SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00391
         (639 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p...   109   3e-25
SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ...   108   8e-25
SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc...    94   1e-20
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M...    92   5e-20
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce...    73   4e-14
SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe...    54   1e-08
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch...    46   5e-06
SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom...    31   0.11 
SPAC9.10 |||amino acid permease, unknown 2|Schizosaccharomyces p...    27   3.0  
SPAC24B11.10c |chr3|cfh1|chitin synthase regulatory factor Chr3 ...    26   4.0  
SPBC16G5.16 |||transcription factor zf-fungal binuclear cluster ...    26   4.0  
SPCC584.05 |sec1||SNARE binding protein Sec1|Schizosaccharomyces...    26   5.3  
SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S...    25   9.2  
SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|ch...    25   9.2  
SPBC16C6.05 |||translation initiation factor |Schizosaccharomyce...    25   9.2  
SPBC18E5.01 ||SPBC29A3.19|cycloisomerase 2 family|Schizosaccharo...    25   9.2  
SPAC637.11 |suv3||ATP-dependent RNA helicase Suv3|Schizosaccharo...    25   9.2  
SPBC3B9.02c |cwf28||splicing factor Cwf28|Schizosaccharomyces po...    25   9.2  

>SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 647

 Score =  109 bits (262), Expect = 3e-25
 Identities = 54/73 (73%), Positives = 56/73 (76%)
 Frame = +1

Query: 295 GIETAGGVMTTLIKRNXXXXXXXXXXXXXYSDNQPGVLIQVFEGECAMTKDNNLLGKFEL 474
           GIETAGGVMT LIKRN             YSDNQPGVLIQVFEGE A TKD NLLGKFEL
Sbjct: 400 GIETAGGVMTPLIKRNTTIPTKKSEIFSTYSDNQPGVLIQVFEGERARTKDCNLLGKFEL 459

Query: 475 TGIPPAPRGVPQL 513
           +GIPPAPRGVPQ+
Sbjct: 460 SGIPPAPRGVPQI 472



 Score =  108 bits (259), Expect = 8e-25
 Identities = 52/81 (64%), Positives = 62/81 (76%)
 Frame = +2

Query: 11  ADLFRSTMEPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKSINPD 190
           ADLFR TMEPVE+ LRD+K+DKA +++IVLVGGSTRIP+VQKL+ DFFNGKE  KSINPD
Sbjct: 305 ADLFRKTMEPVERVLRDSKVDKASVNEIVLVGGSTRIPRVQKLVSDFFNGKEPCKSINPD 364

Query: 191 EXXXXXXXXXXXILHGDKSEE 253
           E           +L GD SE+
Sbjct: 365 EAVAYGAAVQAAVLTGDTSEK 385



 Score = 77.4 bits (182), Expect = 2e-15
 Identities = 36/43 (83%), Positives = 39/43 (90%)
 Frame = +3

Query: 510 IEVTFDIDANGILNVSAIEKSTNKENKITITNDKGRLSKEEIE 638
           IEVTFD+DANGILNVSA+EK T K  KITITNDKGRLSKEEI+
Sbjct: 472 IEVTFDVDANGILNVSALEKGTGKTQKITITNDKGRLSKEEID 514


>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 644

 Score =  108 bits (259), Expect = 8e-25
 Identities = 52/81 (64%), Positives = 63/81 (77%)
 Frame = +2

Query: 11  ADLFRSTMEPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKSINPD 190
           ADLFR+TMEPVEK LRD+K+DK+ +++IVLVGGSTRIP++QKL+ DFFNGKE  KSINPD
Sbjct: 305 ADLFRNTMEPVEKVLRDSKIDKSSVNEIVLVGGSTRIPRIQKLVSDFFNGKEPCKSINPD 364

Query: 191 EXXXXXXXXXXXILHGDKSEE 253
           E           IL GD SE+
Sbjct: 365 EAVAYGAAVQAAILVGDTSEK 385



 Score =  108 bits (259), Expect = 8e-25
 Identities = 53/73 (72%), Positives = 56/73 (76%)
 Frame = +1

Query: 295 GIETAGGVMTTLIKRNXXXXXXXXXXXXXYSDNQPGVLIQVFEGECAMTKDNNLLGKFEL 474
           GIETAGGVMT LIKRN             Y+DNQPGVLIQVFEGE A TKD NLLGKFEL
Sbjct: 400 GIETAGGVMTPLIKRNTTIPTKKSEVFSTYADNQPGVLIQVFEGERARTKDCNLLGKFEL 459

Query: 475 TGIPPAPRGVPQL 513
           +GIPPAPRGVPQ+
Sbjct: 460 SGIPPAPRGVPQI 472



 Score = 77.4 bits (182), Expect = 2e-15
 Identities = 36/43 (83%), Positives = 39/43 (90%)
 Frame = +3

Query: 510 IEVTFDIDANGILNVSAIEKSTNKENKITITNDKGRLSKEEIE 638
           IEVTFD+DANGILNVSA+EK T K  KITITNDKGRLSKEEI+
Sbjct: 472 IEVTFDVDANGILNVSALEKGTGKTQKITITNDKGRLSKEEID 514


>SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 613

 Score = 94.3 bits (224), Expect = 1e-20
 Identities = 41/62 (66%), Positives = 54/62 (87%)
 Frame = +2

Query: 8   SADLFRSTMEPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKSINP 187
           +A  F++T++PV K L+D+K+ KA +HDIVLVGGSTRIPKVQ+L+ DFF+G+ LNKSINP
Sbjct: 308 NATTFKATIDPVAKVLKDSKVPKADVHDIVLVGGSTRIPKVQRLVSDFFDGRALNKSINP 367

Query: 188 DE 193
           DE
Sbjct: 368 DE 369



 Score = 52.4 bits (120), Expect = 5e-08
 Identities = 23/46 (50%), Positives = 33/46 (71%)
 Frame = +3

Query: 501 RASIEVTFDIDANGILNVSAIEKSTNKENKITITNDKGRLSKEEIE 638
           +A +E TF++DANGIL V+A+EK+T +   I ITN  G LS  +I+
Sbjct: 474 QAELEATFELDANGILKVTAVEKTTGRSAHIEITNSVGHLSSTKIQ 519



 Score = 51.2 bits (117), Expect = 1e-07
 Identities = 26/73 (35%), Positives = 35/73 (47%)
 Frame = +1

Query: 295 GIETAGGVMTTLIKRNXXXXXXXXXXXXXYSDNQPGVLIQVFEGECAMTKDNNLLGKFEL 474
           G+   G V   +  RN              +DNQ  V   V++GE     +N  LG+F+L
Sbjct: 405 GVAMEGNVFGVVCPRNTPIPTIKKRTFTTVADNQTTVTFPVYQGERVNCAENEPLGEFQL 464

Query: 475 TGIPPAPRGVPQL 513
           TGIPP PRG  +L
Sbjct: 465 TGIPPMPRGQAEL 477


>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 663

 Score = 92.3 bits (219), Expect = 5e-20
 Identities = 46/73 (63%), Positives = 50/73 (68%)
 Frame = +1

Query: 295 GIETAGGVMTTLIKRNXXXXXXXXXXXXXYSDNQPGVLIQVFEGECAMTKDNNLLGKFEL 474
           GIET GGVMT LI RN               DNQ  VLIQV+EGE  +TKDNNLLGKF+L
Sbjct: 429 GIETTGGVMTKLIGRNTPIPTRKSQIFSTAVDNQNTVLIQVYEGERTLTKDNNLLGKFDL 488

Query: 475 TGIPPAPRGVPQL 513
            GIPPAPRGVPQ+
Sbjct: 489 RGIPPAPRGVPQI 501



 Score = 85.4 bits (202), Expect = 6e-18
 Identities = 40/80 (50%), Positives = 57/80 (71%)
 Frame = +2

Query: 14  DLFRSTMEPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKSINPDE 193
           DLF+ T++PVE+ L+D+ + K++I DIVLVGGSTRIPKVQ+LL+ FF GK+ +K INPDE
Sbjct: 338 DLFKKTLKPVEQVLKDSNLKKSEIDDIVLVGGSTRIPKVQELLESFF-GKKASKGINPDE 396

Query: 194 XXXXXXXXXXXILHGDKSEE 253
                      +L G++  +
Sbjct: 397 AVAYGAAVQAGVLSGEEGSD 416



 Score = 60.1 bits (139), Expect = 3e-10
 Identities = 28/44 (63%), Positives = 36/44 (81%), Gaps = 1/44 (2%)
 Frame = +3

Query: 510 IEVTFDIDANGILNVSAIEKS-TNKENKITITNDKGRLSKEEIE 638
           IEVTF++DANG+L VSA++KS   K  K+ I NDKGRLS+E+IE
Sbjct: 501 IEVTFEVDANGVLTVSAVDKSGKGKPEKLVIKNDKGRLSEEDIE 544


>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 674

 Score = 72.9 bits (171), Expect = 4e-14
 Identities = 35/73 (47%), Positives = 44/73 (60%)
 Frame = +1

Query: 295 GIETAGGVMTTLIKRNXXXXXXXXXXXXXYSDNQPGVLIQVFEGECAMTKDNNLLGKFEL 474
           GIET GGV T LI RN              +D Q  V I+VF+GE  + +DN L+G F+L
Sbjct: 443 GIETLGGVFTRLINRNTTIPTRKSQVFSTAADGQTAVEIRVFQGERELVRDNKLIGNFQL 502

Query: 475 TGIPPAPRGVPQL 513
           TGI PAP+G PQ+
Sbjct: 503 TGIAPAPKGQPQI 515



 Score = 58.4 bits (135), Expect = 8e-10
 Identities = 26/74 (35%), Positives = 47/74 (63%)
 Frame = +2

Query: 17  LFRSTMEPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKSINPDEX 196
           L R T++P +++L+DA +  ++I++++LVGG TR+P+V + ++  F  +E  KS+NPDE 
Sbjct: 355 LVRRTIDPCKRALKDANLQTSEINEVILVGGMTRMPRVVETVKSIFK-REPAKSVNPDEA 413

Query: 197 XXXXXXXXXXILHG 238
                     +L G
Sbjct: 414 VAIGAAIQGGVLSG 427



 Score = 52.0 bits (119), Expect = 7e-08
 Identities = 24/43 (55%), Positives = 34/43 (79%)
 Frame = +3

Query: 510 IEVTFDIDANGILNVSAIEKSTNKENKITITNDKGRLSKEEIE 638
           IEV+FD+DA+GI+NVSA +K+TNK++ IT+    G L+  EIE
Sbjct: 515 IEVSFDVDADGIINVSARDKATNKDSSITVAGSSG-LTDSEIE 556


>SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 848

 Score = 54.4 bits (125), Expect = 1e-08
 Identities = 21/60 (35%), Positives = 42/60 (70%)
 Frame = +2

Query: 14  DLFRSTMEPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKSINPDE 193
           D+  + +EP+ K+L+ A +  ++I+ I+L GG++RIP +Q  L D+ +  +++K++N DE
Sbjct: 342 DMEDAAVEPINKALKKANLTFSEINSIILFGGASRIPFIQSTLADYVSSDKISKNVNADE 401


>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
           Pss1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 720

 Score = 46.0 bits (104), Expect = 5e-06
 Identities = 21/52 (40%), Positives = 37/52 (71%)
 Frame = +2

Query: 38  PVEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKSINPDE 193
           P+EK+L  A + K  ++ I +VGG TR+P V++++ ++F GK L+ ++N DE
Sbjct: 322 PIEKALELAGIKKEDLYSIEMVGGCTRVPIVKEVIANYF-GKGLSFTLNQDE 372


>SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 566

 Score = 31.5 bits (68), Expect = 0.11
 Identities = 12/46 (26%), Positives = 27/46 (58%)
 Frame = +2

Query: 41  VEKSLRDAKMDKAQIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKS 178
           V +++  A M+   I +++L GG++  PK+  L++  F  + + +S
Sbjct: 341 VTEAVEKANMEPFDISEVILAGGASNTPKLTSLMESIFPEQTIIRS 386


>SPAC9.10 |||amino acid permease, unknown 2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 591

 Score = 26.6 bits (56), Expect = 3.0
 Identities = 14/36 (38%), Positives = 19/36 (52%), Gaps = 4/36 (11%)
 Frame = +3

Query: 9   APICSGLPWSQ----WRSLSVMPRWIRLKSTILYWW 104
           A ICS LP +     W + S  PR+ R  S ++ WW
Sbjct: 131 AEICSALPAAGSIYLWAAESAGPRFGRFVSFLVAWW 166


>SPAC24B11.10c |chr3|cfh1|chitin synthase regulatory factor Chr3
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 932

 Score = 26.2 bits (55), Expect = 4.0
 Identities = 9/19 (47%), Positives = 14/19 (73%)
 Frame = -2

Query: 89  RGFEPYPSWHHGETSPLAP 33
           +  +P P+  HG+TSPL+P
Sbjct: 420 KAIKPMPTSIHGQTSPLSP 438


>SPBC16G5.16 |||transcription factor zf-fungal binuclear cluster
           type |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 827

 Score = 26.2 bits (55), Expect = 4.0
 Identities = 13/28 (46%), Positives = 14/28 (50%)
 Frame = +2

Query: 341 TLPSPLNRLRHSPPTLITNPEYSSKYLR 424
           T   P  RLRH+P   I   E   KYLR
Sbjct: 43  TYGQPFKRLRHAPEKYIEFLELRLKYLR 70


>SPCC584.05 |sec1||SNARE binding protein Sec1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 693

 Score = 25.8 bits (54), Expect = 5.3
 Identities = 16/56 (28%), Positives = 26/56 (46%)
 Frame = -1

Query: 411 DEYSGLVIRVGGECLSLFSGDGSVTLDECGHDTSSSLNTEGKGCYIKQQQILPSSD 244
           D+YS L + +  EC+++F       +     D S+  N EGK       ++LP  D
Sbjct: 343 DQYS-LHLTMAQECMNIFEKQQLNLIGAIEQDLSTGSNVEGKVPRSVLSELLPLLD 397


>SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase
           Mok11|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 2397

 Score = 25.0 bits (52), Expect = 9.2
 Identities = 10/20 (50%), Positives = 11/20 (55%)
 Frame = -1

Query: 285 GCYIKQQQILPSSDLSPCKI 226
           GCY       P+ DLSP KI
Sbjct: 522 GCYSGSSSTYPAIDLSPAKI 541


>SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 509

 Score = 25.0 bits (52), Expect = 9.2
 Identities = 11/32 (34%), Positives = 22/32 (68%)
 Frame = +3

Query: 537 NGILNVSAIEKSTNKENKITITNDKGRLSKEE 632
           +GIL+ + IEK  +K  K+ + N+ G++S ++
Sbjct: 154 DGILSFTVIEKVDDKNLKVRV-NNNGKISSKK 184


>SPBC16C6.05 |||translation initiation factor |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 190

 Score = 25.0 bits (52), Expect = 9.2
 Identities = 11/34 (32%), Positives = 18/34 (52%)
 Frame = +3

Query: 531 DANGILNVSAIEKSTNKENKITITNDKGRLSKEE 632
           D    LNVS  + S  +E    +T ++ R+ +EE
Sbjct: 58  DLENTLNVSGTKDSNAEEQPAKLTKEEKRVEREE 91


>SPBC18E5.01 ||SPBC29A3.19|cycloisomerase 2
           family|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 342

 Score = 25.0 bits (52), Expect = 9.2
 Identities = 12/41 (29%), Positives = 18/41 (43%)
 Frame = -1

Query: 486 WDPGQLEFTEQVVIFGHSTLTLKYLDEYSGLVIRVGGECLS 364
           WD G+    ++  I GHS  +L  L+  S L        +S
Sbjct: 61  WDNGKFSLVDRFTIDGHSPTSLCVLENGSVLTANYNSASIS 101


>SPAC637.11 |suv3||ATP-dependent RNA helicase
           Suv3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 647

 Score = 25.0 bits (52), Expect = 9.2
 Identities = 14/41 (34%), Positives = 22/41 (53%)
 Frame = -3

Query: 592 ILFSLLVDFSIAETLRIPLASMSKVTSIEARHAAPVGSRSA 470
           I+FS L  FS   T+ IP+  + ++     RH  P GS+ +
Sbjct: 409 IVFSDLKKFSGVSTIDIPVPQIKQIAGRAGRH-NPNGSKQS 448


>SPBC3B9.02c |cwf28||splicing factor Cwf28|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 381

 Score = 25.0 bits (52), Expect = 9.2
 Identities = 12/32 (37%), Positives = 15/32 (46%), Gaps = 1/32 (3%)
 Frame = +2

Query: 539 RYPQRFRYREVHQQGEQDHHYQRQRSS-LQGR 631
           R P R +YRE H +  + H   R      QGR
Sbjct: 320 RDPDRTKYREYHSERRKQHRTDRYSDDYYQGR 351


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,578,847
Number of Sequences: 5004
Number of extensions: 51777
Number of successful extensions: 219
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 197
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 216
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 285732116
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -