Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00453
(759 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 148 7e-37
SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 88 1e-18
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 80 3e-16
SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 78 1e-15
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 74 2e-14
SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 71 1e-13
SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 69 1e-12
SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 68 1e-12
SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 67 3e-12
SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 61 1e-10
SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 61 2e-10
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 56 5e-09
SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 48 2e-06
SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos... 33 0.058
SPCC1682.11c |||DUF580 family protein|Schizosaccharomyces pombe|... 28 1.7
SPBC1861.02 |abp2||ARS binding protein Abp2|Schizosaccharomyces ... 27 2.9
SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces ... 27 2.9
SPBC16G5.15c |fkh2||fork head transcription factor Fkh2 |Schizos... 25 8.9
SPCP1E11.05c |||sterol O-acyltransferase |Schizosaccharomyces po... 25 8.9
>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
Hus5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 148 bits (359), Expect = 7e-37
Identities = 62/85 (72%), Positives = 73/85 (85%)
Frame = +1
Query: 253 MNLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLS 432
++LM W+ IPGK T WEGGLYKL M F ++YP+ PPKC+F PPLFHPNVYPSGTVCLS
Sbjct: 36 LDLMNWKVGIPGKPKTSWEGGLYKLTMAFPEEYPTRPPKCRFTPPLFHPNVYPSGTVCLS 95
Query: 433 LLDEEKDWRPAITIKQILLGIQDLL 507
+L+EE+ W+PAITIKQILLGIQDLL
Sbjct: 96 ILNEEEGWKPAITIKQILLGIQDLL 120
Score = 53.6 bits (123), Expect = 3e-08
Identities = 21/34 (61%), Positives = 25/34 (73%)
Frame = +2
Query: 149 MSGIASARLAEERKAWRKDHPFGFVARPMKNPDG 250
MS + RL EERK WR+DHPFGF A+P K+ DG
Sbjct: 1 MSSLCKTRLQEERKQWRRDHPFGFYAKPCKSSDG 34
Score = 35.9 bits (79), Expect = 0.006
Identities = 12/25 (48%), Positives = 21/25 (84%)
Frame = +3
Query: 510 EPNVKDPAQAEAYTIYCQNRLEYDK 584
+PN+ PAQ EAYT++ ++++EY+K
Sbjct: 122 DPNIASPAQTEAYTMFKKDKVEYEK 146
>SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme
Ubc3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 166
Score = 87.8 bits (208), Expect = 1e-18
Identities = 41/95 (43%), Positives = 53/95 (55%), Gaps = 11/95 (11%)
Frame = +1
Query: 256 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSL 435
+ TW+C I G GTP+EGGLY + F DYP PP KFE FHPNVY GTVC+S+
Sbjct: 34 DFFTWDCLIQGPDGTPFEGGLYPATLKFPSDYPLGPPTLKFECEFFHPNVYKDGTVCISI 93
Query: 436 LDEEKD-----------WRPAITIKQILLGIQDLL 507
L D W P ++++ILL + +L
Sbjct: 94 LHAPGDDPNMYESSSERWSPVQSVEKILLSVMSML 128
>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 151
Score = 80.2 bits (189), Expect = 3e-16
Identities = 36/84 (42%), Positives = 48/84 (57%)
Frame = +1
Query: 256 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSL 435
N+M W I G TP+E G +KL + F + YP+ PP KF +FHPNVY +G +CL +
Sbjct: 32 NVMLWNAVIIGPADTPFEDGTFKLVLSFDEQYPNKPPLVKFVSTMFHPNVYANGELCLDI 91
Query: 436 LDEEKDWRPAITIKQILLGIQDLL 507
L + W P + IL IQ LL
Sbjct: 92 L--QNRWSPTYDVAAILTSIQSLL 113
Score = 25.8 bits (54), Expect = 6.7
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = +3
Query: 507 HEPNVKDPAQAEAYTIYCQNRLEY 578
++PN PA AEA ++ +N+ EY
Sbjct: 114 NDPNNASPANAEAAQLHRENKKEY 137
>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 147
Score = 78.2 bits (184), Expect = 1e-15
Identities = 33/84 (39%), Positives = 52/84 (61%)
Frame = +1
Query: 256 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSL 435
+L W+ I G +P+ GG++ L + F DYP PPK F ++HPN+ +G++CL +
Sbjct: 29 DLFHWQATIMGPADSPYAGGVFFLSIHFPTDYPFKPPKVNFTTRIYHPNINSNGSICLDI 88
Query: 436 LDEEKDWRPAITIKQILLGIQDLL 507
L ++ W PA+TI ++LL I LL
Sbjct: 89 LRDQ--WSPALTISKVLLSICSLL 110
>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
Length = 148
Score = 74.1 bits (174), Expect = 2e-14
Identities = 33/84 (39%), Positives = 51/84 (60%)
Frame = +1
Query: 256 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSL 435
NL ++ + G + + +EGG + L + D+YP PP +F ++HPNV G +CLS
Sbjct: 30 NLRYFKITMEGPQQSAYEGGKFHLELFLPDEYPMMPPNVRFLTKIYHPNVDKLGRICLST 89
Query: 436 LDEEKDWRPAITIKQILLGIQDLL 507
L +KDW PA+ I+ +LL IQ L+
Sbjct: 90 L--KKDWSPALQIRTVLLSIQALM 111
>SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 176
Score = 71.3 bits (167), Expect = 1e-13
Identities = 34/84 (40%), Positives = 48/84 (57%)
Frame = +1
Query: 256 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSL 435
NL+ W I G T +EG +K+ M F +YP SPP F P++HPNV SG +CL +
Sbjct: 57 NLLHWAGTITGPSDTYYEGLKFKISMSFPANYPYSPPTIIFTSPMWHPNVDMSGNICLDI 116
Query: 436 LDEEKDWRPAITIKQILLGIQDLL 507
L ++ W ++ ILL +Q LL
Sbjct: 117 LKDK--WSAVYNVQTILLSLQSLL 138
>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
Length = 167
Score = 68.5 bits (160), Expect = 1e-12
Identities = 28/66 (42%), Positives = 40/66 (60%)
Frame = +1
Query: 256 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSL 435
++ WE I G + T +EGG + + F DYP PPK KF ++HPNV+P+G VC+S+
Sbjct: 34 SIFEWEVMIIGPEDTLYEGGFFHATLSFPQDYPLMPPKMKFTTEIWHPNVHPNGEVCISI 93
Query: 436 LDEEKD 453
L D
Sbjct: 94 LHPPGD 99
>SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme
Ubc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 217
Score = 68.1 bits (159), Expect = 1e-12
Identities = 30/75 (40%), Positives = 46/75 (61%), Gaps = 1/75 (1%)
Frame = +1
Query: 286 GKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNV-YPSGTVCLSLLDEEKDWRP 462
G +GTP+EGG + + + DYP PPK F+ ++HPNV +G +CL +L ++ W P
Sbjct: 42 GPEGTPYEGGYFVVDIEIPIDYPFRPPKMNFDTKIYHPNVSSQTGAICLDILKDQ--WSP 99
Query: 463 AITIKQILLGIQDLL 507
T+K L+ +Q LL
Sbjct: 100 VYTMKSALISLQSLL 114
>SPCC777.10c |ubc12||ubiquitin conjugating enzyme
Ubc12|Schizosaccharomyces pombe|chr 3|||Manual
Length = 177
Score = 66.9 bits (156), Expect = 3e-12
Identities = 28/70 (40%), Positives = 44/70 (62%)
Frame = +1
Query: 304 WEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLLDEEKDWRPAITIKQI 483
++GG +K R+ D+YP PPK K ++HPN+ G VCL++L +DW P + + I
Sbjct: 65 YKGGKFKFRIQIDDNYPHDPPKVKCLNKIYHPNIDIEGNVCLNIL--RQDWNPVLNLNSI 122
Query: 484 LLGIQDLLMS 513
L+G+Q L +S
Sbjct: 123 LVGLQFLFLS 132
>SPAC1250.03 |ubc14||ubiquitin conjugating enzyme
Ubc14|Schizosaccharomyces pombe|chr 1|||Manual
Length = 155
Score = 61.3 bits (142), Expect = 1e-10
Identities = 28/84 (33%), Positives = 46/84 (54%)
Frame = +1
Query: 256 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSL 435
NL W C G + + GG + + F DYP PP +F ++HPN G VCL++
Sbjct: 35 NLFHWACTALGPSDSVYAGGKFHFSLKFPLDYPFQPPTIEFTTRIYHPNFDSEGNVCLAI 94
Query: 436 LDEEKDWRPAITIKQILLGIQDLL 507
L +++ ++P+I ++ +L I LL
Sbjct: 95 L-KQQVFKPSIKLRSVLEQILQLL 117
>SPBC1198.09 |ubc16||ubiquitin conjugating enzyme
Ubc16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 160
Score = 60.9 bits (141), Expect = 2e-10
Identities = 29/86 (33%), Positives = 48/86 (55%), Gaps = 1/86 (1%)
Frame = +1
Query: 253 MNLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNV-YPSGTVCL 429
+++ W+ I G TP+EGG + L + + YP SPP F+ + HPN+ + +G VC+
Sbjct: 32 VDMFHWKAVIEGPTETPYEGGQWVLDIHVHEGYPISPPSVYFQTKIVHPNISWTNGEVCM 91
Query: 430 SLLDEEKDWRPAITIKQILLGIQDLL 507
+L + W PA +++ L I LL
Sbjct: 92 DIL--KTHWSPAWSLQSACLAIISLL 115
>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 227
Score = 56.0 bits (129), Expect = 5e-09
Identities = 29/87 (33%), Positives = 41/87 (47%), Gaps = 1/87 (1%)
Frame = +1
Query: 256 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSL 435
N++ W I G TP+EGG Y +IF DYP PP + P + +CLS
Sbjct: 33 NILEWHYIITGPPDTPYEGGQYHGTLIFPPDYPFKPPAIRMITP--SGRFQTNTRLCLSF 90
Query: 436 LD-EEKDWRPAITIKQILLGIQDLLMS 513
D K W P+ + IL+G+ + S
Sbjct: 91 SDFHPKSWNPSWMVSTILVGLVSFMTS 117
>SPBC211.07c |ubc8||ubiquitin conjugating enzyme
Ubc8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 47.6 bits (108), Expect = 2e-06
Identities = 22/70 (31%), Positives = 37/70 (52%), Gaps = 1/70 (1%)
Frame = +1
Query: 256 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYP-SGTVCLS 432
N+ + G TP+ GG++K+ + +YP P F +FHPN+ SG+VCL
Sbjct: 28 NMQEFYVRFHGPSETPYSGGIWKVHVELPSEYPWKSPSIGFVNRIFHPNIDELSGSVCLD 87
Query: 433 LLDEEKDWRP 462
++++ W P
Sbjct: 88 VINQ--TWSP 95
>SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme
Mms2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 139
Score = 32.7 bits (71), Expect = 0.058
Identities = 21/88 (23%), Positives = 40/88 (45%), Gaps = 1/88 (1%)
Frame = +1
Query: 253 MNLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNV-YPSGTVCL 429
+ L W I G + E +Y L++ +YP +PP F + P V +G V
Sbjct: 36 ITLSDWNATILGPAHSVHENRIYSLKIHCDANYPDAPPIVTFVSRINLPGVDGETGKVNP 95
Query: 430 SLLDEEKDWRPAITIKQILLGIQDLLMS 513
+D + W+ +++ +LL ++ + S
Sbjct: 96 HKIDCLRHWKREYSMETVLLDLKKEMAS 123
>SPCC1682.11c |||DUF580 family protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 574
Score = 27.9 bits (59), Expect = 1.7
Identities = 12/36 (33%), Positives = 22/36 (61%)
Frame = -1
Query: 207 WSLRQAFRSSAKRALAIPDIFYKNNSIYEPSFNQGL 100
W LR+ +S++ + ++F K NSIY +F++ L
Sbjct: 71 WQLREQEQSNSHISRNENELFSKENSIYNGNFSENL 106
>SPBC1861.02 |abp2||ARS binding protein Abp2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 527
Score = 27.1 bits (57), Expect = 2.9
Identities = 18/60 (30%), Positives = 30/60 (50%)
Frame = -3
Query: 349 NHL*RSYVVYTAHLPMESPFYQVSHIPKSLNS*TIWILHRPRDKTKGMVLTPSFPFFS*T 170
N + RS + A LPME+ +SH ++++ + P +T + TP+F F S T
Sbjct: 370 NTMLRSAFSHVARLPMETVSQLLSHYTETISQ------YLPPSETSPIPKTPNFKFISST 423
>SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 509
Score = 27.1 bits (57), Expect = 2.9
Identities = 13/38 (34%), Positives = 19/38 (50%)
Frame = -2
Query: 377 NLHFGGLDG*SSLKIIRSLYSPPSHGVPFLPGIAHSQV 264
N F G+ L + S SP HG P +PG+ H+ +
Sbjct: 442 NPQFSGVLNLPGLGTMPSFPSPYQHGQPQIPGMLHASL 479
>SPBC16G5.15c |fkh2||fork head transcription factor Fkh2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 642
Score = 25.4 bits (53), Expect = 8.9
Identities = 8/20 (40%), Positives = 13/20 (65%)
Frame = +1
Query: 265 TWECAIPGKKGTPWEGGLYK 324
TW C++ G+ G +G L+K
Sbjct: 142 TWNCSVYGRNGIKVDGKLFK 161
>SPCP1E11.05c |||sterol O-acyltransferase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 472
Score = 25.4 bits (53), Expect = 8.9
Identities = 11/24 (45%), Positives = 13/24 (54%)
Frame = -2
Query: 149 FSTKITRFMSRASIKDYS*CWAID 78
FS +ITRF R D+ CW D
Sbjct: 343 FSAEITRFADRNFYDDWWNCWTWD 366
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,162,520
Number of Sequences: 5004
Number of extensions: 68797
Number of successful extensions: 186
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 172
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 183
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 363302114
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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