SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00414
         (710 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAP7G5.05 |rpl1002|rpl10-2, rpl10|60S ribosomal protein L10|Sch...   142   6e-35
SPBC18E5.04 |rpl1001|rpl10-1, rpl10|60S ribosomal protein L10|Sc...   142   6e-35
SPBC1348.02 |||S. pombe specific 5Tm protein family|Schizosaccha...    29   0.50 
SPBPB2B2.19c |||S. pombe specific 5Tm protein family|Schizosacch...    29   0.50 
SPAC977.01 |||S. pombe specific 5Tm protein family|Schizosacchar...    29   0.50 
SPAC750.05c |||S. pombe specific 5Tm protein family|Schizosaccha...    29   0.50 
SPAC19D5.03 |cid1||poly|Schizosaccharomyces pombe|chr 1|||Manual       29   0.66 
SPCC1827.04 |||ankyrin repeat protein, unknown biological role|S...    27   2.0  
SPCC622.15c |||sequence orphan|Schizosaccharomyces pombe|chr 3||...    27   2.6  
SPBC1861.02 |abp2||ARS binding protein Abp2|Schizosaccharomyces ...    27   2.6  
SPBC354.10 |||RNAPII degradation factor |Schizosaccharomyces pom...    27   3.5  
SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2 |Schiz...    26   4.6  
SPAC688.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    26   6.1  
SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pomb...    25   8.1  
SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|...    25   8.1  
SPBC577.09 |||ERCC-8 homolog |Schizosaccharomyces pombe|chr 2|||...    25   8.1  
SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces...    25   8.1  
SPCC24B10.07 |gad8||serine/threonine protein kinase Gad8 |Schizo...    25   8.1  
SPAC3A12.05c |taf2||TATA-binding protein associated factor Taf2|...    25   8.1  
SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce...    25   8.1  

>SPAP7G5.05 |rpl1002|rpl10-2, rpl10|60S ribosomal protein
           L10|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 221

 Score =  142 bits (343), Expect = 6e-35
 Identities = 61/75 (81%), Positives = 68/75 (90%)
 Frame = +3

Query: 30  MGRRPARCYRYCKNKPYPKSRFCRGVPDPKIRIFDLGKKRATVDDFPLCVHLVSDEYEQL 209
           M RRPARCYRYCKNKPYPKSR+ R VPD KIRIFDLG+KRA VD+FPLC+HLVS+EYEQ+
Sbjct: 1   MARRPARCYRYCKNKPYPKSRYNRAVPDSKIRIFDLGRKRAGVDEFPLCIHLVSNEYEQI 60

Query: 210 SSEALEAGRICCNKY 254
           +SEALEA RIC NKY
Sbjct: 61  TSEALEAARICANKY 75



 Score =  134 bits (325), Expect = 9e-33
 Identities = 66/100 (66%), Positives = 76/100 (76%), Gaps = 1/100 (1%)
 Frame = +2

Query: 254 LVKNCGKDQFHIRMRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVRIGQPI 433
           LVK  GKD FH+R+R HPFHV+RINKMLSCAGADRLQTGMR AFGKP G VARV IGQ +
Sbjct: 76  LVKIGGKDSFHLRVRAHPFHVVRINKMLSCAGADRLQTGMRHAFGKPNGLVARVNIGQVL 135

Query: 434 MSVRSSDRWKAQVIEALRRAKFKFPDVKRSTYQRS-GVSQ 550
           MSVR+ D  +A  IEALRR ++KFP  +R    +  G SQ
Sbjct: 136 MSVRTKDSSRATAIEALRRCQYKFPGQQRIIVSKKWGFSQ 175



 Score = 57.6 bits (133), Expect = 2e-09
 Identities = 23/52 (44%), Positives = 34/52 (65%)
 Frame = +1

Query: 490 CQVQVPRRQKIYVSKKWGFTKYERDEFEKLREEGRLANDGCIVQYRPEHGPL 645
           CQ + P +Q+I VSKKWGF++Y RDE+ + R  G +  DGC  ++  + G L
Sbjct: 155 CQYKFPGQQRIIVSKKWGFSQYARDEYIEKRSRGEIIPDGCYAKFLNKRGSL 206


>SPBC18E5.04 |rpl1001|rpl10-1, rpl10|60S ribosomal protein
           L10|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 221

 Score =  142 bits (343), Expect = 6e-35
 Identities = 61/75 (81%), Positives = 68/75 (90%)
 Frame = +3

Query: 30  MGRRPARCYRYCKNKPYPKSRFCRGVPDPKIRIFDLGKKRATVDDFPLCVHLVSDEYEQL 209
           M RRPARCYRYCKNKPYPKSR+ R VPD KIRIFDLG+KRA VD+FPLC+HLVS+EYEQ+
Sbjct: 1   MARRPARCYRYCKNKPYPKSRYNRAVPDSKIRIFDLGRKRAGVDEFPLCIHLVSNEYEQI 60

Query: 210 SSEALEAGRICCNKY 254
           +SEALEA RIC NKY
Sbjct: 61  TSEALEAARICANKY 75



 Score =  134 bits (324), Expect = 1e-32
 Identities = 66/100 (66%), Positives = 76/100 (76%), Gaps = 1/100 (1%)
 Frame = +2

Query: 254 LVKNCGKDQFHIRMRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVRIGQPI 433
           LVK  GKD FH+R+R HPFHV+RINKMLSCAGADRLQTGMR AFGKP G VARV IGQ +
Sbjct: 76  LVKIGGKDSFHLRVRAHPFHVVRINKMLSCAGADRLQTGMRHAFGKPNGLVARVNIGQIL 135

Query: 434 MSVRSSDRWKAQVIEALRRAKFKFPDVKRSTYQRS-GVSQ 550
           MSVR+ D  +A  IEALRR ++KFP  +R    +  G SQ
Sbjct: 136 MSVRTKDSSRATAIEALRRCQYKFPGQQRIIVSKKWGFSQ 175



 Score = 57.6 bits (133), Expect = 2e-09
 Identities = 23/52 (44%), Positives = 34/52 (65%)
 Frame = +1

Query: 490 CQVQVPRRQKIYVSKKWGFTKYERDEFEKLREEGRLANDGCIVQYRPEHGPL 645
           CQ + P +Q+I VSKKWGF++Y RDE+ + R  G +  DGC  ++  + G L
Sbjct: 155 CQYKFPGQQRIIVSKKWGFSQYARDEYIEKRSRGEIIPDGCYAKFLNKRGSL 206


>SPBC1348.02 |||S. pombe specific 5Tm protein
           family|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 344

 Score = 29.5 bits (63), Expect = 0.50
 Identities = 12/35 (34%), Positives = 20/35 (57%)
 Frame = -3

Query: 600 SEAALFTQLLKLITFILCETPLL*YVDLLTSGNLN 496
           S+A  F ++L    FI C +P+L Y+  +  GN +
Sbjct: 263 SKARFFRRMLNAFIFIFCASPMLHYLSNIIFGNFD 297


>SPBPB2B2.19c |||S. pombe specific 5Tm protein
           family|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 344

 Score = 29.5 bits (63), Expect = 0.50
 Identities = 12/35 (34%), Positives = 20/35 (57%)
 Frame = -3

Query: 600 SEAALFTQLLKLITFILCETPLL*YVDLLTSGNLN 496
           S+A  F ++L    FI C +P+L Y+  +  GN +
Sbjct: 263 SKARFFRRMLNAFIFIFCASPMLHYLSNIIFGNFD 297


>SPAC977.01 |||S. pombe specific 5Tm protein
           family|Schizosaccharomyces pombe|chr 1||Partial|Manual
          Length = 316

 Score = 29.5 bits (63), Expect = 0.50
 Identities = 12/35 (34%), Positives = 20/35 (57%)
 Frame = -3

Query: 600 SEAALFTQLLKLITFILCETPLL*YVDLLTSGNLN 496
           S+A  F ++L    FI C +P+L Y+  +  GN +
Sbjct: 235 SKARFFRRMLNAFIFIFCASPMLHYLSNIIFGNFD 269


>SPAC750.05c |||S. pombe specific 5Tm protein
           family|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 344

 Score = 29.5 bits (63), Expect = 0.50
 Identities = 12/35 (34%), Positives = 20/35 (57%)
 Frame = -3

Query: 600 SEAALFTQLLKLITFILCETPLL*YVDLLTSGNLN 496
           S+A  F ++L    FI C +P+L Y+  +  GN +
Sbjct: 263 SKARFFRRMLNAFIFIFCASPMLHYLSNIIFGNFD 297


>SPAC19D5.03 |cid1||poly|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 405

 Score = 29.1 bits (62), Expect = 0.66
 Identities = 18/61 (29%), Positives = 32/61 (52%), Gaps = 1/61 (1%)
 Frame = +3

Query: 54  YRYCKNKPYPKSRFCRGVPDP-KIRIFDLGKKRATVDDFPLCVHLVSDEYEQLSSEALEA 230
           Y+   N     ++FC  V +  KI   +  +KRA +D   LC+  +S + E ++  +LE+
Sbjct: 34  YQKVPNSHKEFTKFCYEVYNEIKISDKEFKEKRAALDTLRLCLKRISPDAELVAFGSLES 93

Query: 231 G 233
           G
Sbjct: 94  G 94


>SPCC1827.04 |||ankyrin repeat protein, unknown biological
           role|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 600

 Score = 27.5 bits (58), Expect = 2.0
 Identities = 14/41 (34%), Positives = 23/41 (56%), Gaps = 1/41 (2%)
 Frame = +3

Query: 111 DPKIRIFDLGKKRATVDDFPLCV-HLVSDEYEQLSSEALEA 230
           DPK+R+F    KRAT  +   C   LV+ +   + S +++A
Sbjct: 320 DPKLRVFPFTTKRATHSELLRCYKELVTPKISHVDSISIKA 360


>SPCC622.15c |||sequence orphan|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 557

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 15/39 (38%), Positives = 22/39 (56%), Gaps = 1/39 (2%)
 Frame = -3

Query: 276 SFPQFFTSTYCSRYVLPPKPLS-SAVHIRRTPSARTVES 163
           SFPQ + ST  + Y   P P S S +++   P+A T+ S
Sbjct: 466 SFPQNYDSTVPAVYPYMPPPFSGSDMNVMSNPAADTLRS 504


>SPBC1861.02 |abp2||ARS binding protein Abp2|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 527

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 29/86 (33%), Positives = 40/86 (46%)
 Frame = +1

Query: 67  KINRIRNRGSVGVYLIPRSVSSIWVRRERPLTTFHCACTWCPTNMNS*AQRLWRQDVSAA 246
           KI R+R+  SV + L   S+S  W  R   L   H   T   +  +  A RL  + VS  
Sbjct: 340 KIKRLRSEPSVSLTL---SIS--WYERFEKL--MHAQNTMLRSAFSHVA-RLPMETVSQL 391

Query: 247 ISTRKELRKGSVPYPHETSPFPRYPH 324
           +S   E     +P P ETSP P+ P+
Sbjct: 392 LSHYTETISQYLP-PSETSPIPKTPN 416


>SPBC354.10 |||RNAPII degradation factor |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 963

 Score = 26.6 bits (56), Expect = 3.5
 Identities = 11/25 (44%), Positives = 16/25 (64%)
 Frame = +1

Query: 226 RQDVSAAISTRKELRKGSVPYPHET 300
           + +VSAA     EL   SVP+PH++
Sbjct: 320 KTNVSAAYEKPAELSSSSVPFPHKS 344


>SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 527

 Score = 26.2 bits (55), Expect = 4.6
 Identities = 9/21 (42%), Positives = 13/21 (61%)
 Frame = +1

Query: 145 RERPLTTFHCACTWCPTNMNS 207
           R RP +T H +CT+C   + S
Sbjct: 459 RSRPDSTSHASCTYCVARLRS 479


>SPAC688.07c |||sequence orphan|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1038

 Score = 25.8 bits (54), Expect = 6.1
 Identities = 11/20 (55%), Positives = 14/20 (70%)
 Frame = -2

Query: 403  STLRLAKRTTHPSLEPISSS 344
            +T +LAKR T PSL P  S+
Sbjct: 957  TTTKLAKRITQPSLSPARST 976


>SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 2685

 Score = 25.4 bits (53), Expect = 8.1
 Identities = 12/38 (31%), Positives = 19/38 (50%)
 Frame = -1

Query: 143 LTQIEDTDLGIRYTPTEPRFRIRFIFAVPVASCWPAPH 30
           L Q++++ L +  T T P   +R      V SC+P  H
Sbjct: 408 LHQLQNSILRVNSTLTSPSLDLRLELLHDVLSCFPKKH 445


>SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1234

 Score = 25.4 bits (53), Expect = 8.1
 Identities = 11/39 (28%), Positives = 21/39 (53%)
 Frame = -1

Query: 230  CLQSL*AQLFIFVGHQVHAQWKVVNGRSLLTQIEDTDLG 114
            CL  L   L   + H++ ++W+ +N R LL + +  + G
Sbjct: 940  CLLPLLPALLSELDHKLVSEWRRINDRGLLVEEDAEETG 978


>SPBC577.09 |||ERCC-8 homolog |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 404

 Score = 25.4 bits (53), Expect = 8.1
 Identities = 14/65 (21%), Positives = 28/65 (43%)
 Frame = +2

Query: 470 VIEALRRAKFKFPDVKRSTYQRSGVSQSMNVMSLRSCVKRAASLMTAALCSTARNMDLST 649
           +  A R +  +  D++  +Y  S    + NV+++  C K    L + +   T R  D+  
Sbjct: 162 IATAYRSSSIRLCDMQSGSYTHSLSGHTGNVLAVDWCPKNEFVLASGSADGTCRLWDIRK 221

Query: 650 LGGRF 664
           +   F
Sbjct: 222 VSSSF 226


>SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 3131

 Score = 25.4 bits (53), Expect = 8.1
 Identities = 14/35 (40%), Positives = 20/35 (57%)
 Frame = +2

Query: 272 KDQFHIRMRLHPFHVIRINKMLSCAGADRLQTGMR 376
           +D  H+RM ++ FH   IN MLS +  D  +  MR
Sbjct: 859 EDNSHLRMSINSFH---INDMLSKSSRDNNRQLMR 890


>SPCC24B10.07 |gad8||serine/threonine protein kinase Gad8
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 569

 Score = 25.4 bits (53), Expect = 8.1
 Identities = 20/75 (26%), Positives = 32/75 (42%), Gaps = 8/75 (10%)
 Frame = -3

Query: 246 CSRYVLPP-KP-LSSAVHIRRTPSARTVESRQRS------LSSYPNRRYGSWDQVHPDRT 91
           C++ + PP KP + SA+      S  T E    S      LS    +R+ +W    P   
Sbjct: 494 CAKKIQPPFKPSVESAIDTSNFDSEFTSEIPMDSVVADSHLSETVQQRFANWSYQRPTTI 553

Query: 90  SISDTVYFCSTGSIL 46
             SD +   + GS++
Sbjct: 554 DTSDDINTIAPGSVI 568


>SPAC3A12.05c |taf2||TATA-binding protein associated factor
           Taf2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1174

 Score = 25.4 bits (53), Expect = 8.1
 Identities = 8/15 (53%), Positives = 13/15 (86%)
 Frame = +1

Query: 97  VGVYLIPRSVSSIWV 141
           +GVYLIP++ S +W+
Sbjct: 394 IGVYLIPKAWSDLWL 408


>SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1957

 Score = 25.4 bits (53), Expect = 8.1
 Identities = 12/26 (46%), Positives = 16/26 (61%)
 Frame = +3

Query: 132  DLGKKRATVDDFPLCVHLVSDEYEQL 209
            ++ KKRA  DD    + +VS EYE L
Sbjct: 1033 EIEKKRAENDDLQSKLSVVSSEYENL 1058


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,199,149
Number of Sequences: 5004
Number of extensions: 69595
Number of successful extensions: 232
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 223
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 232
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 331187010
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -