Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00303
(643 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 156 1e-38
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 156 1e-38
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 156 1e-38
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 156 1e-38
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 95 4e-20
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 93 2e-19
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 70 1e-12
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 68 6e-12
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 38 0.004
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 38 0.004
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 36 0.030
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 36 0.030
At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 35 0.040
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 35 0.040
At4g18330.2 68417.m02719 eukaryotic translation initiation facto... 33 0.12
At4g18330.1 68417.m02718 eukaryotic translation initiation facto... 33 0.12
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 33 0.16
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 33 0.16
At1g04170.1 68414.m00407 eukaryotic translation initiation facto... 33 0.16
At4g11160.1 68417.m01808 translation initiation factor IF-2, mit... 33 0.21
At2g18720.1 68415.m02180 eukaryotic translation initiation facto... 33 0.21
At5g13650.2 68418.m01585 elongation factor family protein contai... 30 1.5
At5g13650.1 68418.m01584 elongation factor family protein contai... 30 1.5
At1g76825.1 68414.m08940 eukaryotic translation initiation facto... 30 1.5
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 30 1.5
At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 30 1.5
At2g28000.1 68415.m03393 RuBisCO subunit binding-protein alpha s... 29 2.0
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 29 2.0
At1g21160.1 68414.m02646 eukaryotic translation initiation facto... 29 2.0
At1g49290.1 68414.m05525 expressed protein ; expression supporte... 29 3.5
At4g17215.1 68417.m02589 expressed protein 28 4.6
At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein s... 28 4.6
At4g24015.1 68417.m03451 zinc finger (RING-H2 type) protein-rela... 28 6.1
At1g17220.1 68414.m02098 translation initiation factor IF-2, chl... 28 6.1
At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329... 27 8.0
At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329... 27 8.0
At5g11610.2 68418.m01356 exostosin family protein contains Pfam ... 27 8.0
At5g11610.1 68418.m01355 exostosin family protein contains Pfam ... 27 8.0
At1g78850.1 68414.m09191 curculin-like (mannose-binding) lectin ... 27 8.0
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 156 bits (378), Expect = 1e-38
Identities = 73/90 (81%), Positives = 81/90 (90%)
Frame = +1
Query: 232 IGQTKG*AERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG 411
+ + K ERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ +
Sbjct: 60 LDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDST 119
Query: 412 TGEFEAGISKNGQTREHALLAFTLGVKQLI 501
TG FEAGISK+GQTREHALLAFTLGVKQ+I
Sbjct: 120 TGGFEAGISKDGQTREHALLAFTLGVKQMI 149
Score = 126 bits (303), Expect = 2e-29
Identities = 59/66 (89%), Positives = 60/66 (90%)
Frame = +2
Query: 56 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 235
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 236 DKLKAE 253
DKLKAE
Sbjct: 61 DKLKAE 66
Score = 68.9 bits (161), Expect = 3e-12
Identities = 29/44 (65%), Positives = 37/44 (84%)
Frame = +2
Query: 509 NKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGW 640
NKMD+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+
Sbjct: 153 NKMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGF 196
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 156 bits (378), Expect = 1e-38
Identities = 73/90 (81%), Positives = 81/90 (90%)
Frame = +1
Query: 232 IGQTKG*AERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG 411
+ + K ERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ +
Sbjct: 60 LDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDST 119
Query: 412 TGEFEAGISKNGQTREHALLAFTLGVKQLI 501
TG FEAGISK+GQTREHALLAFTLGVKQ+I
Sbjct: 120 TGGFEAGISKDGQTREHALLAFTLGVKQMI 149
Score = 126 bits (303), Expect = 2e-29
Identities = 59/66 (89%), Positives = 60/66 (90%)
Frame = +2
Query: 56 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 235
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 236 DKLKAE 253
DKLKAE
Sbjct: 61 DKLKAE 66
Score = 68.9 bits (161), Expect = 3e-12
Identities = 29/44 (65%), Positives = 37/44 (84%)
Frame = +2
Query: 509 NKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGW 640
NKMD+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+
Sbjct: 153 NKMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGF 196
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 156 bits (378), Expect = 1e-38
Identities = 73/90 (81%), Positives = 81/90 (90%)
Frame = +1
Query: 232 IGQTKG*AERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG 411
+ + K ERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ +
Sbjct: 60 LDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDST 119
Query: 412 TGEFEAGISKNGQTREHALLAFTLGVKQLI 501
TG FEAGISK+GQTREHALLAFTLGVKQ+I
Sbjct: 120 TGGFEAGISKDGQTREHALLAFTLGVKQMI 149
Score = 126 bits (303), Expect = 2e-29
Identities = 59/66 (89%), Positives = 60/66 (90%)
Frame = +2
Query: 56 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 235
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 236 DKLKAE 253
DKLKAE
Sbjct: 61 DKLKAE 66
Score = 68.9 bits (161), Expect = 3e-12
Identities = 29/44 (65%), Positives = 37/44 (84%)
Frame = +2
Query: 509 NKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGW 640
NKMD+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+
Sbjct: 153 NKMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGF 196
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 156 bits (378), Expect = 1e-38
Identities = 73/90 (81%), Positives = 81/90 (90%)
Frame = +1
Query: 232 IGQTKG*AERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG 411
+ + K ERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ +
Sbjct: 60 LDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDST 119
Query: 412 TGEFEAGISKNGQTREHALLAFTLGVKQLI 501
TG FEAGISK+GQTREHALLAFTLGVKQ+I
Sbjct: 120 TGGFEAGISKDGQTREHALLAFTLGVKQMI 149
Score = 126 bits (303), Expect = 2e-29
Identities = 59/66 (89%), Positives = 60/66 (90%)
Frame = +2
Query: 56 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 235
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 236 DKLKAE 253
DKLKAE
Sbjct: 61 DKLKAE 66
Score = 68.9 bits (161), Expect = 3e-12
Identities = 29/44 (65%), Positives = 37/44 (84%)
Frame = +2
Query: 509 NKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGW 640
NKMD+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+
Sbjct: 153 NKMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGF 196
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 95.1 bits (226), Expect = 4e-20
Identities = 43/82 (52%), Positives = 57/82 (69%)
Frame = +1
Query: 259 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 438
+G T+++ FET TI+DAPGH+ ++ NMI+G SQAD VL+++A GEFE G
Sbjct: 163 KGKTVEVGRAHFETESTRFTILDAPGHKSYVPNMISGASQADIGVLVISARKGEFETGYE 222
Query: 439 KNGQTREHALLAFTLGVKQLIV 504
+ GQTREH LA TLGV +LIV
Sbjct: 223 RGGQTREHVQLAKTLGVSKLIV 244
Score = 66.1 bits (154), Expect = 2e-11
Identities = 27/63 (42%), Positives = 45/63 (71%)
Frame = +2
Query: 65 EKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKL 244
+K H+N+V IGHVD+GKST G +++ G +D R I+K+EKEA++ + S+ A+++D
Sbjct: 98 KKRHLNVVFIGHVDAGKSTIGGQILFLSGQVDDRQIQKYEKEAKDKSRESWYMAYIMDTN 157
Query: 245 KAE 253
+ E
Sbjct: 158 EEE 160
Score = 38.7 bits (86), Expect = 0.003
Identities = 18/44 (40%), Positives = 29/44 (65%), Gaps = 1/44 (2%)
Frame = +2
Query: 509 NKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAA-VAFVPISG 637
NKMD +S+ R++EI++++ ++K GYN V F+PISG
Sbjct: 247 NKMDDPTVNWSKERYDEIEQKMVPFLKASGYNTKKDVVFLPISG 290
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 92.7 bits (220), Expect = 2e-19
Identities = 41/84 (48%), Positives = 60/84 (71%), Gaps = 1/84 (1%)
Frame = +1
Query: 256 ERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGI 435
ERGIT+ +A+ F + +++V ++D+PGH+DF+ NMI G +QAD A+L++ A G FEAG
Sbjct: 300 ERGITMTVAVAYFNSKRHHVVLLDSPGHKDFVPNMIAGATQADAAILVIDASVGAFEAGF 359
Query: 436 SK-NGQTREHALLAFTLGVKQLIV 504
GQTREHA + GV+Q+IV
Sbjct: 360 DNLKGQTREHARVLRGFGVEQVIV 383
Score = 73.3 bits (172), Expect = 1e-13
Identities = 30/61 (49%), Positives = 44/61 (72%)
Frame = +2
Query: 71 THINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKA 250
+ +N+ ++GHVDSGKST +G L++ G I ++ + K+EKEA+ GKGSF YAW LD+
Sbjct: 238 SQLNLAIVGHVDSGKSTLSGRLLHLLGRISQKQMHKYEKEAKLQGKGSFAYAWALDESAE 297
Query: 251 E 253
E
Sbjct: 298 E 298
Score = 31.9 bits (69), Expect = 0.37
Identities = 14/42 (33%), Positives = 27/42 (64%)
Frame = +2
Query: 509 NKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 634
NKMD YS+ RF+ IK+ V S+++ + +++ ++P+S
Sbjct: 386 NKMDIVG--YSKERFDLIKQHVGSFLQSCRFKDSSLTWIPLS 425
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 70.1 bits (164), Expect = 1e-12
Identities = 35/82 (42%), Positives = 50/82 (60%)
Frame = +1
Query: 259 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 438
RGITI+ A ++ET + +D PGH D++KNMITG +Q D A+L+V+ G
Sbjct: 126 RGITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMP---- 181
Query: 439 KNGQTREHALLAFTLGVKQLIV 504
QT+EH LLA +GV ++V
Sbjct: 182 ---QTKEHILLAKQVGVPDMVV 200
Score = 39.1 bits (87), Expect = 0.002
Identities = 17/45 (37%), Positives = 25/45 (55%)
Frame = +2
Query: 53 KMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEK 187
K ++K H+NI IGHVD GK+T T L I +K+++
Sbjct: 72 KFERKKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKYDE 116
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 67.7 bits (158), Expect = 6e-12
Identities = 35/82 (42%), Positives = 50/82 (60%)
Frame = +1
Query: 256 ERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGI 435
+RGITI A ++ET+K + +D PGH D++KNMITG +Q D +L+V+ G
Sbjct: 113 KRGITIATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGPMP--- 169
Query: 436 SKNGQTREHALLAFTLGVKQLI 501
QT+EH LLA +GV L+
Sbjct: 170 ----QTKEHILLARQVGVPSLV 187
Score = 32.7 bits (71), Expect = 0.21
Identities = 12/22 (54%), Positives = 16/22 (72%)
Frame = +2
Query: 62 KEKTHINIVVIGHVDSGKSTTT 127
+ K H+N+ IGHVD GK+T T
Sbjct: 63 RNKPHVNVGTIGHVDHGKTTLT 84
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 38.3 bits (85), Expect = 0.004
Identities = 23/69 (33%), Positives = 32/69 (46%)
Frame = +1
Query: 256 ERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGI 435
E+GITI A Y V IID PGH DF + D A+L++ + G I
Sbjct: 116 EKGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSI 175
Query: 436 SKNGQTREH 462
+ + Q R +
Sbjct: 176 TVDRQMRRY 184
Score = 27.9 bits (59), Expect = 6.1
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +2
Query: 80 NIVVIGHVDSGKSTTTGHLIYKCGGI 157
NI + H+DSGK+T T +++ G I
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRI 92
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 38.3 bits (85), Expect = 0.004
Identities = 23/69 (33%), Positives = 32/69 (46%)
Frame = +1
Query: 256 ERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGI 435
E+GITI A Y V IID PGH DF + D A+L++ + G I
Sbjct: 116 EKGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSI 175
Query: 436 SKNGQTREH 462
+ + Q R +
Sbjct: 176 TVDRQMRRY 184
Score = 27.9 bits (59), Expect = 6.1
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +2
Query: 80 NIVVIGHVDSGKSTTTGHLIYKCGGI 157
NI + H+DSGK+T T +++ G I
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRI 92
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 35.5 bits (78), Expect = 0.030
Identities = 18/53 (33%), Positives = 25/53 (47%)
Frame = +1
Query: 259 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 417
+ + + + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 193 KAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 35.5 bits (78), Expect = 0.030
Identities = 18/53 (33%), Positives = 25/53 (47%)
Frame = +1
Query: 259 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 417
+ + + + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 193 KAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At5g08650.1 68418.m01029 GTP-binding protein LepA, putative
Length = 681
Score = 35.1 bits (77), Expect = 0.040
Identities = 19/58 (32%), Positives = 31/58 (53%), Gaps = 4/58 (6%)
Frame = +1
Query: 256 ERGITIDIAL----WKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 417
ERGITI + + +E + + + +ID PGH DF + + + A+L+V A G
Sbjct: 131 ERGITIKLQAARMRYVYEDTPFCLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQG 188
Score = 28.3 bits (60), Expect = 4.6
Identities = 11/33 (33%), Positives = 18/33 (54%)
Frame = +2
Query: 80 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEK 178
N +I H+D GKST L+ G + R +++
Sbjct: 88 NFSIIAHIDHGKSTLADKLLQVTGTVQNRDMKE 120
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 35.1 bits (77), Expect = 0.040
Identities = 17/53 (32%), Positives = 28/53 (52%)
Frame = +1
Query: 259 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 417
R IT+ + + Y + +ID+PGH DF + T +D A+++V A G
Sbjct: 58 RAITMKSSSISLKYKDYSLNLIDSPGHMDFCSEVSTAARLSDGALVLVDAVEG 110
Score = 32.7 bits (71), Expect = 0.21
Identities = 13/25 (52%), Positives = 16/25 (64%)
Frame = +2
Query: 80 NIVVIGHVDSGKSTTTGHLIYKCGG 154
NI ++ HVD GK+T HLI GG
Sbjct: 11 NICILAHVDHGKTTLADHLIASSGG 35
>At4g18330.2 68417.m02719 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 471
Score = 33.5 bits (73), Expect = 0.12
Identities = 19/69 (27%), Positives = 33/69 (47%)
Frame = +1
Query: 298 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAF 477
T + +V+ +D PGH + M+ G + D A+L++AA QT EH
Sbjct: 124 TLRRHVSCVDCPGHDILMATMLNGAAIVDGALLLIAANES------CPQPQTAEHLASVD 177
Query: 478 TLGVKQLIV 504
+ +K +I+
Sbjct: 178 MMRLKHIII 186
>At4g18330.1 68417.m02718 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 284
Score = 33.5 bits (73), Expect = 0.12
Identities = 19/69 (27%), Positives = 33/69 (47%)
Frame = +1
Query: 298 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAF 477
T + +V+ +D PGH + M+ G + D A+L++AA QT EH
Sbjct: 124 TLRRHVSCVDCPGHDILMATMLNGAAIVDGALLLIAANES------CPQPQTAEHLASVD 177
Query: 478 TLGVKQLIV 504
+ +K +I+
Sbjct: 178 MMRLKHIII 186
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 33.1 bits (72), Expect = 0.16
Identities = 16/41 (39%), Positives = 21/41 (51%)
Frame = +1
Query: 295 ETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 417
E S Y + +ID PGH DF + S A+L+V A G
Sbjct: 131 EASGYLLNLIDTPGHVDFSYEVSRSLSACQGALLVVDAAQG 171
Score = 27.9 bits (59), Expect = 6.1
Identities = 16/40 (40%), Positives = 19/40 (47%)
Frame = +2
Query: 44 D*PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK 163
D K EK N +I H+D GKST L+ G I K
Sbjct: 57 DLTKFPSEKIR-NFSIIAHIDHGKSTLADRLMELTGTIKK 95
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 33.1 bits (72), Expect = 0.16
Identities = 19/54 (35%), Positives = 25/54 (46%)
Frame = +1
Query: 256 ERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 417
ERGITI A K+ + IID PGH DF + D A+ + + G
Sbjct: 144 ERGITITSAATTTFWDKHRINIIDTPGHVDFTLEVERALRVLDGAICLFDSVAG 197
Score = 32.7 bits (71), Expect = 0.21
Identities = 12/28 (42%), Positives = 20/28 (71%)
Frame = +2
Query: 68 KTHINIVVIGHVDSGKSTTTGHLIYKCG 151
K + NI ++ H+D+GK+TTT ++Y G
Sbjct: 94 KDYRNIGIMAHIDAGKTTTTERILYYTG 121
>At1g04170.1 68414.m00407 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to gb|U37354 from S. pombe. ESTs
gb|T41979, gb|N37284 and gb|N37529 come from this gene
Length = 465
Score = 33.1 bits (72), Expect = 0.16
Identities = 22/75 (29%), Positives = 35/75 (46%), Gaps = 4/75 (5%)
Frame = +1
Query: 292 FETSKY----YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTRE 459
FE SK +V+ +D PGH + M+ G + D A+L++AA QT E
Sbjct: 112 FENSKMKLLRHVSFVDCPGHDILMATMLNGAAIMDGALLLIAANE------TCPQPQTSE 165
Query: 460 HALLAFTLGVKQLIV 504
H + +K +I+
Sbjct: 166 HLAAVEIMQLKHIII 180
>At4g11160.1 68417.m01808 translation initiation factor IF-2,
mitochondrial, putative similar to SP|P46198|IF2M_BOVIN
Translation initiation factor IF-2, mitochondrial
precursor (IF-2Mt) (IF-2(Mt)) {Bos taurus}
Length = 743
Score = 32.7 bits (71), Expect = 0.21
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +1
Query: 313 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 417
+T +D PGH F + G + D VL+VAA G
Sbjct: 270 ITFLDTPGHAAFSEMRARGAAVTDIVVLVVAADDG 304
>At2g18720.1 68415.m02180 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative
Length = 465
Score = 32.7 bits (71), Expect = 0.21
Identities = 19/65 (29%), Positives = 31/65 (47%)
Frame = +1
Query: 310 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGV 489
+V+ +D PGH + M+ G + D A+LI+AA QT EH + +
Sbjct: 120 HVSFVDCPGHDILMATMLNGAAIMDGALLIIAANE------TCPQPQTAEHLASVDMMHL 173
Query: 490 KQLIV 504
K +I+
Sbjct: 174 KDIII 178
>At5g13650.2 68418.m01585 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 676
Score = 29.9 bits (64), Expect = 1.5
Identities = 19/54 (35%), Positives = 24/54 (44%)
Frame = +1
Query: 256 ERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 417
ERGITI V IID PGH DF + + D +L+V + G
Sbjct: 129 ERGITILSKNTSITYKNTKVNIIDTPGHSDFGGEVERVLNMVDGVLLVVDSVEG 182
>At5g13650.1 68418.m01584 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 675
Score = 29.9 bits (64), Expect = 1.5
Identities = 19/54 (35%), Positives = 24/54 (44%)
Frame = +1
Query: 256 ERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 417
ERGITI V IID PGH DF + + D +L+V + G
Sbjct: 128 ERGITILSKNTSITYKNTKVNIIDTPGHSDFGGEVERVLNMVDGVLLVVDSVEG 181
>At1g76825.1 68414.m08940 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profile PF00009: Elongation
factor Tu GTP binding domain
Length = 630
Score = 29.9 bits (64), Expect = 1.5
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +1
Query: 319 IIDAPGHRDFIKNMITGTSQADCAVLIV 402
+ID PGH F G+S D A+L+V
Sbjct: 113 VIDTPGHESFTNLRSRGSSLCDLAILVV 140
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 29.9 bits (64), Expect = 1.5
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +1
Query: 319 IIDAPGHRDFIKNMITGTSQADCAVLIV 402
+ID PGH F G+S D A+L+V
Sbjct: 772 VIDTPGHESFTNLRSRGSSLCDLAILVV 799
>At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1201
Score = 29.9 bits (64), Expect = 1.5
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +1
Query: 319 IIDAPGHRDFIKNMITGTSQADCAVLIV 402
+ID PGH F G+S D A+L+V
Sbjct: 705 VIDTPGHESFTNLRSRGSSLCDLAILVV 732
>At2g28000.1 68415.m03393 RuBisCO subunit binding-protein alpha
subunit, chloroplast / 60 kDa chaperonin alpha subunit /
CPN-60 alpha identical to SWISS-PROT:P21238- RuBisCO
subunit binding-protein alpha subunit, chloroplast
precursor (60 kDa chaperonin alpha subunit, CPN-60
alpha) [Arabidopsis thaliana]
Length = 586
Score = 29.5 bits (63), Expect = 2.0
Identities = 15/58 (25%), Positives = 30/58 (51%)
Frame = -2
Query: 405 SYDEHSAISLRGSCDHVLDEISVSRSINDGNIVLASFELPESNIDCDTTLSSAFSLSN 232
++D+HS +L+ D + D + ++ N+VL F P+ ++ T++ A L N
Sbjct: 52 AFDQHSRAALQAGIDKLADCVGLTLGPRGRNVVLDEFGSPKV-VNDGVTIARAIELPN 108
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 29.5 bits (63), Expect = 2.0
Identities = 11/34 (32%), Positives = 20/34 (58%)
Frame = +1
Query: 301 SKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 402
++Y + +ID+PGH DF + D A+++V
Sbjct: 96 NEYLINLIDSPGHVDFSSEVTAALRITDGALVVV 129
Score = 29.1 bits (62), Expect = 2.6
Identities = 13/24 (54%), Positives = 15/24 (62%)
Frame = +2
Query: 80 NIVVIGHVDSGKSTTTGHLIYKCG 151
N+ VI HVD GKST T L+ G
Sbjct: 21 NMSVIAHVDHGKSTLTDSLVAAAG 44
>At1g21160.1 68414.m02646 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1088
Score = 29.5 bits (63), Expect = 2.0
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = +1
Query: 313 VTIIDAPGHRDFIKNMITGTSQADCAVLIV 402
+ +ID PGH F G++ D A+L+V
Sbjct: 558 ILVIDTPGHESFTNLRSRGSNLCDLAILVV 587
>At1g49290.1 68414.m05525 expressed protein ; expression supported
by MPSS
Length = 338
Score = 28.7 bits (61), Expect = 3.5
Identities = 19/58 (32%), Positives = 25/58 (43%), Gaps = 2/58 (3%)
Frame = +3
Query: 462 CLARFHPRCQTAHR-RRTKWIPLNHHTVSPDLRKSRRK-YPHTSRRLATTQLLSLSCP 629
C F C+TAHR R K + L D++K R S RL+ Q+ S P
Sbjct: 149 CTRAFSDLCETAHRINREKQLALEREKFIEDMKKLRLSLQQEKSNRLSVQQVKIASPP 206
>At4g17215.1 68417.m02589 expressed protein
Length = 150
Score = 28.3 bits (60), Expect = 4.6
Identities = 9/13 (69%), Positives = 11/13 (84%)
Frame = +3
Query: 483 RCQTAHRRRTKWI 521
+C T HRRR+KWI
Sbjct: 65 KCHTGHRRRSKWI 77
>At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein
similar to 1,4-alpha-glucan branching enzyme [Solanum
tuberosum] GI:1621012, 1,4-alpha-glucan branching enzyme
(EC 2.4.1.18) from [Homo sapiens] SP|Q04446, {Solanum
tuberosum} SP|P30924; contains Pfam profiles: PF00128
Alpha amylase catalytic domain, PF02922 Isoamylase
N-terminal domain
Length = 777
Score = 28.3 bits (60), Expect = 4.6
Identities = 14/33 (42%), Positives = 20/33 (60%), Gaps = 3/33 (9%)
Frame = +2
Query: 539 SEPR---FEEIKKEVSSYIKKIGYNPAAVAFVP 628
SEP+ FEE K+V ++K+ GYN + VP
Sbjct: 257 SEPKVSTFEEFTKKVLPHVKRAGYNAIQLIGVP 289
>At4g24015.1 68417.m03451 zinc finger (RING-H2 type) protein-related
low similarity to RING-H2 zinc finger protein ATL4
[Arabidopsis thaliana] GI:4928399
Length = 174
Score = 27.9 bits (59), Expect = 6.1
Identities = 16/42 (38%), Positives = 23/42 (54%)
Frame = +2
Query: 221 YAWVLDKLKAELSVVSQSILLSGSSKLASTMLPSLMLLDTEI 346
Y + L + + LS S IL SS S+ LPS+ LLD ++
Sbjct: 42 YLFYLKRRASSLSSPSPMILPVSSSHQTSSHLPSVCLLDVKV 83
>At1g17220.1 68414.m02098 translation initiation factor IF-2,
chloroplast, putative similar to SP|P57997|IF2C_PHAVU
Translation initiation factor IF-2, chloroplast
precursor (PvIF2cp) {Phaseolus vulgaris}
Length = 1026
Score = 27.9 bits (59), Expect = 6.1
Identities = 12/32 (37%), Positives = 16/32 (50%)
Frame = +1
Query: 322 IDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 417
+D PGH F G D A+++VAA G
Sbjct: 557 LDTPGHEAFGAMRARGARVTDIAIIVVAADDG 588
>At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329:
Protein of unknown function (DUF731)
Length = 442
Score = 27.5 bits (58), Expect = 8.0
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +2
Query: 521 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 634
+TE + P+ E K E+S++I IG+ V F P S
Sbjct: 63 ATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCS 100
>At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329:
Protein of unknown function (DUF731)
Length = 440
Score = 27.5 bits (58), Expect = 8.0
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +2
Query: 521 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 634
+TE + P+ E K E+S++I IG+ V F P S
Sbjct: 63 ATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCS 100
>At5g11610.2 68418.m01356 exostosin family protein contains Pfam
domain, PF03016: Exostosin family
Length = 453
Score = 27.5 bits (58), Expect = 8.0
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +3
Query: 492 TAHRRRTKWIPLNHHTVSPDLRKSRRKYPHTS 587
+ RRR+ + H + P + + R+KYPH S
Sbjct: 107 SGQRRRSNETASSLHPLQPKIPQIRKKYPHRS 138
>At5g11610.1 68418.m01355 exostosin family protein contains Pfam
domain, PF03016: Exostosin family
Length = 546
Score = 27.5 bits (58), Expect = 8.0
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +3
Query: 492 TAHRRRTKWIPLNHHTVSPDLRKSRRKYPHTS 587
+ RRR+ + H + P + + R+KYPH S
Sbjct: 107 SGQRRRSNETASSLHPLQPKIPQIRKKYPHRS 138
>At1g78850.1 68414.m09191 curculin-like (mannose-binding) lectin
family protein low similarity to ser/thr protein kinase
from Zea mays [GI:2598067]; contains Pfam lectin
(probable mannose binding) domain PF01453
Length = 441
Score = 27.5 bits (58), Expect = 8.0
Identities = 18/68 (26%), Positives = 32/68 (47%), Gaps = 2/68 (2%)
Frame = +2
Query: 128 GHLIYKCGGIDKRT--IEKFEKEAQEMGKGSFKYAWVLDKLKAELSVVSQSILLSGSSKL 301
G L+++ +K I+ E + S K+ W + +V QS+ L+G +KL
Sbjct: 120 GRLVWQTNTANKGAVGIKILENGNMVIYDSSGKFVWQSFDSPTDTLLVGQSLKLNGRTKL 179
Query: 302 ASTMLPSL 325
S + PS+
Sbjct: 180 VSRLSPSV 187
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,227,923
Number of Sequences: 28952
Number of extensions: 293144
Number of successful extensions: 991
Number of sequences better than 10.0: 39
Number of HSP's better than 10.0 without gapping: 940
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 988
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1324661040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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