Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00381
(704 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g57620.1 68414.m06539 emp24/gp25L/p24 family protein similar ... 64 9e-11
At1g21900.1 68414.m02741 emp24/gp25L/p24 family protein similar ... 61 6e-10
At1g09580.1 68414.m01075 emp24/gp25L/p24 family protein similar ... 59 2e-09
At3g10780.1 68416.m01298 emp24/gp25L/p24 family protein similar ... 55 4e-08
At3g29070.1 68416.m03636 emp24/gp25L/p24 family protein similar ... 54 7e-08
At1g69460.1 68414.m07981 emp24/gp25L/p24 family protein similar ... 50 2e-06
At1g26690.1 68414.m03251 emp24/gp25L/p24 family protein similar ... 50 2e-06
At2g03290.1 68415.m00284 emp24/gp25L/p24 family protein similar ... 47 1e-05
At1g14010.1 68414.m01654 emp24/gp25L/p24 family protein similar ... 44 7e-05
At3g22845.1 68416.m02879 emp24/gp25L/p24 protein-related contain... 40 0.002
At2g03040.1 68415.m00257 transmembrane protein-related low simil... 29 3.0
At4g02500.1 68417.m00341 galactosyl transferase GMA12/MNN10 fami... 28 6.9
At3g26710.1 68416.m03340 expressed protein 28 6.9
At5g18530.1 68418.m02191 beige/BEACH domain-containing protein c... 27 9.2
At4g13050.1 68417.m02036 acyl-[acyl carrier protein] thioesteras... 27 9.2
>At1g57620.1 68414.m06539 emp24/gp25L/p24 family protein similar to
SP|P49755 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Homo sapiens};
contains Pfam profile PF01105: emp24/gp25L/p24 family
Length = 212
Score = 64.1 bits (149), Expect = 9e-11
Identities = 37/122 (30%), Positives = 63/122 (51%), Gaps = 1/122 (0%)
Frame = +3
Query: 309 GHILSQKDTVTKGKFSFVTENYDMFEVCFISKVPSERRGIPHQVSLDIKIGIEAKTYEGI 488
G +L ++ T G+F+F T+ + CF + S +++D K GI AK ++ I
Sbjct: 74 GTVLHHRENTTNGQFAFTTQESGTYLACFEADAKSHGNK-DFSINIDWKTGIAAKDWDSI 132
Query: 489 GEAAKLNQWKWN*RD*KTYSEAIVQDFTLMRKREEEMRDTNESTNNRVLFFSIFSMA-CL 665
K+ + + + EAI ++ +R RE EMR +E TN+RV ++SI S+ C+
Sbjct: 133 ARKEKIEGVELEFKKLEGAVEAIHENLIYLRNREAEMRIVSEKTNSRVAWYSIMSLGICI 192
Query: 666 WV 671
V
Sbjct: 193 VV 194
>At1g21900.1 68414.m02741 emp24/gp25L/p24 family protein similar to
SP|O35587 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Mesocricetus
auratus}; contains Pfam profile PF01105: emp24/gp25L/p24
family
Length = 216
Score = 61.3 bits (142), Expect = 6e-10
Identities = 35/116 (30%), Positives = 63/116 (54%)
Frame = +3
Query: 309 GHILSQKDTVTKGKFSFVTENYDMFEVCFISKVPSERRGIPHQVSLDIKIGIEAKTYEGI 488
G+ L ++ VT G+F+F T+ + CF S P + +D K+GI AK ++ +
Sbjct: 78 GNNLHHQENVTHGQFAFTTQEAGNYLACFWID-SSHHLANPITLGVDWKMGIAAKDWDSV 136
Query: 489 GEAAKLNQWKWN*RD*KTYSEAIVQDFTLMRKREEEMRDTNESTNNRVLFFSIFSM 656
+ K+ + R + +I ++ ++ RE EMR+ +E+TN+RV +FSI S+
Sbjct: 137 AKKEKIEGVELQLRRLEGLVLSIRENLNYIKDREAEMREVSETTNSRVAWFSIMSL 192
>At1g09580.1 68414.m01075 emp24/gp25L/p24 family protein similar to
SP|P49755 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Homo sapiens};
contains Pfam profile PF01105: emp24/gp25L/p24 family
Length = 217
Score = 59.3 bits (137), Expect = 2e-09
Identities = 37/123 (30%), Positives = 65/123 (52%), Gaps = 2/123 (1%)
Frame = +3
Query: 309 GHILSQKDTVTKGKFSFVTENYDMFEVCFISKVPSE-RRGIPHQVSLDIKIGIEAKTYEG 485
G+ L + VT G+F+F T+ + CF + S + + +++D + GI AK +
Sbjct: 79 GNNLHNMENVTHGQFAFTTQESGNYLACFWADEKSHGNKNV--SINIDWRTGIAAKDWAS 136
Query: 486 IGEAAKLNQWKWN*RD*KTYSEAIVQDFTLMRKREEEMRDTNESTNNRVLFFSIFSM-AC 662
I + K+ + R + EAI ++ +R RE +MR +E TN+RV ++SI S+ C
Sbjct: 137 IAKKEKIEGVELEIRKLEGAVEAIHENILYLRNREADMRTMSEKTNSRVAWYSIMSLGVC 196
Query: 663 LWV 671
+ V
Sbjct: 197 IAV 199
>At3g10780.1 68416.m01298 emp24/gp25L/p24 family protein similar to
SP|O35587 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Mesocricetus
auratus}; contains Pfam profile: PF01105 emp24/gp25L/p24
family; contains non-consensus TG acceptor splice site
at exon 3
Length = 217
Score = 55.2 bits (127), Expect = 4e-08
Identities = 37/130 (28%), Positives = 56/130 (43%), Gaps = 2/130 (1%)
Frame = +3
Query: 273 GQRVDYIIKDSKGHILSQKDTVTKGKFSFVTENYDMFEVCFISKVPSERRGIPHQV--SL 446
G +D + G L + VT G+ +F T F C + V SL
Sbjct: 64 GPTLDVRVTSPYGKELYKIANVTHGQAAFTTSESGTFLACLAMHHDQSHHSVNSSVIVSL 123
Query: 447 DIKIGIEAKTYEGIGEAAKLNQWKWN*RD*KTYSEAIVQDFTLMRKREEEMRDTNESTNN 626
D K+GI AK ++ + + K+ + R Y+ AI + +R RE MR+ NE TN
Sbjct: 124 DWKMGIRAKDWDSVAKKEKIEAMELEIRRSTEYASAIRANILYLRIREAYMREINEKTNT 183
Query: 627 RVLFFSIFSM 656
RV + S+
Sbjct: 184 RVNQLGLMSL 193
>At3g29070.1 68416.m03636 emp24/gp25L/p24 family protein similar to
SP|Q28735 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Oryctolagus
cuniculus}; contains Pfam profile: PF01105
emp24/gp25L/p24 family
Length = 204
Score = 54.4 bits (125), Expect = 7e-08
Identities = 33/120 (27%), Positives = 55/120 (45%)
Frame = +3
Query: 294 IKDSKGHILSQKDTVTKGKFSFVTENYDMFEVCFISKVPSERRGIPHQVSLDIKIGIEAK 473
+ KG + V GKF F E + CF++ P R V + K G+EAK
Sbjct: 62 VSSPKGKSHHHAENVESGKFVFTAEETGDYMTCFVA--PGYRPTAKFAVDFEWKSGVEAK 119
Query: 474 TYEGIGEAAKLNQWKWN*RD*KTYSEAIVQDFTLMRKREEEMRDTNESTNNRVLFFSIFS 653
+ I + ++ + R +E I ++ + +RE EM++ N STN+R+ S+ S
Sbjct: 120 DWTTIAKRGQITMLEVEVRKLLDVTETIHEEMFQLIEREREMQELNRSTNSRMAALSLLS 179
>At1g69460.1 68414.m07981 emp24/gp25L/p24 family protein similar to
SP|Q28735 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Oryctolagus
cuniculus}; contains Pfam profile: PF01105:
emp24/gp25L/p24 family
Length = 214
Score = 50.0 bits (114), Expect = 2e-06
Identities = 27/135 (20%), Positives = 64/135 (47%), Gaps = 1/135 (0%)
Frame = +3
Query: 270 QGQRVDYIIKDSKGHILSQKDTVTKGKFSFVTENYDMFEVCFISKVPSERRGIPHQVSLD 449
Q ++ + + G+ + V G+F+F + CF + + + + +
Sbjct: 64 QTHKISVKVTSNSGNNYHHAEQVDSGQFAFSAVEAGDYMACFTAV--DHKPEVSLSIDFE 121
Query: 450 IKIGIEAKTYEGIGEAAKLNQWKWN*RD*KTYSEAIVQDFTLMRKREEEMRDTNESTNNR 629
K G+++K++ + + +++ ++ + +I ++ +R REEEM+D N STN +
Sbjct: 122 WKTGVQSKSWANVAKKSQVEVMEFEVKSLLDTVNSIHEEMYYLRDREEEMQDLNRSTNTK 181
Query: 630 VLFFSIFS-MACLWV 671
+ + S+ S C+ V
Sbjct: 182 MAWLSVLSFFVCIGV 196
>At1g26690.1 68414.m03251 emp24/gp25L/p24 family protein similar to
SP|P49755 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Homo sapiens};
contains Pfam profile PF01105: emp24/gp25L/p24 family
Length = 214
Score = 50.0 bits (114), Expect = 2e-06
Identities = 26/135 (19%), Positives = 67/135 (49%), Gaps = 1/135 (0%)
Frame = +3
Query: 270 QGQRVDYIIKDSKGHILSQKDTVTKGKFSFVTENYDMFEVCFISKVPSERRGIPHQVSLD 449
Q ++ + S G+ + V G+F+F + C+ + + + + D
Sbjct: 64 QSHKISIRVTSSYGNTYHHAEDVESGQFAFTAVESGDYMACYTAV--DHKPEVTLSIDFD 121
Query: 450 IKIGIEAKTYEGIGEAAKLNQWKWN*RD*KTYSEAIVQDFTLMRKREEEMRDTNESTNNR 629
+ G+++K++ + + +++ +++ + +I ++ +R+REEEM++ N +TN++
Sbjct: 122 WRTGVQSKSWSSVAKKSQVEVMEFDVKRLIETVNSIHEEMFYLREREEEMQNLNRATNSK 181
Query: 630 VLFFSIFSM-ACLWV 671
+ + S S+ CL V
Sbjct: 182 MAWLSFLSLFVCLGV 196
>At2g03290.1 68415.m00284 emp24/gp25L/p24 family protein similar to
SP|P49755 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Homo sapiens};
contains Pfam profile PF01105: emp24/gp25L/p24 family
Length = 170
Score = 47.2 bits (107), Expect = 1e-05
Identities = 29/123 (23%), Positives = 60/123 (48%), Gaps = 1/123 (0%)
Frame = +3
Query: 306 KGHILSQKDTVTKGKFSFVTENYDMFEVCFISKVPSERRGIPHQVSLDIKIGIEAKTYEG 485
+G L + D V G+FSF + C + + + D K G+ +K +
Sbjct: 32 QGKNLHEADKVEAGQFSFTAYENGSYVACITAIDYKPETTLT--IDFDWKTGVHSKEWTN 89
Query: 486 IGEAAKLNQWKWN*RD*KTYSEAIVQDFTLMRKREEEMRDTNESTNNRVLFFSIFSM-AC 662
+ + ++++ ++ + +I ++ +R+REEEM++ N STN+++ + S S+ C
Sbjct: 90 VAKKSQVDMMEYQVKTLMDTVISIHEEMYYLREREEEMQELNRSTNSKMAWLSFGSLVVC 149
Query: 663 LWV 671
L V
Sbjct: 150 LSV 152
>At1g14010.1 68414.m01654 emp24/gp25L/p24 family protein similar to
SP|P49755 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Homo sapiens};
contains Pfam profile PF01105: emp24/gp25L/p24 family
Length = 212
Score = 44.4 bits (100), Expect = 7e-05
Identities = 31/132 (23%), Positives = 58/132 (43%), Gaps = 1/132 (0%)
Frame = +3
Query: 279 RVDYIIKDSKGHILSQKDTVTKGKFSFVTENYDMFEVCFISKVPSERRGIPHQVSLDIKI 458
+V + +G + D V G+FSFV + CF + + D +
Sbjct: 65 KVTVRVTSPQGTAYHESDGVESGQFSFVAVETGDYISCF--SAVDHKPETTLIIDFDWRT 122
Query: 459 GIEAKTYEGIGEAAKLNQWKWN*RD*KTYSEAIVQDFTLMRKREEEMRDTNESTNNRVLF 638
GI K + + + +++ ++ + I + +R REEEM + N +TN+++ +
Sbjct: 123 GIHTKDWSNVAKKSQVETMEFEVKKLFETVNGIHDEMFYLRDREEEMHNLNIATNSKMAW 182
Query: 639 FSIFSMA-CLWV 671
S S+A CL V
Sbjct: 183 LSFVSLAVCLSV 194
Score = 32.7 bits (71), Expect = 0.24
Identities = 14/40 (35%), Positives = 25/40 (62%)
Frame = +1
Query: 133 LVLLLSLIWHGTDAIMWSLAPNTHKCLKEELHANVLVAGE 252
++L+LS++ T +I + L KC+ EE+HAN + G+
Sbjct: 9 VLLILSILSPVTLSIRYELLSGHTKCISEEIHANAMTIGK 48
>At3g22845.1 68416.m02879 emp24/gp25L/p24 protein-related contains
weak similarity to transmembrane protein (GI:1212965)
[Homo sapiens]
Length = 214
Score = 39.5 bits (88), Expect = 0.002
Identities = 27/122 (22%), Positives = 55/122 (45%)
Frame = +3
Query: 282 VDYIIKDSKGHILSQKDTVTKGKFSFVTENYDMFEVCFISKVPSERRGIPHQVSLDIKIG 461
+D+ + G+I+ + KF F M++ CF + + P VS I +G
Sbjct: 69 LDFTVTSPAGNIVQTLKGTSGDKFEFKAPKSGMYKFCFHNPYST-----PETVSFYIHVG 123
Query: 462 IEAKTYEGIGEAAKLNQWKWN*RD*KTYSEAIVQDFTLMRKREEEMRDTNESTNNRVLFF 641
++ + + L+ + + E++V + ++ R+ R TNEST RV+F+
Sbjct: 124 HIPNEHD-LAKDEHLDPVNVKIAELREALESVVAEQKYLKARDTRHRHTNESTRKRVIFY 182
Query: 642 SI 647
++
Sbjct: 183 TV 184
>At2g03040.1 68415.m00257 transmembrane protein-related low
similarity to SP|Q28735|TM21_RABIT Transmembrane protein
Tmp21 precursor (21 kDa Transmembrane trafficking
protein) (Integral membrane protein p23) {Oryctolagus
cuniculus}
Length = 166
Score = 29.1 bits (62), Expect = 3.0
Identities = 12/40 (30%), Positives = 25/40 (62%)
Frame = +1
Query: 133 LVLLLSLIWHGTDAIMWSLAPNTHKCLKEELHANVLVAGE 252
L+L+++L+ T ++ + L + KC+ EE+H N + G+
Sbjct: 9 LLLIIALLSPRTLSMRYELKSSKTKCIGEEIHENAMSIGK 48
>At4g02500.1 68417.m00341 galactosyl transferase GMA12/MNN10 family
protein low similarity to
alpha-1,2-galactosyltransferase, Schizosaccharomyces
pombe [SP|Q09174]
Length = 461
Score = 27.9 bits (59), Expect = 6.9
Identities = 16/64 (25%), Positives = 33/64 (51%)
Frame = +3
Query: 327 KDTVTKGKFSFVTENYDMFEVCFISKVPSERRGIPHQVSLDIKIGIEAKTYEGIGEAAKL 506
+ T+ GKF ++ D F ++ +RG PH+V +I+ G ++ + +G G +
Sbjct: 36 RSTIGAGKFGTPEQDLDEIRQHFHAR----KRGEPHRVLEEIQTGGDSSSGDGGGNSGGS 91
Query: 507 NQWK 518
N ++
Sbjct: 92 NNYE 95
>At3g26710.1 68416.m03340 expressed protein
Length = 267
Score = 27.9 bits (59), Expect = 6.9
Identities = 14/34 (41%), Positives = 18/34 (52%)
Frame = -2
Query: 337 TVSFCDKMCPFESLII*STLCPCISVTSILLQLT 236
T++F KM P T C CIS+ S+ L LT
Sbjct: 151 TITFEGKMVPSRGQAALLTFCTCISLASVGLVLT 184
>At5g18530.1 68418.m02191 beige/BEACH domain-containing protein
contains 5 WD-40 repeats (PF00400); contains
Beige/BEACH domain (Pfam PF02138); FACTOR ASSOCIATED
WITH N-SMASE ACTIVATION (FAN) (SP:Q92636) Homo
sapiens;similar to Lipopolysaccharide-responsive and
beige-like anchor protein (CDC4-like protein)
(Beige-like protein) (SP:P50851) [Homo sapiens}
Length = 909
Score = 27.5 bits (58), Expect = 9.2
Identities = 11/33 (33%), Positives = 20/33 (60%)
Frame = -1
Query: 464 YADFNI*AYLMWNTSSF*WYFRYKANFKHIIIL 366
YAD I ++L W T W+ +NF+++++L
Sbjct: 333 YADLKISSHLDWQTHFDKWWKGELSNFEYLLVL 365
>At4g13050.1 68417.m02036 acyl-[acyl carrier protein]
thioesterase, putative / acyl-ACP thioesterase,
putative / oleoyl-[acyl-carrier protein] hydrolase,
putative / S-acyl fatty acid synthase thioesterase,
putative strong similarity to acyl-ACP thioesterase;
oleoyl-[acyl-carrier protein] hydrolase [Brassica
napus] GI:435011; contains Pfam profile PF01643:
Acyl-ACP thioesterase
Length = 367
Score = 27.5 bits (58), Expect = 9.2
Identities = 13/32 (40%), Positives = 16/32 (50%), Gaps = 1/32 (3%)
Frame = -1
Query: 95 LSCNK*SRIHNIYDRKTNI-ISNFTSNRPISC 3
LSCN IHN++ I + RPISC
Sbjct: 4 LSCNVTDHIHNLFSNSRRIFVPVHRQTRPISC 35
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,857,489
Number of Sequences: 28952
Number of extensions: 299270
Number of successful extensions: 650
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 618
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 643
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1516419560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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