Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00368
(738 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_34219| Best HMM Match : No HMM Matches (HMM E-Value=.) 124 6e-29
SB_34217| Best HMM Match : No HMM Matches (HMM E-Value=.) 105 4e-23
SB_36368| Best HMM Match : 14-3-3 (HMM E-Value=0) 94 1e-19
SB_37955| Best HMM Match : 14-3-3 (HMM E-Value=6.5861e-44) 80 2e-15
SB_34218| Best HMM Match : No HMM Matches (HMM E-Value=.) 50 2e-06
SB_42896| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 1.7
SB_15350| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 2.3
SB_45140| Best HMM Match : RVT_1 (HMM E-Value=3.8e-32) 29 5.2
SB_11347| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 5.2
SB_41987| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 6.9
SB_39229| Best HMM Match : Phage_holin_1 (HMM E-Value=3.5) 28 6.9
SB_12646| Best HMM Match : SSrecog (HMM E-Value=0) 28 6.9
>SB_34219| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 309
Score = 124 bits (300), Expect = 6e-29
Identities = 60/85 (70%), Positives = 67/85 (78%)
Frame = +3
Query: 255 KNVVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPK 434
KNVVGA+RSSWRVISSIEQK EGSERK+Q + YR +E EL E+C VL LL+ LIP
Sbjct: 28 KNVVGAKRSSWRVISSIEQKLEGSERKKQNTETYRQTIENELNEVCETVLKLLESKLIPN 87
Query: 435 ASNPESKVFYLKMKGDYYRYLAEVA 509
A + ESKVFYLKMKGDYYRY EVA
Sbjct: 88 AQSTESKVFYLKMKGDYYRYEGEVA 112
Score = 67.3 bits (157), Expect = 1e-11
Identities = 37/72 (51%), Positives = 48/72 (66%), Gaps = 3/72 (4%)
Frame = +2
Query: 515 ETRHSVVEDSQKAYQDAFEISKA--KMQPTHPIRLGLALNFSVFIMSI-KFARQGVSTRQ 685
+ R VV+ + KAY +A EI++ K+ PT PIRLGLALNFSVF I + ++Q +
Sbjct: 115 DRRREVVQKAMKAYSEAQEIAEKDPKLPPTDPIRLGLALNFSVFYYEIVEDSKQACDLAK 174
Query: 686 TGFDDAIAELDT 721
FDDAIAELDT
Sbjct: 175 KAFDDAIAELDT 186
Score = 56.8 bits (131), Expect = 2e-08
Identities = 33/69 (47%), Positives = 45/69 (65%), Gaps = 4/69 (5%)
Frame = +2
Query: 527 SVVEDSQKAYQDAFEISKA---KMQPTHPIRLGLALNFSVFIMSI-KFARQGVSTRQTGF 694
+VVE + +AY++A E ++ K+ PT PIRLGLALNFSVF I + + + F
Sbjct: 207 TVVEKALQAYKEAKEAAETGDGKLAPTDPIRLGLALNFSVFHYEIQENQEEACKLAKQAF 266
Query: 695 DDAIAELDT 721
DDAIAELD+
Sbjct: 267 DDAIAELDS 275
Score = 35.5 bits (78), Expect = 0.045
Identities = 18/26 (69%), Positives = 18/26 (69%)
Frame = +1
Query: 175 MAAAMKEVTETGVELSNEERNLLSVA 252
MA AMKE TE L EERNLLSVA
Sbjct: 1 MAKAMKEATEISETLEQEERNLLSVA 26
>SB_34217| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 219
Score = 105 bits (252), Expect = 4e-23
Identities = 50/83 (60%), Positives = 66/83 (79%)
Frame = +3
Query: 255 KNVVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPK 434
KN+VG RRSSWRVISSIE+KT S + K+Y+ +EKEL+++C +VLG+L++ LIP
Sbjct: 130 KNIVGQRRSSWRVISSIEEKTAESS-SLAIVKKYKACIEKELKDLCKEVLGILER-LIPG 187
Query: 435 ASNPESKVFYLKMKGDYYRYLAE 503
A + E+KVFY K+KGDYYRYLAE
Sbjct: 188 AEDEENKVFYFKLKGDYYRYLAE 210
Score = 58.8 bits (136), Expect = 4e-09
Identities = 29/49 (59%), Positives = 37/49 (75%)
Frame = +1
Query: 106 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVA 252
M V +E L+ AKL+EQ +RYD+MA MKEV+E +LS EERNLLSV+
Sbjct: 80 MMVVRETLIYNAKLSEQCDRYDEMAKIMKEVSEKYPKLSKEERNLLSVS 128
>SB_36368| Best HMM Match : 14-3-3 (HMM E-Value=0)
Length = 248
Score = 93.9 bits (223), Expect = 1e-19
Identities = 64/151 (42%), Positives = 84/151 (55%), Gaps = 6/151 (3%)
Frame = +3
Query: 255 KNVVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPK 434
KNVVGARRSSWRVISS+EQK E + K+YR + EL C +VL +L+ +L+
Sbjct: 57 KNVVGARRSSWRVISSMEQKAP--EEMAALTKKYREDITNELNGKCAEVLDILENYLLKD 114
Query: 435 AS---NPESKVFYLKMKGDYYRYLAEVAQEKPDILL*RIHRKHTKMLLKSAR-RKCSPHT 602
N E+KVFYLKM+GDY+RYL EVA+ D I + SA+ + SP
Sbjct: 115 GQDDINTEAKVFYLKMRGDYHRYLVEVAE--GDSRKENIEKSREAYKDASAKAEELSPSH 172
Query: 603 P--*GWVWR*ISQSLL*VLNSPDKACQLAKQ 689
P G S + N P +AC+LAK+
Sbjct: 173 PIRLGLALN-FSVFYYEIENKPPEACKLAKE 202
Score = 64.1 bits (149), Expect = 1e-10
Identities = 31/53 (58%), Positives = 38/53 (71%)
Frame = +1
Query: 94 PSSTMSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVA 252
P+ +EEL+ AK+AEQAERYDDM AM VT+ G L++EERNLLSVA
Sbjct: 3 PNFVSKCSREELIHLAKMAEQAERYDDMVNAMSAVTKEGKPLNDEERNLLSVA 55
Score = 59.3 bits (137), Expect = 3e-09
Identities = 32/70 (45%), Positives = 44/70 (62%), Gaps = 1/70 (1%)
Frame = +2
Query: 512 GETRHSVVEDSQKAYQDAFEISKAKMQPTHPIRLGLALNFSVFIMSIK-FARQGVSTRQT 688
G++R +E S++AY+DA ++ P+HPIRLGLALNFSVF I+ + +
Sbjct: 144 GDSRKENIEKSREAYKDA-SAKAEELSPSHPIRLGLALNFSVFYYEIENKPPEACKLAKE 202
Query: 689 GFDDAIAELD 718
FDDAIA LD
Sbjct: 203 AFDDAIAVLD 212
>SB_37955| Best HMM Match : 14-3-3 (HMM E-Value=6.5861e-44)
Length = 251
Score = 80.2 bits (189), Expect = 2e-15
Identities = 39/76 (51%), Positives = 53/76 (69%), Gaps = 3/76 (3%)
Frame = +3
Query: 255 KNVVGARRSSWRVISSIEQKTEGSER---KQQMAKEYRVKVEKELREICYDVLGLLDKHL 425
KNV+GARR+SWR+I+SIEQK E K +M + YR +E+EL+ IC ++L LLD L
Sbjct: 50 KNVIGARRASWRIITSIEQKEESKGEDMAKLEMIRNYRKTIEEELKTICGEILSLLDDSL 109
Query: 426 IPKASNPESKVFYLKM 473
I + + ESKVFY K+
Sbjct: 110 IKNSQSEESKVFYNKI 125
Score = 68.5 bits (160), Expect = 5e-12
Identities = 34/46 (73%), Positives = 37/46 (80%)
Frame = +1
Query: 115 DKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVA 252
DKEE V AKLAEQAERYDDM +MKEV + G ELS E+RNLLSVA
Sbjct: 3 DKEEHVYMAKLAEQAERYDDMVNSMKEVAKMGTELSTEDRNLLSVA 48
>SB_34218| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 607
Score = 50.0 bits (114), Expect = 2e-06
Identities = 25/48 (52%), Positives = 31/48 (64%)
Frame = +1
Query: 106 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSV 249
M + ELVQ AKLAEQ ER++D+ MK+ E L+ E RNLLSV
Sbjct: 186 MQDSRNELVQLAKLAEQTERFEDVILYMKKAIEINPSLNKEHRNLLSV 233
Score = 34.3 bits (75), Expect = 0.10
Identities = 17/52 (32%), Positives = 31/52 (59%), Gaps = 2/52 (3%)
Frame = +3
Query: 255 KNVVGARRSSWRVISSIEQKTEGSERKQQMAK--EYRVKVEKELREICYDVL 404
KNVVG++R +WR + ++ R Q+ +Y+ K+E EL+ +C ++L
Sbjct: 236 KNVVGSKRFAWRHLHHDALQSGRYIRDSQLKGIIKYKEKIEMELKTLCREIL 287
>SB_42896| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 994
Score = 30.3 bits (65), Expect = 1.7
Identities = 16/51 (31%), Positives = 28/51 (54%)
Frame = +1
Query: 82 ISPLPSSTMSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEER 234
+SPLPS+ +E+V KLAE+ E ++++ T+ E + EE+
Sbjct: 643 VSPLPSTATEDQMQEVVDSNKLAEKKEVTEEVSPVKPLSTKESKENALEEK 693
>SB_15350| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1126
Score = 29.9 bits (64), Expect = 2.3
Identities = 27/105 (25%), Positives = 47/105 (44%), Gaps = 2/105 (1%)
Frame = +3
Query: 288 RVISSIEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYL 467
R++ IE+K E E ++ AKE + + E+E + IC L+ + + E + L
Sbjct: 919 RIMKEIEEK-EKKEEAERKAKEEKEREERERKRIC---------QLLKEKAEQERREREL 968
Query: 468 KMKGDYYRYLAE--VAQEKPDILL*RIHRKHTKMLLKSARRKCSP 596
K + + R E + +E+ L + RK + LK K P
Sbjct: 969 KAREEKLRLAREQMMREERERTLKLELERKRMEERLKDQASKNDP 1013
>SB_45140| Best HMM Match : RVT_1 (HMM E-Value=3.8e-32)
Length = 868
Score = 28.7 bits (61), Expect = 5.2
Identities = 12/42 (28%), Positives = 23/42 (54%), Gaps = 2/42 (4%)
Frame = -2
Query: 560 LGMLSVNPLQQNVWFLLCHFCKVPVVI--SFHFEVKHFTFWI 441
L +LS +P++ W+L CK ++ +FE+KH + +
Sbjct: 781 LKLLSEDPIEVQYWYLEWTICKTRNIMLRDLNFEIKHIDYML 822
>SB_11347| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1234
Score = 28.7 bits (61), Expect = 5.2
Identities = 16/56 (28%), Positives = 30/56 (53%)
Frame = +3
Query: 276 RSSWRVISSIEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASN 443
+ W ++S E+ +ER++Q E +VK E++ R+ ++ + HL K SN
Sbjct: 338 KHQWNLLSEDEKNKIKAERRKQKRLELKVKREEKERKRLEELKRVSFAHLGMKLSN 393
>SB_41987| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 125
Score = 28.3 bits (60), Expect = 6.9
Identities = 18/75 (24%), Positives = 42/75 (56%), Gaps = 5/75 (6%)
Frame = +3
Query: 312 KTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDK--HLIPKASNPESKVFYLKMKGDY 485
KT+G + + ++ +++ KE R + + L LDK + + + P+++++ L +Y
Sbjct: 18 KTKGKKALRSISDNETLELAKEFRALFQERLRGLDKKSYHTSEQNKPDTELYVLACFAEY 77
Query: 486 Y--RY-LAEVAQEKP 521
+ +Y + + +QEKP
Sbjct: 78 FPSKYRVTKCSQEKP 92
>SB_39229| Best HMM Match : Phage_holin_1 (HMM E-Value=3.5)
Length = 636
Score = 28.3 bits (60), Expect = 6.9
Identities = 18/44 (40%), Positives = 24/44 (54%), Gaps = 1/44 (2%)
Frame = +2
Query: 455 SVLPQNERRLLQVPCRSGTGE-TRHSVVEDSQKAYQDAFEISKA 583
S LP ERR +P RSGT T+ VV+D+ A I++A
Sbjct: 144 SSLPVEERRASTLPSRSGTPRLTKIDVVDDNAALRAFARRITRA 187
>SB_12646| Best HMM Match : SSrecog (HMM E-Value=0)
Length = 783
Score = 28.3 bits (60), Expect = 6.9
Identities = 11/34 (32%), Positives = 22/34 (64%)
Frame = +3
Query: 333 KQQMAKEYRVKVEKELREICYDVLGLLDKHLIPK 434
K++M ++Y K+EKE+ Y+++ L K ++ K
Sbjct: 292 KEEMKEKYDGKIEKEMSGAIYEIISRLMKAVVGK 325
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 23,599,458
Number of Sequences: 59808
Number of extensions: 492675
Number of successful extensions: 1363
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1250
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1356
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1986074805
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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