Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00383
         (477 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...    99   2e-22
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...    65   5e-12
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...    64   1e-11
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    58   9e-10
SPBC1A4.10c |pmc1|SPBP23A10.01c, med14|mediator complex subunit ...    29   0.28 
SPBC19G7.16 |iws1||transcription elongation factor complex subun...    27   1.5  
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos...    26   2.6  
SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr 3|||Ma...    25   4.5  
SPAC23C4.15 |rpb5||DNA-directed RNA polymerase I, II and III sub...    25   7.9  

>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =   99 bits (238), Expect = 2e-22
 Identities = 41/57 (71%), Positives = 48/57 (84%)
 Frame = +1

Query: 82  MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGG 252
           MREIVHIQAGQCGNQ+GA FW  I+DEHG+D  G YHG S+ Q ER+NVY+NEA+GG
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGG 57



 Score = 93.5 bits (222), Expect = 1e-20
 Identities = 38/50 (76%), Positives = 45/50 (90%)
 Frame = +3

Query: 252 QYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFXQSGAGNNWAKGHYT 401
           +YVPRA+LVDLEPGTMD+V+SG FG +FRPDN ++ QSGAGN WAKGHYT
Sbjct: 58  KYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYT 107



 Score = 44.8 bits (101), Expect = 7e-06
 Identities = 19/25 (76%), Positives = 23/25 (92%)
 Frame = +2

Query: 401 EGAELVDSVLEVVRKEAESCDCLQG 475
           EGAEL D+VL+VVR+EAE+CD LQG
Sbjct: 108 EGAELADAVLDVVRREAEACDALQG 132


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score = 65.3 bits (152), Expect = 5e-12
 Identities = 25/50 (50%), Positives = 36/50 (72%)
 Frame = +3

Query: 252 QYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFXQSGAGNNWAKGHYT 401
           +YVPR+I VDLEP  +D VR+GP+  +F P+  +  +  A NN+A+GHYT
Sbjct: 60  KYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYT 109



 Score = 44.4 bits (100), Expect = 9e-06
 Identities = 19/34 (55%), Positives = 21/34 (61%)
 Frame = +1

Query: 82  MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG 183
           MREI+ I  GQ G QIG   WE+   EHGI P G
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNG 34



 Score = 29.1 bits (62), Expect = 0.37
 Identities = 12/24 (50%), Positives = 17/24 (70%)
 Frame = +2

Query: 404 GAELVDSVLEVVRKEAESCDCLQG 475
           G ELVD V + +R+ A++C  LQG
Sbjct: 111 GKELVDEVTDKIRRIADNCSGLQG 134


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score = 64.1 bits (149), Expect = 1e-11
 Identities = 25/50 (50%), Positives = 36/50 (72%)
 Frame = +3

Query: 252 QYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFXQSGAGNNWAKGHYT 401
           ++VPR+I VDLEP  +D VR+GP+  +F P+  V  +  A NN+A+GHYT
Sbjct: 64  KFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYT 113



 Score = 44.4 bits (100), Expect = 9e-06
 Identities = 22/56 (39%), Positives = 31/56 (55%)
 Frame = +1

Query: 82  MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASG 249
           MRE++ +  GQ G QIG   WE+   EHGI P G    +S  ++ + N Y N+  G
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENS--EVHKNNSYLNDGFG 54



 Score = 32.7 bits (71), Expect = 0.030
 Identities = 13/24 (54%), Positives = 19/24 (79%)
 Frame = +2

Query: 404 GAELVDSVLEVVRKEAESCDCLQG 475
           G E++DSVLE +R+ A++C  LQG
Sbjct: 115 GKEMIDSVLERIRRMADNCSGLQG 138


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 57.6 bits (133), Expect = 9e-10
 Identities = 22/50 (44%), Positives = 37/50 (74%), Gaps = 2/50 (4%)
 Frame = +3

Query: 252 QYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFXQS--GAGNNWAKGH 395
           +Y+PRAIL+DLEP  ++++ S  +G ++ P+N +  ++  GAGNNWA G+
Sbjct: 59  RYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANGY 108



 Score = 56.4 bits (130), Expect = 2e-09
 Identities = 23/53 (43%), Positives = 37/53 (69%)
 Frame = +1

Query: 85  REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEA 243
           REI+ +QAGQCGNQIG++FW+ +  EHGI P G     +   ++R +V++ ++
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQS 55


>SPBC1A4.10c |pmc1|SPBP23A10.01c, med14|mediator complex subunit
           Pmc1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 879

 Score = 29.5 bits (63), Expect = 0.28
 Identities = 14/42 (33%), Positives = 29/42 (69%)
 Frame = -1

Query: 129 DLVAALTSLDMYDFPHFVLFM*TINYLTLKMLLRTLHSLSLV 4
           D+++  TSL + + P   L+M + + L+ K +L+TLH+L+++
Sbjct: 123 DILSTGTSLRLANAPTSKLYMLSESPLSTKQILQTLHALNML 164


>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
           Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 428

 Score = 27.1 bits (57), Expect = 1.5
 Identities = 10/34 (29%), Positives = 22/34 (64%)
 Frame = +1

Query: 121 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 222
           N++G    E+++++  +DPT A   + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166


>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
           Pof11|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 506

 Score = 26.2 bits (55), Expect = 2.6
 Identities = 10/25 (40%), Positives = 15/25 (60%), Gaps = 1/25 (4%)
 Frame = +3

Query: 315 GPFGQIFRPDNFVFXQSGAG-NNWA 386
           GP+G +F P  F+F  +G    NW+
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWS 181


>SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 630

 Score = 25.4 bits (53), Expect = 4.5
 Identities = 18/66 (27%), Positives = 34/66 (51%), Gaps = 3/66 (4%)
 Frame = -3

Query: 475 SLEAITRFRFL---SNDF*N*IDELSTLV*CPLAQLLPAPDCXKTKLSGRKICPKGPERT 305
           ++ A++R  FL   +ND    +DEL++L     ++ L   D  +     ++ICP+G    
Sbjct: 185 TIHALSRIFFLIDKNNDDLLSVDELNSLSEKCFSKNLSIEDASEILSKVKEICPEGVYEG 244

Query: 304 ESMVPG 287
           +  +PG
Sbjct: 245 QLTLPG 250


>SPAC23C4.15 |rpb5||DNA-directed RNA polymerase I, II and III
           subunit Rpb5 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 210

 Score = 24.6 bits (51), Expect = 7.9
 Identities = 14/35 (40%), Positives = 16/35 (45%)
 Frame = -2

Query: 419 RRAQHPRVVSLGPVVAGAGLSEDEVVRTEDLSERS 315
           R  Q PR+    PV    GL   EVV+    SE S
Sbjct: 166 RETQLPRIQLADPVARYLGLKRGEVVKIVRRSETS 200


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,769,225
Number of Sequences: 5004
Number of extensions: 31405
Number of successful extensions: 103
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 92
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 102
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 184476110
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)