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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00451
         (800 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M...   143   3e-35
SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p...   134   1e-32
SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ...   131   1e-31
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce...   120   2e-28
SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc...   120   2e-28
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch...    68   1e-12
SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom...    64   3e-11
SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe...    52   1e-07
SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual        27   3.1  
SPAC637.08 |||iron-sulfur cluster assembly ATPase Nbp35|Schizosa...    26   7.2  
SPAC22H12.02 |tfg3|taf14|transcription factor TFIIF complex subu...    26   7.2  
SPBC27.03 |meu25||sequence orphan|Schizosaccharomyces pombe|chr ...    25   9.5  
SPAC16C9.06c |upf1||ATP-dependent RNA helicase Upf1|Schizosaccha...    25   9.5  

>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 663

 Score =  143 bits (346), Expect = 3e-35
 Identities = 67/81 (82%), Positives = 75/81 (92%)
 Frame = +3

Query: 531 KVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTISGLNVMR 710
           K F PEEISAM+L+KMK+TAEAYLGK VTHAVVTVPAYFNDAQRQATKDAGTI+GLNV+R
Sbjct: 143 KKFTPEEISAMILSKMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVIR 202

Query: 711 IINEPTAAAIAYGLDKKEERR 773
           I+NEPTAAAIAYGLDK +  +
Sbjct: 203 IVNEPTAAAIAYGLDKTDTEK 223



 Score =  124 bits (298), Expect = 2e-29
 Identities = 56/84 (66%), Positives = 71/84 (84%)
 Frame = +1

Query: 259 KNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPENTVFDAKRLIGREWSD 438
           KNGRVEIIANDQGNRITPSYVAFT+D ERL+G+AAKNQ  +NPENT+FD KRLIGR++ +
Sbjct: 53  KNGRVEIIANDQGNRITPSYVAFTED-ERLVGEAAKNQAPSNPENTIFDIKRLIGRKFDE 111

Query: 439 QTVQHDVKFFPFKVVEKNSKPHVQ 510
           +T+  D+K FPF +V   ++P V+
Sbjct: 112 KTMAKDIKSFPFHIVNDKNRPLVE 135



 Score = 37.5 bits (83), Expect = 0.002
 Identities = 16/19 (84%), Positives = 16/19 (84%)
 Frame = +2

Query: 206 GTVIGIDLGTTYSCVGATK 262
           GTVIGIDLGTTYSCV   K
Sbjct: 35  GTVIGIDLGTTYSCVAVMK 53


>SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 647

 Score =  134 bits (325), Expect = 1e-32
 Identities = 66/94 (70%), Positives = 76/94 (80%)
 Frame = +3

Query: 486 KEQQTSCSVQTSQGDKVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQ 665
           K+ +    V+     K F PEEIS+MVL KM+ETAEAYLG KVT AVVTVPAYFND+QRQ
Sbjct: 95  KDGKPVLQVEYKGETKTFTPEEISSMVLMKMRETAEAYLGGKVTDAVVTVPAYFNDSQRQ 154

Query: 666 ATKDAGTISGLNVMRIINEPTAAAIAYGLDKKEE 767
           ATKDAG I+GLNV+RIINEPTAAAIAYGLD+  +
Sbjct: 155 ATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQ 188



 Score =  127 bits (306), Expect = 2e-30
 Identities = 61/93 (65%), Positives = 72/93 (77%)
 Frame = +1

Query: 256 YKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPENTVFDAKRLIGREWS 435
           + N RVEIIANDQGNR TPSYVAFT D ERLIGDAAKNQ+  NP NT+FDAKRLIGR++ 
Sbjct: 19  FSNNRVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAMNPHNTIFDAKRLIGRKFD 77

Query: 436 DQTVQHDVKFFPFKVVEKNSKPHVQYKLHKATK 534
           D  VQ D+K +PFKV+ K+ KP +Q +    TK
Sbjct: 78  DPEVQSDMKHWPFKVISKDGKPVLQVEYKGETK 110



 Score = 32.3 bits (70), Expect = 0.083
 Identities = 13/13 (100%), Positives = 13/13 (100%)
 Frame = +2

Query: 215 IGIDLGTTYSCVG 253
           IGIDLGTTYSCVG
Sbjct: 5   IGIDLGTTYSCVG 17


>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 644

 Score =  131 bits (317), Expect = 1e-31
 Identities = 64/94 (68%), Positives = 76/94 (80%)
 Frame = +3

Query: 486 KEQQTSCSVQTSQGDKVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQ 665
           K+ +    V+     K F PEEIS+MVL KM+E+AEA+LG KVT AVVTVPAYFND+QRQ
Sbjct: 95  KDGKPLIQVEFKGETKTFTPEEISSMVLLKMRESAEAFLGGKVTDAVVTVPAYFNDSQRQ 154

Query: 666 ATKDAGTISGLNVMRIINEPTAAAIAYGLDKKEE 767
           ATKDAG I+GLNV+RIINEPTAAAIAYGLD+  +
Sbjct: 155 ATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQ 188



 Score =  130 bits (314), Expect = 2e-31
 Identities = 62/93 (66%), Positives = 73/93 (78%)
 Frame = +1

Query: 256 YKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPENTVFDAKRLIGREWS 435
           + N RVEIIANDQGNR TPSYVAFT D ERLIGDAAKNQ+  NP NT+FDAKRLIGR ++
Sbjct: 19  FSNNRVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAMNPHNTIFDAKRLIGRRFN 77

Query: 436 DQTVQHDVKFFPFKVVEKNSKPHVQYKLHKATK 534
           D  VQ D+K +PFKV+EK+ KP +Q +    TK
Sbjct: 78  DPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETK 110



 Score = 32.3 bits (70), Expect = 0.083
 Identities = 13/13 (100%), Positives = 13/13 (100%)
 Frame = +2

Query: 215 IGIDLGTTYSCVG 253
           IGIDLGTTYSCVG
Sbjct: 5   IGIDLGTTYSCVG 17


>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 674

 Score =  120 bits (290), Expect = 2e-28
 Identities = 53/79 (67%), Positives = 67/79 (84%)
 Frame = +3

Query: 531 KVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTISGLNVMR 710
           K ++P +I   +L+KM+ETA  YLGK V +AVVTVPAYFND+QRQATK AG I+GLNV+R
Sbjct: 156 KTYSPSQIGGFILSKMRETASTYLGKDVKNAVVTVPAYFNDSQRQATKAAGAIAGLNVLR 215

Query: 711 IINEPTAAAIAYGLDKKEE 767
           ++NEPTAAA+AYGLDKK +
Sbjct: 216 VVNEPTAAALAYGLDKKND 234



 Score = 91.9 bits (218), Expect = 1e-19
 Identities = 43/84 (51%), Positives = 57/84 (67%), Gaps = 1/84 (1%)
 Frame = +1

Query: 247 CRCYKNGRV-EIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPENTVFDAKRLIG 423
           C     G+  ++IAN +G R TPS VAFT+DGERL+G +AK Q   NPENT F  KRLIG
Sbjct: 63  CLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVINPENTFFATKRLIG 122

Query: 424 REWSDQTVQHDVKFFPFKVVEKNS 495
           R + +  VQ D+K  P+K+VE ++
Sbjct: 123 RRFKEPEVQRDIKEVPYKIVEHSN 146



 Score = 28.7 bits (61), Expect = 1.0
 Identities = 12/15 (80%), Positives = 13/15 (86%)
 Frame = +2

Query: 206 GTVIGIDLGTTYSCV 250
           G VIGIDLGTT SC+
Sbjct: 50  GPVIGIDLGTTTSCL 64


>SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 613

 Score =  120 bits (290), Expect = 2e-28
 Identities = 58/80 (72%), Positives = 68/80 (85%)
 Frame = +3

Query: 531 KVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTISGLNVMR 710
           K F  +EISAMVLTKMKE +EA L K+V  AV+TVPAYF+D+QR ATKDAG I+GLNV+R
Sbjct: 114 KQFTAQEISAMVLTKMKEISEAKLNKRVEKAVITVPAYFSDSQRAATKDAGAIAGLNVLR 173

Query: 711 IINEPTAAAIAYGLDKKEER 770
           IINEPTAAAIAYGLD K ++
Sbjct: 174 IINEPTAAAIAYGLDAKSDK 193



 Score =  108 bits (260), Expect = 8e-25
 Identities = 50/85 (58%), Positives = 62/85 (72%)
 Frame = +1

Query: 256 YKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPENTVFDAKRLIGREWS 435
           ++   VEII NDQG R TPS+VAFT+  ERL+GDAAKNQ   NP NTVFDAKRLIGR + 
Sbjct: 23  WETANVEIIPNDQGARTTPSFVAFTET-ERLVGDAAKNQAAMNPRNTVFDAKRLIGRRYE 81

Query: 436 DQTVQHDVKFFPFKVVEKNSKPHVQ 510
           D   Q D+K +PFKV++ N  P ++
Sbjct: 82  DPETQKDIKHWPFKVIDNNGIPTIE 106



 Score = 29.9 bits (64), Expect = 0.44
 Identities = 12/12 (100%), Positives = 12/12 (100%)
 Frame = +2

Query: 215 IGIDLGTTYSCV 250
           IGIDLGTTYSCV
Sbjct: 9   IGIDLGTTYSCV 20


>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
           Pss1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 720

 Score = 68.1 bits (159), Expect = 1e-12
 Identities = 33/85 (38%), Positives = 53/85 (62%)
 Frame = +3

Query: 510 VQTSQGDKVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTI 689
           VQ    +  F+  ++ A   TK+K  AEA L   V+  V+++PA+F D QR+A  +A  I
Sbjct: 107 VQYLNEETAFSNIQLIAAYFTKIKAIAEAELIGSVSDVVISIPAWFTDIQRRALLEAANI 166

Query: 690 SGLNVMRIINEPTAAAIAYGLDKKE 764
           +GLN +R++N+  AAA+ YG+ K +
Sbjct: 167 AGLNPLRLMNDNAAAALTYGITKTD 191



 Score = 51.6 bits (118), Expect = 1e-07
 Identities = 29/88 (32%), Positives = 47/88 (53%), Gaps = 1/88 (1%)
 Frame = +1

Query: 226 FGYHLLMCRCYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPENTVFD 405
           FG    +    +N  +++I N+  NR TPS V++ +   R +G+AAK+   +N  NTV  
Sbjct: 12  FGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERS-RFLGEAAKSAEASNFRNTVGS 70

Query: 406 AKRLIGREWSDQTVQH-DVKFFPFKVVE 486
            KRL GR + D  ++  +  F   K+ E
Sbjct: 71  LKRLAGRTYDDPEIKDIESNFISAKLTE 98


>SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 566

 Score = 63.7 bits (148), Expect = 3e-11
 Identities = 29/72 (40%), Positives = 46/72 (63%)
 Frame = +3

Query: 528 DKVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTISGLNVM 707
           +K+    E S   L ++ E+AE +LG KV   V++VP YF DAQR+A + A   +GL V+
Sbjct: 135 EKILTAHEASVRHLRRLTESAEDFLGTKVNGCVMSVPVYFTDAQRKALESAANEAGLPVL 194

Query: 708 RIINEPTAAAIA 743
           ++I++P A  +A
Sbjct: 195 QLIHDPAAVILA 206



 Score = 47.2 bits (107), Expect = 3e-06
 Identities = 21/65 (32%), Positives = 41/65 (63%)
 Frame = +1

Query: 259 KNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPENTVFDAKRLIGREWSD 438
           ++G+ +++AN++GNR  PS +++  D E   G  A+ QL  N +N+V + + L+G+   +
Sbjct: 42  RDGKTDVLANEEGNRQIPSILSYHGDQE-YHGVQARGQLVRNADNSVTNFRDLLGKSHDE 100

Query: 439 QTVQH 453
            T+ H
Sbjct: 101 LTLHH 105


>SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 848

 Score = 51.6 bits (118), Expect = 1e-07
 Identities = 29/90 (32%), Positives = 47/90 (52%)
 Frame = +3

Query: 489 EQQTSCSVQTSQGDKVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQA 668
           E+ TS        ++ ++ EEI AM +      AE    +K+T  V+TVP +FN+ QR  
Sbjct: 119 EETTSGISFVISDEENYSLEEIIAMTMEHYISLAEEMAHEKITDLVLTVPPHFNELQRSI 178

Query: 669 TKDAGTISGLNVMRIINEPTAAAIAYGLDK 758
             +A  I   +V+ +I++  A AI Y L +
Sbjct: 179 LLEAARILNKHVLALIDDNVAVAIEYSLSR 208


>SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 4717

 Score = 27.1 bits (57), Expect = 3.1
 Identities = 12/41 (29%), Positives = 21/41 (51%)
 Frame = -1

Query: 680  SIFGCLTLSIIEISRHSNNSMSNLFSKVSLSSLLHFSQNHS 558
            +I G + L+   I  ++N   +N+F    L+ L HF  + S
Sbjct: 2586 NILGRIALAFTSIDENANLESANIFEHARLALLQHFMDHSS 2626


>SPAC637.08 |||iron-sulfur cluster assembly ATPase
           Nbp35|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 317

 Score = 25.8 bits (54), Expect = 7.2
 Identities = 10/24 (41%), Positives = 12/24 (50%)
 Frame = -2

Query: 550 SSGANTLSPCEVCTEHEVCCSFQR 479
           S  A T S CE C   ++C S  R
Sbjct: 20  SENAGTASACEGCPNQQICASAPR 43


>SPAC22H12.02 |tfg3|taf14|transcription factor TFIIF complex subunit
           Tfg3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 241

 Score = 25.8 bits (54), Expect = 7.2
 Identities = 15/39 (38%), Positives = 24/39 (61%), Gaps = 2/39 (5%)
 Frame = -3

Query: 549 LQEQILCRLVKFVLNMRFAVLF--NDLEGEELNIMLYSL 439
           LQE  L ++V+ V   +   ++  ND+EG E +I LY+L
Sbjct: 185 LQEDDLLQVVQMVNENKTPDMYVRNDIEGGEFHIDLYTL 223


>SPBC27.03 |meu25||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 622

 Score = 25.4 bits (53), Expect = 9.5
 Identities = 9/20 (45%), Positives = 13/20 (65%)
 Frame = +1

Query: 337 GERLIGDAAKNQLTTNPENT 396
           GE ++ D+  N   TNP+NT
Sbjct: 6   GENIVSDSIVNMELTNPDNT 25


>SPAC16C9.06c |upf1||ATP-dependent RNA helicase
           Upf1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 925

 Score = 25.4 bits (53), Expect = 9.5
 Identities = 9/14 (64%), Positives = 11/14 (78%)
 Frame = -1

Query: 329 VKATYEGVMRFPWS 288
           +K TYEG +R PWS
Sbjct: 284 MKLTYEGELRAPWS 297


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,080,294
Number of Sequences: 5004
Number of extensions: 60242
Number of successful extensions: 199
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 178
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 195
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 388424860
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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