BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00310
(742 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 78 1e-15
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 53 4e-08
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 42 9e-05
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 40 3e-04
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 36 0.005
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 35 0.014
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 31 0.23
SPCC16C4.07 |scw1||RNA-binding protein Scw1|Schizosaccharomyces ... 30 0.40
SPAC823.03 |ppk15||serine/threonine protein kinase Ppk15 |Schizo... 29 0.92
SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr ... 27 2.8
SPBC13G1.10c |mug81||ATP-dependent RNA helicase Slh1|Schizosacch... 27 3.7
SPCC1259.10 |pgp1||metallopeptidase Pgp1|Schizosaccharomyces pom... 27 3.7
SPAC4F10.16c |||P-type ATPase |Schizosaccharomyces pombe|chr 1||... 27 3.7
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 26 4.9
SPAC167.05 ||SPAC57A7.01|Usp |Schizosaccharomyces pombe|chr 1|||... 26 4.9
SPBC2A9.09 |||phosducin family protein|Schizosaccharomyces pombe... 26 4.9
SPAC19G12.07c |rsd1||RNA-binding protein Rsd1|Schizosaccharomyce... 26 6.5
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 78.2 bits (184), Expect = 1e-15
Identities = 53/148 (35%), Positives = 69/148 (46%), Gaps = 7/148 (4%)
Frame = +3
Query: 198 PFFRAATSKVSESSI--SQSRVDRCADTASAQGVSAMPTFIFYRNRTKIDRLEGGNTTVL 371
P F SK + ++ VD AS GV AMPTF+F+ N +ID L G N L
Sbjct: 39 PLFSQLASKYASPKFVFAKVNVDEQRQIASGLGVKAMPTFVFFENGKQIDMLTGANPQAL 98
Query: 372 ENKV----RQYYGSDVAEEDEDNTVAGHMDLITFITKSECECLNEADDHPLPKH*QMTGD 539
+ KV + G+ V G L I + ECLN+ DDH L
Sbjct: 99 KEKVALISSKATGTGALASSSSAPVKGFASLQGCIENPQLECLNQQDDHDLKSAFNSNPS 158
Query: 540 T-LASYCDEQLIINISFNQLVKLHSIKI 620
+ L S DEQL+I I F ++VK+HSI I
Sbjct: 159 SFLESDVDEQLMIYIPFLEVVKVHSIAI 186
Score = 41.1 bits (92), Expect = 2e-04
Identities = 21/55 (38%), Positives = 33/55 (60%), Gaps = 2/55 (3%)
Frame = +1
Query: 94 IQNDAHFQTEMANAGTKLVVVDFTATWCPPCQRIAPFFEQLPAKF--PRAVFLKV 252
I++ H+ + + +G + VD A WC PC+ I+P F QL +K+ P+ VF KV
Sbjct: 6 IRSYQHWISTIPKSG--YLAVDCYADWCGPCKAISPLFSQLASKYASPKFVFAKV 58
Score = 34.7 bits (76), Expect = 0.014
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = +2
Query: 620 QGPADKGPKSIKLFINQPRTLDFDQAAGYS 709
+G PK+IKL+INQP L F+ A ++
Sbjct: 190 KGETSSAPKTIKLYINQPNNLSFEDAESFT 219
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 53.2 bits (122), Expect = 4e-08
Identities = 28/58 (48%), Positives = 33/58 (56%)
Frame = +1
Query: 142 KLVVVDFTATWCPPCQRIAPFFEQLPAKFPRAVFLKVE*TDAPIQRVLRASVQCPHSF 315
KLVVVDF ATWC PC+ IAP FEQ + A F+KV+ D + A V SF
Sbjct: 19 KLVVVDFFATWCGPCKAIAPKFEQFSNTYSDATFIKVD-VDQLSEIAAEAGVHAMPSF 75
Score = 44.8 bits (101), Expect = 1e-05
Identities = 20/63 (31%), Positives = 36/63 (57%)
Frame = +3
Query: 198 PFFRAATSKVSESSISQSRVDRCADTASAQGVSAMPTFIFYRNRTKIDRLEGGNTTVLEN 377
P F ++ S+++ + VD+ ++ A+ GV AMP+F Y+N KI+ + G N LE
Sbjct: 38 PKFEQFSNTYSDATFIKVDVDQLSEIAAEAGVHAMPSFFLYKNGEKIEEIVGANPAKLEA 97
Query: 378 KVR 386
++
Sbjct: 98 SIK 100
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 41.9 bits (94), Expect = 9e-05
Identities = 17/37 (45%), Positives = 24/37 (64%)
Frame = +1
Query: 142 KLVVVDFTATWCPPCQRIAPFFEQLPAKFPRAVFLKV 252
K+ VVDF A WC PC+ + PF E+L + +A F+ V
Sbjct: 36 KVTVVDFYADWCGPCKYLKPFLEKLSEQNQKASFIAV 72
Score = 40.7 bits (91), Expect = 2e-04
Identities = 17/65 (26%), Positives = 33/65 (50%)
Frame = +3
Query: 198 PFFRAATSKVSESSISQSRVDRCADTASAQGVSAMPTFIFYRNRTKIDRLEGGNTTVLEN 377
PF + + ++S D+ +D A GV A+PT + +R ++DR+ G + L +
Sbjct: 55 PFLEKLSEQNQKASFIAVNADKFSDIAQKNGVYALPTMVLFRKGQELDRIVGADVKTLSS 114
Query: 378 KVRQY 392
+ +Y
Sbjct: 115 LLAKY 119
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 40.3 bits (90), Expect = 3e-04
Identities = 16/48 (33%), Positives = 28/48 (58%)
Frame = +1
Query: 112 FQTEMANAGTKLVVVDFTATWCPPCQRIAPFFEQLPAKFPRAVFLKVE 255
FQ + N ++++++F A W PC+++ F+Q AVFLK+E
Sbjct: 11 FQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAKDTKNAVFLKIE 58
Score = 26.6 bits (56), Expect = 3.7
Identities = 11/45 (24%), Positives = 23/45 (51%)
Frame = +3
Query: 258 DRCADTASAQGVSAMPTFIFYRNRTKIDRLEGGNTTVLENKVRQY 392
++ +D A + V+A+P F+ + R+ G N L+ + +Y
Sbjct: 60 EKFSDIAESFDVNAVPLFVLIHGAKVLARISGANPQKLKAAIDEY 104
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 36.3 bits (80), Expect = 0.005
Identities = 14/32 (43%), Positives = 21/32 (65%)
Frame = +1
Query: 133 AGTKLVVVDFTATWCPPCQRIAPFFEQLPAKF 228
A K +++F ATWC C+ +AP +E+L A F
Sbjct: 37 ASKKGALIEFYATWCGHCKSLAPVYEELGALF 68
Score = 33.1 bits (72), Expect = 0.043
Identities = 14/29 (48%), Positives = 19/29 (65%)
Frame = +1
Query: 142 KLVVVDFTATWCPPCQRIAPFFEQLPAKF 228
K V+V+F A WC C+R+AP +E L F
Sbjct: 159 KDVLVEFYADWCGYCKRLAPTYETLGKVF 187
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 34.7 bits (76), Expect = 0.014
Identities = 13/30 (43%), Positives = 21/30 (70%)
Frame = +1
Query: 139 TKLVVVDFTATWCPPCQRIAPFFEQLPAKF 228
TK V+V+F A WC C+ +AP +E+L ++
Sbjct: 373 TKDVLVEFYAPWCGHCKNLAPTYEKLAEEY 402
Score = 28.7 bits (61), Expect = 0.92
Identities = 10/23 (43%), Positives = 16/23 (69%)
Frame = +1
Query: 142 KLVVVDFTATWCPPCQRIAPFFE 210
K+++V F A WC C+ +AP +E
Sbjct: 40 KVLMVKFYAPWCGHCKALAPEYE 62
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 30.7 bits (66), Expect = 0.23
Identities = 15/46 (32%), Positives = 25/46 (54%)
Frame = +1
Query: 79 GLATVIQNDAHFQTEMANAGTKLVVVDFTATWCPPCQRIAPFFEQL 216
G T+ N +F+ + G LVV F A WC C+++ P +++L
Sbjct: 30 GSNTIELNSKNFRKFVKAKGPSLVV--FYAPWCGYCKKLVPTYQKL 73
>SPCC16C4.07 |scw1||RNA-binding protein Scw1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 561
Score = 29.9 bits (64), Expect = 0.40
Identities = 15/33 (45%), Positives = 18/33 (54%)
Frame = -3
Query: 344 SVDFGPVSVENECGHCTDALSTRCIGASVYSTL 246
SV+ PVSV+NE H S+R AS TL
Sbjct: 138 SVELNPVSVDNEVAHGLAVFSSRDAAASARDTL 170
>SPAC823.03 |ppk15||serine/threonine protein kinase Ppk15
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 534
Score = 28.7 bits (61), Expect = 0.92
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = -2
Query: 195 NPLARWTPSCCKVHND*LGSSVSHFSLE 112
NPL RWTP K H G+S S + ++
Sbjct: 442 NPLKRWTPDQAKNHPFITGASFSQYCID 469
>SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1337
Score = 27.1 bits (57), Expect = 2.8
Identities = 13/26 (50%), Positives = 15/26 (57%)
Frame = +3
Query: 432 VAGHMDLITFITKSECECLNEADDHP 509
V H+D I FITKS C + E D P
Sbjct: 1285 VVNHIDRIMFITKSLCGEIFEIDKWP 1310
>SPBC13G1.10c |mug81||ATP-dependent RNA helicase
Slh1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1935
Score = 26.6 bits (56), Expect = 3.7
Identities = 13/25 (52%), Positives = 18/25 (72%)
Frame = +2
Query: 170 LGVHRAKGLPLFSSSYQQSFREQYF 244
LGV+R KGL FSS+++ EQ+F
Sbjct: 471 LGVNRYKGLFYFSSAFRPCPIEQHF 495
>SPCC1259.10 |pgp1||metallopeptidase Pgp1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 412
Score = 26.6 bits (56), Expect = 3.7
Identities = 13/53 (24%), Positives = 24/53 (45%)
Frame = -3
Query: 344 SVDFGPVSVENECGHCTDALSTRCIGASVYSTLRNTALGNFAGSCSKKGAILW 186
S+DF +++ GH S + + T + A+G++ C+K I W
Sbjct: 170 SIDFPYLNILVSGGHTMLVYSNSLLNHEIIVTTSDIAVGDYLDKCAKYLGIPW 222
>SPAC4F10.16c |||P-type ATPase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1367
Score = 26.6 bits (56), Expect = 3.7
Identities = 10/17 (58%), Positives = 13/17 (76%)
Frame = -1
Query: 655 LYRFGSFVSWTLIFIEC 605
+++FG FVSWT IEC
Sbjct: 1196 VFKFGDFVSWTGRNIEC 1212
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 26.2 bits (55), Expect = 4.9
Identities = 13/51 (25%), Positives = 24/51 (47%)
Frame = +3
Query: 213 ATSKVSESSISQSRVDRCADTASAQGVSAMPTFIFYRNRTKIDRLEGGNTT 365
A +V S+ VD + +S + A+PT Y+N ++ + G +T
Sbjct: 71 AKEQVEGSNFHFGEVDCSKELSSCANIRAVPTLYLYQNGEIVEEVPFGAST 121
>SPAC167.05 ||SPAC57A7.01|Usp |Schizosaccharomyces pombe|chr
1|||Manual
Length = 601
Score = 26.2 bits (55), Expect = 4.9
Identities = 10/30 (33%), Positives = 18/30 (60%)
Frame = +3
Query: 357 NTTVLENKVRQYYGSDVAEEDEDNTVAGHM 446
NT+ + + + +YGSD+ E ++D A M
Sbjct: 366 NTSFVSSNINNWYGSDLEELEQDVKTAKSM 395
>SPBC2A9.09 |||phosducin family protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 233
Score = 26.2 bits (55), Expect = 4.9
Identities = 13/48 (27%), Positives = 25/48 (52%), Gaps = 1/48 (2%)
Frame = +1
Query: 112 FQTEMANAGTKL-VVVDFTATWCPPCQRIAPFFEQLPAKFPRAVFLKV 252
+ E+ +A ++ VVV P C+ +A E+L +P+ F+K+
Sbjct: 97 YTAEVTDASKEVFVVVHMFQDSLPACKLLAAILERLAPMYPQIKFVKI 144
>SPAC19G12.07c |rsd1||RNA-binding protein Rsd1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 604
Score = 25.8 bits (54), Expect = 6.5
Identities = 12/23 (52%), Positives = 13/23 (56%)
Frame = +2
Query: 239 YFSK*SRQMRRYSECSGRQCNAH 307
Y S+ SR RRY E S R AH
Sbjct: 180 YSSRHSRSRRRYEERSSRSSRAH 202
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,811,039
Number of Sequences: 5004
Number of extensions: 54324
Number of successful extensions: 167
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 151
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 165
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 351258950
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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