Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00368
(738 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 127 2e-30
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 126 4e-30
SPAC17H9.06c |||conserved fungal protein|Schizosaccharomyces pom... 29 0.91
SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pomb... 27 2.1
SPAC57A10.05c |pof1||F-box protein Pof1|Schizosaccharomyces pomb... 27 2.1
SPAC5H10.09c |||3-methyl-2-oxobutanoatehydroxymethyltransferase|... 27 2.8
SPBC16H5.05c |cyp7|cwf27|cyclophilin family peptidyl-prolyl cis-... 27 3.7
SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pom... 27 3.7
SPBC3E7.11c |||DNAJ protein Caj1/Djp1-type|Schizosaccharomyces p... 27 3.7
SPBC839.10 |usp107|snu71|U1 snRNP-associated protein Usp107|Schi... 26 4.9
SPAC1420.01c ||SPAC56E4.08c|DUF1752 family protein|Schizosacchar... 25 8.5
SPBC6B1.04 |mde4||monopolin-like complex subunit Mde4|Schizosacc... 25 8.5
>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 127 bits (306), Expect = 2e-30
Identities = 73/149 (48%), Positives = 95/149 (63%), Gaps = 4/149 (2%)
Frame = +3
Query: 255 KNVVGARRSSWRVISSIEQKTE--GSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLI 428
KNV+GARR+SWR++SSIEQK E G+ + ++ KEYR K+E+EL IC D+L +L+KHLI
Sbjct: 52 KNVIGARRASWRIVSSIEQKEESKGNTAQVELIKEYRQKIEQELDTICQDILTVLEKHLI 111
Query: 429 PKASNPESKVFYLKMKGDYYRYLAEVAQEKPDILL*RIHRKHTKMLLKSARRKCSPHTP- 605
P A++ ESKVFY KMKGDYYRYLAE A + + K + A + +P P
Sbjct: 112 PNAASAESKVFYYKMKGDYYRYLAEFAVGEKRQHSADQSLEGYKAASEIATAELAPTHPI 171
Query: 606 -*GWVWR*ISQSLL*VLNSPDKACQLAKQ 689
G S +LNSPD+AC LAKQ
Sbjct: 172 RLGLALN-FSVFYYEILNSPDRACYLAKQ 199
Score = 62.5 bits (145), Expect = 6e-11
Identities = 33/71 (46%), Positives = 44/71 (61%), Gaps = 1/71 (1%)
Frame = +2
Query: 512 GETRHSVVEDSQKAYQDAFEISKAKMQPTHPIRLGLALNFSVFIMSI-KFARQGVSTRQT 688
GE R + S + Y+ A EI+ A++ PTHPIRLGLALNFSVF I + +
Sbjct: 140 GEKRQHSADQSLEGYKAASEIATAELAPTHPIRLGLALNFSVFYYEILNSPDRACYLAKQ 199
Query: 689 GFDDAIAELDT 721
FD+AI+ELD+
Sbjct: 200 AFDEAISELDS 210
Score = 54.4 bits (125), Expect = 2e-08
Identities = 28/48 (58%), Positives = 33/48 (68%)
Frame = +1
Query: 109 SVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVA 252
+ +E+ V AKLAEQAERY+ M MK V T EL+ EERNLLSVA
Sbjct: 3 TTSREDAVYLAKLAEQAERYEGMVENMKSVASTDQELTVEERNLLSVA 50
>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 126 bits (303), Expect = 4e-30
Identities = 77/155 (49%), Positives = 96/155 (61%), Gaps = 9/155 (5%)
Frame = +3
Query: 255 KNVVGARRSSWRVISSIEQKTE--GSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLI 428
KN++GARR+SWR+ISSIEQK E G+ R+ + KEYR K+E EL +IC+DVL +L+KHLI
Sbjct: 51 KNIIGARRASWRIISSIEQKEESRGNTRQAALIKEYRKKIEDELSDICHDVLSVLEKHLI 110
Query: 429 PKASNPESKVFYLKMKGDYYRYLA-----EVAQEKPDILL*RIHRKHTKMLLKSARRKCS 593
P A+ ESKVFY KMKGDYYRYLA EV +E D L + K A +
Sbjct: 111 PAATTGESKVFYYKMKGDYYRYLAEFTVGEVCKEAADSSL-----EAYKAASDIAVAELP 165
Query: 594 PHTP--*GWVWR*ISQSLL*VLNSPDKACQLAKQV 692
P P G S +L+SP+ AC LAKQV
Sbjct: 166 PTDPMRLGLALN-FSVFYYEILDSPESACHLAKQV 199
Score = 55.2 bits (127), Expect = 9e-09
Identities = 30/71 (42%), Positives = 44/71 (61%), Gaps = 1/71 (1%)
Frame = +2
Query: 512 GETRHSVVEDSQKAYQDAFEISKAKMQPTHPIRLGLALNFSVFIMSIKFARQGV-STRQT 688
GE + S +AY+ A +I+ A++ PT P+RLGLALNFSVF I + + +
Sbjct: 139 GEVCKEAADSSLEAYKAASDIAVAELPPTDPMRLGLALNFSVFYYEILDSPESACHLAKQ 198
Query: 689 GFDDAIAELDT 721
FD+AI+ELD+
Sbjct: 199 VFDEAISELDS 209
Score = 53.2 bits (122), Expect = 4e-08
Identities = 29/49 (59%), Positives = 35/49 (71%)
Frame = +1
Query: 106 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVA 252
MS +E V AKLAEQAERY++M MK+V + +LS EERNLLSVA
Sbjct: 1 MSNSRENSVYLAKLAEQAERYEEMVENMKKVACSNDKLSVEERNLLSVA 49
>SPAC17H9.06c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 601
Score = 28.7 bits (61), Expect = 0.91
Identities = 26/91 (28%), Positives = 41/91 (45%), Gaps = 9/91 (9%)
Frame = +3
Query: 288 RVISSIEQKTEGSERKQQMAKEYRVKVEKE-LREICYDVLGL--LDKHL---IPKASNPE 449
+V+SS + E S + + R E E L E+ +V L +D+ +P+ NP
Sbjct: 263 KVLSSSSEDDESSSPEDLLKPIIRSTEEMENLNELEQEVQDLDPIDEGFEEKVPRFRNPS 322
Query: 450 SKVFYLK---MKGDYYRYLAEVAQEKPDILL 533
K FY K ++ Y L + EK D L+
Sbjct: 323 KKAFYEKLHSLRNRSYSKLESLTSEKSDTLI 353
>SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1207
Score = 27.5 bits (58), Expect = 2.1
Identities = 17/50 (34%), Positives = 28/50 (56%), Gaps = 1/50 (2%)
Frame = +2
Query: 515 ETRHSVVEDSQKAYQDAFEISKAKMQPTHP-IRLGLALNFSVFIMSIKFA 661
E R ED+++A + ++ + A +QP H I L LA+N S + + K A
Sbjct: 1059 ERRRLGEEDTKRALELSYYFASADLQPMHSIIALRLAINASHKLKNYKSA 1108
>SPAC57A10.05c |pof1||F-box protein Pof1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 605
Score = 27.5 bits (58), Expect = 2.1
Identities = 15/38 (39%), Positives = 22/38 (57%)
Frame = -1
Query: 567 KASWYAFCESSTTECLVSPVPLLQGTCSNLLSF*GKTL 454
+A W AF E+S +E ++ +L S+LLSF TL
Sbjct: 64 EAVWAAFSEASCSERKLALQGILNNCSSSLLSFASSTL 101
>SPAC5H10.09c
|||3-methyl-2-
oxobutanoatehydroxymethyltransferase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 267
Score = 27.1 bits (57), Expect = 2.8
Identities = 13/35 (37%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +1
Query: 475 KEITTGTLQKWHRRNQTFCC-RGFTESIPRCF*NQ 576
K+IT TL++W N+ F C + S R F Q
Sbjct: 4 KQITISTLRQWKLANKKFACITAYDASFSRLFAEQ 38
>SPBC16H5.05c |cyp7|cwf27|cyclophilin family peptidyl-prolyl
cis-trans isomerase Cyp7|Schizosaccharomyces pombe|chr
2|||Manual
Length = 463
Score = 26.6 bits (56), Expect = 3.7
Identities = 12/39 (30%), Positives = 20/39 (51%)
Frame = +3
Query: 255 KNVVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVE 371
KN+ S+ R +SS + K +E + M +Y K+E
Sbjct: 350 KNLENDEESTLRALSSFQSKIRNAEDEDVMDSQYGSKIE 388
>SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1067
Score = 26.6 bits (56), Expect = 3.7
Identities = 17/48 (35%), Positives = 22/48 (45%)
Frame = +2
Query: 5 TREKLILCLNLLVQIHFSPSDKGISELVLFHRPRCPSTRKNWCNVPNW 148
T EKL+ C HF +G+ + L RC +T CNVP W
Sbjct: 681 TVEKLVACTT-----HFY---EGVRKSALSSLWRCATTYYKVCNVPQW 720
>SPBC3E7.11c |||DNAJ protein Caj1/Djp1-type|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 355
Score = 26.6 bits (56), Expect = 3.7
Identities = 21/69 (30%), Positives = 37/69 (53%), Gaps = 2/69 (2%)
Frame = +3
Query: 306 EQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKM-KGD 482
E E+ Q++A+ Y+V + +LRE YD LG + +P A ++ F+ + GD
Sbjct: 43 ENPEAAREKFQKLAEAYQVLSDPKLRE-KYDKLGKVG--AVPDAGFEDAFEFFKNLFGGD 99
Query: 483 YYR-YLAEV 506
+R Y+ E+
Sbjct: 100 SFRDYVGEL 108
>SPBC839.10 |usp107|snu71|U1 snRNP-associated protein
Usp107|Schizosaccharomyces pombe|chr 2|||Manual
Length = 695
Score = 26.2 bits (55), Expect = 4.9
Identities = 13/42 (30%), Positives = 22/42 (52%)
Frame = +3
Query: 309 QKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPK 434
+K + + KEY K +++LR+ D+ LL KH I +
Sbjct: 328 EKQRSKDEYASLYKEYTRKEQEKLRKQNDDLQNLLSKHRISR 369
>SPAC1420.01c ||SPAC56E4.08c|DUF1752 family
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 580
Score = 25.4 bits (53), Expect = 8.5
Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 1/42 (2%)
Frame = +2
Query: 53 FSPSDK-GISELVLFHRPRCPSTRKNWCNVPNWPNKLSDMTT 175
FSP +K + +L LFH + PS+++ V N + SD +T
Sbjct: 162 FSPPEKPSMKDLALFHGNKSPSSKETIPKVSN--SNSSDTST 201
>SPBC6B1.04 |mde4||monopolin-like complex subunit
Mde4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 421
Score = 25.4 bits (53), Expect = 8.5
Identities = 16/43 (37%), Positives = 25/43 (58%), Gaps = 2/43 (4%)
Frame = +3
Query: 327 ERKQQMAKEYRVKVEKELREICYDV--LGLLDKHLIPKASNPE 449
E++Q A +YR+KVE+ +I V + L+ L + SNPE
Sbjct: 85 EQEQNEANDYRLKVERLEHKISDYVQEINSLNSQLQIQKSNPE 127
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,116,878
Number of Sequences: 5004
Number of extensions: 63511
Number of successful extensions: 205
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 187
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 201
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 349251756
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -