BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00330 (751 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces pomb... 54 3e-08 SPAC57A7.09 |||human RNF family homolog|Schizosaccharomyces pomb... 54 3e-08 SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 50 3e-07 SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 48 1e-06 SPAC3A12.03c |mug145||ubiquitin-protein ligase E3 |Schizosacchar... 48 2e-06 SPCC126.07c |||human CTD-binding SR-like protein rA9 homolog|Sch... 46 4e-06 SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 46 8e-06 SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3 |Schizos... 45 1e-05 SPBC17D11.02c |||synoviolin homolog|Schizosaccharomyces pombe|ch... 44 2e-05 SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 44 3e-05 SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 42 9e-05 SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8 |Schizosacch... 39 7e-04 SPBC16G5.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 39 9e-04 SPAC23H4.18c |rbx1|pip1, pip1|RING-box protein 1|Schizosaccharom... 36 0.008 SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3 |Schi... 35 0.014 SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 35 0.014 SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 34 0.025 SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 33 0.043 SPAC2F3.16 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 33 0.057 SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces pom... 32 0.076 SPAC17G8.10c |dma1||mitotic spindle checkpoint protein Dma1|Schi... 32 0.10 SPAC343.03 |apc11||anaphase-promoting complex subunit Apc11|Schi... 31 0.23 SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo... 30 0.31 SPCC31H12.07 |sec231|sec23a, SPCC5E4.01|GTPase activating protei... 27 3.8 SPAC16A10.06c |nse2||Smc5-6 complex non-SMC subunit 2 |Schizosac... 26 5.0 SPCC330.07c |||membrane transporter|Schizosaccharomyces pombe|ch... 26 6.6 SPBC1734.06 |rhp18||Rad18 homolog Rhp18|Schizosaccharomyces pomb... 26 6.6 SPAC1002.16c |||nicotinic acid plasma membrane transporter |Schi... 26 6.6 SPAC22E12.11c |set3||histone lysine methyltransferase Set3|Schiz... 25 8.7 >SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces pombe|chr 2|||Manual Length = 741 Score = 53.6 bits (123), Expect = 3e-08 Identities = 23/48 (47%), Positives = 29/48 (60%), Gaps = 2/48 (4%) Frame = +2 Query: 299 CPICLKNL-EAGEK-VKKMPCNHIFHPRCILTWLDKTNSCPFCRHEMP 436 CPIC ++ E EK KMPC HIF C+ WL+ +CP CR E+P Sbjct: 107 CPICYDDMNENDEKQATKMPCGHIFGKNCLQKWLENHCTCPLCRKEVP 154 >SPAC57A7.09 |||human RNF family homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 372 Score = 53.6 bits (123), Expect = 3e-08 Identities = 21/47 (44%), Positives = 29/47 (61%), Gaps = 1/47 (2%) Frame = +2 Query: 299 CPICLKNLEAGEKVKKMPCNHIFHPRCILTWL-DKTNSCPFCRHEMP 436 C ICL++ G+KV +PC H FH CI W+ D ++CP C E+P Sbjct: 321 CVICLESFTKGDKVVALPCKHEFHRPCIAKWIVDYRHACPTCNTEVP 367 >SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 583 Score = 50.4 bits (115), Expect = 3e-07 Identities = 20/44 (45%), Positives = 27/44 (61%), Gaps = 2/44 (4%) Frame = +2 Query: 299 CPICLKNLEAGEKVKKMP-CNHIFHPRCILTWLDKT-NSCPFCR 424 C +CL N E ++ +++ CNH FH CI WL + NSCP CR Sbjct: 525 CLVCLSNFELNDECRRLKQCNHFFHRECIDQWLTSSQNSCPLCR 568 >SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 513 Score = 48.4 bits (110), Expect = 1e-06 Identities = 19/57 (33%), Positives = 31/57 (54%) Frame = +2 Query: 254 IQKLQDVVIENQKKGCPICLKNLEAGEKVKKMPCNHIFHPRCILTWLDKTNSCPFCR 424 +QK +I+ + + C IC++ + + V ++PC H FH CI WL +C CR Sbjct: 382 VQKPPKELIDEEGE-CTICMEMFKINDDVIQLPCKHYFHENCIKPWLRVNGTCAICR 437 >SPAC3A12.03c |mug145||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 309 Score = 47.6 bits (108), Expect = 2e-06 Identities = 17/53 (32%), Positives = 31/53 (58%), Gaps = 1/53 (1%) Frame = +2 Query: 275 VIENQKKGCPICLKNLEAGEKVKKMPCNHIFHPRCILTWLDKTN-SCPFCRHE 430 ++E+++ C IC + + ++ +PC H+FH +CI TW+ SCP C + Sbjct: 197 LLEDEEDFCIICYADYAFDDILRVLPCEHVFHTQCIDTWMTTMKASCPLCNED 249 >SPCC126.07c |||human CTD-binding SR-like protein rA9 homolog|Schizosaccharomyces pombe|chr 3|||Manual Length = 571 Score = 46.4 bits (105), Expect = 4e-06 Identities = 16/43 (37%), Positives = 24/43 (55%) Frame = +2 Query: 302 PICLKNLEAGEKVKKMPCNHIFHPRCILTWLDKTNSCPFCRHE 430 P + + G ++ K+PC H FH C+ +W N+CP CR E Sbjct: 39 PASISSTLDGLRIAKIPCGHYFHNHCLESWCRVANTCPLCRTE 81 >SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 821 Score = 45.6 bits (103), Expect = 8e-06 Identities = 23/53 (43%), Positives = 28/53 (52%), Gaps = 3/53 (5%) Frame = +2 Query: 299 CPICLKNLEAGEKVKKMP-CNHIFHPRCILTWLDK-TNSCPFCR-HEMPTDDE 448 C ICL+ G+ +K+ C H FH CI WL NSCP CR H + T E Sbjct: 766 CLICLETYTNGDICRKLQACKHFFHQACIDQWLTTGNNSCPLCRAHGVTTQAE 818 >SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 556 Score = 45.2 bits (102), Expect = 1e-05 Identities = 21/56 (37%), Positives = 32/56 (57%), Gaps = 2/56 (3%) Frame = +2 Query: 290 KKGCPICL-KNLEAGEKVKKMPCNHIFHPRCILTWLDKTNS-CPFCRHEMPTDDEN 451 ++ C ICL + E +++PC+HIFHP CI +L K + CP C+ + EN Sbjct: 495 QRECTICLCEYSEESPLYRELPCHHIFHPACIDPYLLKNSDLCPLCKQSVTNMLEN 550 >SPBC17D11.02c |||synoviolin homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 677 Score = 44.4 bits (100), Expect = 2e-05 Identities = 13/31 (41%), Positives = 20/31 (64%) Frame = +2 Query: 341 KKMPCNHIFHPRCILTWLDKTNSCPFCRHEM 433 K++PC HI H C+ WL++ +CP CR + Sbjct: 324 KRLPCGHILHFHCLRNWLERQQTCPICRRSV 354 >SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 676 Score = 43.6 bits (98), Expect = 3e-05 Identities = 12/29 (41%), Positives = 21/29 (72%) Frame = +2 Query: 350 PCNHIFHPRCILTWLDKTNSCPFCRHEMP 436 PC+H++H +C+L W++ + CP CR +P Sbjct: 646 PCHHLYHRQCLLQWMETRSICPVCRCHLP 674 >SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 468 Score = 41.9 bits (94), Expect = 9e-05 Identities = 18/47 (38%), Positives = 26/47 (55%) Frame = +2 Query: 299 CPICLKNLEAGEKVKKMPCNHIFHPRCILTWLDKTNSCPFCRHEMPT 439 CPIC EA ++ C H + C+L WL ++ SCP CR ++ T Sbjct: 85 CPICT---EALQRPFTTHCGHTYCYECLLNWLKESKSCPTCRQKLYT 128 >SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8 |Schizosaccharomyces pombe|chr 2|||Manual Length = 269 Score = 39.1 bits (87), Expect = 7e-04 Identities = 24/71 (33%), Positives = 36/71 (50%), Gaps = 3/71 (4%) Frame = +2 Query: 230 TSACIERGIQKLQDVVIENQKKGCPICLKNLEAGEKVKKMPCNHIFHPRCILTWLD---K 400 T+A IE+ + VV +Q+ C+ L++ E + PC HIF CIL+ L Sbjct: 180 TNAEIEKEEPSKKQVVPSSQRLADYKCVICLDSPENLSCTPCGHIFCNFCILSALGTTAA 239 Query: 401 TNSCPFCRHEM 433 T CP CR ++ Sbjct: 240 TQKCPVCRRKV 250 >SPBC16G5.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 268 Score = 38.7 bits (86), Expect = 9e-04 Identities = 16/49 (32%), Positives = 28/49 (57%), Gaps = 1/49 (2%) Frame = +2 Query: 290 KKGCPICLKNLEAGEKVKKMPCNHI-FHPRCILTWLDKTNSCPFCRHEM 433 ++ C ICL+ + G + + PC H F CI W+D++ +CP C+ + Sbjct: 18 RRDCVICLQ--KDGLRAQLSPCGHDQFDYSCICRWMDQSLTCPICKRHV 64 >SPAC23H4.18c |rbx1|pip1, pip1|RING-box protein 1|Schizosaccharomyces pombe|chr 1|||Manual Length = 107 Score = 35.5 bits (78), Expect = 0.008 Identities = 13/26 (50%), Positives = 14/26 (53%) Frame = +2 Query: 353 CNHIFHPRCILTWLDKTNSCPFCRHE 430 CNH FH CI WL+ N CP E Sbjct: 74 CNHAFHFHCISRWLNTRNVCPLDNRE 99 >SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 306 Score = 34.7 bits (76), Expect = 0.014 Identities = 16/48 (33%), Positives = 23/48 (47%) Frame = +2 Query: 281 ENQKKGCPICLKNLEAGEKVKKMPCNHIFHPRCILTWLDKTNSCPFCR 424 E +K C +C++ + + C HIF CI W K + CP CR Sbjct: 251 EGNRK-CSLCMEFIHCPAATE---CGHIFCWSCINGWTSKKSECPLCR 294 >SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 547 Score = 34.7 bits (76), Expect = 0.014 Identities = 14/44 (31%), Positives = 24/44 (54%), Gaps = 1/44 (2%) Frame = +2 Query: 299 CPICLKNLEAG-EKVKKMPCNHIFHPRCILTWLDKTNSCPFCRH 427 C +CL+ +++ + + C H FH C+ W +SCP CR+ Sbjct: 208 CVVCLERMDSSITGLITIVCQHTFHCPCLQKW--GNSSCPVCRY 249 >SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1610 Score = 33.9 bits (74), Expect = 0.025 Identities = 16/47 (34%), Positives = 19/47 (40%), Gaps = 5/47 (10%) Frame = +2 Query: 299 CPICLKNLEAGEKVKKM---PCNHIFHPRCILTWLDKTNS--CPFCR 424 C IC L + C H FH C+ W +NS CP CR Sbjct: 1558 CAICYSVLSVERTLPNKRCGTCRHKFHASCLYKWFKSSNSSRCPLCR 1604 >SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 486 Score = 33.1 bits (72), Expect = 0.043 Identities = 19/61 (31%), Positives = 31/61 (50%), Gaps = 2/61 (3%) Frame = +2 Query: 263 LQDVVIENQKKG--CPICLKNLEAGEKVKKMPCNHIFHPRCILTWLDKTNSCPFCRHEMP 436 ++D++ EN + C IC L + V PC H F C++ L ++ CP CR +P Sbjct: 155 MEDLLQENVSRELECQICFGMLY--DPVVS-PCGHTFCGPCLMQALTQSPQCPTCRFGLP 211 Query: 437 T 439 + Sbjct: 212 S 212 >SPAC2F3.16 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 425 Score = 32.7 bits (71), Expect = 0.057 Identities = 16/67 (23%), Positives = 28/67 (41%), Gaps = 1/67 (1%) Frame = +2 Query: 224 KLTSACIERGIQKLQDVVIENQKKGCPICLKNL-EAGEKVKKMPCNHIFHPRCILTWLDK 400 K C+ + + + CPIC + + + E+V + C+H H RC ++ Sbjct: 246 KTCGLCLPISVFNTHRCIERSTDCNCPICGEYMFNSRERVIFLSCSHPLHQRCHEEYIRT 305 Query: 401 TNSCPFC 421 CP C Sbjct: 306 NYRCPTC 312 >SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces pombe|chr 1|||Manual Length = 1375 Score = 32.3 bits (70), Expect = 0.076 Identities = 9/27 (33%), Positives = 17/27 (62%) Frame = +2 Query: 353 CNHIFHPRCILTWLDKTNSCPFCRHEM 433 C H++ C+ WL ++SCP C+ ++ Sbjct: 1107 CGHLYCSFCLEAWLKHSSSCPMCKTKL 1133 >SPAC17G8.10c |dma1||mitotic spindle checkpoint protein Dma1|Schizosaccharomyces pombe|chr 1|||Manual Length = 267 Score = 31.9 bits (69), Expect = 0.10 Identities = 18/63 (28%), Positives = 35/63 (55%), Gaps = 7/63 (11%) Frame = +2 Query: 257 QKLQDVVI----ENQKKGCPICLKNLEAGEKVKKMPCNHIFHPRCILTWLDKTN---SCP 415 +++Q++V+ E+ C ICL + + + PC+H +H +CI L++++ SC Sbjct: 174 KRMQELVLCGSSESGPPECCICLMPVLPCQALFVAPCSHSYHYKCIRPTLNESHPYFSCF 233 Query: 416 FCR 424 CR Sbjct: 234 ICR 236 >SPAC343.03 |apc11||anaphase-promoting complex subunit Apc11|Schizosaccharomyces pombe|chr 1|||Manual Length = 94 Score = 30.7 bits (66), Expect = 0.23 Identities = 14/34 (41%), Positives = 15/34 (44%), Gaps = 3/34 (8%) Frame = +2 Query: 353 CNHIFHPRCILTWLDKTNS---CPFCRHEMPTDD 445 C HIFH CI WL + S CP R D Sbjct: 52 CKHIFHAHCIQNWLATSGSQGQCPMDRQTFVVAD 85 >SPCC18.03 |||shuttle craft like transcriptional regulator|Schizosaccharomyces pombe|chr 3|||Manual Length = 1077 Score = 30.3 bits (65), Expect = 0.31 Identities = 15/60 (25%), Positives = 27/60 (45%), Gaps = 9/60 (15%) Frame = +2 Query: 272 VVIENQKKGCPICLKNLEAGEKVKKM-PCNHIFHPRCILTW----LDKTNS----CPFCR 424 V ++N+ C +C + + C H+FH CI W +++ N CP+C+ Sbjct: 188 VELKNRLYECSVCTDTINPSTSIWSCGTCYHVFHLSCIRKWCKNSIEQRNEDAWRCPYCQ 247 >SPCC31H12.07 |sec231|sec23a, SPCC5E4.01|GTPase activating protein Sec23a|Schizosaccharomyces pombe|chr 3|||Manual Length = 759 Score = 26.6 bits (56), Expect = 3.8 Identities = 10/26 (38%), Positives = 13/26 (50%) Frame = +2 Query: 344 KMPCNHIFHPRCILTWLDKTNSCPFC 421 K PC + +P C + K CPFC Sbjct: 57 KAPCKAVLNPYCHIDTRAKFWICPFC 82 >SPAC16A10.06c |nse2||Smc5-6 complex non-SMC subunit 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 250 Score = 26.2 bits (55), Expect = 5.0 Identities = 12/39 (30%), Positives = 19/39 (48%) Frame = +2 Query: 299 CPICLKNLEAGEKVKKMPCNHIFHPRCILTWLDKTNSCP 415 CP+ L+ + + CNH + IL+ L+ T CP Sbjct: 179 CPLTLQPIV--HPILSTACNHFYEKDAILSLLNPTCVCP 215 >SPCC330.07c |||membrane transporter|Schizosaccharomyces pombe|chr 3|||Manual Length = 500 Score = 25.8 bits (54), Expect = 6.6 Identities = 14/28 (50%), Positives = 18/28 (64%) Frame = -3 Query: 482 LVSLLSCRLHNFRHQ*ASHVYKTGMSLS 399 L+SL S +LH H AS V + G+SLS Sbjct: 427 LISLSSKQLHPRHHNTASIVVQVGVSLS 454 >SPBC1734.06 |rhp18||Rad18 homolog Rhp18|Schizosaccharomyces pombe|chr 2|||Manual Length = 387 Score = 25.8 bits (54), Expect = 6.6 Identities = 10/24 (41%), Positives = 13/24 (54%) Frame = +2 Query: 353 CNHIFHPRCILTWLDKTNSCPFCR 424 C+H F CI +L + CP CR Sbjct: 44 CSHTFCSFCIRDYLREHPMCPACR 67 >SPAC1002.16c |||nicotinic acid plasma membrane transporter |Schizosaccharomyces pombe|chr 1|||Manual Length = 499 Score = 25.8 bits (54), Expect = 6.6 Identities = 12/38 (31%), Positives = 21/38 (55%) Frame = +2 Query: 599 LWLSCLMFIYNNFLLKHVHNVKFLSFLILHTVD*SSTP 712 +++S ++ + N F LKH ++ K S T+D S P Sbjct: 450 IFISTVLHVVNIFFLKHQNSKKQKSLASSSTIDLSEQP 487 >SPAC22E12.11c |set3||histone lysine methyltransferase Set3|Schizosaccharomyces pombe|chr 1|||Manual Length = 859 Score = 25.4 bits (53), Expect = 8.7 Identities = 11/37 (29%), Positives = 17/37 (45%) Frame = +2 Query: 302 PICLKNLEAGEKVKKMPCNHIFHPRCILTWLDKTNSC 412 P + GE + + + + HPR LDKT+ C Sbjct: 557 PTTPPSFNVGESLSRRSASPLQHPRTSPDMLDKTSPC 593 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,133,720 Number of Sequences: 5004 Number of extensions: 65089 Number of successful extensions: 193 Number of sequences better than 10.0: 29 Number of HSP's better than 10.0 without gapping: 181 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 193 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 357280532 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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