Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00341
(787 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g16180.1 68414.m01938 TMS membrane family protein / tumour di... 41 8e-04
At3g06170.1 68416.m00709 TMS membrane family protein / tumour di... 34 0.12
At5g20610.1 68418.m02448 expressed protein 32 0.50
At5g58510.1 68418.m07327 expressed protein KIAA0066, Homo sapien... 31 0.87
At1g47290.2 68414.m05236 3-beta hydroxysteroid dehydrogenase/iso... 31 0.87
At1g47290.1 68414.m05235 3-beta hydroxysteroid dehydrogenase/iso... 31 0.87
At2g02160.1 68415.m00152 zinc finger (CCCH-type) family protein ... 31 1.1
At1g60630.1 68414.m06825 leucine-rich repeat family protein simi... 29 4.6
At5g62470.2 68418.m07840 myb family transcription factor (MYB96)... 28 6.1
At5g62470.1 68418.m07839 myb family transcription factor (MYB96)... 28 6.1
At1g50360.1 68414.m05645 myosin family protein contains Pfam pro... 28 6.1
At5g53620.2 68418.m06662 expressed protein 28 8.1
At5g53620.1 68418.m06661 expressed protein 28 8.1
At3g19960.1 68416.m02526 myosin (ATM) nearly identical to myosin... 28 8.1
At2g18690.1 68415.m02177 expressed protein 28 8.1
>At1g16180.1 68414.m01938 TMS membrane family protein / tumour
differentially expressed (TDE) family protein contains
Pfam domain, PF03348: TMS membrane protein/tumour
differentially expressed protein (TDE)
Length = 412
Score = 41.1 bits (92), Expect = 8e-04
Identities = 18/68 (26%), Positives = 32/68 (47%)
Frame = +2
Query: 509 DPRAGIQNGFWAIKYLLVXXXXXXXXXXXXXXXASTWMVFGMIGGFCYIVIQLILVIDFA 688
DPR GI +G W +K ++ S + G ++++Q++L++DF
Sbjct: 106 DPRDGIHHGGWMMK-IICWCILVIFMFFLPNEIISFYESMSKFGAGFFLLVQVVLLLDFV 164
Query: 689 HSWAEIWV 712
H W + WV
Sbjct: 165 HGWNDTWV 172
Score = 30.3 bits (65), Expect = 1.5
Identities = 12/27 (44%), Positives = 20/27 (74%)
Frame = +3
Query: 429 AVYRITFATCLFFLLMALIMIGVKSSK 509
AV R++ LFF +++++MIGVK+ K
Sbjct: 79 AVLRVSLGNFLFFSILSVMMIGVKNQK 105
>At3g06170.1 68416.m00709 TMS membrane family protein / tumour
differentially expressed (TDE) family protein contains
Pfam domain, PF03348: TMS membrane protein/tumour
differentially expressed protein (TDE)
Length = 409
Score = 33.9 bits (74), Expect = 0.12
Identities = 15/68 (22%), Positives = 30/68 (44%)
Frame = +2
Query: 509 DPRAGIQNGFWAIKYLLVXXXXXXXXXXXXXXXASTWMVFGMIGGFCYIVIQLILVIDFA 688
D R +G W +K ++V S + G ++++Q++L++D
Sbjct: 103 DRRDSWHHGGWGLK-MIVWFLLVVLMFFVPNVIVSLYGTLSKFGAGAFLLVQVVLLLDAT 161
Query: 689 HSWAEIWV 712
H+W + WV
Sbjct: 162 HNWNDSWV 169
Score = 33.1 bits (72), Expect = 0.21
Identities = 15/24 (62%), Positives = 18/24 (75%)
Frame = +3
Query: 429 AVYRITFATCLFFLLMALIMIGVK 500
AV R++F LFF + ALIMIGVK
Sbjct: 76 AVLRVSFGNFLFFAIYALIMIGVK 99
>At5g20610.1 68418.m02448 expressed protein
Length = 1164
Score = 31.9 bits (69), Expect = 0.50
Identities = 23/70 (32%), Positives = 33/70 (47%), Gaps = 2/70 (2%)
Frame = -1
Query: 241 WNWCKMDMLNNIVNKQ--CCHSNTLASSREVLFRKPCFQRYCTHVLTDASDPLISTLDAF 68
WNW + +N++ N++ CC S + S + P FQ V D +ST A
Sbjct: 62 WNW-PLRAINHVRNRRFNCCFSAQVHSIEGL---PPIFQDLSLTVHWKRRDESLSTRPAK 117
Query: 67 SSNTRSRFKD 38
SN R+ FKD
Sbjct: 118 VSNGRAEFKD 127
>At5g58510.1 68418.m07327 expressed protein KIAA0066, Homo sapiens,
EMBL:HSORFKG1O
Length = 963
Score = 31.1 bits (67), Expect = 0.87
Identities = 18/65 (27%), Positives = 32/65 (49%), Gaps = 1/65 (1%)
Frame = -1
Query: 691 MCKINDKNELDNNVAEASNHSKHHPCRSKLTSWDEECSD-NATNNQ*IFDGPKTILNSSS 515
M ++ N L+ + +A +H + +SW+ SD AT Q + DGPK ++ +
Sbjct: 1 MASVSKSNHLEED-DDAEEEVQHFDDFTLASSWERFISDIEATCRQWLADGPKNLVEKGA 59
Query: 514 WILED 500
+ED
Sbjct: 60 VAVED 64
>At1g47290.2 68414.m05236 3-beta hydroxysteroid
dehydrogenase/isomerase family protein contains Pfam
profile PF01073 3-beta hydroxysteroid
dehydrogenase/isomerase domain; similar to
NAD(P)-dependent steroid dehydrogenase from Homo sapiens
[SP|Q15738], Mus musculus [SP|Q9R1J0]
Length = 439
Score = 31.1 bits (67), Expect = 0.87
Identities = 19/60 (31%), Positives = 30/60 (50%), Gaps = 2/60 (3%)
Frame = -1
Query: 328 SSLCNPGAKVIQHTIVTSTSI--SAYIDGRRWNWCKMDMLNNIVNKQCCHSNTLASSREV 155
SS+ PG K++ ++VT+ S +I G N+ + N+V+ C LAS EV
Sbjct: 189 SSIFGPGDKLMVPSLVTAARAGKSKFIIGDGSNFYDFTYVENVVHAHVCAERALASGGEV 248
>At1g47290.1 68414.m05235 3-beta hydroxysteroid
dehydrogenase/isomerase family protein contains Pfam
profile PF01073 3-beta hydroxysteroid
dehydrogenase/isomerase domain; similar to
NAD(P)-dependent steroid dehydrogenase from Homo sapiens
[SP|Q15738], Mus musculus [SP|Q9R1J0]
Length = 382
Score = 31.1 bits (67), Expect = 0.87
Identities = 19/60 (31%), Positives = 30/60 (50%), Gaps = 2/60 (3%)
Frame = -1
Query: 328 SSLCNPGAKVIQHTIVTSTSI--SAYIDGRRWNWCKMDMLNNIVNKQCCHSNTLASSREV 155
SS+ PG K++ ++VT+ S +I G N+ + N+V+ C LAS EV
Sbjct: 189 SSIFGPGDKLMVPSLVTAARAGKSKFIIGDGSNFYDFTYVENVVHAHVCAERALASGGEV 248
>At2g02160.1 68415.m00152 zinc finger (CCCH-type) family protein
contains Pfam domain, PF00642: Zinc finger
C-x8-C-x5-C-x3-H type (and similar)
Length = 669
Score = 30.7 bits (66), Expect = 1.1
Identities = 19/67 (28%), Positives = 29/67 (43%)
Frame = -1
Query: 760 ISSAAYHPSILSLRI*NPYFSPGMCKINDKNELDNNVAEASNHSKHHPCRSKLTSWDEEC 581
I + A HPS+ + +F GMC D + A+ + K HP +K + D +C
Sbjct: 104 IHATAQHPSVAKQPVPCLFFQKGMCMKGDMCSFLHTPNPAA-YKKQHPVEAK-PATDPQC 161
Query: 580 SDNATNN 560
S N
Sbjct: 162 SKKPIEN 168
>At1g60630.1 68414.m06825 leucine-rich repeat family protein similar
to receptor kinase GI:498278 from [Petunia
integrifolia]; contains protein kinase domain,
Pfam:PF00069; contains leucine-rich repeats,
Pfam:PF00560
Length = 652
Score = 28.7 bits (61), Expect = 4.6
Identities = 17/43 (39%), Positives = 26/43 (60%), Gaps = 3/43 (6%)
Frame = +3
Query: 441 ITFATCLFFLLMALIMIG-VKSSKIQE-LEFKMVFGPSN-IYW 560
I+ ++C+FFL+ A +I V+SS ++ L K PSN I W
Sbjct: 2 ISSSSCMFFLVFAFFLISPVRSSDVEALLSLKSSIDPSNSIPW 44
>At5g62470.2 68418.m07840 myb family transcription factor (MYB96)
contains Pfam profile: PF00249 myb-like DNA-binding
domain
Length = 352
Score = 28.3 bits (60), Expect = 6.1
Identities = 32/113 (28%), Positives = 51/113 (45%), Gaps = 8/113 (7%)
Frame = -1
Query: 712 NPYFSPGMCKINDKNELDNNVAEASNHS--KHHPCRSKLTSW------DEECSDNATNNQ 557
N + + KIN+ E DN+ +SN S K+H +K W D + A
Sbjct: 109 NTHLKKKLKKINESGEEDNDGVSSSNTSSQKNHQSTNK-GQWERRLQTDINMAKQALCEA 167
Query: 556 *IFDGPKTILNSSSWILEDLTPIIINAISRKKRHVANVIL*TAKYPTASSQST 398
D P + L+SSS + TP+I + R+ ++ +L P++SS ST
Sbjct: 168 LSLDKPSSTLSSSSSL---PTPVI---TQQNIRNFSSALLDRCYDPSSSSSST 214
>At5g62470.1 68418.m07839 myb family transcription factor (MYB96)
contains Pfam profile: PF00249 myb-like DNA-binding
domain
Length = 351
Score = 28.3 bits (60), Expect = 6.1
Identities = 32/113 (28%), Positives = 51/113 (45%), Gaps = 8/113 (7%)
Frame = -1
Query: 712 NPYFSPGMCKINDKNELDNNVAEASNHS--KHHPCRSKLTSW------DEECSDNATNNQ 557
N + + KIN+ E DN+ +SN S K+H +K W D + A
Sbjct: 108 NTHLKKKLKKINESGEEDNDGVSSSNTSSQKNHQSTNK-GQWERRLQTDINMAKQALCEA 166
Query: 556 *IFDGPKTILNSSSWILEDLTPIIINAISRKKRHVANVIL*TAKYPTASSQST 398
D P + L+SSS + TP+I + R+ ++ +L P++SS ST
Sbjct: 167 LSLDKPSSTLSSSSSL---PTPVI---TQQNIRNFSSALLDRCYDPSSSSSST 213
>At1g50360.1 68414.m05645 myosin family protein contains Pfam
profiles: PF00063 myosin head (motor domain), PF00612 IQ
calmodulin-binding motif
Length = 1153
Score = 28.3 bits (60), Expect = 6.1
Identities = 16/40 (40%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
Frame = +3
Query: 144 FRNKTSRELASVLLWQHCLFTML-FSMSILHQFHLLPSMY 260
F +K +R +LL L S++ILHQF++LP MY
Sbjct: 768 FHHKFARRYGFLLLENIAAKDPLSVSVAILHQFNILPEMY 807
>At5g53620.2 68418.m06662 expressed protein
Length = 682
Score = 27.9 bits (59), Expect = 8.1
Identities = 14/29 (48%), Positives = 19/29 (65%)
Frame = -3
Query: 260 IHRREEVELVQDGHAEQHSEQAVLPQQHA 174
+H++E +LVQ QH EQ+VL QQ A
Sbjct: 388 MHQQEISQLVQPQVPSQHIEQSVLLQQKA 416
>At5g53620.1 68418.m06661 expressed protein
Length = 682
Score = 27.9 bits (59), Expect = 8.1
Identities = 14/29 (48%), Positives = 19/29 (65%)
Frame = -3
Query: 260 IHRREEVELVQDGHAEQHSEQAVLPQQHA 174
+H++E +LVQ QH EQ+VL QQ A
Sbjct: 388 MHQQEISQLVQPQVPSQHIEQSVLLQQKA 416
>At3g19960.1 68416.m02526 myosin (ATM) nearly identical to myosin
[Arabidopsis thaliana] GI:6491702; similar to myosin
GI:6491702 from [Arabidopsis thaliana] ;contains Pfam
profiles: PF00063: myosin head (motor domain), PF00612:
IQ calmodulin-binding motif; identical to cDNA myosin
(ATM) GI:297068
Length = 1166
Score = 27.9 bits (59), Expect = 8.1
Identities = 10/15 (66%), Positives = 14/15 (93%)
Frame = +3
Query: 216 SMSILHQFHLLPSMY 260
S++ILHQF++LP MY
Sbjct: 801 SVAILHQFNILPEMY 815
>At2g18690.1 68415.m02177 expressed protein
Length = 322
Score = 27.9 bits (59), Expect = 8.1
Identities = 9/38 (23%), Positives = 21/38 (55%)
Frame = +3
Query: 423 YLAVYRITFATCLFFLLMALIMIGVKSSKIQELEFKMV 536
Y+A++ + F F +L +++ +KS ++ + F V
Sbjct: 148 YIALFSLGFGFLFFIILCPILLFSIKSGSVENIGFLAV 185
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,229,215
Number of Sequences: 28952
Number of extensions: 336834
Number of successful extensions: 859
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 827
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 858
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1765546400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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