SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00310
         (742 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac...    78   1e-15
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc...    53   4e-08
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha...    42   9e-05
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c...    40   3e-04
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ...    36   0.005
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po...    35   0.014
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po...    31   0.23 
SPCC16C4.07 |scw1||RNA-binding protein Scw1|Schizosaccharomyces ...    30   0.40 
SPAC823.03 |ppk15||serine/threonine protein kinase Ppk15 |Schizo...    29   0.92 
SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr ...    27   2.8  
SPBC13G1.10c |mug81||ATP-dependent RNA helicase Slh1|Schizosacch...    27   3.7  
SPCC1259.10 |pgp1||metallopeptidase Pgp1|Schizosaccharomyces pom...    27   3.7  
SPAC4F10.16c |||P-type ATPase |Schizosaccharomyces pombe|chr 1||...    27   3.7  
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ...    26   4.9  
SPAC167.05 ||SPAC57A7.01|Usp |Schizosaccharomyces pombe|chr 1|||...    26   4.9  
SPBC2A9.09 |||phosducin family protein|Schizosaccharomyces pombe...    26   4.9  
SPAC19G12.07c |rsd1||RNA-binding protein Rsd1|Schizosaccharomyce...    26   6.5  

>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
           Txl1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 290

 Score = 78.2 bits (184), Expect = 1e-15
 Identities = 53/148 (35%), Positives = 69/148 (46%), Gaps = 7/148 (4%)
 Frame = +3

Query: 198 PFFRAATSKVSESSI--SQSRVDRCADTASAQGVSAMPTFIFYRNRTKIDRLEGGNTTVL 371
           P F    SK +      ++  VD     AS  GV AMPTF+F+ N  +ID L G N   L
Sbjct: 39  PLFSQLASKYASPKFVFAKVNVDEQRQIASGLGVKAMPTFVFFENGKQIDMLTGANPQAL 98

Query: 372 ENKV----RQYYGSDVAEEDEDNTVAGHMDLITFITKSECECLNEADDHPLPKH*QMTGD 539
           + KV     +  G+          V G   L   I   + ECLN+ DDH L         
Sbjct: 99  KEKVALISSKATGTGALASSSSAPVKGFASLQGCIENPQLECLNQQDDHDLKSAFNSNPS 158

Query: 540 T-LASYCDEQLIINISFNQLVKLHSIKI 620
           + L S  DEQL+I I F ++VK+HSI I
Sbjct: 159 SFLESDVDEQLMIYIPFLEVVKVHSIAI 186



 Score = 41.1 bits (92), Expect = 2e-04
 Identities = 21/55 (38%), Positives = 33/55 (60%), Gaps = 2/55 (3%)
 Frame = +1

Query: 94  IQNDAHFQTEMANAGTKLVVVDFTATWCPPCQRIAPFFEQLPAKF--PRAVFLKV 252
           I++  H+ + +  +G   + VD  A WC PC+ I+P F QL +K+  P+ VF KV
Sbjct: 6   IRSYQHWISTIPKSG--YLAVDCYADWCGPCKAISPLFSQLASKYASPKFVFAKV 58



 Score = 34.7 bits (76), Expect = 0.014
 Identities = 13/30 (43%), Positives = 19/30 (63%)
 Frame = +2

Query: 620 QGPADKGPKSIKLFINQPRTLDFDQAAGYS 709
           +G     PK+IKL+INQP  L F+ A  ++
Sbjct: 190 KGETSSAPKTIKLYINQPNNLSFEDAESFT 219


>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 103

 Score = 53.2 bits (122), Expect = 4e-08
 Identities = 28/58 (48%), Positives = 33/58 (56%)
 Frame = +1

Query: 142 KLVVVDFTATWCPPCQRIAPFFEQLPAKFPRAVFLKVE*TDAPIQRVLRASVQCPHSF 315
           KLVVVDF ATWC PC+ IAP FEQ    +  A F+KV+  D   +    A V    SF
Sbjct: 19  KLVVVDFFATWCGPCKAIAPKFEQFSNTYSDATFIKVD-VDQLSEIAAEAGVHAMPSF 75



 Score = 44.8 bits (101), Expect = 1e-05
 Identities = 20/63 (31%), Positives = 36/63 (57%)
 Frame = +3

Query: 198 PFFRAATSKVSESSISQSRVDRCADTASAQGVSAMPTFIFYRNRTKIDRLEGGNTTVLEN 377
           P F   ++  S+++  +  VD+ ++ A+  GV AMP+F  Y+N  KI+ + G N   LE 
Sbjct: 38  PKFEQFSNTYSDATFIKVDVDQLSEIAAEAGVHAMPSFFLYKNGEKIEEIVGANPAKLEA 97

Query: 378 KVR 386
            ++
Sbjct: 98  SIK 100


>SPBC12D12.07c |trx2||mitochondrial thioredoxin
           Trx2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 121

 Score = 41.9 bits (94), Expect = 9e-05
 Identities = 17/37 (45%), Positives = 24/37 (64%)
 Frame = +1

Query: 142 KLVVVDFTATWCPPCQRIAPFFEQLPAKFPRAVFLKV 252
           K+ VVDF A WC PC+ + PF E+L  +  +A F+ V
Sbjct: 36  KVTVVDFYADWCGPCKYLKPFLEKLSEQNQKASFIAV 72



 Score = 40.7 bits (91), Expect = 2e-04
 Identities = 17/65 (26%), Positives = 33/65 (50%)
 Frame = +3

Query: 198 PFFRAATSKVSESSISQSRVDRCADTASAQGVSAMPTFIFYRNRTKIDRLEGGNTTVLEN 377
           PF    + +  ++S      D+ +D A   GV A+PT + +R   ++DR+ G +   L +
Sbjct: 55  PFLEKLSEQNQKASFIAVNADKFSDIAQKNGVYALPTMVLFRKGQELDRIVGADVKTLSS 114

Query: 378 KVRQY 392
            + +Y
Sbjct: 115 LLAKY 119


>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 244

 Score = 40.3 bits (90), Expect = 3e-04
 Identities = 16/48 (33%), Positives = 28/48 (58%)
 Frame = +1

Query: 112 FQTEMANAGTKLVVVDFTATWCPPCQRIAPFFEQLPAKFPRAVFLKVE 255
           FQ  + N   ++++++F A W  PC+++   F+Q       AVFLK+E
Sbjct: 11  FQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAKDTKNAVFLKIE 58



 Score = 26.6 bits (56), Expect = 3.7
 Identities = 11/45 (24%), Positives = 23/45 (51%)
 Frame = +3

Query: 258 DRCADTASAQGVSAMPTFIFYRNRTKIDRLEGGNTTVLENKVRQY 392
           ++ +D A +  V+A+P F+       + R+ G N   L+  + +Y
Sbjct: 60  EKFSDIAESFDVNAVPLFVLIHGAKVLARISGANPQKLKAAIDEY 104


>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 359

 Score = 36.3 bits (80), Expect = 0.005
 Identities = 14/32 (43%), Positives = 21/32 (65%)
 Frame = +1

Query: 133 AGTKLVVVDFTATWCPPCQRIAPFFEQLPAKF 228
           A  K  +++F ATWC  C+ +AP +E+L A F
Sbjct: 37  ASKKGALIEFYATWCGHCKSLAPVYEELGALF 68



 Score = 33.1 bits (72), Expect = 0.043
 Identities = 14/29 (48%), Positives = 19/29 (65%)
 Frame = +1

Query: 142 KLVVVDFTATWCPPCQRIAPFFEQLPAKF 228
           K V+V+F A WC  C+R+AP +E L   F
Sbjct: 159 KDVLVEFYADWCGYCKRLAPTYETLGKVF 187


>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 492

 Score = 34.7 bits (76), Expect = 0.014
 Identities = 13/30 (43%), Positives = 21/30 (70%)
 Frame = +1

Query: 139 TKLVVVDFTATWCPPCQRIAPFFEQLPAKF 228
           TK V+V+F A WC  C+ +AP +E+L  ++
Sbjct: 373 TKDVLVEFYAPWCGHCKNLAPTYEKLAEEY 402



 Score = 28.7 bits (61), Expect = 0.92
 Identities = 10/23 (43%), Positives = 16/23 (69%)
 Frame = +1

Query: 142 KLVVVDFTATWCPPCQRIAPFFE 210
           K+++V F A WC  C+ +AP +E
Sbjct: 40  KVLMVKFYAPWCGHCKALAPEYE 62


>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 363

 Score = 30.7 bits (66), Expect = 0.23
 Identities = 15/46 (32%), Positives = 25/46 (54%)
 Frame = +1

Query: 79  GLATVIQNDAHFQTEMANAGTKLVVVDFTATWCPPCQRIAPFFEQL 216
           G  T+  N  +F+  +   G  LVV  F A WC  C+++ P +++L
Sbjct: 30  GSNTIELNSKNFRKFVKAKGPSLVV--FYAPWCGYCKKLVPTYQKL 73


>SPCC16C4.07 |scw1||RNA-binding protein Scw1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 561

 Score = 29.9 bits (64), Expect = 0.40
 Identities = 15/33 (45%), Positives = 18/33 (54%)
 Frame = -3

Query: 344 SVDFGPVSVENECGHCTDALSTRCIGASVYSTL 246
           SV+  PVSV+NE  H     S+R   AS   TL
Sbjct: 138 SVELNPVSVDNEVAHGLAVFSSRDAAASARDTL 170


>SPAC823.03 |ppk15||serine/threonine protein kinase Ppk15
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 534

 Score = 28.7 bits (61), Expect = 0.92
 Identities = 12/28 (42%), Positives = 16/28 (57%)
 Frame = -2

Query: 195 NPLARWTPSCCKVHND*LGSSVSHFSLE 112
           NPL RWTP   K H    G+S S + ++
Sbjct: 442 NPLKRWTPDQAKNHPFITGASFSQYCID 469


>SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1337

 Score = 27.1 bits (57), Expect = 2.8
 Identities = 13/26 (50%), Positives = 15/26 (57%)
 Frame = +3

Query: 432  VAGHMDLITFITKSECECLNEADDHP 509
            V  H+D I FITKS C  + E D  P
Sbjct: 1285 VVNHIDRIMFITKSLCGEIFEIDKWP 1310


>SPBC13G1.10c |mug81||ATP-dependent RNA helicase
           Slh1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1935

 Score = 26.6 bits (56), Expect = 3.7
 Identities = 13/25 (52%), Positives = 18/25 (72%)
 Frame = +2

Query: 170 LGVHRAKGLPLFSSSYQQSFREQYF 244
           LGV+R KGL  FSS+++    EQ+F
Sbjct: 471 LGVNRYKGLFYFSSAFRPCPIEQHF 495


>SPCC1259.10 |pgp1||metallopeptidase Pgp1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 412

 Score = 26.6 bits (56), Expect = 3.7
 Identities = 13/53 (24%), Positives = 24/53 (45%)
 Frame = -3

Query: 344 SVDFGPVSVENECGHCTDALSTRCIGASVYSTLRNTALGNFAGSCSKKGAILW 186
           S+DF  +++    GH     S   +   +  T  + A+G++   C+K   I W
Sbjct: 170 SIDFPYLNILVSGGHTMLVYSNSLLNHEIIVTTSDIAVGDYLDKCAKYLGIPW 222


>SPAC4F10.16c |||P-type ATPase |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1367

 Score = 26.6 bits (56), Expect = 3.7
 Identities = 10/17 (58%), Positives = 13/17 (76%)
 Frame = -1

Query: 655  LYRFGSFVSWTLIFIEC 605
            +++FG FVSWT   IEC
Sbjct: 1196 VFKFGDFVSWTGRNIEC 1212


>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 726

 Score = 26.2 bits (55), Expect = 4.9
 Identities = 13/51 (25%), Positives = 24/51 (47%)
 Frame = +3

Query: 213 ATSKVSESSISQSRVDRCADTASAQGVSAMPTFIFYRNRTKIDRLEGGNTT 365
           A  +V  S+     VD   + +S   + A+PT   Y+N   ++ +  G +T
Sbjct: 71  AKEQVEGSNFHFGEVDCSKELSSCANIRAVPTLYLYQNGEIVEEVPFGAST 121


>SPAC167.05 ||SPAC57A7.01|Usp |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 601

 Score = 26.2 bits (55), Expect = 4.9
 Identities = 10/30 (33%), Positives = 18/30 (60%)
 Frame = +3

Query: 357 NTTVLENKVRQYYGSDVAEEDEDNTVAGHM 446
           NT+ + + +  +YGSD+ E ++D   A  M
Sbjct: 366 NTSFVSSNINNWYGSDLEELEQDVKTAKSM 395


>SPBC2A9.09 |||phosducin family protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 233

 Score = 26.2 bits (55), Expect = 4.9
 Identities = 13/48 (27%), Positives = 25/48 (52%), Gaps = 1/48 (2%)
 Frame = +1

Query: 112 FQTEMANAGTKL-VVVDFTATWCPPCQRIAPFFEQLPAKFPRAVFLKV 252
           +  E+ +A  ++ VVV       P C+ +A   E+L   +P+  F+K+
Sbjct: 97  YTAEVTDASKEVFVVVHMFQDSLPACKLLAAILERLAPMYPQIKFVKI 144


>SPAC19G12.07c |rsd1||RNA-binding protein Rsd1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 604

 Score = 25.8 bits (54), Expect = 6.5
 Identities = 12/23 (52%), Positives = 13/23 (56%)
 Frame = +2

Query: 239 YFSK*SRQMRRYSECSGRQCNAH 307
           Y S+ SR  RRY E S R   AH
Sbjct: 180 YSSRHSRSRRRYEERSSRSSRAH 202


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,811,039
Number of Sequences: 5004
Number of extensions: 54324
Number of successful extensions: 167
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 151
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 165
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 351258950
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -