SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00327
         (508 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC1685.02c |rps1202|rps12-2|40S ribosomal protein S12|Schizosa...    80   2e-16
SPCC962.04 |rps1201|rps12-1, rps12|40S ribosomal protein S12|Sch...    78   7e-16
SPBC106.13 |||conserved eukaryotic protein|Schizosaccharomyces p...    27   1.6  
SPBC713.02c |ubp21|ubpD, ubp15|ubiquitin C-terminal hydrolase Ub...    25   4.9  
SPAPJ696.01c |vps17||retromer complex subunit Vps17|Schizosaccha...    25   6.5  
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac...    25   8.6  
SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch...    25   8.6  
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon...    25   8.6  
SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ...    25   8.6  
SPBC2G2.08 |ade9||C-1-tetrahydrofolatesynthase/methylenetetrahyd...    25   8.6  

>SPBC1685.02c |rps1202|rps12-2|40S ribosomal protein
           S12|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 148

 Score = 79.8 bits (188), Expect = 2e-16
 Identities = 34/54 (62%), Positives = 42/54 (77%)
 Frame = +2

Query: 260 EHQIPLVKVDNNKKLGEWAGLCKIDKDGKARKIVGCSCVVIKDFGEETPALDVL 421
           E Q PLVKV + K LGEWAGLC +D+DG ARK+VGCSCV + D+GE++ AL  L
Sbjct: 89  ESQTPLVKVADPKILGEWAGLCVLDRDGNARKVVGCSCVAVTDYGEDSVALQTL 142



 Score = 76.2 bits (179), Expect = 3e-15
 Identities = 35/57 (61%), Positives = 45/57 (78%)
 Frame = +3

Query: 84  MDVNVALQEVLKTALIHGGLVHGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQAL 254
           + V  +L+EVLK AL+H GL  G+ EA+KALD+RQA LCVL E+CD+ AY KLV+AL
Sbjct: 30  LSVEDSLKEVLKRALVHDGLARGIREASKALDRRQAHLCVLCESCDQEAYVKLVEAL 86


>SPCC962.04 |rps1201|rps12-1, rps12|40S ribosomal protein
           S12|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 145

 Score = 78.2 bits (184), Expect = 7e-16
 Identities = 32/54 (59%), Positives = 42/54 (77%)
 Frame = +2

Query: 260 EHQIPLVKVDNNKKLGEWAGLCKIDKDGKARKIVGCSCVVIKDFGEETPALDVL 421
           E + PL+KV + K LGEWAGLC +D+DG ARK+VGCSCV + D+GE++ AL  L
Sbjct: 86  ESETPLIKVADPKVLGEWAGLCVLDRDGNARKVVGCSCVAVTDYGEDSAALQKL 139



 Score = 77.0 bits (181), Expect = 2e-15
 Identities = 36/57 (63%), Positives = 45/57 (78%)
 Frame = +3

Query: 84  MDVNVALQEVLKTALIHGGLVHGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQAL 254
           + V  AL+EVLK AL+H GL  G+ EA+KALD+RQA LCVL E+CD+ AY KLV+AL
Sbjct: 27  VSVEDALKEVLKRALVHDGLARGIREASKALDRRQAHLCVLCESCDQEAYVKLVEAL 83


>SPBC106.13 |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 404

 Score = 27.1 bits (57), Expect = 1.6
 Identities = 10/25 (40%), Positives = 14/25 (56%)
 Frame = -1

Query: 409 RWSFLAEIFDDNTGAADNFPCLAIL 335
           RW +LA +F  N  A  N P + +L
Sbjct: 257 RWEYLASLFTSNFTAVHNIPSVPLL 281


>SPBC713.02c |ubp21|ubpD, ubp15|ubiquitin C-terminal hydrolase
           Ubp21|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1129

 Score = 25.4 bits (53), Expect = 4.9
 Identities = 14/38 (36%), Positives = 20/38 (52%)
 Frame = +3

Query: 45  VEVPTNPILSGNNMDVNVALQEVLKTALIHGGLVHGLH 158
           +E+   P LS    D + +   VL   L+HGG +HG H
Sbjct: 445 LEIDLEPYLS-ETADKSESHVYVLHGVLVHGGDLHGGH 481


>SPAPJ696.01c |vps17||retromer complex subunit
           Vps17|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 549

 Score = 25.0 bits (52), Expect = 6.5
 Identities = 10/28 (35%), Positives = 20/28 (71%), Gaps = 1/28 (3%)
 Frame = -3

Query: 377 QHRSSRQFSLPCHPCQSCRD-QPILQAF 297
           + ++ +QF LP  PCQ+  + +PI+++F
Sbjct: 226 KRKALKQFPLPPDPCQALANLRPIVKSF 253


>SPCP31B10.07 |eft202||translation elongation factor 2
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 842

 Score = 24.6 bits (51), Expect = 8.6
 Identities = 15/55 (27%), Positives = 24/55 (43%)
 Frame = -2

Query: 234 CMRLHHSSQPKHIVLLASCQEL*RLREDRAQDHHGLKLSLAPLAVLHSRPCCYPT 70
           C+    S   +HIV  A    L    +D  +DH G+ L ++P  V +      P+
Sbjct: 517 CVLCTTSESGEHIVAGAGELHLEICLKDLQEDHAGIPLKISPPVVSYRESVSEPS 571


>SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1133

 Score = 24.6 bits (51), Expect = 8.6
 Identities = 9/20 (45%), Positives = 14/20 (70%)
 Frame = -2

Query: 141 DHHGLKLSLAPLAVLHSRPC 82
           D  GL  ++  L+++HSRPC
Sbjct: 529 DEMGLGKTIEVLSLIHSRPC 548


>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
           elongation factor 2 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 842

 Score = 24.6 bits (51), Expect = 8.6
 Identities = 15/55 (27%), Positives = 24/55 (43%)
 Frame = -2

Query: 234 CMRLHHSSQPKHIVLLASCQEL*RLREDRAQDHHGLKLSLAPLAVLHSRPCCYPT 70
           C+    S   +HIV  A    L    +D  +DH G+ L ++P  V +      P+
Sbjct: 517 CVLCTTSESGEHIVAGAGELHLEICLKDLQEDHAGIPLKISPPVVSYRESVSEPS 571


>SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 821

 Score = 24.6 bits (51), Expect = 8.6
 Identities = 11/32 (34%), Positives = 14/32 (43%)
 Frame = +3

Query: 219 DEAAYKKLVQALATNIRFHWSRLTTTKSLENG 314
           DE      V  L   ++  W R TT  S E+G
Sbjct: 14  DEPTTSSRVSMLVDRVKLLWKRYTTPLSTEDG 45


>SPBC2G2.08 |ade9||C-1-
           tetrahydrofolatesynthase/methylenetetrahydrofolatedehydr
           ogenase/methylenetetrahydrofolatecyclohydrolase/formylte
           trahydrofolatesynthetase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 969

 Score = 24.6 bits (51), Expect = 8.6
 Identities = 11/20 (55%), Positives = 15/20 (75%)
 Frame = +3

Query: 351 GKLSAAPVLSSKISARKLQR 410
           G  S+A +L+ K+ ARKLQR
Sbjct: 28  GTSSSANILNGKLLARKLQR 47


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,031,173
Number of Sequences: 5004
Number of extensions: 39025
Number of successful extensions: 130
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 125
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 130
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 202220600
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -