SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00452
         (675 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC2C4.16c |rps801|rps8-1|40S ribosomal protein S8|Schizosaccha...   123   3e-29
SPAC521.05 |rps802|rps8-2|40S ribosomal protein S8|Schizosacchar...   123   3e-29
SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||...    28   1.1  
SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki...    28   1.1  
SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|...    28   1.4  
SPCC132.01c ||SPCC1322.17c|DUF814 family protein|Schizosaccharom...    27   1.9  
SPAC139.03 |||transcription factor, zf-fungal binuclear cluster ...    26   5.7  
SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosacc...    26   5.7  
SPAC6G9.02c |nop9||RNA-binding protein Nop9|Schizosaccharomyces ...    25   10.0 
SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit ...    25   10.0 
SPAC31A2.07c |dbp10||ATP-dependent RNA helicase Dbp10 |Schizosac...    25   10.0 

>SPAC2C4.16c |rps801|rps8-1|40S ribosomal protein
           S8|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 200

 Score =  123 bits (296), Expect = 3e-29
 Identities = 56/81 (69%), Positives = 67/81 (82%)
 Frame = +1

Query: 19  MGISRDHWHKRRATGGKRAPIRKKRKYELGRPAANTRLGPQRIHSVRSRGGNTKYRALRL 198
           MGI+RD  HKR ATG KRA  RKKRK+ELGR  +NTR+GP+RIH VR RGGN K+RALRL
Sbjct: 1   MGITRDSRHKRSATGAKRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRL 60

Query: 199 DTGNFSWGSECSTRKTRIMML 261
           D+GNFSWGSE  ++KTRI+ +
Sbjct: 61  DSGNFSWGSEGVSKKTRIIQV 81



 Score = 75.8 bits (178), Expect = 5e-15
 Identities = 42/89 (47%), Positives = 53/89 (59%)
 Frame = +3

Query: 258 VVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESHYTLPLGRKKGAKLTEAEEAIINKK 437
           V Y+ SNNELVRT TL K+AIV +DA PFR WYE+HY + +G  KG K T          
Sbjct: 81  VAYHPSNNELVRTNTLTKSAIVQIDAAPFRVWYETHYGILMG-SKGKKATSTP-----NP 134

Query: 438 RSQKTARKYLARQRLAKVEGALEDNSTRG 524
           +S+   RK+ AR   +KV+ ALE     G
Sbjct: 135 KSKHVQRKHSARLGDSKVDSALETQFAAG 163



 Score = 62.9 bits (146), Expect = 4e-11
 Identities = 28/42 (66%), Positives = 33/42 (78%)
 Frame = +2

Query: 509 QFHTGRLLACVASRPGQCGRADGYILEGKELEFYLRKIKSKR 634
           QF  GRL A V+SRPGQ GR DGYILEG+EL FYLR++  K+
Sbjct: 159 QFAAGRLYAVVSSRPGQSGRCDGYILEGEELHFYLRRMAPKK 200


>SPAC521.05 |rps802|rps8-2|40S ribosomal protein
           S8|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 200

 Score =  123 bits (296), Expect = 3e-29
 Identities = 56/81 (69%), Positives = 67/81 (82%)
 Frame = +1

Query: 19  MGISRDHWHKRRATGGKRAPIRKKRKYELGRPAANTRLGPQRIHSVRSRGGNTKYRALRL 198
           MGI+RD  HKR ATG KRA  RKKRK+ELGR  +NTR+GP+RIH VR RGGN K+RALRL
Sbjct: 1   MGITRDSRHKRSATGAKRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRL 60

Query: 199 DTGNFSWGSECSTRKTRIMML 261
           D+GNFSWGSE  ++KTRI+ +
Sbjct: 61  DSGNFSWGSEGVSKKTRIIQV 81



 Score = 74.9 bits (176), Expect = 9e-15
 Identities = 42/89 (47%), Positives = 53/89 (59%)
 Frame = +3

Query: 258 VVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESHYTLPLGRKKGAKLTEAEEAIINKK 437
           V Y+ SNNELVRT TL K+AIV +DA PFR WYE+HY + +G  KG K T          
Sbjct: 81  VAYHPSNNELVRTNTLTKSAIVQIDAAPFRVWYETHYGILMG-SKGKKATATP-----TP 134

Query: 438 RSQKTARKYLARQRLAKVEGALEDNSTRG 524
           +S+   RK+ AR   +KV+ ALE     G
Sbjct: 135 KSKHVQRKHSARLGDSKVDSALETQFAAG 163



 Score = 62.9 bits (146), Expect = 4e-11
 Identities = 28/42 (66%), Positives = 33/42 (78%)
 Frame = +2

Query: 509 QFHTGRLLACVASRPGQCGRADGYILEGKELEFYLRKIKSKR 634
           QF  GRL A V+SRPGQ GR DGYILEG+EL FYLR++  K+
Sbjct: 159 QFAAGRLYAVVSSRPGQSGRCDGYILEGEELHFYLRRMAPKK 200


>SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 3227

 Score = 28.3 bits (60), Expect = 1.1
 Identities = 10/24 (41%), Positives = 16/24 (66%)
 Frame = +3

Query: 213  LLGIGMFNSQNPYHDVVYNASNNE 284
            L+ I   N +NPY+D++ N S N+
Sbjct: 2386 LIFISQHNGKNPYYDLIVNISENK 2409


>SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase kinase
            Win1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1436

 Score = 28.3 bits (60), Expect = 1.1
 Identities = 22/76 (28%), Positives = 33/76 (43%), Gaps = 2/76 (2%)
 Frame = +1

Query: 31   RDHWHKRRATGGKRAPIRKKRKY--ELGRPAANTRLGPQRIHSVRSRGGNTKYRALRLDT 204
            RD  +K +  G       K+ +   EL    +N  +  Q+   + S    T YRA+ LDT
Sbjct: 1083 RDRLYKSQLIGRVLDDTTKENRLLKELASSKSNITIRWQQGGLIGSGSFGTVYRAVNLDT 1142

Query: 205  GNFSWGSECSTRKTRI 252
            G+     E +  K RI
Sbjct: 1143 GDLMAVKEVALHKPRI 1158


>SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 207

 Score = 27.9 bits (59), Expect = 1.4
 Identities = 17/54 (31%), Positives = 26/54 (48%)
 Frame = +1

Query: 34  DHWHKRRATGGKRAPIRKKRKYELGRPAANTRLGPQRIHSVRSRGGNTKYRALR 195
           + W K   T  K+    +KRK  L R A   RL  + + S+ S+GG    +A +
Sbjct: 32  EKWSKGVKTN-KKEQEAEKRKAALERKAERERLEKEEMESLPSKGGKGSKKAAK 84


>SPCC132.01c ||SPCC1322.17c|DUF814 family
           protein|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1021

 Score = 27.5 bits (58), Expect = 1.9
 Identities = 15/59 (25%), Positives = 28/59 (47%)
 Frame = +3

Query: 333 ATPFRQWYESHYTLPLGRKKGAKLTEAEEAIINKKRSQKTARKYLARQRLAKVEGALED 509
           +TP     + H       KKG+K+  A++    ++R  + AR+  A +   K   ++ED
Sbjct: 754 STPDTTGSDIHIVSEKRGKKGSKVITAKKVSAKERREARRARRQTALEESLKAPISIED 812


>SPAC139.03 |||transcription factor, zf-fungal binuclear cluster
           type |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 625

 Score = 25.8 bits (54), Expect = 5.7
 Identities = 15/39 (38%), Positives = 19/39 (48%)
 Frame = +1

Query: 7   DPTKMGISRDHWHKRRATGGKRAPIRKKRKYELGRPAAN 123
           + TK G  +     RRA     A  RKK K + GRP +N
Sbjct: 3   ETTKSGSKKSGQTSRRAIHSCLACRRKKLKCDHGRPCSN 41


>SPBC16H5.10c |prp43||ATP-dependent RNA helicase
           Prp43|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 735

 Score = 25.8 bits (54), Expect = 5.7
 Identities = 11/25 (44%), Positives = 15/25 (60%)
 Frame = -3

Query: 355 YHCLNGVASTTTIAFLTRVFVRTNS 281
           ++C N V S T +  +  VFVR NS
Sbjct: 518 FYCSNEVLSLTALLSVPNVFVRPNS 542


>SPAC6G9.02c |nop9||RNA-binding protein Nop9|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 655

 Score = 25.0 bits (52), Expect = 10.0
 Identities = 11/29 (37%), Positives = 15/29 (51%)
 Frame = -2

Query: 611 DRTRVLCLLRCNHRRDHTDLGDSPRKPAN 525
           D   + CLLR  H+RD  +  +  RK  N
Sbjct: 434 DANIIPCLLRSKHKRDKANPENKERKLVN 462


>SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit
           Bgs2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1894

 Score = 25.0 bits (52), Expect = 10.0
 Identities = 11/32 (34%), Positives = 16/32 (50%)
 Frame = +2

Query: 125 PGSVLSVSTPFVHVVEILSTVRCVWTPVTSLG 220
           P  VLS   P++  +  L +  C+W P   LG
Sbjct: 682 PILVLSTMKPYLCNITFLGSHLCIWQPKILLG 713


>SPAC31A2.07c |dbp10||ATP-dependent RNA helicase Dbp10
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 848

 Score = 25.0 bits (52), Expect = 10.0
 Identities = 8/23 (34%), Positives = 14/23 (60%)
 Frame = +1

Query: 34  DHWHKRRATGGKRAPIRKKRKYE 102
           D W   +A G   +PIR+ ++Y+
Sbjct: 758 DEWKASKAFGANDSPIRENKRYK 780


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,672,662
Number of Sequences: 5004
Number of extensions: 53574
Number of successful extensions: 168
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 158
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 166
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 309878492
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -