Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00358
(740 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr... 173 2e-44
SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 3|... 105 9e-24
SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces pombe... 35 0.011
SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|ch... 33 0.043
SPCC1322.14c |vtc4||vacuolar transporter chaperone |Schizosaccha... 29 0.92
SPAC6F12.12 |par2|pbp2|protein phosphatase regulatory subunit Pa... 27 2.1
SPCPB1C11.02 |||amino acid permease, unknown 16|Schizosaccharomy... 27 2.1
SPAP8A3.14c |||mitochondrial inner membrane protein |Schizosacch... 26 4.9
SPBC16D10.05 |mok13||alpha-1,3-glucan synthase Mok13|Schizosacch... 26 4.9
SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase |Schizosa... 26 4.9
SPBC3B8.10c |||NLI interacting factor family|Schizosaccharomyces... 26 6.5
SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces pombe... 26 6.5
SPCC24B10.22 ||SPCPB16A4.01|mitochondrial DNA polymerase gamma c... 25 8.6
SPBC1861.03 |mak10||NatC N-acetyltransferase complex subunit Mak... 25 8.6
>SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 591
Score = 173 bits (421), Expect = 2e-44
Identities = 83/114 (72%), Positives = 91/114 (79%), Gaps = 1/114 (0%)
Frame = +2
Query: 248 IGYPQIMSPLSKYHRDVPGLTERFEVYVAKKEICNAYTELNDPATQRERFEEQAKNRAAG 427
IG+PQ+MSPL+KYHR GL ERFE +VA KEICNAYTELND QR RFEEQA+ +A G
Sbjct: 467 IGHPQMMSPLAKYHRSDAGLCERFEAFVATKEICNAYTELNDIFDQRARFEEQARQKAQG 526
Query: 428 DDETPPTDEAFCTALEYGLPPTGG-GARVDRLTMFLTDSNNIKEVLLFPAMKPD 586
DDE DE FCTALEYGLPPTGG G VDRL MFLTDSN I+EVLLFP MKP+
Sbjct: 527 DDEAQIIDENFCTALEYGLPPTGGWGMGVDRLVMFLTDSNTIREVLLFPHMKPE 580
Score = 105 bits (251), Expect = 9e-24
Identities = 46/80 (57%), Positives = 59/80 (73%)
Frame = +3
Query: 12 EIDFTPPFARVPMMATLERVLNVKLPAPDKLDTPEANDFLNNLCNTHQIECSPPRTTARL 191
E+DF+ P+ R+ M+ LE LN K P D+L TPEAN FL +LC H +EC+PP+T +RL
Sbjct: 388 ELDFSRPWRRINMIEYLEEKLNTKFPPGDQLHTPEANAFLRDLCAKHGVECAPPQTCSRL 447
Query: 192 LDKLVSVFLEEECINPTFIL 251
LDKLV F+E ECINPTFI+
Sbjct: 448 LDKLVGEFIESECINPTFII 467
>SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 531
Score = 105 bits (251), Expect = 9e-24
Identities = 56/118 (47%), Positives = 72/118 (61%), Gaps = 7/118 (5%)
Frame = +2
Query: 248 IGYPQIMSPLSKYHRDVPGLTE-----RFEVYVAKKEICNAYTELNDPATQRERFEEQAK 412
I +P++MSPL+K + G E RFE+Y+ EICNAY E NDP Q +F+ Q
Sbjct: 412 INHPEVMSPLAKSDIKLYGAVEQRISKRFELYIGGYEICNAYEEENDPVAQYHKFQAQKY 471
Query: 413 NR-AAGDDETPPTDEAFCTALEYGLPPTGG-GARVDRLTMFLTDSNNIKEVLLFPAMK 580
+R GDDETP D F ALEYGLPPT G G VDRL M +T + I E+L F +++
Sbjct: 472 DRLQLGDDETPAPDSDFVHALEYGLPPTAGWGMGVDRLVMLMTGQSKISEILPFGSLR 529
>SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 580
Score = 35.1 bits (77), Expect = 0.011
Identities = 28/118 (23%), Positives = 51/118 (43%), Gaps = 4/118 (3%)
Frame = +2
Query: 230 YKSDIYI--GYPQIMSPL-SKYHRDVPGLTERFEVYVAKKEICNAYTELNDPATQRERFE 400
Y +D Y+ YP + P + + P + ++ ++ +EI + ++DP ER +
Sbjct: 462 YHTDFYVIDKYPSSVRPFYTMPDPEDPRYSNSYDFFMKGQEIMSGAQRIHDPELLVERMK 521
Query: 401 EQAKNRAAGDDETPPTDEAFCTALEYGLPP-TGGGARVDRLTMFLTDSNNIKEVLLFP 571
+ G + + A G PP GGG ++R+ MF + NI+ FP
Sbjct: 522 ALGVSPDVGLQQ-------YIDAFAIGCPPHAGGGIGLERVVMFYLNLPNIRLASSFP 572
>SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 611
Score = 33.1 bits (72), Expect = 0.043
Identities = 19/43 (44%), Positives = 23/43 (53%), Gaps = 1/43 (2%)
Frame = +2
Query: 446 TDEAFCTALEYGLPPTGGGAR-VDRLTMFLTDSNNIKEVLLFP 571
T E L G PP GG A DRL LT++ I+EV+ FP
Sbjct: 533 TFEHLIRVLSSGCPPHGGIALGFDRLAALLTNAPGIREVIAFP 575
>SPCC1322.14c |vtc4||vacuolar transporter chaperone
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 721
Score = 28.7 bits (61), Expect = 0.92
Identities = 19/58 (32%), Positives = 30/58 (51%), Gaps = 3/58 (5%)
Frame = +3
Query: 6 RVEIDFTPPFARVP---MMATLERVLNVKLPAPDKLDTPEANDFLNNLCNTHQIECSP 170
R++I PF ++P ++ +L VKL D PE ++NNL N+H +E P
Sbjct: 395 RMDIGIDYPFDQLPDEDIVRFPYAILEVKLQTQFGQDPPE---WVNNLVNSHLVEAVP 449
>SPAC6F12.12 |par2|pbp2|protein phosphatase regulatory subunit
Par2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 627
Score = 27.5 bits (58), Expect = 2.1
Identities = 10/25 (40%), Positives = 17/25 (68%)
Frame = +2
Query: 308 TERFEVYVAKKEICNAYTELNDPAT 382
+ER E+++ K E CN + NDP++
Sbjct: 217 SEREELFIKKLEQCNIIFDFNDPSS 241
>SPCPB1C11.02 |||amino acid permease, unknown 16|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 505
Score = 27.5 bits (58), Expect = 2.1
Identities = 14/46 (30%), Positives = 22/46 (47%)
Frame = -2
Query: 523 SQTVYPSPTAGRRQAIF*SCTKRFIGWGSFIVTSSTILGLLFESFA 386
++ +Y +G +F C K I W S + TS+T + L S A
Sbjct: 334 TRLLYSLAKSGHAPKVFSKCNKHGIPWLSVLATSATAILCLMSSQA 379
>SPAP8A3.14c |||mitochondrial inner membrane protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 677
Score = 26.2 bits (55), Expect = 4.9
Identities = 11/19 (57%), Positives = 12/19 (63%)
Frame = +2
Query: 239 DIYIGYPQIMSPLSKYHRD 295
D YI Y I SPLS H+D
Sbjct: 621 DSYITYSDIFSPLSHSHKD 639
>SPBC16D10.05 |mok13||alpha-1,3-glucan synthase
Mok13|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2358
Score = 26.2 bits (55), Expect = 4.9
Identities = 15/33 (45%), Positives = 20/33 (60%)
Frame = +3
Query: 36 ARVPMMATLERVLNVKLPAPDKLDTPEANDFLN 134
AR P+ L++V KLP PD LDT + +D N
Sbjct: 1369 ARYPIFWGLKKV--GKLPNPDPLDTAQLDDPTN 1399
>SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 440
Score = 26.2 bits (55), Expect = 4.9
Identities = 11/29 (37%), Positives = 17/29 (58%)
Frame = -1
Query: 233 YTLLLQKYRNQFIQEPRGGPRWRALNLMS 147
Y+ L++KY FI++P W A + MS
Sbjct: 283 YSALIEKYPIVFIEDPFSEEDWGAFSYMS 311
>SPBC3B8.10c |||NLI interacting factor family|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 476
Score = 25.8 bits (54), Expect = 6.5
Identities = 12/40 (30%), Positives = 22/40 (55%)
Frame = -2
Query: 256 ISNINVGFIHSSSRNTETNLSKSLAVVLGGEHSI**VLHR 137
+ +++ IHS SR + T + + V + GEH I +H+
Sbjct: 307 VLDLDETLIHSVSRGSRTTSGQPIEVHVPGEHPILYYIHK 346
>SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 716
Score = 25.8 bits (54), Expect = 6.5
Identities = 11/31 (35%), Positives = 18/31 (58%)
Frame = -1
Query: 494 RSAAGHILELYKTLHRLGEFHRHQQHDSWLA 402
R A+G + L LH G+F + ++ +WLA
Sbjct: 592 RDASGKVTSLKLELHLDGDFKKTEKKVTWLA 622
>SPCC24B10.22 ||SPCPB16A4.01|mitochondrial DNA polymerase gamma
catalytic subunit|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1018
Score = 25.4 bits (53), Expect = 8.6
Identities = 13/41 (31%), Positives = 20/41 (48%)
Frame = -1
Query: 473 LELYKTLHRLGEFHRHQQHDSWLALRIFRVVSPGHLIRCRH 351
LE L +L E + HD++ ++F+ V P L C H
Sbjct: 336 LEKEPILQKLNELITYCAHDTYSTHQVFKKVFPQFLEVCPH 376
>SPBC1861.03 |mak10||NatC N-acetyltransferase complex subunit Mak10
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 708
Score = 25.4 bits (53), Expect = 8.6
Identities = 12/28 (42%), Positives = 18/28 (64%), Gaps = 2/28 (7%)
Frame = -3
Query: 594 VGSSGFIAGN--NNTSLILFESVRNIVK 517
VG SGF +G+ N T ++LF V ++K
Sbjct: 141 VGDSGFSSGSGRNTTDIVLFPFVLAVIK 168
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,346,917
Number of Sequences: 5004
Number of extensions: 75346
Number of successful extensions: 241
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 232
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 237
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 351258950
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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