Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00443
(723 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 45 6e-05
At4g27670.1 68417.m03979 25.3 kDa small heat shock protein, chlo... 44 1e-04
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 44 1e-04
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 43 2e-04
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 42 3e-04
At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein (HS... 41 0.001
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 40 0.002
At1g52560.1 68414.m05933 26.5 kDa class I small heat shock prote... 36 0.021
At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock pro... 32 0.33
At5g52920.1 68418.m06567 pyruvate kinase, putative similar to py... 29 4.1
At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family prote... 29 4.1
At4g34020.1 68417.m04827 DJ-1 family protein similar to CAP1 [Ra... 29 4.1
At5g55300.1 68418.m06891 DNA topoisomerase I identical to Swiss-... 28 5.5
At3g12020.1 68416.m01490 kinesin motor protein-related similar t... 28 5.5
At1g53330.1 68414.m06045 pentatricopeptide (PPR) repeat-containi... 28 5.5
At3g27490.1 68416.m03437 DC1 domain-containing protein contains ... 27 9.5
At1g27680.1 68414.m03383 glucose-1-phosphate adenylyltransferase... 27 9.5
>At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein
(HSP17.8-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]; contains Pfam
PF00011: Hsp20/alpha crystallin family
Length = 157
Score = 44.8 bits (101), Expect = 6e-05
Identities = 29/88 (32%), Positives = 47/88 (53%), Gaps = 8/88 (9%)
Frame = +1
Query: 277 DVQHFSPEEISVKTADGYVI-VEGKH----EERQDEHGYISR---QFTRRYALPENCNPD 432
D+ EE+ V+ D V+ + G+ EE+QD + R QF+R++ LPEN D
Sbjct: 64 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMD 123
Query: 433 TVESRLSSDGVLTVIAPRTPAATKNGEL 516
V++ + +GVLTV P+ A K ++
Sbjct: 124 QVKASM-ENGVLTVTVPKVEEAKKKAQV 150
>At4g27670.1 68417.m03979 25.3 kDa small heat shock protein,
chloroplast precursor (HSP25.3-P) identical to small
heat shock protein, chloroplast precursor SP:P31170 from
[Arabidopsis thaliana]; identified in Scharf, K-D., et
al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 227
Score = 43.6 bits (98), Expect = 1e-04
Identities = 19/85 (22%), Positives = 43/85 (50%), Gaps = 3/85 (3%)
Frame = +1
Query: 259 KFQVNLDVQHFSPEEISVKTADGYVIVEGKHEERQDEHGYISRQ---FTRRYALPENCNP 429
+ ++ D+ S E++ + D ++++G+ ++ + + R + R LP+NC
Sbjct: 137 EIKMRFDMPGLSKEDVKISVEDNVLVIKGEQKKEDSDDSWSGRSVSSYGTRLQLPDNCEK 196
Query: 430 DTVESRLSSDGVLTVIAPRTPAATK 504
D +++ L +GVL + P+T K
Sbjct: 197 DKIKAEL-KNGVLFITIPKTKVERK 220
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 43.6 bits (98), Expect = 1e-04
Identities = 25/80 (31%), Positives = 46/80 (57%), Gaps = 8/80 (10%)
Frame = +1
Query: 277 DVQHFSPEEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFTRRYALPENCNPD 432
D+ EE+ V+ DG ++ + G+ +EE+ D+ + R +FTRR+ LPEN +
Sbjct: 66 DLPGLRKEEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTRRFRLPENAKME 125
Query: 433 TVESRLSSDGVLTVIAPRTP 492
+++ + +GVL+V P+ P
Sbjct: 126 EIKASM-ENGVLSVTVPKVP 144
>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
(HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
crystallin family; identified in Scharf, K-D., et al,
Cell Stress & Chaperones (2001) 6: 225-237.
Length = 153
Score = 42.7 bits (96), Expect = 2e-04
Identities = 30/88 (34%), Positives = 49/88 (55%), Gaps = 8/88 (9%)
Frame = +1
Query: 277 DVQHFSPEEISVKTADGYVI-VEG-KHEERQDEHGYISR------QFTRRYALPENCNPD 432
D+ EE+ V+ + V+ + G +H E++D++ R QFTRR+ LPEN D
Sbjct: 62 DLPGLKKEEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMD 121
Query: 433 TVESRLSSDGVLTVIAPRTPAATKNGEL 516
V++ + +GVLTV P+ A TK ++
Sbjct: 122 QVKAAM-ENGVLTVTVPK--AETKKADV 146
>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
(HSP18.1-CI) identical to 18.2 kDa class I heat shock
protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
contains Pfam profile: PF00011 Hsp20/alpha crystallin
family
Length = 161
Score = 42.3 bits (95), Expect = 3e-04
Identities = 26/80 (32%), Positives = 45/80 (56%), Gaps = 8/80 (10%)
Frame = +1
Query: 277 DVQHFSPEEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFTRRYALPENCNPD 432
D+ EE+ V+ D V+ + G+ +EE+ D+ + R +F RR+ LPEN +
Sbjct: 68 DLPGLKKEEVKVEVEDKNVLQISGERSKENEEKNDKWHRVERASGKFMRRFRLPENAKME 127
Query: 433 TVESRLSSDGVLTVIAPRTP 492
V++ + +GVLTV+ P+ P
Sbjct: 128 EVKATM-ENGVLTVVVPKAP 146
>At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein
(HSP17.6A-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]
Length = 155
Score = 40.7 bits (91), Expect = 0.001
Identities = 27/88 (30%), Positives = 45/88 (51%), Gaps = 8/88 (9%)
Frame = +1
Query: 277 DVQHFSPEEISVKTADGYVI-VEGKH----EERQDEHGYISRQ---FTRRYALPENCNPD 432
D+ EE+ V+ D V+ + G+ EE+QD + R F+R++ LPEN D
Sbjct: 62 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGGFSRKFRLPENVKMD 121
Query: 433 TVESRLSSDGVLTVIAPRTPAATKNGEL 516
V++ + +GVLTV P+ K ++
Sbjct: 122 QVKASM-ENGVLTVTVPKVETNKKKAQV 148
>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
(HSP17.4-CI) identical to 17.4 kDa class I heat shock
protein SP:P19036 from [Arabidopsis thaliana]
Length = 156
Score = 39.5 bits (88), Expect = 0.002
Identities = 25/78 (32%), Positives = 43/78 (55%), Gaps = 8/78 (10%)
Frame = +1
Query: 277 DVQHFSPEEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFTRRYALPENCNPD 432
DV EE+ V+ DG ++ + G+ +EE+ D + R +F RR+ LPEN +
Sbjct: 65 DVPGLKKEEVKVEVEDGNILQISGERSSENEEKSDTWHRVERSSGKFMRRFRLPENAKVE 124
Query: 433 TVESRLSSDGVLTVIAPR 486
V++ + +GVL+V P+
Sbjct: 125 EVKASM-ENGVLSVTVPK 141
>At1g52560.1 68414.m05933 26.5 kDa class I small heat shock
protein-like (HSP26.5-P) contains Pfam profile: PF00011
Hsp20/alpha crystallin family; identified in Scharf,
K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 232
Score = 36.3 bits (80), Expect = 0.021
Identities = 21/93 (22%), Positives = 47/93 (50%), Gaps = 9/93 (9%)
Frame = +1
Query: 256 DKFQVNLDVQHFSPEEISVKTADGYVIVEGKHEERQD-----EHGYISRQ----FTRRYA 408
D +++ +V + E++ + DG + ++G H+ ++ E Y S + + +
Sbjct: 134 DCYKLRYEVPGLTKEDVKITVNDGILTIKGDHKAEEEKGSPEEDEYWSSKSYGYYNTSLS 193
Query: 409 LPENCNPDTVESRLSSDGVLTVIAPRTPAATKN 507
LP++ + +++ L + GVL ++ PRT KN
Sbjct: 194 LPDDAKVEDIKAELKN-GVLNLVIPRTEKPKKN 225
>At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock
protein (HSP26.5-P) contains Pfam profile: PF00011
Hsp20/alpha crystallin family: identified in Scharf,
K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 134
Score = 32.3 bits (70), Expect = 0.33
Identities = 20/77 (25%), Positives = 34/77 (44%)
Frame = +1
Query: 256 DKFQVNLDVQHFSPEEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDT 435
D ++D+ EEI V+ D ++ + F R++ LPE+ +
Sbjct: 35 DSHTFSVDLPGLRKEEIKVEIEDSIYLIIRTEATPMSPPDQPLKTFKRKFRLPESIDMIG 94
Query: 436 VESRLSSDGVLTVIAPR 486
+ + DGVLTVI P+
Sbjct: 95 ISAGYE-DGVLTVIVPK 110
>At5g52920.1 68418.m06567 pyruvate kinase, putative similar to
pyruvate kinase isozyme G, chloroplast precursor
[Nicotiana tabacum] SWISS-PROT:Q40546
Length = 579
Score = 28.7 bits (61), Expect = 4.1
Identities = 18/54 (33%), Positives = 29/54 (53%)
Frame = +1
Query: 448 LSSDGVLTVIAPRTPAATKNGELFPSLKPVRSGRRLRSPLRKLRATKQNNDLGT 609
L + GV+ +A RT A +GE+ P+L ++ + S + AT +N LGT
Sbjct: 427 LKAAGVMHTVALRTEATITSGEMPPNLG--QAFKNHMSEMFAYHATMMSNTLGT 478
>At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 822
Score = 28.7 bits (61), Expect = 4.1
Identities = 16/54 (29%), Positives = 22/54 (40%)
Frame = +1
Query: 298 EEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRLSSD 459
+EI D Y EG EE ++ R + L E+C + VES D
Sbjct: 48 DEIDSAYGDFYEYEEGVPEEESRKNNRYERVVNYEFELAEDCEDENVESEDDDD 101
>At4g34020.1 68417.m04827 DJ-1 family protein similar to CAP1
[Rattus norvegicus] GI:3250916, ThiJ protein,
Escherichia coli, PIR:H64771; contains Pfam profile
PF01965 ThiJ/PfpI family
Length = 472
Score = 28.7 bits (61), Expect = 4.1
Identities = 16/43 (37%), Positives = 20/43 (46%)
Frame = -1
Query: 447 PRFDSVRVAVFRQSVTPRELTRDVAMLVLPLLVLTFDDDVAVG 319
P F SV V T R+ T + + P +V T D DV VG
Sbjct: 36 PSFSSVSVVSSSLGTTRRDRTLKLRSSMSPGMVTTLDSDVGVG 78
>At5g55300.1 68418.m06891 DNA topoisomerase I identical to
Swiss-Prot:P30181 DNA topoisomerase I [Arabidopsis
thaliana]
Length = 916
Score = 28.3 bits (60), Expect = 5.5
Identities = 11/37 (29%), Positives = 18/37 (48%)
Frame = +3
Query: 480 SQDSGCHEERRAVPITQTGPVRKEIKEPTAEVESNET 590
+Q + +AVP T+ P+R + P SN+T
Sbjct: 37 NQSKSNSQRSKAVPTTKVSPMRSPVTSPNGTTPSNKT 73
>At3g12020.1 68416.m01490 kinesin motor protein-related similar to
putative kinesin heavy chain GB:AAD23684 GI:4567271 from
[Arabidopsis thaliana]
Length = 1030
Score = 28.3 bits (60), Expect = 5.5
Identities = 21/64 (32%), Positives = 31/64 (48%), Gaps = 1/64 (1%)
Frame = +1
Query: 322 DGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVE-SRLSSDGVLTVIAPRTPAA 498
D YV VEG HE R ++ Y + TR++ L P + S +SD V + TP+
Sbjct: 524 DLYVSVEGNHEIR--DNAYREEKKTRKHGLLNWLKPKKRDHSSSASDQSSVVKSNSTPST 581
Query: 499 TKNG 510
+ G
Sbjct: 582 PQGG 585
>At1g53330.1 68414.m06045 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 471
Score = 28.3 bits (60), Expect = 5.5
Identities = 22/74 (29%), Positives = 33/74 (44%)
Frame = +1
Query: 355 ERQDEHGYISRQFTRRYALPENCNPDTVESRLSSDGVLTVIAPRTPAATKNGELFPSLKP 534
++ +E Y+ RR C+PDT+ R+ DG+ + A + LF KP
Sbjct: 342 KKWEEATYLFEDMPRR-----GCSPDTLSYRIVFDGLCEGLQFEEAAVILDEMLFKGYKP 396
Query: 535 VRSGRRLRSPLRKL 576
R RL L+KL
Sbjct: 397 RRD--RLEGFLQKL 408
>At3g27490.1 68416.m03437 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 682
Score = 27.5 bits (58), Expect = 9.5
Identities = 13/47 (27%), Positives = 23/47 (48%)
Frame = +2
Query: 521 HHSNRSGPEGD*GAHCGS*EQRNKTMISERCESKKCSVC*FYLHSSC 661
H+ G +G+ G++CG+ + + +C CS F LH +C
Sbjct: 383 HNLMSLGKDGEEGSYCGACARPISSYTFYKCSRSDCS---FILHETC 426
>At1g27680.1 68414.m03383 glucose-1-phosphate adenylyltransferase
large subunit 2 (APL2) / ADP-glucose pyrophosphorylase
identical to SP|P55230
Length = 518
Score = 27.5 bits (58), Expect = 9.5
Identities = 15/59 (25%), Positives = 26/59 (44%)
Frame = +1
Query: 298 EEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRLSSDGVLTV 474
E+ K + +I+ G H R D ++ + + +C P ESR S G+L +
Sbjct: 198 EDAKTKNVEHVLILSGDHLYRMDYMNFVQKHIESNADITVSCLP-MDESRASDFGLLKI 255
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,779,951
Number of Sequences: 28952
Number of extensions: 300991
Number of successful extensions: 990
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 957
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 989
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1575119672
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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