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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00392
         (702 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g25280.1 68415.m03024 expressed protein                            119   2e-27
At1g17110.1 68414.m02085 ubiquitin-specific protease 15 (UBP15) ...    29   2.3  

>At2g25280.1 68415.m03024 expressed protein
          Length = 291

 Score =  119 bits (287), Expect = 2e-27
 Identities = 77/198 (38%), Positives = 110/198 (55%), Gaps = 7/198 (3%)
 Frame = +1

Query: 115 RQAYHAGCWYTENGSELSRQLDLWLSKADLTHGP-ARAIIAPHAGYSYAALARR---THI 282
           RQ  HAG WYT+N ++LS  L+ WL+   LT  P  R +IAPHAGYSY+  A      +I
Sbjct: 5   RQPTHAGSWYTDNPTKLSSDLEEWLNATGLTKSPDVRGVIAPHAGYSYSGRAAAYAFANI 64

Query: 283 DK---SVLWLLNESSYWAHHTT*GSQDALFRPSTSTRLPSTISPSINKYTRS*GHTSIR* 453
           D    S ++LL  S ++       S   +++      LP  +   + K  R+ G   +  
Sbjct: 65  DPTNISRIFLLGPSHHFYTPKCALSTATVYKTPIGN-LP--VDVEMIKEIRAMGKFGMM- 120

Query: 454 NGRADR*K*AFH*NAPTLLAKVMEEYKTSFTIIPILVGSLTPEKEAKYGAILAPYLADPQ 633
           + R D  + +   + P  LAKV E    +  ++PILVG+++PE EA YG +LA Y+ DP+
Sbjct: 121 DLRVDEAEHSMEMHLP-YLAKVFEG--NNVKVVPILVGAVSPENEAMYGELLAKYVDDPK 177

Query: 634 NLLVISSDFCHWGSRFRY 687
           N   +SSDFCHWGSRF Y
Sbjct: 178 NFFSVSSDFCHWGSRFNY 195



 Score = 60.9 bits (141), Expect = 8e-10
 Identities = 25/59 (42%), Positives = 38/59 (64%)
 Frame = +3

Query: 258 GACSAYAYRQVSPVVVKRIFILGPSHHVRIAGCALSSLDKYQTPLYDLTIDKQIYAELR 434
           G  +AYA+  + P  + RIF+LGPSHH     CALS+   Y+TP+ +L +D ++  E+R
Sbjct: 54  GRAAAYAFANIDPTNISRIFLLGPSHHFYTPKCALSTATVYKTPIGNLPVDVEMIKEIR 112



 Score = 36.7 bits (81), Expect = 0.015
 Identities = 16/30 (53%), Positives = 20/30 (66%)
 Frame = +2

Query: 419 IRGAEATRQFDRMDEQTDENEHSIEMHLPY 508
           I+   A  +F  MD + DE EHS+EMHLPY
Sbjct: 108 IKEIRAMGKFGMMDLRVDEAEHSMEMHLPY 137


>At1g17110.1 68414.m02085 ubiquitin-specific protease 15 (UBP15)
           almost identical to ubiquitin-specific protease 15
           GI:11993475 [Arabidopsis thaliana], 7 amino acid
           difference
          Length = 924

 Score = 29.5 bits (63), Expect = 2.3
 Identities = 16/49 (32%), Positives = 22/49 (44%)
 Frame = -3

Query: 484 MLIFIGLLVHSIELTCGLSSAYICLSMVRS*RGVWYLSRDERAHPAILT 338
           + +   ++VH   L    S  YI  S V+  RG WY   D   HP  +T
Sbjct: 683 LYMLYAVIVHLDTLNASFSGHYI--SYVKDLRGNWYRIDDSEIHPVPMT 729


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,446,171
Number of Sequences: 28952
Number of extensions: 319895
Number of successful extensions: 837
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 813
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 836
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1506636208
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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