SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00429
         (797 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC14C8.07c |cdc18||MCM loader|Schizosaccharomyces pombe|chr 2|...    36   0.007
SPBC646.14c |orc5||origin recognition complex subunit Orc5|Schiz...    32   0.11 
SPBC29A10.15 |orc1|orp1, cdc30|origin recognition complex subuni...    31   0.14 
SPAC57A7.10c |sec21||coatomer gamma subunit Sec21 |Schizosacchar...    29   1.0  
SPBC16C6.09 |ogm4|oma4|protein O-mannosyltransferase Ogm4|Schizo...    28   1.3  
SPBC119.15 |||AAA family ATPase, unknown biological role|Schizos...    28   1.8  
SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit ...    27   2.4  
SPBC887.14c |pfh1|pif1|pif1 helicase homolog Pfh1|Schizosaccharo...    27   2.4  
SPBC23G7.12c |rpt6|let1|19S proteasome regulatory subunit Rpt6|S...    26   5.4  
SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein 5|...    26   5.4  
SPBC16C6.07c |rpt1||19S proteasome regulatory subunit Rpt1|Schiz...    26   7.2  
SPAP27G11.06c |||AP-1 adaptor complex subunit |Schizosaccharomyc...    26   7.2  
SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase Tor2|S...    26   7.2  
SPBC11B10.09 |cdc2|swo2|cyclin-dependent protein kinase Cdc2|Sch...    26   7.2  
SPCC1393.05 |||sequence orphan|Schizosaccharomyces pombe|chr 3||...    26   7.2  
SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa...    25   9.5  
SPBC20F10.02c |||DUF1741 family protein|Schizosaccharomyces pomb...    25   9.5  
SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual      25   9.5  
SPBC3B9.02c |cwf28||splicing factor Cwf28|Schizosaccharomyces po...    25   9.5  

>SPBC14C8.07c |cdc18||MCM loader|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 577

 Score = 35.9 bits (79), Expect = 0.007
 Identities = 29/97 (29%), Positives = 44/97 (45%), Gaps = 8/97 (8%)
 Frame = +1

Query: 205 NVMLTNCSQLFKL*TCIVDCIECYSSKIMFENILSALND----ENED----VKCENLMDF 360
           NV+    S   K+  C ++C+     K +FE I S +      ENED     +CE    F
Sbjct: 211 NVLDHVVSDYPKVNVCYINCMTINEPKAIFEKIHSKIVKEEILENEDHHINFQCELESHF 270

Query: 361 INCLNRLQNNYEPIVIVFDRVERLRNMDQNLLPVLLK 471
               N L   Y P++IV D ++ L   +Q +L  L +
Sbjct: 271 TQSANEL---YNPVIIVLDEMDHLIAREQQVLYTLFE 304



 Score = 27.1 bits (57), Expect = 3.1
 Identities = 15/48 (31%), Positives = 29/48 (60%), Gaps = 1/48 (2%)
 Frame = +2

Query: 98  VVCRENQLNDLFNLFGDDNEP-LPCSIFISGSMATGKTLCLQTVLNYL 238
           VV REN+ + + + F    +     ++++SG+  TGKT+ L  VL+++
Sbjct: 169 VVGRENEKSIVESFFRQHLDANAGGALYVSGAPGTGKTVLLHNVLDHV 216


>SPBC646.14c |orc5||origin recognition complex subunit
           Orc5|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 455

 Score = 31.9 bits (69), Expect = 0.11
 Identities = 19/58 (32%), Positives = 31/58 (53%)
 Frame = +2

Query: 80  DEIYSKVVCRENQLNDLFNLFGDDNEPLPCSIFISGSMATGKTLCLQTVLNYLSYKHV 253
           DE+   V CRE+Q+  L  L  + +  +P SI + G  +T KT  L+T  +    ++V
Sbjct: 8   DELKKNVFCREDQIKKLSCLLFNKDCRVP-SIVLYGVASTAKTFLLRTAFDLSKEENV 64


>SPBC29A10.15 |orc1|orp1, cdc30|origin recognition complex subunit
           Orc1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 707

 Score = 31.5 bits (68), Expect = 0.14
 Identities = 16/47 (34%), Positives = 26/47 (55%), Gaps = 1/47 (2%)
 Frame = +2

Query: 92  SKVVCRENQLNDLF-NLFGDDNEPLPCSIFISGSMATGKTLCLQTVL 229
           S + CR+N+ + +F NL     E     ++ISG+  TGKT  +  V+
Sbjct: 336 STLQCRDNEFSTIFSNLESAIEEETGACLYISGTPGTGKTATVHEVI 382


>SPAC57A7.10c |sec21||coatomer gamma subunit Sec21
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 905

 Score = 28.7 bits (61), Expect = 1.0
 Identities = 11/27 (40%), Positives = 18/27 (66%)
 Frame = -1

Query: 551 TEFEIKIFEY*MRHHIVQMFNLQNSLS 471
           TEF +K+ ++  + H+V  F L N+LS
Sbjct: 676 TEFVVKVVKHVFKDHLVVQFQLHNTLS 702


>SPBC16C6.09 |ogm4|oma4|protein O-mannosyltransferase
           Ogm4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 778

 Score = 28.3 bits (60), Expect = 1.3
 Identities = 11/21 (52%), Positives = 13/21 (61%)
 Frame = -3

Query: 345 FTFHVLVFIVEGRQNVLEHYL 283
           + FH L F + GRQ  L HYL
Sbjct: 655 YVFHYLPFFIMGRQLFLHHYL 675


>SPBC119.15 |||AAA family ATPase, unknown biological
           role|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 367

 Score = 27.9 bits (59), Expect = 1.8
 Identities = 12/34 (35%), Positives = 19/34 (55%)
 Frame = +2

Query: 149 DNEPLPCSIFISGSMATGKTLCLQTVLNYLSYKH 250
           D E  PC+I + G   +GKT  +Q +  +L  K+
Sbjct: 3   DKEKKPCAIIVVGMAGSGKTTFMQQLNAHLHSKN 36


>SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit
           Cct4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 527

 Score = 27.5 bits (58), Expect = 2.4
 Identities = 15/39 (38%), Positives = 22/39 (56%)
 Frame = +3

Query: 627 KSFVQHLLNHFDIEDETTELIEKPELFANFLNAFLSVSI 743
           +SF +  L H D+ +ET+   EK   F+   NA  +VSI
Sbjct: 338 ESFAEDKLGHADLVEETSSSGEKIVKFSGVKNAGKTVSI 376


>SPBC887.14c |pfh1|pif1|pif1 helicase homolog
           Pfh1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 805

 Score = 27.5 bits (58), Expect = 2.4
 Identities = 11/27 (40%), Positives = 18/27 (66%)
 Frame = +2

Query: 170 SIFISGSMATGKTLCLQTVLNYLSYKH 250
           SIF +GS  TGK++ L+ ++  L  K+
Sbjct: 327 SIFFTGSAGTGKSVLLRKIIEVLKSKY 353


>SPBC23G7.12c |rpt6|let1|19S proteasome regulatory subunit
           Rpt6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 403

 Score = 26.2 bits (55), Expect = 5.4
 Identities = 14/49 (28%), Positives = 24/49 (48%)
 Frame = +2

Query: 137 LFGDDNEPLPCSIFISGSMATGKTLCLQTVLNYLSYKHVLSTVSSVIRQ 283
           LF     P P  I + G   TGKTL  + V ++   K +  + S ++++
Sbjct: 170 LFESLGIPQPKGILLYGPPGTGKTLLARAVAHHTDCKFIRVSGSELVQK 218


>SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein
           5|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 736

 Score = 26.2 bits (55), Expect = 5.4
 Identities = 13/38 (34%), Positives = 19/38 (50%)
 Frame = +1

Query: 247 TCIVDCIECYSSKIMFENILSALNDENEDVKCENLMDF 360
           T + +    Y S++  ENIL +   ENE    E + DF
Sbjct: 300 TTLSEISPIYDSELAIENILESKCFENEQAIIEGVKDF 337


>SPBC16C6.07c |rpt1||19S proteasome regulatory subunit
           Rpt1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 438

 Score = 25.8 bits (54), Expect = 7.2
 Identities = 11/23 (47%), Positives = 13/23 (56%)
 Frame = +2

Query: 164 PCSIFISGSMATGKTLCLQTVLN 232
           P  I + G   TGKTLC + V N
Sbjct: 213 PKGIMLYGPPGTGKTLCARAVAN 235


>SPAP27G11.06c |||AP-1 adaptor complex subunit |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 162

 Score = 25.8 bits (54), Expect = 7.2
 Identities = 16/60 (26%), Positives = 32/60 (53%)
 Frame = +1

Query: 319 DENEDVKCENLMDFINCLNRLQNNYEPIVIVFDRVERLRNMDQNLLPVLLKLREFCKLNI 498
           D+NE +  E +  F+ CL++   N   + ++F+  +    M++ LL    +L+E  K N+
Sbjct: 75  DDNELIILEVIHKFVECLDKYFGNVCELDLIFNFEKAYYVMEELLLAG--ELQESSKTNV 132


>SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase
            Tor2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2337

 Score = 25.8 bits (54), Expect = 7.2
 Identities = 10/38 (26%), Positives = 22/38 (57%)
 Frame = +3

Query: 501  HDMVTHLIFENFNFKFGVREPIKLYFPNYNKEELFRII 614
            H+++   I  +  +  G+ E  +LYF ++N E +F ++
Sbjct: 1816 HELIRAAILWHEQWHEGLEEASRLYFGDHNIEGMFAVL 1853


>SPBC11B10.09 |cdc2|swo2|cyclin-dependent protein kinase
           Cdc2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 297

 Score = 25.8 bits (54), Expect = 7.2
 Identities = 12/40 (30%), Positives = 22/40 (55%)
 Frame = +1

Query: 277 SSKIMFENILSALNDENEDVKCENLMDFINCLNRLQNNYE 396
           S+ I   ++L  +NDEN    C  L+D ++  ++L   +E
Sbjct: 46  STAIREISLLKEVNDENNRSNCVRLLDILHAESKLYLVFE 85


>SPCC1393.05 |||sequence orphan|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 956

 Score = 25.8 bits (54), Expect = 7.2
 Identities = 11/36 (30%), Positives = 19/36 (52%)
 Frame = +2

Query: 86  IYSKVVCRENQLNDLFNLFGDDNEPLPCSIFISGSM 193
           +++K   +    ND F +F DD + + C IF   S+
Sbjct: 308 VFAKNPSKTPPCNDNFKIFSDDIKLIGCKIFAGVSL 343


>SPAC19A8.08 |upf2||nonsense-mediated decay protein
           Upf2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1049

 Score = 25.4 bits (53), Expect = 9.5
 Identities = 20/77 (25%), Positives = 41/77 (53%), Gaps = 4/77 (5%)
 Frame = +1

Query: 265 IECYSSKIMFENILSALNDENEDVKCENLMDFINCLNR--LQNNYEPIV--IVFDRVERL 432
           ++ +   I  + + SAL  +++ V  EN + F+N   R  + +  + +    ++D ++ +
Sbjct: 600 MQSFLEAIQKKKLASALASQDQLV-LENALHFVNPPKRGIIVSKKKSLKEEFLYDLIQ-I 657

Query: 433 RNMDQNLLPVLLKLREF 483
           R  D N+ P LL LR+F
Sbjct: 658 RLKDDNVFPTLLLLRKF 674


>SPBC20F10.02c |||DUF1741 family protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 600

 Score = 25.4 bits (53), Expect = 9.5
 Identities = 7/19 (36%), Positives = 13/19 (68%)
 Frame = +3

Query: 609 IIFLYQKSFVQHLLNHFDI 665
           + FLY  SF+ H+  ++D+
Sbjct: 157 VSFLYNSSFISHIYGNYDV 175


>SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1489

 Score = 25.4 bits (53), Expect = 9.5
 Identities = 18/66 (27%), Positives = 39/66 (59%), Gaps = 5/66 (7%)
 Frame = +3

Query: 552  VREPIKLYFPNYNKEELFRII--FLYQKSFVQHLL---NHFDIEDETTELIEKPELFANF 716
            ++  ++ YFP  +KEE+F +I  F+Y  ++V  +L   ++F + D +   +++  L+   
Sbjct: 1011 LKNSLQRYFPAAHKEEIFGVIGKFVYW-AYVAPVLVSPDNFKLVDGSITALQRKNLYT-- 1067

Query: 717  LNAFLS 734
            L++ LS
Sbjct: 1068 LSSILS 1073


>SPBC3B9.02c |cwf28||splicing factor Cwf28|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 381

 Score = 25.4 bits (53), Expect = 9.5
 Identities = 18/63 (28%), Positives = 28/63 (44%), Gaps = 1/63 (1%)
 Frame = +1

Query: 277 SSKIMFENILSALNDENEDVKCENLMDFINCLNRLQNNYEPIVIVFDRVERLRNM-DQNL 453
           S+ I+ E +   LND N+DVK  N  +       + N  +      D +    NM D + 
Sbjct: 131 STDILPEEVQLVLNDLNDDVKSANSANLQPITTNVVNEKDAYRKDIDELPEPSNMKDYSE 190

Query: 454 LPV 462
           +PV
Sbjct: 191 IPV 193


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,044,260
Number of Sequences: 5004
Number of extensions: 61958
Number of successful extensions: 208
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 199
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 208
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 389395636
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -