BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00304 (555 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC14F5.05c |sam1||S-adenosylmethionine synthetase |Schizosacch... 110 1e-25 SPCC11E10.03 |mug1||dynactin complex subunit |Schizosaccharomyce... 26 4.3 SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 7.5 SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange facto... 25 9.9 SPAC694.02 |||DEAD/DEAH box helicase|Schizosaccharomyces pombe|c... 25 9.9 SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces pombe... 25 9.9 SPBC23E6.04c |utp10||U3 snoRNP-associated protein Utp10 |Schizos... 25 9.9 >SPBC14F5.05c |sam1||S-adenosylmethionine synthetase |Schizosaccharomyces pombe|chr 2|||Manual Length = 382 Score = 110 bits (265), Expect = 1e-25 Identities = 49/81 (60%), Positives = 65/81 (80%) Frame = +3 Query: 12 SVMLALDQQSPNIAAGVHENRNDEEVGAGDQGLMFGYATDETEECMPLTVVLAHKLNQKI 191 +V++A++QQSP+IA G+H + EE+GAGDQG+MFGYATDET E +PLT++LAHKLN + Sbjct: 92 NVLVAIEQQSPDIAQGLHYEKALEELGAGDQGIMFGYATDETPEKLPLTILLAHKLNAAM 151 Query: 192 EELRRNGEFWWARPDSKTQVT 254 RR+G W RPD+KTQVT Sbjct: 152 SVARRDGSLPWLRPDTKTQVT 172 Score = 96.3 bits (229), Expect = 3e-21 Identities = 43/77 (55%), Positives = 54/77 (70%) Frame = +2 Query: 257 EYVFAGGATVPQRVHTVVVSLQHSEKITLETLRDEIREKVIKEVIPAQYLDERTVIHINP 436 EY GA +P+RV T+VVS QH++ I+ E LR EI EK+IK +PA LDE+TV HI P Sbjct: 174 EYEEENGAVIPRRVDTIVVSAQHADSISTEDLRSEILEKIIKPTVPAHLLDEKTVYHIQP 233 Query: 437 CGLFIIGGPQSDAGPDG 487 G F++GGPQ DAG G Sbjct: 234 SGRFVVGGPQGDAGLTG 250 Score = 30.7 bits (66), Expect = 0.15 Identities = 14/20 (70%), Positives = 14/20 (70%) Frame = +3 Query: 477 GLTGRKIIVDTMAGGGRTAG 536 GLTGRKIIVDT G G G Sbjct: 247 GLTGRKIIVDTYGGWGAHGG 266 >SPCC11E10.03 |mug1||dynactin complex subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 351 Score = 25.8 bits (54), Expect = 4.3 Identities = 13/40 (32%), Positives = 26/40 (65%) Frame = +3 Query: 150 PLTVVLAHKLNQKIEELRRNGEFWWARPDSKTQVTANMYL 269 PL+ +L +K +K+E + ++ +FW +R +S Q+ A +L Sbjct: 183 PLSELL-NKCMEKLEIVEQDPQFWQSRIESWKQLLAKDFL 221 >SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 534 Score = 25.0 bits (52), Expect = 7.5 Identities = 16/51 (31%), Positives = 25/51 (49%) Frame = -3 Query: 163 TTVNGMHSSVSSVA*PNIKPWSPAPTSSSFLFSCTPAAMFGDCWSSASITL 11 ++ + SVSS + +P TSSS+L S + + SS+S TL Sbjct: 180 SSTSSSQPSVSSTSSSTFSSAAPTSTSSSYLSSSSVVSSSSSPSSSSSSTL 230 >SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange factor Sec74|Schizosaccharomyces pombe|chr 1|||Manual Length = 928 Score = 24.6 bits (51), Expect = 9.9 Identities = 21/68 (30%), Positives = 30/68 (44%) Frame = +3 Query: 132 ETEECMPLTVVLAHKLNQKIEELRRNGEFWWARPDSKTQVTANMYLLVVQQSHREFIL*S 311 E EC + V + K+EE R GE ++ + + AN+ L QS L Sbjct: 726 EVLECSKVRVYIVQNRIYKLEEALRQGE---SKMTVQRRNAANILHLEPIQSRTRIRLAR 782 Query: 312 CHCNTLKR 335 C NTLK+ Sbjct: 783 C-SNTLKK 789 >SPAC694.02 |||DEAD/DEAH box helicase|Schizosaccharomyces pombe|chr 1|||Manual Length = 1717 Score = 24.6 bits (51), Expect = 9.9 Identities = 16/45 (35%), Positives = 21/45 (46%), Gaps = 2/45 (4%) Frame = +3 Query: 132 ETEECMPLTVVLAHKLNQKIEELRRNGEFW--WARPDSKTQVTAN 260 + +EC L ++ L +K E R N E W R K Q TAN Sbjct: 1099 DRQECEYLAEIVFKTLVEKEREWRENSEEWKEKMREYKKWQSTAN 1143 >SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 496 Score = 24.6 bits (51), Expect = 9.9 Identities = 11/28 (39%), Positives = 16/28 (57%) Frame = -1 Query: 474 ASD*GPPIIKSPHGLM*ITVLSSRYCAG 391 A D G P++ + G + T+ RYCAG Sbjct: 103 AMDSGKPLVSNARGDVDGTIALLRYCAG 130 >SPBC23E6.04c |utp10||U3 snoRNP-associated protein Utp10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1649 Score = 24.6 bits (51), Expect = 9.9 Identities = 12/25 (48%), Positives = 16/25 (64%) Frame = -2 Query: 218 KFSISPELFNFLIEFVCKHYGQRHA 144 +FS SPELF IE V YG +++ Sbjct: 1209 RFSASPELFISPIEVVSGPYGLKNS 1233 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,335,034 Number of Sequences: 5004 Number of extensions: 47830 Number of successful extensions: 137 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 134 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 137 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 231978230 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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