Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00372
(458 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g35260.1 68417.m05011 isocitrate dehydrogenase subunit 1 / NA... 89 1e-18
At2g17130.2 68415.m01978 isocitrate dehydrogenase subunit 2 / NA... 89 1e-18
At2g17130.1 68415.m01977 isocitrate dehydrogenase subunit 2 / NA... 89 1e-18
At4g35650.1 68417.m05062 isocitrate dehydrogenase, putative / NA... 87 5e-18
At5g03290.1 68418.m00279 isocitrate dehydrogenase, putative / NA... 77 4e-15
At3g09810.1 68416.m01169 isocitrate dehydrogenase, putative / NA... 77 4e-15
At1g32480.1 68414.m04008 isocitrate/isopropylmalate dehydrogenas... 68 4e-12
At1g80560.1 68414.m09445 3-isopropylmalate dehydrogenase, chloro... 33 0.093
At1g31180.1 68414.m03817 3-isopropylmalate dehydrogenase, chloro... 31 0.28
At5g07760.1 68418.m00888 formin homology 2 domain-containing pro... 31 0.49
At5g14200.1 68418.m01659 3-isopropylmalate dehydrogenase, chloro... 30 0.86
At2g27280.1 68415.m03278 hypothetical protein 30 0.86
At2g05420.1 68415.m00570 meprin and TRAF homology domain-contain... 29 1.1
At1g21540.1 68414.m02694 AMP-binding protein, putative strong si... 29 1.5
At2g22890.1 68415.m02717 expressed protein ; expression support... 28 2.6
At5g54860.1 68418.m06834 integral membrane transporter family pr... 28 3.5
At4g18620.1 68417.m02757 hypothetical protein various predicted ... 28 3.5
At5g48920.1 68418.m06052 hydroxyproline-rich glycoprotein family... 27 6.1
At5g19420.1 68418.m02314 zinc finger protein, putative / regulat... 27 6.1
At5g12350.1 68418.m01453 zinc finger protein, putative / regulat... 27 6.1
At3g32190.1 68416.m04102 hypothetical protein 27 8.1
At1g17080.1 68414.m02079 expressed protein 27 8.1
>At4g35260.1 68417.m05011 isocitrate dehydrogenase subunit 1 / NAD+
isocitrate dehydrogenase subunit 1 nearly identical to
NAD+ dependent isocitrate dehydrogenase subunit 1
[Arabidopsis thaliana] GI:1766046
Length = 367
Score = 89.4 bits (212), Expect = 1e-18
Identities = 39/63 (61%), Positives = 53/63 (84%)
Frame = +1
Query: 268 VTSRNVALRNELDMYAYILNCKSYPGVATRHKDIDVVIIRQNTEGEYAMLEHESVNGVVE 447
V+S NV LR ELD++A ++NC + PG+ TRH+++D+V+IR+NTEGEYA LEHE V GVVE
Sbjct: 108 VSSLNVQLRKELDLFASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVVE 167
Query: 448 SMK 456
S+K
Sbjct: 168 SLK 170
Score = 39.5 bits (88), Expect = 0.001
Identities = 19/50 (38%), Positives = 27/50 (54%)
Frame = +3
Query: 24 IPKAQYGGRHAVTMLPGGGIGPECMGYVRDIFKYIGAPIDFEVVDIDPTM 173
+P+ G AVT++PG GIGP V + + + API FE D+ M
Sbjct: 29 MPRPGDGAPRAVTLIPGDGIGPLVTNAVEQVMEAMHAPIFFEKYDVHGEM 78
>At2g17130.2 68415.m01978 isocitrate dehydrogenase subunit 2 / NAD+
isocitrate dehydrogenase subunit 2 nearly identical to
NAD+ dependent isocitrate dehydrogenase subunit 2
[Arabidopsis thaliana] GI:1766048
Length = 363
Score = 89.4 bits (212), Expect = 1e-18
Identities = 39/63 (61%), Positives = 54/63 (85%)
Frame = +1
Query: 268 VTSRNVALRNELDMYAYILNCKSYPGVATRHKDIDVVIIRQNTEGEYAMLEHESVNGVVE 447
V+S NV LR ELD++A ++NC + PG+A+RH+++D+V+IR+NTEGEYA LEHE V GVVE
Sbjct: 108 VSSLNVNLRKELDLFASLVNCFNLPGLASRHENVDIVVIRENTEGEYAGLEHEVVPGVVE 167
Query: 448 SMK 456
S+K
Sbjct: 168 SLK 170
Score = 34.7 bits (76), Expect = 0.030
Identities = 15/50 (30%), Positives = 27/50 (54%)
Frame = +3
Query: 24 IPKAQYGGRHAVTMLPGGGIGPECMGYVRDIFKYIGAPIDFEVVDIDPTM 173
+P+ G VT++PG G+GP V+ + + + AP+ FE ++ M
Sbjct: 29 MPRPGDGKPRPVTLIPGDGVGPLVTNAVQQVMEAMHAPVYFEPFEVHGDM 78
>At2g17130.1 68415.m01977 isocitrate dehydrogenase subunit 2 / NAD+
isocitrate dehydrogenase subunit 2 nearly identical to
NAD+ dependent isocitrate dehydrogenase subunit 2
[Arabidopsis thaliana] GI:1766048
Length = 367
Score = 89.4 bits (212), Expect = 1e-18
Identities = 39/63 (61%), Positives = 54/63 (85%)
Frame = +1
Query: 268 VTSRNVALRNELDMYAYILNCKSYPGVATRHKDIDVVIIRQNTEGEYAMLEHESVNGVVE 447
V+S NV LR ELD++A ++NC + PG+A+RH+++D+V+IR+NTEGEYA LEHE V GVVE
Sbjct: 108 VSSLNVNLRKELDLFASLVNCFNLPGLASRHENVDIVVIRENTEGEYAGLEHEVVPGVVE 167
Query: 448 SMK 456
S+K
Sbjct: 168 SLK 170
Score = 34.7 bits (76), Expect = 0.030
Identities = 15/50 (30%), Positives = 27/50 (54%)
Frame = +3
Query: 24 IPKAQYGGRHAVTMLPGGGIGPECMGYVRDIFKYIGAPIDFEVVDIDPTM 173
+P+ G VT++PG G+GP V+ + + + AP+ FE ++ M
Sbjct: 29 MPRPGDGKPRPVTLIPGDGVGPLVTNAVQQVMEAMHAPVYFEPFEVHGDM 78
>At4g35650.1 68417.m05062 isocitrate dehydrogenase, putative / NAD+
isocitrate dehydrogenase, putative strong similarity to
NAD+ dependent isocitrate dehydrogenase subunit 1
[Arabidopsis thaliana] GI:1766046
Length = 368
Score = 87.0 bits (206), Expect = 5e-18
Identities = 37/63 (58%), Positives = 53/63 (84%)
Frame = +1
Query: 268 VTSRNVALRNELDMYAYILNCKSYPGVATRHKDIDVVIIRQNTEGEYAMLEHESVNGVVE 447
V+S N+ LR ELD++A ++NC + PG+ TRH+++D+V+IR+NTEGEY+ LEHE V GVVE
Sbjct: 109 VSSLNMQLRKELDIFASLVNCINVPGLVTRHENVDIVVIRENTEGEYSGLEHEVVPGVVE 168
Query: 448 SMK 456
S+K
Sbjct: 169 SLK 171
Score = 38.3 bits (85), Expect = 0.002
Identities = 16/46 (34%), Positives = 26/46 (56%)
Frame = +3
Query: 24 IPKAQYGGRHAVTMLPGGGIGPECMGYVRDIFKYIGAPIDFEVVDI 161
+P+ G VT++PG GIGP G V + + + AP+ FE ++
Sbjct: 30 MPRPGDGAPRTVTLIPGDGIGPLVTGAVEQVMEAMHAPVHFERYEV 75
>At5g03290.1 68418.m00279 isocitrate dehydrogenase, putative / NAD+
isocitrate dehydrogenase, putative strong similarity to
isocitrate dehydrogenase (NAD+) [Nicotiana tabacum]
GI:3021506
Length = 374
Score = 77.4 bits (182), Expect = 4e-15
Identities = 34/61 (55%), Positives = 46/61 (75%)
Frame = +1
Query: 274 SRNVALRNELDMYAYILNCKSYPGVATRHKDIDVVIIRQNTEGEYAMLEHESVNGVVESM 453
S N+ LR EL++YA + C S PG TR+ D+D++ IR+NTEGEY+ LEH+ V GVVES+
Sbjct: 121 SLNLTLRKELNLYANVRPCYSLPGYKTRYDDVDLITIRENTEGEYSGLEHQVVRGVVESL 180
Query: 454 K 456
K
Sbjct: 181 K 181
Score = 34.7 bits (76), Expect = 0.030
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = +3
Query: 60 TMLPGGGIGPECMGYVRDIFKYIGAPIDFE 149
T+ PG GIGPE V+ +F G PI++E
Sbjct: 47 TLFPGDGIGPEIAESVKKVFTTAGVPIEWE 76
>At3g09810.1 68416.m01169 isocitrate dehydrogenase, putative / NAD+
isocitrate dehydrogenase, putative strong similarity to
isocitrate dehydrogenase (NAD+) GB:CAA65502 GI:3021506
[Nicotiana tabacum]
Length = 374
Score = 77.4 bits (182), Expect = 4e-15
Identities = 34/61 (55%), Positives = 46/61 (75%)
Frame = +1
Query: 274 SRNVALRNELDMYAYILNCKSYPGVATRHKDIDVVIIRQNTEGEYAMLEHESVNGVVESM 453
S N+ LR EL++YA + C S PG TR+ D+D++ IR+NTEGEY+ LEH+ V GVVES+
Sbjct: 121 SLNLTLRKELNLYANVRPCYSLPGYKTRYDDVDLITIRENTEGEYSGLEHQVVKGVVESL 180
Query: 454 K 456
K
Sbjct: 181 K 181
Score = 29.1 bits (62), Expect = 1.5
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = +3
Query: 60 TMLPGGGIGPECMGYVRDIFKYIGAPIDFE 149
T+ PG GIGPE V+ +F ID++
Sbjct: 47 TLFPGDGIGPEIAESVKQVFTAADVVIDWD 76
>At1g32480.1 68414.m04008 isocitrate/isopropylmalate dehydrogenase
family protein similar to NAD+ dependent isocitrate
dehydrogenase subunit 2 [Arabidopsis thaliana]
GI:1766048; contains Pfam profile PF00180 dehydrogenase,
isocitrate/isopropylmalate family
Length = 294
Score = 67.7 bits (158), Expect = 4e-12
Identities = 28/55 (50%), Positives = 43/55 (78%)
Frame = +1
Query: 292 RNELDMYAYILNCKSYPGVATRHKDIDVVIIRQNTEGEYAMLEHESVNGVVESMK 456
R ELD++A +++C + G +RH+++D+V+IR+NTEGEYA EHE V GV+ES +
Sbjct: 70 RKELDLFASLVDCFNLNGQPSRHENVDIVVIRENTEGEYAGREHEVVPGVIESFQ 124
>At1g80560.1 68414.m09445 3-isopropylmalate dehydrogenase,
chloroplast, putative strong similarity to
3-ISOPROPYLMALATE DEHYDROGENASE PRECURSOR GB:P29102
SP|P29102 from [Brassica napus]
Length = 405
Score = 33.1 bits (72), Expect = 0.093
Identities = 11/29 (37%), Positives = 20/29 (68%)
Frame = +3
Query: 48 RHAVTMLPGGGIGPECMGYVRDIFKYIGA 134
R+ +T+LPG GIGPE + +++ + G+
Sbjct: 42 RYTITLLPGDGIGPEVVSIAKNVLQQAGS 70
>At1g31180.1 68414.m03817 3-isopropylmalate dehydrogenase,
chloroplast, putative strong similarity to SP|P29102
3-isopropylmalate dehydrogenase, chloroplast precursor
{Brassica napus}; EST gb|F14478 comes from this gene
Length = 404
Score = 31.5 bits (68), Expect = 0.28
Identities = 11/28 (39%), Positives = 19/28 (67%)
Frame = +3
Query: 48 RHAVTMLPGGGIGPECMGYVRDIFKYIG 131
R+ +T+LPG GIGPE + +++ + G
Sbjct: 43 RYNITLLPGDGIGPEVISVAKNVLQKAG 70
>At5g07760.1 68418.m00888 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 853
Score = 30.7 bits (66), Expect = 0.49
Identities = 14/27 (51%), Positives = 16/27 (59%), Gaps = 1/27 (3%)
Frame = -2
Query: 85 PIPPPGSMVTA*RPPYCAL-GISFCFP 8
P+PPP + PP CAL GI CFP
Sbjct: 99 PLPPPPLIFVGAPPPTCALKGIVCCFP 125
>At5g14200.1 68418.m01659 3-isopropylmalate dehydrogenase,
chloroplast, putative strong similarity to SP|P29102
3-isopropylmalate dehydrogenase, chloroplast precursor
{Brassica napus}
Length = 409
Score = 29.9 bits (64), Expect = 0.86
Identities = 10/29 (34%), Positives = 19/29 (65%)
Frame = +3
Query: 48 RHAVTMLPGGGIGPECMGYVRDIFKYIGA 134
R+ + +LPG GIGPE + +++ + G+
Sbjct: 46 RYNIALLPGDGIGPEVISVAKNVLQKAGS 74
>At2g27280.1 68415.m03278 hypothetical protein
Length = 427
Score = 29.9 bits (64), Expect = 0.86
Identities = 15/34 (44%), Positives = 21/34 (61%)
Frame = -1
Query: 128 DVFEYVANVSHALRTDTASREHGHRVTTAVLRLR 27
+V +++ S+ LR DTA +E VTTA RLR
Sbjct: 26 NVLDFIGKESNFLRKDTAEKEITDAVTTAKERLR 59
>At2g05420.1 68415.m00570 meprin and TRAF homology domain-containing
protein / MATH domain-containing protein similar to
ubiquitin-specific protease 12 [Arabidopsis thaliana]
GI:11993471; contains Pfam profile PF00917: MATH domain
Length = 297
Score = 29.5 bits (63), Expect = 1.1
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = +1
Query: 295 NELDMYAYILNCKSYPGVATRHKDIDVVIIRQNTE 399
N L +Y + N KS+P TRH + ++ Q +E
Sbjct: 57 NNLALYLNVANSKSFPIGWTRHTKFSLTLVNQKSE 91
>At1g21540.1 68414.m02694 AMP-binding protein, putative strong
similarity to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501; identical to cDNA adenosine monophosphate
binding protein 9 AMPBP9 (AMPBP9) GI:20799726
Length = 550
Score = 29.1 bits (62), Expect = 1.5
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = -2
Query: 133 APMYLNMSRTYPMHSGPIPPPGSMVTA*RPPYCAL 29
APM LNM YP P+ P ++TA PP A+
Sbjct: 282 APMVLNMLTNYPSRK-PLKNPVQVMTAGAPPPAAI 315
>At2g22890.1 68415.m02717 expressed protein ; expression supported
by MPSS
Length = 279
Score = 28.3 bits (60), Expect = 2.6
Identities = 12/19 (63%), Positives = 14/19 (73%)
Frame = -3
Query: 444 DHTIHGFVFQHSVFTFCVL 388
DH +HGFV S+F FCVL
Sbjct: 176 DHVVHGFV---SMFAFCVL 191
>At5g54860.1 68418.m06834 integral membrane transporter family
protein contains 10 transmembrane domains; contains Pfam
PF03092: BT1 family; contains TIGRFAMS TIGR00788:
folate/biopterin transporter; similar to high affinity
folic acid/methotrexate transporter 5 (GI:21898554)
[Leishmania tarentolae]
Length = 491
Score = 27.9 bits (59), Expect = 3.5
Identities = 11/35 (31%), Positives = 20/35 (57%)
Frame = +3
Query: 240 TLKPKXGSLCXVTQCGSQKRTGHVCLHTEL*ILPW 344
++KP G + G +KRT ++ + T L ++PW
Sbjct: 64 SIKPLYGIISDCIPIGGKKRTPYLVISTVLSLVPW 98
>At4g18620.1 68417.m02757 hypothetical protein various predicted
proteins, Arabidopsis thaliana
Length = 164
Score = 27.9 bits (59), Expect = 3.5
Identities = 15/40 (37%), Positives = 21/40 (52%)
Frame = +3
Query: 72 GGGIGPECMGYVRDIFKYIGAPIDFEVVDIDPTMDNDDDV 191
GGG G E G VRD+ G P DF ++ +D++ V
Sbjct: 51 GGGKGGEGKGSVRDVTLVSGFPADFSTERLE-ELDDESHV 89
>At5g48920.1 68418.m06052 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 205
Score = 27.1 bits (57), Expect = 6.1
Identities = 12/31 (38%), Positives = 18/31 (58%)
Frame = -2
Query: 130 PMYLNMSRTYPMHSGPIPPPGSMVTA*RPPY 38
P Y S +P+ S P+PPP S ++ PP+
Sbjct: 9 PYYSPPSHQHPLPS-PVPPPPSHISPPPPPF 38
>At5g19420.1 68418.m02314 zinc finger protein, putative / regulator
of chromosome condensation (RCC1) family protein similar
to zinc finger protein [Arabidopsis thaliana]
gi|15811367|gb|AAL08940
Length = 1124
Score = 27.1 bits (57), Expect = 6.1
Identities = 13/30 (43%), Positives = 15/30 (50%)
Frame = -2
Query: 154 TTSKSIGAPMYLNMSRTYPMHSGPIPPPGS 65
T S+S G P N RTY S P+ P S
Sbjct: 158 TESRSDGTPSEANSPRTYTRRSSPLHSPFS 187
>At5g12350.1 68418.m01453 zinc finger protein, putative / regulator
of chromosome condensation (RCC1) family protein similar
to zinc finger protein [Arabidopsis thaliana]
gi|15811367|gb|AAL08940
Length = 1062
Score = 27.1 bits (57), Expect = 6.1
Identities = 13/30 (43%), Positives = 15/30 (50%)
Frame = -2
Query: 154 TTSKSIGAPMYLNMSRTYPMHSGPIPPPGS 65
T S+S G P N RTY S P+ P S
Sbjct: 125 TESRSDGTPSEANSPRTYTRRSSPLHSPFS 154
>At3g32190.1 68416.m04102 hypothetical protein
Length = 358
Score = 26.6 bits (56), Expect = 8.1
Identities = 14/30 (46%), Positives = 19/30 (63%), Gaps = 1/30 (3%)
Frame = -3
Query: 405 FTFCVLSDNNNINVLMSGR-NARVRFTVQY 319
F F VLSDN++IN S R N+ + +QY
Sbjct: 32 FKFQVLSDNSHINAHHSSRTNSELNDMIQY 61
>At1g17080.1 68414.m02079 expressed protein
Length = 154
Score = 26.6 bits (56), Expect = 8.1
Identities = 14/40 (35%), Positives = 20/40 (50%)
Frame = -2
Query: 136 GAPMYLNMSRTYPMHSGPIPPPGSMVTA*RPPYCALGISF 17
GAP Y + +T P+H G G V R P C +G+ +
Sbjct: 57 GAPYYAHGYQTVPVHGG--VAEGRPVRQRRLPCCGIGLGW 94
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,525,363
Number of Sequences: 28952
Number of extensions: 214282
Number of successful extensions: 583
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 562
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 581
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 762235320
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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