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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00372
         (458 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase |Schizosaccharom...    83   1e-17
SPBC902.05c |idh2|glu2|isocitrate dehydrogenase |Schizosaccharom...    60   2e-10
SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces ...    36   0.004
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo...    26   2.4  
SPCC1919.10c |myo52||myosin type V|Schizosaccharomyces pombe|chr...    25   4.2  
SPAC15A10.03c |rhp54|rad54|Rad54 homolog Rhp54|Schizosaccharomyc...    25   4.2  
SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces po...    25   5.6  
SPBC2G2.02 |syj1||inositol-polyphosphate 5-phosphatase |Schizosa...    25   7.3  
SPCC24B10.10c |||mitochondrial outer membrane ATPase Msp1 |Schiz...    25   7.3  
SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase Shk1|Schizosacc...    24   9.7  
SPCC1682.09c |||guanine nucleotide transporter|Schizosaccharomyc...    24   9.7  

>SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 356

 Score = 83.4 bits (197), Expect = 1e-17
 Identities = 39/62 (62%), Positives = 49/62 (79%)
 Frame = +1

Query: 271 TSRNVALRNELDMYAYILNCKSYPGVATRHKDIDVVIIRQNTEGEYAMLEHESVNGVVES 450
           TS NVALR ELD+YA ++  K+ PG  TRH ++D  IIR+NTEGEY+ LEH+SV GVVES
Sbjct: 99  TSFNVALRKELDIYASLVLIKNIPGFKTRHDNVDFAIIRENTEGEYSGLEHQSVPGVVES 158

Query: 451 MK 456
           +K
Sbjct: 159 LK 160



 Score = 51.6 bits (118), Expect = 6e-08
 Identities = 28/82 (34%), Positives = 42/82 (51%), Gaps = 4/82 (4%)
 Frame = +3

Query: 12  KQKLIPKAQYGGRHAVTMLPGGGIGPECMGYVRDIFKYIGAPIDFEVVDIDPTMDND--- 182
           K       +YGG++ VT++PG GIG E    V +IFK    PI+FE +D+     N+   
Sbjct: 8   KSSAFQPLKYGGKYTVTLIPGDGIGRETSNAVTEIFKTANVPIEFEEIDVTGMEKNNKSS 67

Query: 183 -DDVQYAITTIKXTVWG*RATL 245
            D +  AI ++K    G +  L
Sbjct: 68  GDALHEAIQSLKRNKVGLKGIL 89


>SPBC902.05c |idh2|glu2|isocitrate dehydrogenase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 378

 Score = 60.1 bits (139), Expect = 2e-10
 Identities = 26/61 (42%), Positives = 41/61 (67%)
 Frame = +1

Query: 274 SRNVALRNELDMYAYILNCKSYPGVATRHKDIDVVIIRQNTEGEYAMLEHESVNGVVESM 453
           S N+ LR    ++A +  C S  G  T + +++ V+IR+NTEGEY+ +EHE + GVV+S+
Sbjct: 122 SMNLTLRRTFGLFANVRPCVSITGYKTPYDNVNTVLIRENTEGEYSGIEHEVIPGVVQSI 181

Query: 454 K 456
           K
Sbjct: 182 K 182



 Score = 42.3 bits (95), Expect = 3e-05
 Identities = 26/71 (36%), Positives = 36/71 (50%), Gaps = 5/71 (7%)
 Frame = +3

Query: 45  GRHAVTMLPGGGIGPECMGYVRDIFKYIGAPIDFEVVDIDPTMDN-----DDDVQYAITT 209
           G + VTM+ G GIGPE    V  IFK    PI++E V + P + N      DD + ++  
Sbjct: 44  GNYTVTMIAGDGIGPEIAQSVERIFKAAKVPIEWERVKVYPILKNGTTTIPDDAKESVRK 103

Query: 210 IKXTVWG*RAT 242
            K  + G  AT
Sbjct: 104 NKVALKGPLAT 114


>SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 362

 Score = 35.5 bits (78), Expect = 0.004
 Identities = 19/46 (41%), Positives = 28/46 (60%)
 Frame = +1

Query: 283 VALRNELDMYAYILNCKSYPGVATRHKDIDVVIIRQNTEGEYAMLE 420
           VALR ++ +YA +   KS  G   + K +D+VI+R+NTE  Y   E
Sbjct: 94  VALRKKMGLYANVRPVKSLDGA--KGKPVDLVIVRENTECLYVKEE 137


>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1224

 Score = 26.2 bits (55), Expect = 2.4
 Identities = 15/46 (32%), Positives = 23/46 (50%)
 Frame = -2

Query: 190 TSSSLSIVGSMSTTSKSIGAPMYLNMSRTYPMHSGPIPPPGSMVTA 53
           TS + SI+ ++  T+ S  +P     S   P    P+PPP +  TA
Sbjct: 816 TSQASSILPTVPRTT-SYTSPYATTSSHITPADVHPLPPPSTSTTA 860


>SPCC1919.10c |myo52||myosin type V|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1516

 Score = 25.4 bits (53), Expect = 4.2
 Identities = 16/58 (27%), Positives = 29/58 (50%), Gaps = 5/58 (8%)
 Frame = +1

Query: 292 RNELDMYAYILNCKSYPGVATRHKDIDVVIIRQNTEGE-----YAMLEHESVNGVVES 450
           R+ELD + Y +   SY  +   HK+  ++I  ++  G+     Y M    SV  +++S
Sbjct: 136 RDELDPHLYAIAEDSYKCMNQEHKNQTIIISGESGAGKTVSARYIMRYFASVQALIQS 193


>SPAC15A10.03c |rhp54|rad54|Rad54 homolog Rhp54|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 852

 Score = 25.4 bits (53), Expect = 4.2
 Identities = 10/34 (29%), Positives = 20/34 (58%)
 Frame = -1

Query: 215 LNGRYSILDIIIIVHRWVNVHHFEVNRSTDVFEY 114
           L+G+ S  ++I+ + +W +VH  +V R   +  Y
Sbjct: 356 LDGKSSKQELIMALQQWASVHGRQVTRPVLIASY 389


>SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 981

 Score = 25.0 bits (52), Expect = 5.6
 Identities = 14/31 (45%), Positives = 17/31 (54%), Gaps = 1/31 (3%)
 Frame = +1

Query: 364 DIDVVIIRQNTEGEYAMLEHES-VNGVVESM 453
           DID   IR   E E A++ H S   GV +SM
Sbjct: 148 DIDAPCIRAGLEPEVALINHSSGSTGVPKSM 178


>SPBC2G2.02 |syj1||inositol-polyphosphate 5-phosphatase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1076

 Score = 24.6 bits (51), Expect = 7.3
 Identities = 11/28 (39%), Positives = 18/28 (64%)
 Frame = -2

Query: 199 AYWTSSSLSIVGSMSTTSKSIGAPMYLN 116
           ++     LSI G+++  SKS+G  MY+N
Sbjct: 481 SFTRKGKLSIAGALNDLSKSVGR-MYIN 507


>SPCC24B10.10c |||mitochondrial outer membrane ATPase Msp1
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 355

 Score = 24.6 bits (51), Expect = 7.3
 Identities = 12/31 (38%), Positives = 17/31 (54%)
 Frame = +1

Query: 64  CSLEAVSVLSAWDTFATYSNTSVLLLTSKWW 156
           C    ++   A  + AT+ N SV LLT KW+
Sbjct: 136 CGKTMLAKALAKQSQATFINVSVGLLTDKWF 166


>SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase
           Shk1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 658

 Score = 24.2 bits (50), Expect = 9.7
 Identities = 13/42 (30%), Positives = 18/42 (42%)
 Frame = -2

Query: 190 TSSSLSIVGSMSTTSKSIGAPMYLNMSRTYPMHSGPIPPPGS 65
           T   L  V ++S++S         + SR YP    P PP  S
Sbjct: 223 TEKPLLSVSALSSSSHLQPTSATSSSSRLYPSRPAPTPPASS 264


>SPCC1682.09c |||guanine nucleotide transporter|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 300

 Score = 24.2 bits (50), Expect = 9.7
 Identities = 12/29 (41%), Positives = 16/29 (55%)
 Frame = +3

Query: 54  AVTMLPGGGIGPECMGYVRDIFKYIGAPI 140
           A ++ PG G    C   V+ I+KY G PI
Sbjct: 70  ATSLFPGLGYAA-CYKIVQRIYKYSGQPI 97


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,964,491
Number of Sequences: 5004
Number of extensions: 38289
Number of successful extensions: 135
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 129
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 134
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 172312850
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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