Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00336
(746 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g18040.1 68417.m02685 eukaryotic translation initiation facto... 84 8e-17
At5g35620.2 68418.m04252 eukaryotic translation initiation facto... 79 2e-15
At5g35620.1 68418.m04251 eukaryotic translation initiation facto... 79 2e-15
At5g18110.1 68418.m02126 novel cap-binding protein (nCBP) identi... 78 7e-15
At1g29590.1 68414.m03618 eukaryotic translation initiation facto... 73 2e-13
At1g29550.1 68414.m03614 eukaryotic translation initiation facto... 73 2e-13
>At4g18040.1 68417.m02685 eukaryotic translation initiation factor
4E 1 / eIF-4E1 / mRNA cap-binding protein 1 (EIF4E1)
identical to SP|O23252 Eukaryotic translation initiation
factor 4E (eIF-4E) (eIF4E) (mRNA cap-binding protein)
(eIF-4F 25 kDa subunit) (eIF-4F P26 subunit)
{Arabidopsis thaliana}
Length = 235
Score = 84.2 bits (199), Expect = 8e-17
Identities = 39/84 (46%), Positives = 54/84 (64%)
Frame = +1
Query: 256 SELRQGHDYAVFKQGIRPMWEDDANKMGGRWLISLEKKQRFTDLDRFWLDVVLLLIGENF 435
S+L G D+ FK I P WED GG+W ++ K++ D+ WL +L LIGE F
Sbjct: 109 SKLAHGADFYCFKHIIEPKWEDPICANGGKWTMTFPKEKS----DKSWLYTLLALIGEQF 164
Query: 436 ENSDEICGAVVNVRPKVDKIAIWT 507
++ DEICGAVVN+R K ++I+IWT
Sbjct: 165 DHGDEICGAVVNIRGKQERISIWT 188
Score = 47.6 bits (108), Expect = 9e-06
Identities = 20/48 (41%), Positives = 31/48 (64%), Gaps = 4/48 (8%)
Frame = +2
Query: 119 HPLQNQWSLWFYDN----DRNKTWEENLIELTTFDTVEDFWRLYHHIK 250
HPL++ W+ WF DN + +W +L + TF TVE+FW LY+++K
Sbjct: 60 HPLEHSWTFWF-DNPAVKSKQTSWGSSLRPVFTFSTVEEFWSLYNNMK 106
Score = 33.5 bits (73), Expect = 0.15
Identities = 18/46 (39%), Positives = 24/46 (52%)
Frame = +3
Query: 510 DAMKQHATIEIGKKLKEQLGIHGKIGFQVHRDTMVKHSSATKNLYT 647
+A + A + IGK+ KE L + IGF +H D K KN YT
Sbjct: 190 NASNEAAQVSIGKQWKEFLDYNNSIGFIIHEDAK-KLDRNAKNAYT 234
>At5g35620.2 68418.m04252 eukaryotic translation initiation factor
4E 2 / eIF-4E2 / mRNA cap-binding protein 2 (EIF4E2)
identical to SP|O04663 Eukaryotic translation initiation
factor 4E (eIF-4E) (eIF4E) (mRNA cap-binding protein)
(eIF-(iso)4F 25 kDa subunit) (eIF-(ISO)4F P28 subunit)
(eIF4Eiso protein) {Arabidopsis thaliana}
Length = 167
Score = 79.4 bits (187), Expect = 2e-15
Identities = 37/89 (41%), Positives = 57/89 (64%), Gaps = 4/89 (4%)
Frame = +1
Query: 253 TSELRQGHDYAVFKQGIRPMWEDDANKMGGRW--LISLEKKQRFTDLDRFWLDVVLLLIG 426
TS+L + +FK G+ P WED GG+W +++ +K+ LD+ WL+ ++ LIG
Sbjct: 72 TSKLTANAEIHLFKAGVEPKWEDPECANGGKWTWVVTANRKEA---LDKGWLETLMALIG 128
Query: 427 ENFENSDEICGAVVNVRP--KVDKIAIWT 507
E F+ +DEICG V +VRP K DK+++WT
Sbjct: 129 EQFDEADEICGVVASVRPQSKQDKLSLWT 157
Score = 46.8 bits (106), Expect = 2e-05
Identities = 22/45 (48%), Positives = 27/45 (60%), Gaps = 2/45 (4%)
Frame = +2
Query: 119 HPLQNQWSLWFYDNDRNK--TWEENLIELTTFDTVEDFWRLYHHI 247
H L+ +WS WF DN K W +L + TFDTVEDFW L+ I
Sbjct: 26 HKLERKWSFWF-DNQSKKGAAWGASLRKAYTFDTVEDFWGLHETI 69
Score = 29.1 bits (62), Expect = 3.3
Identities = 11/29 (37%), Positives = 15/29 (51%)
Frame = +1
Query: 313 WEDDANKMGGRWLISLEKKQRFTDLDRFW 399
W D+ +K G W SL K F ++ FW
Sbjct: 35 WFDNQSKKGAAWGASLRKAYTFDTVEDFW 63
>At5g35620.1 68418.m04251 eukaryotic translation initiation factor
4E 2 / eIF-4E2 / mRNA cap-binding protein 2 (EIF4E2)
identical to SP|O04663 Eukaryotic translation initiation
factor 4E (eIF-4E) (eIF4E) (mRNA cap-binding protein)
(eIF-(iso)4F 25 kDa subunit) (eIF-(ISO)4F P28 subunit)
(eIF4Eiso protein) {Arabidopsis thaliana}
Length = 198
Score = 79.4 bits (187), Expect = 2e-15
Identities = 37/89 (41%), Positives = 57/89 (64%), Gaps = 4/89 (4%)
Frame = +1
Query: 253 TSELRQGHDYAVFKQGIRPMWEDDANKMGGRW--LISLEKKQRFTDLDRFWLDVVLLLIG 426
TS+L + +FK G+ P WED GG+W +++ +K+ LD+ WL+ ++ LIG
Sbjct: 72 TSKLTANAEIHLFKAGVEPKWEDPECANGGKWTWVVTANRKEA---LDKGWLETLMALIG 128
Query: 427 ENFENSDEICGAVVNVRP--KVDKIAIWT 507
E F+ +DEICG V +VRP K DK+++WT
Sbjct: 129 EQFDEADEICGVVASVRPQSKQDKLSLWT 157
Score = 46.8 bits (106), Expect = 2e-05
Identities = 22/45 (48%), Positives = 27/45 (60%), Gaps = 2/45 (4%)
Frame = +2
Query: 119 HPLQNQWSLWFYDNDRNK--TWEENLIELTTFDTVEDFWRLYHHI 247
H L+ +WS WF DN K W +L + TFDTVEDFW L+ I
Sbjct: 26 HKLERKWSFWF-DNQSKKGAAWGASLRKAYTFDTVEDFWGLHETI 69
Score = 29.1 bits (62), Expect = 3.3
Identities = 11/29 (37%), Positives = 15/29 (51%)
Frame = +1
Query: 313 WEDDANKMGGRWLISLEKKQRFTDLDRFW 399
W D+ +K G W SL K F ++ FW
Sbjct: 35 WFDNQSKKGAAWGASLRKAYTFDTVEDFW 63
>At5g18110.1 68418.m02126 novel cap-binding protein (nCBP) identical
to novel cap-binding protein nCBP [Arabidopsis thaliana]
GI:3108209; contains Pfam profile PF01652: Eukaryotic
initiation factor 4E
Length = 221
Score = 77.8 bits (183), Expect = 7e-15
Identities = 35/76 (46%), Positives = 49/76 (64%)
Frame = +1
Query: 277 DYAVFKQGIRPMWEDDANKMGGRWLISLEKKQRFTDLDRFWLDVVLLLIGENFENSDEIC 456
D FK GIRP+WED AN GG+W+I K RFW D++L L+G+ +++D IC
Sbjct: 99 DLHFFKDGIRPLWEDGANCNGGKWIIRFSKVVSA----RFWEDLLLALVGDQLDDADNIC 154
Query: 457 GAVVNVRPKVDKIAIW 504
GAV++VR D I++W
Sbjct: 155 GAVLSVRFNEDIISVW 170
Score = 43.2 bits (97), Expect = 2e-04
Identities = 17/46 (36%), Positives = 30/46 (65%), Gaps = 3/46 (6%)
Frame = +2
Query: 119 HPLQNQWSLWFYDND---RNKTWEENLIELTTFDTVEDFWRLYHHI 247
HPL+ ++S+W+ RN+++E+N+ ++ F TVE FW Y H+
Sbjct: 43 HPLRYKFSIWYTRRTPGVRNQSYEDNIKKMVEFSTVEGFWACYCHL 88
>At1g29590.1 68414.m03618 eukaryotic translation initiation factor
4E, putative / eIF-4E, putative / eIF4E, putative / mRNA
cap-binding protein, putative similar to SP|O23252
Eukaryotic translation initiation factor 4E (eIF-4E)
(eIF4E) (mRNA cap-binding protein) (eIF-4F 25 kDa
subunit) (eIF-4F P26 subunit) {Arabidopsis thaliana};
contains Pfam profile PF01652: Eukaryotic initiation
factor 4E
Length = 285
Score = 73.3 bits (172), Expect = 2e-13
Identities = 33/79 (41%), Positives = 46/79 (58%)
Frame = +1
Query: 271 GHDYAVFKQGIRPMWEDDANKMGGRWLISLEKKQRFTDLDRFWLDVVLLLIGENFENSDE 450
G D FK I P WED GG+W + + L+ WL+ +L L+GE F+ DE
Sbjct: 164 GSDLYCFKDKIEPKWEDPICANGGKWTMFFPR----ATLESNWLNTLLALVGEQFDQGDE 219
Query: 451 ICGAVVNVRPKVDKIAIWT 507
ICGAV+N R + D+I++WT
Sbjct: 220 ICGAVLNFRTRGDRISLWT 238
Score = 46.0 bits (104), Expect = 3e-05
Identities = 21/47 (44%), Positives = 29/47 (61%), Gaps = 4/47 (8%)
Frame = +2
Query: 119 HPLQNQWSLWFYDNDRNKT----WEENLIELTTFDTVEDFWRLYHHI 247
H QN W+ WF DN +K+ W +L L TF T+E+FW LY++I
Sbjct: 110 HCFQNSWTFWF-DNPSSKSNQVIWGSSLRSLYTFATIEEFWSLYNNI 155
Score = 35.5 bits (78), Expect = 0.038
Identities = 20/46 (43%), Positives = 24/46 (52%)
Frame = +3
Query: 513 AMKQHATIEIGKKLKEQLGIHGKIGFQVHRDTMVKHSSATKNLYTV 650
A + A + IGK+ KE LG + IGF VH D A K YTV
Sbjct: 241 AANEEAQLSIGKQWKELLGYNDTIGFIVHEDAKTLDRDA-KRRYTV 285
>At1g29550.1 68414.m03614 eukaryotic translation initiation factor
4E, putative / eIF-4E, putative / eIF4E, putative / mRNA
cap-binding protein, putative similar to SP|O23252
Eukaryotic translation initiation factor 4E (eIF-4E)
(eIF4E) (mRNA cap-binding protein) (eIF-4F 25 kDa
subunit) (eIF-4F P26 subunit) {Arabidopsis thaliana};
contains Pfam profile PF01652: Eukaryotic initiation
factor 4E
Length = 240
Score = 73.3 bits (172), Expect = 2e-13
Identities = 34/79 (43%), Positives = 46/79 (58%)
Frame = +1
Query: 271 GHDYAVFKQGIRPMWEDDANKMGGRWLISLEKKQRFTDLDRFWLDVVLLLIGENFENSDE 450
G D FK I P WED GG+W + K L+ WL+ +L L+GE F+ DE
Sbjct: 119 GADLYCFKDKIEPKWEDPICANGGKWSMMFPK----ATLECNWLNTLLALVGEQFDQGDE 174
Query: 451 ICGAVVNVRPKVDKIAIWT 507
ICGAV+N R + D+I++WT
Sbjct: 175 ICGAVLNFRARGDRISLWT 193
Score = 46.4 bits (105), Expect = 2e-05
Identities = 21/47 (44%), Positives = 29/47 (61%), Gaps = 4/47 (8%)
Frame = +2
Query: 119 HPLQNQWSLWFYDNDRNKT----WEENLIELTTFDTVEDFWRLYHHI 247
H QN W+ WF DN +K+ W +L L TF T+E+FW LY++I
Sbjct: 65 HCFQNSWTFWF-DNPSSKSNQVIWGSSLRSLYTFGTIEEFWSLYNNI 110
Score = 35.5 bits (78), Expect = 0.038
Identities = 20/47 (42%), Positives = 25/47 (53%)
Frame = +3
Query: 510 DAMKQHATIEIGKKLKEQLGIHGKIGFQVHRDTMVKHSSATKNLYTV 650
+A + A + IGK+ KE LG + IGF VH D A K YTV
Sbjct: 195 NAANEEAQLSIGKQWKELLGYNETIGFIVHEDAKTLDRDA-KRRYTV 240
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,631,320
Number of Sequences: 28952
Number of extensions: 314510
Number of successful extensions: 797
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 756
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 786
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1653386488
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -