27   9.8  
At3g06290.1 68416.m00722 SAC3/GANP family protein contains Pfam ...    27   9.8  

>At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearly
           identical to ubiquitin-protein ligase 1 [Arabidopsis
           thaliana] GI:7108521; E3, HECT-domain protein family;
           similar to GI:7108521, GB:AAF36454 from [Arabidopsis
           thaliana]
          Length = 3891

 Score = 32.3 bits (70), Expect = 0.35
 Identities = 18/43 (41%), Positives = 22/43 (51%)
 Frame = -2

Query: 496 RNFRLKGKIIHSFSNICKFQKQKKKHFNARTTLTPLEIKYLPL 368
           RN  L GKII S +    F+  K K F    T  PLE+ + PL
Sbjct: 241 RNRSLDGKIITSLAKFMVFEPSKIKSFINSVTSVPLELIHEPL 283


>At3g26610.1 68416.m03322 polygalacturonase, putative / pectinase,
           putative similar to polygalacturonase (PG1) GI:5669846,
           (PG2) GI:5669848 [Glycine max]; contains PF00295:
           Glycosyl hydrolases family 28 (polygalacturonases)
          Length = 470

 Score = 30.3 bits (65), Expect = 1.4
 Identities = 11/24 (45%), Positives = 18/24 (75%)
 Frame = +3

Query: 477 PFSLKFLTSNNVVVMRVTLINQPL 548
           P +L+F +SNNV V  ++++N PL
Sbjct: 193 PTALRFYSSNNVTVRDISIVNSPL 216


>At4g18180.1 68417.m02701 glycoside hydrolase family 28 protein /
           polygalacturonase (pectinase) family protein similar to
           polygalacturonase [Salix gilgiana] GI:6714524; contains
           PF00295: Glycosyl hydrolases family 28
           (polygalacturonases)
          Length = 414

 Score = 29.5 bits (63), Expect = 2.4
 Identities = 12/25 (48%), Positives = 17/25 (68%)
 Frame = +3

Query: 465 CIILPFSLKFLTSNNVVVMRVTLIN 539
           C +LP SLKF+  N  VV R++ +N
Sbjct: 150 CKLLPTSLKFVGMNRTVVRRISSVN 174


>At3g19570.2 68416.m02482 expressed protein contains Pfam domain,
           PF04484: Family of unknown function (DUF566)
          Length = 644

 Score = 27.5 bits (58), Expect = 9.8
 Identities = 13/42 (30%), Positives = 20/42 (47%)
 Frame = -2

Query: 157 SDTPGICSGHSSGEALSSFSELKRLSSKDVNGISSDDAWSST 32
           SDT  + SG ++G       E+ +  S   NG++S   W  T
Sbjct: 290 SDTDSVSSGSTNGAHECGSGEVSKTRSLPRNGMASTKFWQET 331


>At3g19570.1 68416.m02481 expressed protein contains Pfam domain,
           PF04484: Family of unknown function (DUF566)
          Length = 627

 Score = 27.5 bits (58), Expect = 9.8
 Identities = 13/42 (30%), Positives = 20/42 (47%)
 Frame = -2

Query: 157 SDTPGICSGHSSGEALSSFSELKRLSSKDVNGISSDDAWSST 32
           SDT  + SG ++G       E+ +  S   NG++S   W  T
Sbjct: 290 SDTDSVSSGSTNGAHECGSGEVSKTRSLPRNGMASTKFWQET 331


>At3g06770.3 68416.m00803 glycoside hydrolase family 28 protein /
           polygalacturonase (pectinase) family protein weak
           similarity to polygalacturonase [Persea americana]
           GI:166951; contains PF00295: Glycosyl hydrolases family
           28 (polygalacturonases)
          Length = 377

 Score = 27.5 bits (58), Expect = 9.8
 Identities = 10/23 (43%), Positives = 16/23 (69%)
 Frame = +3

Query: 477 PFSLKFLTSNNVVVMRVTLINQP 545
           P  ++FL+S NV+V  +T +N P
Sbjct: 110 PHLIEFLSSENVIVSNLTFLNAP 132


>At3g06770.2 68416.m00804 glycoside hydrolase family 28 protein /
           polygalacturonase (pectinase) family protein weak
           similarity to polygalacturonase [Persea americana]
           GI:166951; contains PF00295: Glycosyl hydrolases family
           28 (polygalacturonases)
          Length = 446

 Score = 27.5 bits (58), Expect = 9.8
 Identities = 10/23 (43%), Positives = 16/23 (69%)
 Frame = +3

Query: 477 PFSLKFLTSNNVVVMRVTLINQP 545
           P  ++FL+S NV+V  +T +N P
Sbjct: 179 PHLIEFLSSENVIVSNLTFLNAP 201


>At3g06770.1 68416.m00802 glycoside hydrolase family 28 protein /
           polygalacturonase (pectinase) family protein weak
           similarity to polygalacturonase [Persea americana]
           GI:166951; contains PF00295: Glycosyl hydrolases family
           28 (polygalacturonases)
          Length = 377

 Score = 27.5 bits (58), Expect = 9.8
 Identities = 10/23 (43%), Positives = 16/23 (69%)
 Frame = +3

Query: 477 PFSLKFLTSNNVVVMRVTLINQP 545
           P  ++FL+S NV+V  +T +N P
Sbjct: 110 PHLIEFLSSENVIVSNLTFLNAP 132


>At3g06290.1 68416.m00722 SAC3/GANP family protein contains Pfam
           profile: PF03399 SAC3/GANP family
          Length = 1720

 Score = 27.5 bits (58), Expect = 9.8
 Identities = 13/38 (34%), Positives = 24/38 (63%)
 Frame = -1

Query: 338 FHGSSL*LLPGPVDKLSHEVFLLP**DHRCKCSKLLHV 225
           +HG S+ +   P   + +++FL    D++ KCSKL+H+
Sbjct: 798 YHGFSIKVFEEPY-MVKNDLFLHADKDYKTKCSKLVHM 834


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,757,129
Number of Sequences: 28952
Number of extensions: 288166
Number of successful extensions: 681
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 672
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 681
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1624036432
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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