BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00306
(549 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC29A4.15 |||serine-tRNA ligase|Schizosaccharomyces pombe|chr ... 30 0.26
SPCC1827.08c |pof7|SPCC70.11c|F-box protein Pof7|Schizosaccharom... 27 1.4
SPAC4G8.05 |ppk14||serine/threonine protein kinase Ppk14 |Schizo... 27 2.4
SPBC25B2.04c |||mitochondrial ribosome assembly protein|Schizosa... 26 4.2
SPBC1703.12 |ubp9||ubiquitin C-terminal hydrolase Ubp9|Schizosac... 25 7.3
SPAPB1E7.06c |eme1||Holliday junction resolvase subunit Eme1|Sch... 25 9.7
SPBC646.08c |||oxysterol binding protein |Schizosaccharomyces po... 25 9.7
SPAC7D4.06c |alg3||dolichol-P-Man dependent alpha|Schizosaccharo... 25 9.7
>SPAC29A4.15 |||serine-tRNA ligase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 450
Score = 29.9 bits (64), Expect = 0.26
Identities = 15/55 (27%), Positives = 28/55 (50%), Gaps = 5/55 (9%)
Frame = +3
Query: 96 IPILSDDEPNAITLKWAPRRTTEER----LYEDIRSNVTG*DQQNGYALH-HQSY 245
+P+ D++ N I KWAP T E+ + ++ + + G D + G + H+ Y
Sbjct: 116 VPVSMDEDNNEIIRKWAPEGVTVEKKNCLSHHEVLTRLDGYDPERGVKVSGHRGY 170
>SPCC1827.08c |pof7|SPCC70.11c|F-box protein
Pof7|Schizosaccharomyces pombe|chr 3|||Manual
Length = 361
Score = 27.5 bits (58), Expect = 1.4
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = +1
Query: 400 KRLEDVTALENDKVEFYVTFSGIPSPTIAWFKDDYENLV 516
KR E+ L +D E +T S +PSPT+ + E++V
Sbjct: 71 KRNEEEEMLNSDASEAMLTVSSVPSPTLTENESVNESVV 109
>SPAC4G8.05 |ppk14||serine/threonine protein kinase Ppk14
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 566
Score = 26.6 bits (56), Expect = 2.4
Identities = 13/34 (38%), Positives = 15/34 (44%)
Frame = +3
Query: 39 KNVHWKSAGRPAPPGKPQIIPILSDDEPNAITLK 140
KNV W PP P++ PI PN LK
Sbjct: 486 KNVQWALLRHTEPPIIPKLAPIDEKGNPNISHLK 519
>SPBC25B2.04c |||mitochondrial ribosome assembly
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 328
Score = 25.8 bits (54), Expect = 4.2
Identities = 15/53 (28%), Positives = 28/53 (52%), Gaps = 1/53 (1%)
Frame = +1
Query: 367 RSSVAGT-SGFIKRLEDVTALENDKVEFYVTFSGIPSPTIAWFKDDYENLVVG 522
+S++ G G KR+ ++ L ND + + GI +P+I +D + +VG
Sbjct: 174 KSAIVGNYPGVTKRISEIVRLFNDMDVYMLDTPGIMTPSITKPEDMLKLSLVG 226
>SPBC1703.12 |ubp9||ubiquitin C-terminal hydrolase
Ubp9|Schizosaccharomyces pombe|chr 2|||Manual
Length = 585
Score = 25.0 bits (52), Expect = 7.3
Identities = 12/35 (34%), Positives = 15/35 (42%)
Frame = +2
Query: 119 AECNYIKMGATTHDGGAPLRGYQVECNRLGSTEWI 223
AE Y H GG P RG+ V R + W+
Sbjct: 354 AERMYYLSSVIVHVGGGPHRGHYVSIVRTKTYGWV 388
>SPAPB1E7.06c |eme1||Holliday junction resolvase subunit
Eme1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 738
Score = 24.6 bits (51), Expect = 9.7
Identities = 10/21 (47%), Positives = 16/21 (76%)
Frame = -2
Query: 440 TLSFSKAVTSSKRLIKPEVPA 378
T SF+ A+TSS ++KP +P+
Sbjct: 225 TKSFNDALTSSSSILKPCMPS 245
>SPBC646.08c |||oxysterol binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 516
Score = 24.6 bits (51), Expect = 9.7
Identities = 16/40 (40%), Positives = 20/40 (50%), Gaps = 2/40 (5%)
Frame = +3
Query: 3 ARAASHNAFKPRKNVHWKSAGRP--APPGKPQIIPILSDD 116
AR SH +KPR V + GRP GK + LSD+
Sbjct: 472 AREESHTEWKPRFFVPDEKEGRPTLTEEGKKVLEQSLSDE 511
>SPAC7D4.06c |alg3||dolichol-P-Man dependent
alpha|Schizosaccharomyces pombe|chr 1|||Manual
Length = 406
Score = 24.6 bits (51), Expect = 9.7
Identities = 10/28 (35%), Positives = 14/28 (50%), Gaps = 3/28 (10%)
Frame = -2
Query: 104 YWYYLWFTWWCGSSC---RFPMNVLPGL 30
Y +Y WF W+ C FP ++ GL
Sbjct: 339 YQFYAWFAWYSPYLCYQASFPAPIVIGL 366
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,084,966
Number of Sequences: 5004
Number of extensions: 37654
Number of successful extensions: 109
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 100
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 109
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 227943826
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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