Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00485
(716 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g06830.1 68418.m00772 expressed protein contains Pfam profile... 64 7e-11
At1g67230.1 68414.m07652 expressed protein 31 0.76
At4g24280.1 68417.m03486 heat shock protein 70, putative / HSP70... 30 1.8
At1g34140.1 68414.m04235 polyadenylate-binding protein, putative... 30 1.8
At1g47550.1 68414.m05278 expressed protein 29 2.3
At2g39380.1 68415.m04833 exocyst subunit EXO70 family protein co... 29 3.1
At5g38850.1 68418.m04699 disease resistance protein (TIR-NBS-LRR... 29 4.1
At5g10360.1 68418.m01202 40S ribosomal protein S6 (RPS6B) 28 5.4
At4g31700.1 68417.m04500 40S ribosomal protein S6 (RPS6A) riboso... 28 5.4
At2g27280.1 68415.m03278 hypothetical protein 28 5.4
At1g22830.1 68414.m02850 pentatricopeptide (PPR) repeat-containi... 28 5.4
At1g20400.1 68414.m02544 myosin heavy chain-related 28 5.4
At5g66440.1 68418.m08379 expressed protein 28 7.1
At3g56300.1 68416.m06258 tRNA synthetase class I (C) family prot... 27 9.4
At1g28210.2 68414.m03463 DNAJ heat shock protein, putative stron... 27 9.4
At1g28210.1 68414.m03462 DNAJ heat shock protein, putative stron... 27 9.4
At1g22300.3 68414.m02790 14-3-3 protein GF14 epsilon (GRF10) ide... 27 9.4
At1g22300.2 68414.m02789 14-3-3 protein GF14 epsilon (GRF10) ide... 27 9.4
At1g22300.1 68414.m02788 14-3-3 protein GF14 epsilon (GRF10) ide... 27 9.4
>At5g06830.1 68418.m00772 expressed protein contains Pfam profile:
PF05600 protein of unknown function (DUF773)
Length = 549
Score = 64.5 bits (150), Expect = 7e-11
Identities = 26/74 (35%), Positives = 47/74 (63%)
Frame = +3
Query: 249 QEISYEIPGLKRQIAKEEQAQADFEKKHADYLKNEASSKSEFLALCKQLGIQGEKIKREL 428
Q ++YEIP LK+Q+ K +Q + ++K AD ++ A S +++ C++LG+QG ++REL
Sbjct: 123 QNVNYEIPYLKKQVQKVQQQMTELDRKEADIKRSVALSATKYEDACRELGLQGNNVRREL 182
Query: 429 VAKLQELPEIYDQI 470
+ LP + +I
Sbjct: 183 LETASSLPSTFSKI 196
Score = 50.4 bits (115), Expect = 1e-06
Identities = 27/84 (32%), Positives = 45/84 (53%), Gaps = 1/84 (1%)
Frame = +1
Query: 10 KINNAIQDMPAHQD-IAALLSGSYINYFHCLKIIEILKETEADTKNLFGRYGSQRMKDWQ 186
KI +P D L I Y K+ EIL +T +++N+FGR S W+
Sbjct: 43 KILKEFSSLPKEIDPFFQTLDPEVIGYLEVKKVYEILLKTTPESRNIFGRL-SGASGVWE 101
Query: 187 DVERNYEKENLYLAEAAQMLVRKL 258
+ R +EK+++YL EAAQ++++ +
Sbjct: 102 AIVRAFEKDHIYLGEAAQIIIQNV 125
>At1g67230.1 68414.m07652 expressed protein
Length = 1132
Score = 31.1 bits (67), Expect = 0.76
Identities = 22/68 (32%), Positives = 34/68 (50%), Gaps = 2/68 (2%)
Frame = +3
Query: 249 QEISYEIPGLKRQIAKEEQAQADFEKKHADYLKNEASSKSEFLALCKQLGIQGEKIK--R 422
+EI + L +++ E QAQ K D L+ +SE+L L +L Q EK + +
Sbjct: 445 KEIILNLKALVEKVSGENQAQLSEINKEKDELRVTEEERSEYLRLQTELKEQIEKCRSQQ 504
Query: 423 ELVAKLQE 446
EL+ K E
Sbjct: 505 ELLQKEAE 512
>At4g24280.1 68417.m03486 heat shock protein 70, putative / HSP70,
putative strong similarity to heat shock protein 70
[Arabidopsis thaliana] GI:6746592; similar to heat shock
70 protein - Spinacia oleracea,PID:g2654208
Length = 718
Score = 29.9 bits (64), Expect = 1.8
Identities = 24/60 (40%), Positives = 33/60 (55%), Gaps = 1/60 (1%)
Frame = +3
Query: 297 EEQAQADFEKKHADYLKNEASSKSEFLALCKQLGIQGEKIKRELVAKLQ-ELPEIYDQIG 473
E A+ D EK+ A KN+A S KQL GEKI E+ K++ +L E+ D+IG
Sbjct: 591 ERFAKDDKEKRDAIDTKNQADSV--VYQTEKQLKELGEKIPGEVKEKVEAKLQELKDKIG 648
>At1g34140.1 68414.m04235 polyadenylate-binding protein, putative /
PABP, putative non-consensus splice donor TA at exon 1;
similar to polyadenylate-binding protein
(poly(A)-binding protein) from [Triticum aestivum]
GI:1737492, [Nicotiana tabacum] GI:7673355, {Arabidopsis
thaliana} SP|P42731; contains InterPro entry IPR000504:
RNA-binding region RNP-1 (RNA recognition motif) (RRM)
Length = 407
Score = 29.9 bits (64), Expect = 1.8
Identities = 18/49 (36%), Positives = 25/49 (51%), Gaps = 4/49 (8%)
Frame = +1
Query: 124 TEADTKNLFGRYG----SQRMKDWQDVERNYEKENLYLAEAAQMLVRKL 258
T+AD K LFG +G + MKD + R + N AEAA + K+
Sbjct: 131 TDADLKRLFGEFGEITSAVVMKDGEGKSRRFGFVNFEKAEAAVTAIEKM 179
>At1g47550.1 68414.m05278 expressed protein
Length = 887
Score = 29.5 bits (63), Expect = 2.3
Identities = 22/91 (24%), Positives = 45/91 (49%)
Frame = +3
Query: 276 LKRQIAKEEQAQADFEKKHADYLKNEASSKSEFLALCKQLGIQGEKIKRELVAKLQELPE 455
+K + A+ E+ +A F ++ +++L+N +S +F+ K Q ++KR A L+
Sbjct: 371 VKEKRAELEKLKATFVRRASEFLRNYFASLVDFMVSDKSYFSQRGQLKRPDHADLRYKCR 430
Query: 456 IYDQIGKSLKPLQPGIEFIMHSQSISSVRIL 548
Y ++ + LK L + SS+ +L
Sbjct: 431 TYARLLQHLKGLDKNCLGPLRKAYCSSLNLL 461
>At2g39380.1 68415.m04833 exocyst subunit EXO70 family protein
contains Pfam domain PF03081: Exo70 exocyst complex
subunit;
Length = 637
Score = 29.1 bits (62), Expect = 3.1
Identities = 25/81 (30%), Positives = 43/81 (53%), Gaps = 5/81 (6%)
Frame = +3
Query: 321 EKKHADYLKNEASSKSE--FLALCKQLGIQGEKIKRELVAKLQELPE-IYD--QIGKSLK 485
EK D++ + +S+ E F + + GI K ELVA+ + PE I+ + ++
Sbjct: 261 EKLLCDHVFSASSTIRESCFYEIVNEAGINLFKFP-ELVAEKKPSPERIFRLMDLYAAIS 319
Query: 486 PLQPGIEFIMHSQSISSVRIL 548
L+P IE I H S+++V+ L
Sbjct: 320 DLRPDIELIFHFDSVAAVKTL 340
>At5g38850.1 68418.m04699 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 986
Score = 28.7 bits (61), Expect = 4.1
Identities = 11/56 (19%), Positives = 24/56 (42%)
Frame = +1
Query: 76 YINYFHCLKIIEILKETEADTKNLFGRYGSQRMKDWQDVERNYEKENLYLAEAAQM 243
Y+N C ++ E+ + L YG R+K + D+ N ++ + ++
Sbjct: 665 YLNMMGCRRLKEVPPHINLKSLELVNMYGCSRLKSFPDISTNISSLDISYTDVEEL 720
>At5g10360.1 68418.m01202 40S ribosomal protein S6 (RPS6B)
Length = 249
Score = 28.3 bits (60), Expect = 5.4
Identities = 14/34 (41%), Positives = 20/34 (58%)
Frame = +3
Query: 279 KRQIAKEEQAQADFEKKHADYLKNEASSKSEFLA 380
K++IAK AD++K A LK + +SE LA
Sbjct: 200 KKRIAKANSDAADYQKLLASRLKEQRDRRSESLA 233
>At4g31700.1 68417.m04500 40S ribosomal protein S6 (RPS6A) ribosomal
protein S6, Arabidopsis thaliana, PID:g2662469
Length = 250
Score = 28.3 bits (60), Expect = 5.4
Identities = 14/34 (41%), Positives = 20/34 (58%)
Frame = +3
Query: 279 KRQIAKEEQAQADFEKKHADYLKNEASSKSEFLA 380
K++IAK AD++K A LK + +SE LA
Sbjct: 200 KKKIAKANSDAADYQKLLASRLKEQRDRRSESLA 233
>At2g27280.1 68415.m03278 hypothetical protein
Length = 427
Score = 28.3 bits (60), Expect = 5.4
Identities = 22/77 (28%), Positives = 40/77 (51%)
Frame = +3
Query: 252 EISYEIPGLKRQIAKEEQAQADFEKKHADYLKNEASSKSEFLALCKQLGIQGEKIKRELV 431
EI YE L ++ AK+E +D EK K + + ++L ++ ++ + +R+ V
Sbjct: 255 EIVYERK-LAKERAKDEHLYSDKEKFVTGPFKRKLEEQKKWL---EEERLRELREERDDV 310
Query: 432 AKLQELPEIYDQIGKSL 482
K +L E Y IGK++
Sbjct: 311 TKKNDLSEFYINIGKNV 327
>At1g22830.1 68414.m02850 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat. Gene
continues on the 3' end of BAC F19G10 gb|AF000657 gene
F19G10.21
Length = 703
Score = 28.3 bits (60), Expect = 5.4
Identities = 13/32 (40%), Positives = 20/32 (62%)
Frame = +2
Query: 563 ILQYVIIHGNTTVYEWSYGEPPLSLEPDPIHI 658
+L+ +IHGNT + EW+ + L LE P H+
Sbjct: 599 LLKACLIHGNTNIGEWAADK--LLLETKPEHL 628
>At1g20400.1 68414.m02544 myosin heavy chain-related
Length = 944
Score = 28.3 bits (60), Expect = 5.4
Identities = 23/99 (23%), Positives = 46/99 (46%), Gaps = 3/99 (3%)
Frame = +3
Query: 276 LKRQIAKEEQAQADFEKKHADYLKNEASSKSEFLALCKQLGIQGEKIKR---ELVAKLQE 446
L+ +++ E++ + KK K A KSE L Q + GE+ R +L + ++
Sbjct: 708 LRGELSAEKKKNDELLKKLESASKEAAHLKSEVATLAYQRTVMGEERDRCTLDLEKEREK 767
Query: 447 LPEIYDQIGKSLKPLQPGIEFIMHSQSISSVRILQKLYQ 563
E+ D++ K L+ E +Q+ ++ + L+Q
Sbjct: 768 TVELEDRLKSEKKRLRSRREKYAENQTSKALIHVADLFQ 806
>At5g66440.1 68418.m08379 expressed protein
Length = 252
Score = 27.9 bits (59), Expect = 7.1
Identities = 13/70 (18%), Positives = 27/70 (38%)
Frame = +2
Query: 479 TKTTPARYRVYNAFTKYILGEDSPEALPILQYVIIHGNTTVYEWSYGEPPLSLEPDPIHI 658
T A +Y AF + + P + + ++ + P L+++ + H+
Sbjct: 94 TAVASAETEIYQAFVRSVENRSKPSDEDLTEGIVYDDKQVIVTDLDSNPNLTVDENMPHV 153
Query: 659 ELDDDEEQAS 688
E+D D S
Sbjct: 154 EIDSDSSPHS 163
>At3g56300.1 68416.m06258 tRNA synthetase class I (C) family protein
similar to cysteinyl-tRNA synthetase [Methanococcus
maripaludis] GI:6599476; contains Pfam profile PF01406:
tRNA synthetases class I (C)
Length = 489
Score = 27.5 bits (58), Expect = 9.4
Identities = 16/43 (37%), Positives = 22/43 (51%), Gaps = 1/43 (2%)
Frame = -3
Query: 504 LYLAGVVLVISLFGHISQAAPAVS-PLILS*SSHLGYQVVYTK 379
+Y+ G+ + HI A AVS L+ HLGYQV Y +
Sbjct: 34 IYVCGITAYD--YSHIGHARAAVSFDLLYRYLRHLGYQVTYVR 74
>At1g28210.2 68414.m03463 DNAJ heat shock protein, putative strong
similarity to mitochondrial DnaJ protein (AtJ1)
GI:564030 from [Arabidopsis thaliana]; contains Pfam
profiles PF00226: DnaJ domain, PF01556: DnaJ C terminal
region, PF00684: DnaJ central domain (4 repeats);
identical to cDNA DnaJ homolog AtJ1 (atj) GI:564029
Length = 427
Score = 27.5 bits (58), Expect = 9.4
Identities = 19/78 (24%), Positives = 36/78 (46%), Gaps = 3/78 (3%)
Frame = +3
Query: 219 VFSRGSTDAGQEISYEIPGLKRQIAKEEQAQADFE---KKHADYLKNEASSKSEFLALCK 389
+ + G ++ Y++ G+ + +EE ++ E K H D +N S+K +F + +
Sbjct: 36 IHATGINNSSARNYYDVLGVSPKATREEIKKSFHELAKKFHPDTNRNNPSAKRKFQEIRE 95
Query: 390 QLGIQGEKIKRELVAKLQ 443
G +RE KLQ
Sbjct: 96 AYETLGNSERREEYDKLQ 113
>At1g28210.1 68414.m03462 DNAJ heat shock protein, putative strong
similarity to mitochondrial DnaJ protein (AtJ1)
GI:564030 from [Arabidopsis thaliana]; contains Pfam
profiles PF00226: DnaJ domain, PF01556: DnaJ C terminal
region, PF00684: DnaJ central domain (4 repeats);
identical to cDNA DnaJ homolog AtJ1 (atj) GI:564029
Length = 408
Score = 27.5 bits (58), Expect = 9.4
Identities = 19/78 (24%), Positives = 36/78 (46%), Gaps = 3/78 (3%)
Frame = +3
Query: 219 VFSRGSTDAGQEISYEIPGLKRQIAKEEQAQADFE---KKHADYLKNEASSKSEFLALCK 389
+ + G ++ Y++ G+ + +EE ++ E K H D +N S+K +F + +
Sbjct: 36 IHATGINNSSARNYYDVLGVSPKATREEIKKSFHELAKKFHPDTNRNNPSAKRKFQEIRE 95
Query: 390 QLGIQGEKIKRELVAKLQ 443
G +RE KLQ
Sbjct: 96 AYETLGNSERREEYDKLQ 113
>At1g22300.3 68414.m02790 14-3-3 protein GF14 epsilon (GRF10)
identical to 14-3-3 protein GF14 epsilon GI:5802798,
SP:P48347 from [Arabidopsis thaliana]
Length = 251
Score = 27.5 bits (58), Expect = 9.4
Identities = 14/48 (29%), Positives = 30/48 (62%), Gaps = 1/48 (2%)
Frame = +3
Query: 399 IQGEKIKRELVAKLQELPEIYDQIGKSLKPL-QPGIEFIMHSQSISSV 539
++ E+ K+ +AKL E E YD++ +++K + Q +E + +++ SV
Sbjct: 1 MENEREKQVYLAKLSEQTERYDEMVEAMKKVAQLDVELTVEERNLVSV 48
>At1g22300.2 68414.m02789 14-3-3 protein GF14 epsilon (GRF10)
identical to 14-3-3 protein GF14 epsilon GI:5802798,
SP:P48347 from [Arabidopsis thaliana]
Length = 254
Score = 27.5 bits (58), Expect = 9.4
Identities = 14/48 (29%), Positives = 30/48 (62%), Gaps = 1/48 (2%)
Frame = +3
Query: 399 IQGEKIKRELVAKLQELPEIYDQIGKSLKPL-QPGIEFIMHSQSISSV 539
++ E+ K+ +AKL E E YD++ +++K + Q +E + +++ SV
Sbjct: 1 MENEREKQVYLAKLSEQTERYDEMVEAMKKVAQLDVELTVEERNLVSV 48
>At1g22300.1 68414.m02788 14-3-3 protein GF14 epsilon (GRF10)
identical to 14-3-3 protein GF14 epsilon GI:5802798,
SP:P48347 from [Arabidopsis thaliana]
Length = 254
Score = 27.5 bits (58), Expect = 9.4
Identities = 14/48 (29%), Positives = 30/48 (62%), Gaps = 1/48 (2%)
Frame = +3
Query: 399 IQGEKIKRELVAKLQELPEIYDQIGKSLKPL-QPGIEFIMHSQSISSV 539
++ E+ K+ +AKL E E YD++ +++K + Q +E + +++ SV
Sbjct: 1 MENEREKQVYLAKLSEQTERYDEMVEAMKKVAQLDVELTVEERNLVSV 48
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,272,118
Number of Sequences: 28952
Number of extensions: 288803
Number of successful extensions: 1002
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 971
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1001
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1555552968
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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