BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00451 (800 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 143 3e-35 SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 134 1e-32 SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 131 1e-31 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 120 2e-28 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 120 2e-28 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 68 1e-12 SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 64 3e-11 SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 52 1e-07 SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual 27 3.1 SPAC637.08 |||iron-sulfur cluster assembly ATPase Nbp35|Schizosa... 26 7.2 SPAC22H12.02 |tfg3|taf14|transcription factor TFIIF complex subu... 26 7.2 SPBC27.03 |meu25||sequence orphan|Schizosaccharomyces pombe|chr ... 25 9.5 SPAC16C9.06c |upf1||ATP-dependent RNA helicase Upf1|Schizosaccha... 25 9.5 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 143 bits (346), Expect = 3e-35 Identities = 67/81 (82%), Positives = 75/81 (92%) Frame = +3 Query: 531 KVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTISGLNVMR 710 K F PEEISAM+L+KMK+TAEAYLGK VTHAVVTVPAYFNDAQRQATKDAGTI+GLNV+R Sbjct: 143 KKFTPEEISAMILSKMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVIR 202 Query: 711 IINEPTAAAIAYGLDKKEERR 773 I+NEPTAAAIAYGLDK + + Sbjct: 203 IVNEPTAAAIAYGLDKTDTEK 223 Score = 124 bits (298), Expect = 2e-29 Identities = 56/84 (66%), Positives = 71/84 (84%) Frame = +1 Query: 259 KNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPENTVFDAKRLIGREWSD 438 KNGRVEIIANDQGNRITPSYVAFT+D ERL+G+AAKNQ +NPENT+FD KRLIGR++ + Sbjct: 53 KNGRVEIIANDQGNRITPSYVAFTED-ERLVGEAAKNQAPSNPENTIFDIKRLIGRKFDE 111 Query: 439 QTVQHDVKFFPFKVVEKNSKPHVQ 510 +T+ D+K FPF +V ++P V+ Sbjct: 112 KTMAKDIKSFPFHIVNDKNRPLVE 135 Score = 37.5 bits (83), Expect = 0.002 Identities = 16/19 (84%), Positives = 16/19 (84%) Frame = +2 Query: 206 GTVIGIDLGTTYSCVGATK 262 GTVIGIDLGTTYSCV K Sbjct: 35 GTVIGIDLGTTYSCVAVMK 53 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 134 bits (325), Expect = 1e-32 Identities = 66/94 (70%), Positives = 76/94 (80%) Frame = +3 Query: 486 KEQQTSCSVQTSQGDKVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQ 665 K+ + V+ K F PEEIS+MVL KM+ETAEAYLG KVT AVVTVPAYFND+QRQ Sbjct: 95 KDGKPVLQVEYKGETKTFTPEEISSMVLMKMRETAEAYLGGKVTDAVVTVPAYFNDSQRQ 154 Query: 666 ATKDAGTISGLNVMRIINEPTAAAIAYGLDKKEE 767 ATKDAG I+GLNV+RIINEPTAAAIAYGLD+ + Sbjct: 155 ATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQ 188 Score = 127 bits (306), Expect = 2e-30 Identities = 61/93 (65%), Positives = 72/93 (77%) Frame = +1 Query: 256 YKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPENTVFDAKRLIGREWS 435 + N RVEIIANDQGNR TPSYVAFT D ERLIGDAAKNQ+ NP NT+FDAKRLIGR++ Sbjct: 19 FSNNRVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAMNPHNTIFDAKRLIGRKFD 77 Query: 436 DQTVQHDVKFFPFKVVEKNSKPHVQYKLHKATK 534 D VQ D+K +PFKV+ K+ KP +Q + TK Sbjct: 78 DPEVQSDMKHWPFKVISKDGKPVLQVEYKGETK 110 Score = 32.3 bits (70), Expect = 0.083 Identities = 13/13 (100%), Positives = 13/13 (100%) Frame = +2 Query: 215 IGIDLGTTYSCVG 253 IGIDLGTTYSCVG Sbjct: 5 IGIDLGTTYSCVG 17 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 131 bits (317), Expect = 1e-31 Identities = 64/94 (68%), Positives = 76/94 (80%) Frame = +3 Query: 486 KEQQTSCSVQTSQGDKVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQ 665 K+ + V+ K F PEEIS+MVL KM+E+AEA+LG KVT AVVTVPAYFND+QRQ Sbjct: 95 KDGKPLIQVEFKGETKTFTPEEISSMVLLKMRESAEAFLGGKVTDAVVTVPAYFNDSQRQ 154 Query: 666 ATKDAGTISGLNVMRIINEPTAAAIAYGLDKKEE 767 ATKDAG I+GLNV+RIINEPTAAAIAYGLD+ + Sbjct: 155 ATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQ 188 Score = 130 bits (314), Expect = 2e-31 Identities = 62/93 (66%), Positives = 73/93 (78%) Frame = +1 Query: 256 YKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPENTVFDAKRLIGREWS 435 + N RVEIIANDQGNR TPSYVAFT D ERLIGDAAKNQ+ NP NT+FDAKRLIGR ++ Sbjct: 19 FSNNRVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAMNPHNTIFDAKRLIGRRFN 77 Query: 436 DQTVQHDVKFFPFKVVEKNSKPHVQYKLHKATK 534 D VQ D+K +PFKV+EK+ KP +Q + TK Sbjct: 78 DPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETK 110 Score = 32.3 bits (70), Expect = 0.083 Identities = 13/13 (100%), Positives = 13/13 (100%) Frame = +2 Query: 215 IGIDLGTTYSCVG 253 IGIDLGTTYSCVG Sbjct: 5 IGIDLGTTYSCVG 17 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 120 bits (290), Expect = 2e-28 Identities = 53/79 (67%), Positives = 67/79 (84%) Frame = +3 Query: 531 KVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTISGLNVMR 710 K ++P +I +L+KM+ETA YLGK V +AVVTVPAYFND+QRQATK AG I+GLNV+R Sbjct: 156 KTYSPSQIGGFILSKMRETASTYLGKDVKNAVVTVPAYFNDSQRQATKAAGAIAGLNVLR 215 Query: 711 IINEPTAAAIAYGLDKKEE 767 ++NEPTAAA+AYGLDKK + Sbjct: 216 VVNEPTAAALAYGLDKKND 234 Score = 91.9 bits (218), Expect = 1e-19 Identities = 43/84 (51%), Positives = 57/84 (67%), Gaps = 1/84 (1%) Frame = +1 Query: 247 CRCYKNGRV-EIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPENTVFDAKRLIG 423 C G+ ++IAN +G R TPS VAFT+DGERL+G +AK Q NPENT F KRLIG Sbjct: 63 CLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVINPENTFFATKRLIG 122 Query: 424 REWSDQTVQHDVKFFPFKVVEKNS 495 R + + VQ D+K P+K+VE ++ Sbjct: 123 RRFKEPEVQRDIKEVPYKIVEHSN 146 Score = 28.7 bits (61), Expect = 1.0 Identities = 12/15 (80%), Positives = 13/15 (86%) Frame = +2 Query: 206 GTVIGIDLGTTYSCV 250 G VIGIDLGTT SC+ Sbjct: 50 GPVIGIDLGTTTSCL 64 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 120 bits (290), Expect = 2e-28 Identities = 58/80 (72%), Positives = 68/80 (85%) Frame = +3 Query: 531 KVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTISGLNVMR 710 K F +EISAMVLTKMKE +EA L K+V AV+TVPAYF+D+QR ATKDAG I+GLNV+R Sbjct: 114 KQFTAQEISAMVLTKMKEISEAKLNKRVEKAVITVPAYFSDSQRAATKDAGAIAGLNVLR 173 Query: 711 IINEPTAAAIAYGLDKKEER 770 IINEPTAAAIAYGLD K ++ Sbjct: 174 IINEPTAAAIAYGLDAKSDK 193 Score = 108 bits (260), Expect = 8e-25 Identities = 50/85 (58%), Positives = 62/85 (72%) Frame = +1 Query: 256 YKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPENTVFDAKRLIGREWS 435 ++ VEII NDQG R TPS+VAFT+ ERL+GDAAKNQ NP NTVFDAKRLIGR + Sbjct: 23 WETANVEIIPNDQGARTTPSFVAFTET-ERLVGDAAKNQAAMNPRNTVFDAKRLIGRRYE 81 Query: 436 DQTVQHDVKFFPFKVVEKNSKPHVQ 510 D Q D+K +PFKV++ N P ++ Sbjct: 82 DPETQKDIKHWPFKVIDNNGIPTIE 106 Score = 29.9 bits (64), Expect = 0.44 Identities = 12/12 (100%), Positives = 12/12 (100%) Frame = +2 Query: 215 IGIDLGTTYSCV 250 IGIDLGTTYSCV Sbjct: 9 IGIDLGTTYSCV 20 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 68.1 bits (159), Expect = 1e-12 Identities = 33/85 (38%), Positives = 53/85 (62%) Frame = +3 Query: 510 VQTSQGDKVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTI 689 VQ + F+ ++ A TK+K AEA L V+ V+++PA+F D QR+A +A I Sbjct: 107 VQYLNEETAFSNIQLIAAYFTKIKAIAEAELIGSVSDVVISIPAWFTDIQRRALLEAANI 166 Query: 690 SGLNVMRIINEPTAAAIAYGLDKKE 764 +GLN +R++N+ AAA+ YG+ K + Sbjct: 167 AGLNPLRLMNDNAAAALTYGITKTD 191 Score = 51.6 bits (118), Expect = 1e-07 Identities = 29/88 (32%), Positives = 47/88 (53%), Gaps = 1/88 (1%) Frame = +1 Query: 226 FGYHLLMCRCYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPENTVFD 405 FG + +N +++I N+ NR TPS V++ + R +G+AAK+ +N NTV Sbjct: 12 FGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERS-RFLGEAAKSAEASNFRNTVGS 70 Query: 406 AKRLIGREWSDQTVQH-DVKFFPFKVVE 486 KRL GR + D ++ + F K+ E Sbjct: 71 LKRLAGRTYDDPEIKDIESNFISAKLTE 98 >SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 63.7 bits (148), Expect = 3e-11 Identities = 29/72 (40%), Positives = 46/72 (63%) Frame = +3 Query: 528 DKVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTISGLNVM 707 +K+ E S L ++ E+AE +LG KV V++VP YF DAQR+A + A +GL V+ Sbjct: 135 EKILTAHEASVRHLRRLTESAEDFLGTKVNGCVMSVPVYFTDAQRKALESAANEAGLPVL 194 Query: 708 RIINEPTAAAIA 743 ++I++P A +A Sbjct: 195 QLIHDPAAVILA 206 Score = 47.2 bits (107), Expect = 3e-06 Identities = 21/65 (32%), Positives = 41/65 (63%) Frame = +1 Query: 259 KNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPENTVFDAKRLIGREWSD 438 ++G+ +++AN++GNR PS +++ D E G A+ QL N +N+V + + L+G+ + Sbjct: 42 RDGKTDVLANEEGNRQIPSILSYHGDQE-YHGVQARGQLVRNADNSVTNFRDLLGKSHDE 100 Query: 439 QTVQH 453 T+ H Sbjct: 101 LTLHH 105 >SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 51.6 bits (118), Expect = 1e-07 Identities = 29/90 (32%), Positives = 47/90 (52%) Frame = +3 Query: 489 EQQTSCSVQTSQGDKVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQA 668 E+ TS ++ ++ EEI AM + AE +K+T V+TVP +FN+ QR Sbjct: 119 EETTSGISFVISDEENYSLEEIIAMTMEHYISLAEEMAHEKITDLVLTVPPHFNELQRSI 178 Query: 669 TKDAGTISGLNVMRIINEPTAAAIAYGLDK 758 +A I +V+ +I++ A AI Y L + Sbjct: 179 LLEAARILNKHVLALIDDNVAVAIEYSLSR 208 >SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual Length = 4717 Score = 27.1 bits (57), Expect = 3.1 Identities = 12/41 (29%), Positives = 21/41 (51%) Frame = -1 Query: 680 SIFGCLTLSIIEISRHSNNSMSNLFSKVSLSSLLHFSQNHS 558 +I G + L+ I ++N +N+F L+ L HF + S Sbjct: 2586 NILGRIALAFTSIDENANLESANIFEHARLALLQHFMDHSS 2626 >SPAC637.08 |||iron-sulfur cluster assembly ATPase Nbp35|Schizosaccharomyces pombe|chr 1|||Manual Length = 317 Score = 25.8 bits (54), Expect = 7.2 Identities = 10/24 (41%), Positives = 12/24 (50%) Frame = -2 Query: 550 SSGANTLSPCEVCTEHEVCCSFQR 479 S A T S CE C ++C S R Sbjct: 20 SENAGTASACEGCPNQQICASAPR 43 >SPAC22H12.02 |tfg3|taf14|transcription factor TFIIF complex subunit Tfg3|Schizosaccharomyces pombe|chr 1|||Manual Length = 241 Score = 25.8 bits (54), Expect = 7.2 Identities = 15/39 (38%), Positives = 24/39 (61%), Gaps = 2/39 (5%) Frame = -3 Query: 549 LQEQILCRLVKFVLNMRFAVLF--NDLEGEELNIMLYSL 439 LQE L ++V+ V + ++ ND+EG E +I LY+L Sbjct: 185 LQEDDLLQVVQMVNENKTPDMYVRNDIEGGEFHIDLYTL 223 >SPBC27.03 |meu25||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 622 Score = 25.4 bits (53), Expect = 9.5 Identities = 9/20 (45%), Positives = 13/20 (65%) Frame = +1 Query: 337 GERLIGDAAKNQLTTNPENT 396 GE ++ D+ N TNP+NT Sbjct: 6 GENIVSDSIVNMELTNPDNT 25 >SPAC16C9.06c |upf1||ATP-dependent RNA helicase Upf1|Schizosaccharomyces pombe|chr 1|||Manual Length = 925 Score = 25.4 bits (53), Expect = 9.5 Identities = 9/14 (64%), Positives = 11/14 (78%) Frame = -1 Query: 329 VKATYEGVMRFPWS 288 +K TYEG +R PWS Sbjct: 284 MKLTYEGELRAPWS 297 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,080,294 Number of Sequences: 5004 Number of extensions: 60242 Number of successful extensions: 199 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 178 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 195 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 388424860 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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