BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00464 (718 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC19B12.11c |||zinc finger protein|Schizosaccharomyces pombe|c... 67 3e-12 SPBC3B8.05 |||diphthamide biosynthesis protein |Schizosaccharomy... 30 0.38 SPBC660.17c |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 28 1.5 SPBC3D6.04c |mad1||mitotic spindle checkpoint protein Mad1|Schiz... 27 2.7 SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 27 2.7 SPBC725.07 |pex5||peroxisomal targeting signal receptor |Schizos... 27 3.5 SPAC13G6.09 |||zf-MYND type |Schizosaccharomyces pombe|chr 1|||M... 26 4.7 SPBC887.03c |noc3||Noc2p-Noc3p complex subunit Noc3 |Schizosacch... 26 4.7 SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|... 26 6.2 SPAC869.11 ||SPAC922.08c|amino acid permease, unknown 6|Schizosa... 26 6.2 SPAC16E8.07c |vph1||V-type ATPase subunit a|Schizosaccharomyces ... 26 6.2 SPCC364.05 |vps3||GTPase regulator Vps3 |Schizosaccharomyces pom... 26 6.2 SPAC1D4.07c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 25 8.2 SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom... 25 8.2 SPBC20F10.06 |mad2||spindle checkpoint protein Mad2|Schizosaccha... 25 8.2 SPBP35G2.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr... 25 8.2 >SPAC19B12.11c |||zinc finger protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 124 Score = 66.9 bits (156), Expect = 3e-12 Identities = 36/77 (46%), Positives = 44/77 (57%) Frame = +3 Query: 30 KRKKYHCGDTHLKKRWRVRNRKKDLDEIDQDLKEENAEKLLNQKVDLDLPGAAQHYCLHC 209 +++K+H H R RV R DLD+I DL E +EK +D DLPG QHYC+ C Sbjct: 6 RKRKHHSNGNHALFRTRVYGR--DLDQIHNDLTE--SEKFDKLPIDPDLPGLGQHYCIEC 61 Query: 210 ARYFIDEQALNDHFKQK 260 ARYF QAL H K K Sbjct: 62 ARYFDSSQALLVHKKGK 78 Score = 40.7 bits (91), Expect = 2e-04 Identities = 20/43 (46%), Positives = 25/43 (58%) Frame = +2 Query: 251 QAKVHKRRLKALELEPYTIEESERAAGHGSFKTATKRKIVSQN 379 + KVHKRRLK L EPYT EE+E A G K + K+ + Sbjct: 76 KGKVHKRRLKNLREEPYTQEEAEAAVNIGQPKQSVASKLADNS 118 >SPBC3B8.05 |||diphthamide biosynthesis protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 436 Score = 29.9 bits (64), Expect = 0.38 Identities = 15/41 (36%), Positives = 21/41 (51%) Frame = +2 Query: 596 PEKFQNINNIKDDLKKRKYNYNFEKLEELWHVYEELKQKRI 718 PE N I + +K NYNFE + +WH+ K KR+ Sbjct: 53 PEDILNDKAINEAIKLLPQNYNFEIHKTIWHI-RLRKAKRV 92 >SPBC660.17c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 172 Score = 27.9 bits (59), Expect = 1.5 Identities = 12/26 (46%), Positives = 16/26 (61%) Frame = -2 Query: 180 LADLNQLFGSVIFQHFLPLNLGLFHP 103 L +L LF +FLPL +GLF+P Sbjct: 73 LVELLSLFYCAYVMYFLPLEVGLFNP 98 >SPBC3D6.04c |mad1||mitotic spindle checkpoint protein Mad1|Schizosaccharomyces pombe|chr 2|||Manual Length = 689 Score = 27.1 bits (57), Expect = 2.7 Identities = 13/37 (35%), Positives = 25/37 (67%) Frame = +2 Query: 602 KFQNINNIKDDLKKRKYNYNFEKLEELWHVYEELKQK 712 +FQ + N+K+DLK+++ + E++E + EE +QK Sbjct: 61 EFQ-LENLKNDLKRKELEFEREQIELQRKLAEEHEQK 96 >SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces pombe|chr 1|||Manual Length = 1957 Score = 27.1 bits (57), Expect = 2.7 Identities = 15/37 (40%), Positives = 21/37 (56%), Gaps = 2/37 (5%) Frame = +2 Query: 608 QNINNIKDDLKKRKYNYNFEKLE--ELWHVYEELKQK 712 +NI +K+DL+K + F KLE L V + LK K Sbjct: 689 KNIQTLKEDLRKSEEALRFSKLEAKNLREVIDNLKGK 725 >SPBC725.07 |pex5||peroxisomal targeting signal receptor |Schizosaccharomyces pombe|chr 2|||Manual Length = 598 Score = 26.6 bits (56), Expect = 3.5 Identities = 10/37 (27%), Positives = 21/37 (56%) Frame = +2 Query: 590 DFPEKFQNINNIKDDLKKRKYNYNFEKLEELWHVYEE 700 + +KF+ + I + L+K K + +EELW +++ Sbjct: 166 ELAKKFEEASQISNKLEKEKDATGSKSIEELWEEHQK 202 >SPAC13G6.09 |||zf-MYND type |Schizosaccharomyces pombe|chr 1|||Manual Length = 274 Score = 26.2 bits (55), Expect = 4.7 Identities = 15/30 (50%), Positives = 18/30 (60%), Gaps = 4/30 (13%) Frame = +3 Query: 108 EIDQDLKEENA----EKLLNQKVDLDLPGA 185 E+D + KEEN EKL N KVD + P A Sbjct: 121 EVDAEPKEENKKEIPEKLKNVKVDTENPSA 150 >SPBC887.03c |noc3||Noc2p-Noc3p complex subunit Noc3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 747 Score = 26.2 bits (55), Expect = 4.7 Identities = 10/24 (41%), Positives = 16/24 (66%) Frame = +3 Query: 108 EIDQDLKEENAEKLLNQKVDLDLP 179 EID D+K+E + L QK+ ++P Sbjct: 189 EIDSDIKDEKQKSLEEQKIAPEIP 212 >SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1687 Score = 25.8 bits (54), Expect = 6.2 Identities = 14/50 (28%), Positives = 24/50 (48%) Frame = +2 Query: 491 QNTKPIVTPKRYSALFINGAKATENYVYVTPYLDFPEKFQNINNIKDDLK 640 Q+ P++ + I+ K E+Y+ V + F E + I + K DLK Sbjct: 932 QSPNPVLKFHKLDGKIIDSFKTVEHYMEVLQPMIFMECWSQIQSTKLDLK 981 >SPAC869.11 ||SPAC922.08c|amino acid permease, unknown 6|Schizosaccharomyces pombe|chr 1|||Manual Length = 580 Score = 25.8 bits (54), Expect = 6.2 Identities = 12/29 (41%), Positives = 16/29 (55%) Frame = +1 Query: 529 SSFHQWCQGNRKLCLCHSLFRLSRKISEH 615 S+F W +CLCH +FRL+ K H Sbjct: 443 SNFFTW----GSICLCHIIFRLAFKKQGH 467 >SPAC16E8.07c |vph1||V-type ATPase subunit a|Schizosaccharomyces pombe|chr 1|||Manual Length = 805 Score = 25.8 bits (54), Expect = 6.2 Identities = 10/30 (33%), Positives = 18/30 (60%) Frame = -2 Query: 174 DLNQLFGSVIFQHFLPLNLGLFHPNLSFCY 85 DL+++ G V + ++ L +GLF + F Y Sbjct: 439 DLDEIVGMVFYGRYIVLLMGLFSMYVGFVY 468 >SPCC364.05 |vps3||GTPase regulator Vps3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 910 Score = 25.8 bits (54), Expect = 6.2 Identities = 13/30 (43%), Positives = 19/30 (63%), Gaps = 1/30 (3%) Frame = -3 Query: 665 QNYNCISFFSNHL-LYYLCSEIFLESLNKE 579 Q + +S FS HL YYLCS +E++ +E Sbjct: 717 QLWGDMSDFSTHLSFYYLCSINLMENVEEE 746 >SPAC1D4.07c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual Length = 146 Score = 25.4 bits (53), Expect = 8.2 Identities = 9/24 (37%), Positives = 13/24 (54%) Frame = +1 Query: 475 ISTHCSKHETYCNPKKIFSSFHQW 546 + THC K +TYC S+F + Sbjct: 112 LQTHCIKRKTYCVKHLAISNFQSF 135 >SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharomyces pombe|chr 2|||Manual Length = 1016 Score = 25.4 bits (53), Expect = 8.2 Identities = 10/34 (29%), Positives = 22/34 (64%) Frame = +3 Query: 84 RNRKKDLDEIDQDLKEENAEKLLNQKVDLDLPGA 185 + R+K +++ D +A+K++NQ++ + LP A Sbjct: 300 KQRRKKVEQEVSDKGWVSADKMINQRLSIFLPSA 333 >SPBC20F10.06 |mad2||spindle checkpoint protein Mad2|Schizosaccharomyces pombe|chr 2|||Manual Length = 203 Score = 25.4 bits (53), Expect = 8.2 Identities = 10/30 (33%), Positives = 19/30 (63%) Frame = +2 Query: 560 ENYVYVTPYLDFPEKFQNINNIKDDLKKRK 649 E + + +D ++FQNI N +D+L+ +K Sbjct: 97 ERWQFNVEMVDTADQFQNIGNKEDELRVQK 126 >SPBP35G2.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 1060 Score = 25.4 bits (53), Expect = 8.2 Identities = 13/38 (34%), Positives = 20/38 (52%) Frame = +2 Query: 605 FQNINNIKDDLKKRKYNYNFEKLEELWHVYEELKQKRI 718 + +++I+ K NF+ +E HV EEL KRI Sbjct: 610 YGTVSSIRMLSHKHSAFLNFDSVETAKHVLEELNGKRI 647 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,620,948 Number of Sequences: 5004 Number of extensions: 52457 Number of successful extensions: 223 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 211 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 222 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 335201398 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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