Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00451
(800 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_45647| Best HMM Match : HSP70 (HMM E-Value=0) 166 2e-41
SB_45540| Best HMM Match : HSP70 (HMM E-Value=0) 139 2e-33
SB_8490| Best HMM Match : HSP70 (HMM E-Value=0) 136 3e-32
SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.) 129 3e-30
SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049) 98 6e-21
SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.) 84 1e-16
SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36) 84 1e-16
SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.) 67 2e-11
SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07) 36 0.038
SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16) 36 0.038
SB_8282| Best HMM Match : NHL (HMM E-Value=9.2e-23) 31 0.82
SB_16422| Best HMM Match : HMG_box (HMM E-Value=8.7e-26) 30 1.9
SB_41346| Best HMM Match : Ion_trans (HMM E-Value=0) 29 3.3
SB_23114| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 5.8
SB_42147| Best HMM Match : Thioredoxin (HMM E-Value=0.3) 29 5.8
>SB_45647| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1327
Score = 166 bits (403), Expect = 2e-41
Identities = 75/93 (80%), Positives = 83/93 (89%)
Frame = +1
Query: 256 YKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPENTVFDAKRLIGREWS 435
+KNGRVEIIANDQGNRITPSYVAFT++GERLIGD AKNQLTTNPENTVFDAKRLIGR W
Sbjct: 718 FKNGRVEIIANDQGNRITPSYVAFTEEGERLIGDGAKNQLTTNPENTVFDAKRLIGRSWD 777
Query: 436 DQTVQHDVKFFPFKVVEKNSKPHVQYKLHKATK 534
D +VQ+D+KFFPFKVV+KN KPHVQ K+ K
Sbjct: 778 DTSVQNDLKFFPFKVVDKNKKPHVQVKVKDEIK 810
Score = 149 bits (362), Expect = 2e-36
Identities = 74/93 (79%), Positives = 79/93 (84%)
Frame = +3
Query: 486 KEQQTSCSVQTSQGDKVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQ 665
K ++ V+ K FA EEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQ
Sbjct: 795 KNKKPHVQVKVKDEIKTFAAEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQ 854
Query: 666 ATKDAGTISGLNVMRIINEPTAAAIAYGLDKKE 764
ATKDAG I+GL VMRIINEPTAAAIAYG+DKKE
Sbjct: 855 ATKDAGAIAGLTVMRIINEPTAAAIAYGMDKKE 887
Score = 40.7 bits (91), Expect = 0.001
Identities = 17/20 (85%), Positives = 18/20 (90%)
Frame = +2
Query: 203 IGTVIGIDLGTTYSCVGATK 262
+GTVIGIDLGTTYSCVG K
Sbjct: 700 VGTVIGIDLGTTYSCVGVFK 719
>SB_45540| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1097
Score = 139 bits (337), Expect = 2e-33
Identities = 68/77 (88%), Positives = 73/77 (94%)
Frame = +3
Query: 531 KVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTISGLNVMR 710
K F PEEIS+MVLTKMKETAEAYLG KVT AVVTVPAYF+D+QRQATKDAGTI+GLNV+R
Sbjct: 112 KSFFPEEISSMVLTKMKETAEAYLGAKVTDAVVTVPAYFHDSQRQATKDAGTIAGLNVLR 171
Query: 711 IINEPTAAAIAYGLDKK 761
IINEPTAAAIAYGLDKK
Sbjct: 172 IINEPTAAAIAYGLDKK 188
Score = 117 bits (282), Expect = 1e-26
Identities = 54/85 (63%), Positives = 68/85 (80%)
Frame = +1
Query: 256 YKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPENTVFDAKRLIGREWS 435
+++G+VEIIANDQGNR TPSYVAF+ D ERLIGDAAKNQ+ NPENTVFDAKRLIGR++
Sbjct: 21 FQHGKVEIIANDQGNRTTPSYVAFS-DTERLIGDAAKNQVAMNPENTVFDAKRLIGRKFD 79
Query: 436 DQTVQHDVKFFPFKVVEKNSKPHVQ 510
D V D+ +PF V+ + +P +Q
Sbjct: 80 DPVVARDMTHWPFHVIREGERPKIQ 104
Score = 31.9 bits (69), Expect = 0.62
Identities = 12/13 (92%), Positives = 13/13 (100%)
Frame = +2
Query: 215 IGIDLGTTYSCVG 253
+GIDLGTTYSCVG
Sbjct: 7 VGIDLGTTYSCVG 19
>SB_8490| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 640
Score = 136 bits (328), Expect = 3e-32
Identities = 64/76 (84%), Positives = 72/76 (94%)
Frame = +3
Query: 531 KVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTISGLNVMR 710
K FA EEIS+MVL KMKETAEAYLG+KVT AV+TVPAYFND+QRQATKDAGTI+GLNV+R
Sbjct: 112 KTFAAEEISSMVLGKMKETAEAYLGQKVTSAVITVPAYFNDSQRQATKDAGTIAGLNVLR 171
Query: 711 IINEPTAAAIAYGLDK 758
+INEPTAAA+AYGLDK
Sbjct: 172 VINEPTAAALAYGLDK 187
Score = 131 bits (316), Expect = 7e-31
Identities = 61/94 (64%), Positives = 75/94 (79%), Gaps = 2/94 (2%)
Frame = +1
Query: 256 YKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPENTVFDAKRLIGREWS 435
++NG+VEIIANDQGNR TPSYV FT D ERL+GDAAKNQ+ NPENT+FDAKRLIGR +
Sbjct: 21 FQNGKVEIIANDQGNRTTPSYVGFT-DTERLVGDAAKNQVALNPENTIFDAKRLIGRRYD 79
Query: 436 DQTVQHDVKFFPFKVVEKNSKPHVQ--YKLHKAT 531
D TVQ D+K +PFK++ N+KP +Q YK + T
Sbjct: 80 DATVQSDMKLWPFKIISDNNKPKIQVEYKGERKT 113
Score = 32.3 bits (70), Expect = 0.47
Identities = 13/13 (100%), Positives = 13/13 (100%)
Frame = +2
Query: 215 IGIDLGTTYSCVG 253
IGIDLGTTYSCVG
Sbjct: 7 IGIDLGTTYSCVG 19
>SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 746
Score = 129 bits (311), Expect = 3e-30
Identities = 63/71 (88%), Positives = 67/71 (94%)
Frame = +3
Query: 549 EISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTISGLNVMRIINEPT 728
+IS+MVL KMKETAEAYLG KVT AVVTVPAYFND+QRQATKDAG ISGLNV+RIINEPT
Sbjct: 211 QISSMVLNKMKETAEAYLGCKVTDAVVTVPAYFNDSQRQATKDAGVISGLNVLRIINEPT 270
Query: 729 AAAIAYGLDKK 761
AAAIAYGLDKK
Sbjct: 271 AAAIAYGLDKK 281
Score = 115 bits (277), Expect = 4e-26
Identities = 55/93 (59%), Positives = 71/93 (76%)
Frame = +1
Query: 256 YKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPENTVFDAKRLIGREWS 435
+++G+VEIIANDQGNR TPSYVAFT D ERL+GDAAKNQ+ NP NT+FDAKRLIGR +
Sbjct: 21 FQHGKVEIIANDQGNRTTPSYVAFT-DEERLVGDAAKNQVAMNPTNTIFDAKRLIGRRFD 79
Query: 436 DQTVQHDVKFFPFKVVEKNSKPHVQYKLHKATK 534
D V+ D+K + F+VV + +P V+ + TK
Sbjct: 80 DPGVKDDMKHWSFEVVNEAGRPKVKVEYKGETK 112
Score = 87.8 bits (208), Expect = 9e-18
Identities = 43/51 (84%), Positives = 45/51 (88%)
Frame = +3
Query: 531 KVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAG 683
K F EEIS+MVL KMKETAEAYLG KVT AVVTVPAYFND+QRQATKDAG
Sbjct: 137 KTFFAEEISSMVLNKMKETAEAYLGCKVTDAVVTVPAYFNDSQRQATKDAG 187
Score = 32.3 bits (70), Expect = 0.47
Identities = 13/13 (100%), Positives = 13/13 (100%)
Frame = +2
Query: 215 IGIDLGTTYSCVG 253
IGIDLGTTYSCVG
Sbjct: 7 IGIDLGTTYSCVG 19
>SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049)
Length = 179
Score = 98.3 bits (234), Expect = 6e-21
Identities = 58/108 (53%), Positives = 72/108 (66%), Gaps = 24/108 (22%)
Frame = +3
Query: 507 SVQTSQGDKVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGT 686
+V+ + FAPE+ISA+VL ++K AEA+LG+ V AV+TVPA+FNDAQRQATKDAGT
Sbjct: 3 AVEVCGEKRSFAPEQISAVVLEQLKADAEAFLGQAVKRAVITVPAHFNDAQRQATKDAGT 62
Query: 687 ISGLN------------------------VMRIINEPTAAAIAYGLDK 758
I+GL+ V+RIINEPTAAAIAYGLDK
Sbjct: 63 IAGLDVLSRTASERPPSRFRRRRPHGRSRVLRIINEPTAAAIAYGLDK 110
>SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1068
Score = 83.8 bits (198), Expect = 1e-16
Identities = 37/85 (43%), Positives = 59/85 (69%)
Frame = +3
Query: 510 VQTSQGDKVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTI 689
VQ +VF+PE++ AM+ T++K TAE L KVT V++VP+Y+ D QR+ DA
Sbjct: 162 VQYLGKQEVFSPEQVMAMLFTRLKTTAEIALKTKVTDCVISVPSYYTDRQRRCMLDASAT 221
Query: 690 SGLNVMRIINEPTAAAIAYGLDKKE 764
+GLN +R++N+ TA ++AYG+ K++
Sbjct: 222 AGLNCLRLMNDTTAVSLAYGIYKQD 246
Score = 40.7 bits (91), Expect = 0.001
Identities = 17/38 (44%), Positives = 26/38 (68%)
Frame = +1
Query: 373 LTTNPENTVFDAKRLIGREWSDQTVQHDVKFFPFKVVE 486
+ +N +NT+ KR IGR++SD VQ ++ P+KVVE
Sbjct: 115 MISNLKNTISQFKRFIGRKFSDPAVQKEIPHLPYKVVE 152
Score = 37.1 bits (82), Expect = 0.017
Identities = 17/38 (44%), Positives = 26/38 (68%)
Frame = +1
Query: 259 KNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQ 372
+ G +E +AN+ +R TPS+V+ D +RLIG + KNQ
Sbjct: 19 RGGGIETVANEFSDRCTPSFVSL-GDKQRLIGTSGKNQ 55
>SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36)
Length = 437
Score = 83.8 bits (198), Expect = 1e-16
Identities = 41/77 (53%), Positives = 54/77 (70%)
Frame = +3
Query: 534 VFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTISGLNVMRI 713
V PEEI V+ K++ AE L VT AV++VPA F+ QR AT A +++G+ V+R+
Sbjct: 143 VITPEEIGGYVIKKLRTMAERNLTTTVTKAVMSVPAEFDIKQRNATVKAASLAGITVLRV 202
Query: 714 INEPTAAAIAYGLDKKE 764
INEPTAAA+AYGL KKE
Sbjct: 203 INEPTAAALAYGLHKKE 219
Score = 64.1 bits (149), Expect = 1e-10
Identities = 28/73 (38%), Positives = 49/73 (67%)
Frame = +1
Query: 262 NGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPENTVFDAKRLIGREWSDQ 441
+GR++I +++ +++ PS VAFT G L+G+ A +Q NP+NT++DAKR IG+ ++ Q
Sbjct: 52 SGRIDIFSDENDDKVVPSLVAFTDKGV-LVGEKALSQAEINPKNTIYDAKRFIGKTFTKQ 110
Query: 442 TVQHDVKFFPFKV 480
+ K + F+V
Sbjct: 111 ELLEIGKLYQFRV 123
Score = 35.1 bits (77), Expect = 0.067
Identities = 14/15 (93%), Positives = 15/15 (100%)
Frame = +2
Query: 212 VIGIDLGTTYSCVGA 256
+IGIDLGTTYSCVGA
Sbjct: 33 IIGIDLGTTYSCVGA 47
>SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1019
Score = 66.9 bits (156), Expect = 2e-11
Identities = 31/77 (40%), Positives = 45/77 (58%)
Frame = +3
Query: 522 QGDKVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTISGLN 701
Q D F PEE+ M+L + E + + V+TVP +FN A+R+A A + GLN
Sbjct: 75 QSDVTFTPEELMGMILNHSRFIGEQFADHPIKDVVLTVPPFFNQAERRALLRAAELVGLN 134
Query: 702 VMRIINEPTAAAIAYGL 752
V++I+N TA A+ YGL
Sbjct: 135 VLQIMNSNTAVALNYGL 151
>SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07)
Length = 592
Score = 35.9 bits (79), Expect = 0.038
Identities = 17/30 (56%), Positives = 23/30 (76%)
Frame = +1
Query: 262 NGRVEIIANDQGNRITPSYVAFTQDGERLI 351
+G IIAND G+R+TP+ VAF+ D E+LI
Sbjct: 82 DGNTTIIANDAGDRVTPAMVAFS-DTEKLI 110
>SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16)
Length = 212
Score = 35.9 bits (79), Expect = 0.038
Identities = 15/25 (60%), Positives = 20/25 (80%)
Frame = +1
Query: 274 EIIANDQGNRITPSYVAFTQDGERL 348
++I N +G+R TPS VAFT +GERL
Sbjct: 7 KVIENAEGSRTTPSVVAFTPEGERL 31
>SB_8282| Best HMM Match : NHL (HMM E-Value=9.2e-23)
Length = 877
Score = 31.5 bits (68), Expect = 0.82
Identities = 17/51 (33%), Positives = 28/51 (54%)
Frame = +1
Query: 256 YKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPENTVFDA 408
Y N +V + A D G+++T Y A T +G+ L+ A + +T N + V A
Sbjct: 731 YHNRKVHVFARDDGSQVT-CYTAVTDEGDGLLN--APSGITVNSKGEVIVA 778
>SB_16422| Best HMM Match : HMG_box (HMM E-Value=8.7e-26)
Length = 245
Score = 30.3 bits (65), Expect = 1.9
Identities = 17/55 (30%), Positives = 23/55 (41%), Gaps = 4/55 (7%)
Frame = +1
Query: 382 NPENTVFDAKRLIGREWSDQTVQHDVKFF----PFKVVEKNSKPHVQYKLHKATK 534
NP+ FD R +G EW T + +F K K PH +Y+ K K
Sbjct: 41 NPQMHNFDISRKLGLEWQKLTEEEKAYYFEEAKKLKEEHKERYPHYKYQPRKRDK 95
>SB_41346| Best HMM Match : Ion_trans (HMM E-Value=0)
Length = 1264
Score = 29.5 bits (63), Expect = 3.3
Identities = 13/34 (38%), Positives = 20/34 (58%)
Frame = -1
Query: 449 CTVWSLHSRPIRRLASKTVFSGLVVSWFLAASPI 348
C VW + +RRL VF G+++ + +AAS I
Sbjct: 387 CAVWKVMRYKVRRLVEHKVFEGIIL-FLIAASSI 419
>SB_23114| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 286
Score = 28.7 bits (61), Expect = 5.8
Identities = 13/28 (46%), Positives = 18/28 (64%)
Frame = -1
Query: 86 INKNLNKIIFTATIRSL*NAFIKHLRSC 3
+N LN II+T R+ AF+K +RSC
Sbjct: 199 LNSCLNPIIYTCLNRTYRTAFLKLMRSC 226
>SB_42147| Best HMM Match : Thioredoxin (HMM E-Value=0.3)
Length = 787
Score = 28.7 bits (61), Expect = 5.8
Identities = 12/22 (54%), Positives = 15/22 (68%)
Frame = -1
Query: 431 HSRPIRRLASKTVFSGLVVSWF 366
H I RL+S+ VFSG V+ WF
Sbjct: 114 HKILIDRLSSRFVFSGTVIQWF 135
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 23,423,791
Number of Sequences: 59808
Number of extensions: 459530
Number of successful extensions: 1131
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 1014
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1124
length of database: 16,821,457
effective HSP length: 81
effective length of database: 11,977,009
effective search space used: 2215746665
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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