BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00303 (643 letters) Database: bee 438 sequences; 146,343 total letters Searching......................................................done Score E Sequences producing significant alignments: (bits) Value AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 178 4e-47 AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 176 1e-46 X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 174 5e-46 EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 164 6e-43 AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 33 0.002 AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.1 DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450 monoo... 23 2.5 AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 2.5 D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 3.3 AB204559-1|BAD89804.1| 832|Apis mellifera soluble guanylyl cycl... 23 3.3 AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase pro... 21 7.7 >AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha F2 protein. Length = 461 Score = 178 bits (433), Expect = 4e-47 Identities = 89/116 (76%), Positives = 97/116 (83%) Frame = +1 Query: 160 QTYHREVREGGPGNG*RILQICLGIGQTKG*AERGITIDIALWKFETSKYYVTIIDAPGH 339 + + +E +E G G+ + + + K ERGITIDIALWKFETSKYYVTIIDAPGH Sbjct: 40 EKFEKEAQEMGKGS----FKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGH 95 Query: 340 RDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVG 507 RDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVG Sbjct: 96 RDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVG 151 Score = 141 bits (342), Expect = 5e-36 Identities = 65/66 (98%), Positives = 65/66 (98%) Frame = +2 Query: 56 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 235 MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60 Query: 236 DKLKAE 253 DKLKAE Sbjct: 61 DKLKAE 66 Score = 98.3 bits (234), Expect = 6e-23 Identities = 44/45 (97%), Positives = 44/45 (97%) Frame = +2 Query: 509 NKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWH 643 NKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWH Sbjct: 153 NKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWH 197 >AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-alpha protein. Length = 274 Score = 176 bits (429), Expect = 1e-46 Identities = 85/92 (92%), Positives = 87/92 (94%) Frame = +1 Query: 232 IGQTKG*AERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG 411 + + K ERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG Sbjct: 3 LDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG 62 Query: 412 TGEFEAGISKNGQTREHALLAFTLGVKQLIVG 507 TGEFEAGISKNGQTREHALLAFTLGVKQLIVG Sbjct: 63 TGEFEAGISKNGQTREHALLAFTLGVKQLIVG 94 Score = 98.3 bits (234), Expect = 6e-23 Identities = 44/45 (97%), Positives = 44/45 (97%) Frame = +2 Query: 509 NKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWH 643 NKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWH Sbjct: 96 NKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWH 140 Score = 23.4 bits (48), Expect = 1.9 Identities = 9/9 (100%), Positives = 9/9 (100%) Frame = +2 Query: 227 WVLDKLKAE 253 WVLDKLKAE Sbjct: 1 WVLDKLKAE 9 >X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha protein. Length = 461 Score = 174 bits (424), Expect = 5e-46 Identities = 87/116 (75%), Positives = 96/116 (82%) Frame = +1 Query: 160 QTYHREVREGGPGNG*RILQICLGIGQTKG*AERGITIDIALWKFETSKYYVTIIDAPGH 339 + + +E +E G G+ + + + K ERGITIDIALWKFET+KYYVTIIDAPGH Sbjct: 40 EKFEKEAQEMGKGS----FKYAWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGH 95 Query: 340 RDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVG 507 RDFIKNMITGTSQADCAVLIVAAG GEFEAGISKNGQTREHALLAFTLGVKQLIVG Sbjct: 96 RDFIKNMITGTSQADCAVLIVAAGIGEFEAGISKNGQTREHALLAFTLGVKQLIVG 151 Score = 141 bits (342), Expect = 5e-36 Identities = 65/66 (98%), Positives = 65/66 (98%) Frame = +2 Query: 56 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 235 MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60 Query: 236 DKLKAE 253 DKLKAE Sbjct: 61 DKLKAE 66 Score = 90.6 bits (215), Expect = 1e-20 Identities = 40/45 (88%), Positives = 42/45 (93%) Frame = +2 Query: 509 NKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWH 643 NKMD T+PPYSE RFEEIKKEVSSYIKKIGYN A+VAFVPISGWH Sbjct: 153 NKMDMTDPPYSEARFEEIKKEVSSYIKKIGYNTASVAFVPISGWH 197 >EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-alpha protein. Length = 172 Score = 164 bits (399), Expect = 6e-43 Identities = 78/78 (100%), Positives = 78/78 (100%) Frame = +1 Query: 274 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 453 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60 Query: 454 REHALLAFTLGVKQLIVG 507 REHALLAFTLGVKQLIVG Sbjct: 61 REHALLAFTLGVKQLIVG 78 Score = 98.3 bits (234), Expect = 6e-23 Identities = 44/45 (97%), Positives = 44/45 (97%) Frame = +2 Query: 509 NKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWH 643 NKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWH Sbjct: 80 NKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWH 124 >AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation factor 2 protein. Length = 690 Score = 33.1 bits (72), Expect = 0.002 Identities = 16/35 (45%), Positives = 18/35 (51%) Frame = +1 Query: 313 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 417 VT +D PGH FI G D VL+VAA G Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDG 229 Score = 25.4 bits (53), Expect = 0.47 Identities = 9/18 (50%), Positives = 13/18 (72%) Frame = +2 Query: 68 KTHINIVVIGHVDSGKST 121 K H + ++GHVD GK+T Sbjct: 143 KRHPIVTIMGHVDHGKTT 160 >AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methyltransferase protein. Length = 683 Score = 24.2 bits (50), Expect = 1.1 Identities = 11/15 (73%), Positives = 11/15 (73%) Frame = +2 Query: 77 INIVVIGHVDSGKST 121 INI IGHV GKST Sbjct: 43 INIGTIGHVAHGKST 57 >DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450 monooxygenase protein. Length = 517 Score = 23.0 bits (47), Expect = 2.5 Identities = 10/17 (58%), Positives = 12/17 (70%) Frame = -2 Query: 69 FSLPIFG*SRITNCV*Y 19 FSLPIFG I +C+ Y Sbjct: 57 FSLPIFGTRWIFSCIGY 73 >AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase protein. Length = 567 Score = 23.0 bits (47), Expect = 2.5 Identities = 13/34 (38%), Positives = 18/34 (52%) Frame = +1 Query: 298 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 399 T KYY D P + FIKN+ ++ +D LI Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327 >D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. Length = 567 Score = 22.6 bits (46), Expect = 3.3 Identities = 12/34 (35%), Positives = 18/34 (52%) Frame = +1 Query: 298 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 399 T KYY D P + FIKN+ ++ +D L+ Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327 >AB204559-1|BAD89804.1| 832|Apis mellifera soluble guanylyl cyclase beta-3 protein. Length = 832 Score = 22.6 bits (46), Expect = 3.3 Identities = 9/24 (37%), Positives = 14/24 (58%) Frame = +2 Query: 506 ENKMDSTEPPYSEPRFEEIKKEVS 577 EN + Y E R+EEI+++ S Sbjct: 7 ENMSEYIRQVYGEDRWEEIRRQAS 30 >AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase protein. Length = 580 Score = 21.4 bits (43), Expect = 7.7 Identities = 7/20 (35%), Positives = 13/20 (65%) Frame = +3 Query: 516 WIPLNHHTVSPDLRKSRRKY 575 W+P+N + S +L +R+Y Sbjct: 443 WLPVNENYKSLNLAAQKREY 462 Database: bee Posted date: Oct 23, 2007 1:17 PM Number of letters in database: 146,343 Number of sequences in database: 438 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 182,852 Number of Sequences: 438 Number of extensions: 3633 Number of successful extensions: 27 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 19 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 27 length of database: 146,343 effective HSP length: 55 effective length of database: 122,253 effective search space used: 19315974 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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