BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00310 (742 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 78 1e-15 SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 53 4e-08 SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 42 9e-05 SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 40 3e-04 SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 36 0.005 SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 35 0.014 SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 31 0.23 SPCC16C4.07 |scw1||RNA-binding protein Scw1|Schizosaccharomyces ... 30 0.40 SPAC823.03 |ppk15||serine/threonine protein kinase Ppk15 |Schizo... 29 0.92 SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr ... 27 2.8 SPBC13G1.10c |mug81||ATP-dependent RNA helicase Slh1|Schizosacch... 27 3.7 SPCC1259.10 |pgp1||metallopeptidase Pgp1|Schizosaccharomyces pom... 27 3.7 SPAC4F10.16c |||P-type ATPase |Schizosaccharomyces pombe|chr 1||... 27 3.7 SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 26 4.9 SPAC167.05 ||SPAC57A7.01|Usp |Schizosaccharomyces pombe|chr 1|||... 26 4.9 SPBC2A9.09 |||phosducin family protein|Schizosaccharomyces pombe... 26 4.9 SPAC19G12.07c |rsd1||RNA-binding protein Rsd1|Schizosaccharomyce... 26 6.5 >SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosaccharomyces pombe|chr 2|||Manual Length = 290 Score = 78.2 bits (184), Expect = 1e-15 Identities = 53/148 (35%), Positives = 69/148 (46%), Gaps = 7/148 (4%) Frame = +3 Query: 198 PFFRAATSKVSESSI--SQSRVDRCADTASAQGVSAMPTFIFYRNRTKIDRLEGGNTTVL 371 P F SK + ++ VD AS GV AMPTF+F+ N +ID L G N L Sbjct: 39 PLFSQLASKYASPKFVFAKVNVDEQRQIASGLGVKAMPTFVFFENGKQIDMLTGANPQAL 98 Query: 372 ENKV----RQYYGSDVAEEDEDNTVAGHMDLITFITKSECECLNEADDHPLPKH*QMTGD 539 + KV + G+ V G L I + ECLN+ DDH L Sbjct: 99 KEKVALISSKATGTGALASSSSAPVKGFASLQGCIENPQLECLNQQDDHDLKSAFNSNPS 158 Query: 540 T-LASYCDEQLIINISFNQLVKLHSIKI 620 + L S DEQL+I I F ++VK+HSI I Sbjct: 159 SFLESDVDEQLMIYIPFLEVVKVHSIAI 186 Score = 41.1 bits (92), Expect = 2e-04 Identities = 21/55 (38%), Positives = 33/55 (60%), Gaps = 2/55 (3%) Frame = +1 Query: 94 IQNDAHFQTEMANAGTKLVVVDFTATWCPPCQRIAPFFEQLPAKF--PRAVFLKV 252 I++ H+ + + +G + VD A WC PC+ I+P F QL +K+ P+ VF KV Sbjct: 6 IRSYQHWISTIPKSG--YLAVDCYADWCGPCKAISPLFSQLASKYASPKFVFAKV 58 Score = 34.7 bits (76), Expect = 0.014 Identities = 13/30 (43%), Positives = 19/30 (63%) Frame = +2 Query: 620 QGPADKGPKSIKLFINQPRTLDFDQAAGYS 709 +G PK+IKL+INQP L F+ A ++ Sbjct: 190 KGETSSAPKTIKLYINQPNNLSFEDAESFT 219 >SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 103 Score = 53.2 bits (122), Expect = 4e-08 Identities = 28/58 (48%), Positives = 33/58 (56%) Frame = +1 Query: 142 KLVVVDFTATWCPPCQRIAPFFEQLPAKFPRAVFLKVE*TDAPIQRVLRASVQCPHSF 315 KLVVVDF ATWC PC+ IAP FEQ + A F+KV+ D + A V SF Sbjct: 19 KLVVVDFFATWCGPCKAIAPKFEQFSNTYSDATFIKVD-VDQLSEIAAEAGVHAMPSF 75 Score = 44.8 bits (101), Expect = 1e-05 Identities = 20/63 (31%), Positives = 36/63 (57%) Frame = +3 Query: 198 PFFRAATSKVSESSISQSRVDRCADTASAQGVSAMPTFIFYRNRTKIDRLEGGNTTVLEN 377 P F ++ S+++ + VD+ ++ A+ GV AMP+F Y+N KI+ + G N LE Sbjct: 38 PKFEQFSNTYSDATFIKVDVDQLSEIAAEAGVHAMPSFFLYKNGEKIEEIVGANPAKLEA 97 Query: 378 KVR 386 ++ Sbjct: 98 SIK 100 >SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccharomyces pombe|chr 2|||Manual Length = 121 Score = 41.9 bits (94), Expect = 9e-05 Identities = 17/37 (45%), Positives = 24/37 (64%) Frame = +1 Query: 142 KLVVVDFTATWCPPCQRIAPFFEQLPAKFPRAVFLKV 252 K+ VVDF A WC PC+ + PF E+L + +A F+ V Sbjct: 36 KVTVVDFYADWCGPCKYLKPFLEKLSEQNQKASFIAV 72 Score = 40.7 bits (91), Expect = 2e-04 Identities = 17/65 (26%), Positives = 33/65 (50%) Frame = +3 Query: 198 PFFRAATSKVSESSISQSRVDRCADTASAQGVSAMPTFIFYRNRTKIDRLEGGNTTVLEN 377 PF + + ++S D+ +D A GV A+PT + +R ++DR+ G + L + Sbjct: 55 PFLEKLSEQNQKASFIAVNADKFSDIAQKNGVYALPTMVLFRKGQELDRIVGADVKTLSS 114 Query: 378 KVRQY 392 + +Y Sbjct: 115 LLAKY 119 >SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr 2|||Manual Length = 244 Score = 40.3 bits (90), Expect = 3e-04 Identities = 16/48 (33%), Positives = 28/48 (58%) Frame = +1 Query: 112 FQTEMANAGTKLVVVDFTATWCPPCQRIAPFFEQLPAKFPRAVFLKVE 255 FQ + N ++++++F A W PC+++ F+Q AVFLK+E Sbjct: 11 FQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAKDTKNAVFLKIE 58 Score = 26.6 bits (56), Expect = 3.7 Identities = 11/45 (24%), Positives = 23/45 (51%) Frame = +3 Query: 258 DRCADTASAQGVSAMPTFIFYRNRTKIDRLEGGNTTVLENKVRQY 392 ++ +D A + V+A+P F+ + R+ G N L+ + +Y Sbjct: 60 EKFSDIAESFDVNAVPLFVLIHGAKVLARISGANPQKLKAAIDEY 104 >SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 359 Score = 36.3 bits (80), Expect = 0.005 Identities = 14/32 (43%), Positives = 21/32 (65%) Frame = +1 Query: 133 AGTKLVVVDFTATWCPPCQRIAPFFEQLPAKF 228 A K +++F ATWC C+ +AP +E+L A F Sbjct: 37 ASKKGALIEFYATWCGHCKSLAPVYEELGALF 68 Score = 33.1 bits (72), Expect = 0.043 Identities = 14/29 (48%), Positives = 19/29 (65%) Frame = +1 Query: 142 KLVVVDFTATWCPPCQRIAPFFEQLPAKF 228 K V+V+F A WC C+R+AP +E L F Sbjct: 159 KDVLVEFYADWCGYCKRLAPTYETLGKVF 187 >SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 492 Score = 34.7 bits (76), Expect = 0.014 Identities = 13/30 (43%), Positives = 21/30 (70%) Frame = +1 Query: 139 TKLVVVDFTATWCPPCQRIAPFFEQLPAKF 228 TK V+V+F A WC C+ +AP +E+L ++ Sbjct: 373 TKDVLVEFYAPWCGHCKNLAPTYEKLAEEY 402 Score = 28.7 bits (61), Expect = 0.92 Identities = 10/23 (43%), Positives = 16/23 (69%) Frame = +1 Query: 142 KLVVVDFTATWCPPCQRIAPFFE 210 K+++V F A WC C+ +AP +E Sbjct: 40 KVLMVKFYAPWCGHCKALAPEYE 62 >SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 363 Score = 30.7 bits (66), Expect = 0.23 Identities = 15/46 (32%), Positives = 25/46 (54%) Frame = +1 Query: 79 GLATVIQNDAHFQTEMANAGTKLVVVDFTATWCPPCQRIAPFFEQL 216 G T+ N +F+ + G LVV F A WC C+++ P +++L Sbjct: 30 GSNTIELNSKNFRKFVKAKGPSLVV--FYAPWCGYCKKLVPTYQKL 73 >SPCC16C4.07 |scw1||RNA-binding protein Scw1|Schizosaccharomyces pombe|chr 3|||Manual Length = 561 Score = 29.9 bits (64), Expect = 0.40 Identities = 15/33 (45%), Positives = 18/33 (54%) Frame = -3 Query: 344 SVDFGPVSVENECGHCTDALSTRCIGASVYSTL 246 SV+ PVSV+NE H S+R AS TL Sbjct: 138 SVELNPVSVDNEVAHGLAVFSSRDAAASARDTL 170 >SPAC823.03 |ppk15||serine/threonine protein kinase Ppk15 |Schizosaccharomyces pombe|chr 1|||Manual Length = 534 Score = 28.7 bits (61), Expect = 0.92 Identities = 12/28 (42%), Positives = 16/28 (57%) Frame = -2 Query: 195 NPLARWTPSCCKVHND*LGSSVSHFSLE 112 NPL RWTP K H G+S S + ++ Sbjct: 442 NPLKRWTPDQAKNHPFITGASFSQYCID 469 >SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 1337 Score = 27.1 bits (57), Expect = 2.8 Identities = 13/26 (50%), Positives = 15/26 (57%) Frame = +3 Query: 432 VAGHMDLITFITKSECECLNEADDHP 509 V H+D I FITKS C + E D P Sbjct: 1285 VVNHIDRIMFITKSLCGEIFEIDKWP 1310 >SPBC13G1.10c |mug81||ATP-dependent RNA helicase Slh1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1935 Score = 26.6 bits (56), Expect = 3.7 Identities = 13/25 (52%), Positives = 18/25 (72%) Frame = +2 Query: 170 LGVHRAKGLPLFSSSYQQSFREQYF 244 LGV+R KGL FSS+++ EQ+F Sbjct: 471 LGVNRYKGLFYFSSAFRPCPIEQHF 495 >SPCC1259.10 |pgp1||metallopeptidase Pgp1|Schizosaccharomyces pombe|chr 3|||Manual Length = 412 Score = 26.6 bits (56), Expect = 3.7 Identities = 13/53 (24%), Positives = 24/53 (45%) Frame = -3 Query: 344 SVDFGPVSVENECGHCTDALSTRCIGASVYSTLRNTALGNFAGSCSKKGAILW 186 S+DF +++ GH S + + T + A+G++ C+K I W Sbjct: 170 SIDFPYLNILVSGGHTMLVYSNSLLNHEIIVTTSDIAVGDYLDKCAKYLGIPW 222 >SPAC4F10.16c |||P-type ATPase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1367 Score = 26.6 bits (56), Expect = 3.7 Identities = 10/17 (58%), Positives = 13/17 (76%) Frame = -1 Query: 655 LYRFGSFVSWTLIFIEC 605 +++FG FVSWT IEC Sbjct: 1196 VFKFGDFVSWTGRNIEC 1212 >SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces pombe|chr 2|||Manual Length = 726 Score = 26.2 bits (55), Expect = 4.9 Identities = 13/51 (25%), Positives = 24/51 (47%) Frame = +3 Query: 213 ATSKVSESSISQSRVDRCADTASAQGVSAMPTFIFYRNRTKIDRLEGGNTT 365 A +V S+ VD + +S + A+PT Y+N ++ + G +T Sbjct: 71 AKEQVEGSNFHFGEVDCSKELSSCANIRAVPTLYLYQNGEIVEEVPFGAST 121 >SPAC167.05 ||SPAC57A7.01|Usp |Schizosaccharomyces pombe|chr 1|||Manual Length = 601 Score = 26.2 bits (55), Expect = 4.9 Identities = 10/30 (33%), Positives = 18/30 (60%) Frame = +3 Query: 357 NTTVLENKVRQYYGSDVAEEDEDNTVAGHM 446 NT+ + + + +YGSD+ E ++D A M Sbjct: 366 NTSFVSSNINNWYGSDLEELEQDVKTAKSM 395 >SPBC2A9.09 |||phosducin family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 233 Score = 26.2 bits (55), Expect = 4.9 Identities = 13/48 (27%), Positives = 25/48 (52%), Gaps = 1/48 (2%) Frame = +1 Query: 112 FQTEMANAGTKL-VVVDFTATWCPPCQRIAPFFEQLPAKFPRAVFLKV 252 + E+ +A ++ VVV P C+ +A E+L +P+ F+K+ Sbjct: 97 YTAEVTDASKEVFVVVHMFQDSLPACKLLAAILERLAPMYPQIKFVKI 144 >SPAC19G12.07c |rsd1||RNA-binding protein Rsd1|Schizosaccharomyces pombe|chr 1|||Manual Length = 604 Score = 25.8 bits (54), Expect = 6.5 Identities = 12/23 (52%), Positives = 13/23 (56%) Frame = +2 Query: 239 YFSK*SRQMRRYSECSGRQCNAH 307 Y S+ SR RRY E S R AH Sbjct: 180 YSSRHSRSRRRYEERSSRSSRAH 202 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,811,039 Number of Sequences: 5004 Number of extensions: 54324 Number of successful extensions: 167 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 151 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 165 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 351258950 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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