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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00300
         (751 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g42890.1 68418.m05227 sterol carrier protein 2 (SCP-2) family...    41   8e-04
At3g51550.1 68416.m05645 protein kinase family protein contains ...    31   1.1  
At1g67160.1 68414.m07640 F-box family protein similar to F-box p...    29   2.5  
At3g47290.1 68416.m05139 phosphoinositide-specific phospholipase...    29   4.4  
At1g80240.1 68414.m09390 expressed protein contains Pfam profile...    29   4.4  
At1g62220.1 68414.m07018 expressed protein                             28   5.8  

>At5g42890.1 68418.m05227 sterol carrier protein 2 (SCP-2) family
           protein similar to sterol carrier protein-2 [Homo
           sapiens] GI:432973; contains Pfam profile PF02036: SCP-2
           sterol transfer family
          Length = 123

 Score = 41.1 bits (92), Expect = 8e-04
 Identities = 16/36 (44%), Positives = 25/36 (69%)
 Frame = +1

Query: 514 DEDVADLISGKLNPQKAFFQGKIKIQGNMGLAMKLT 621
           D+D   + +GK+NPQ AF +G +KI+G++  A K T
Sbjct: 78  DDDFVKVATGKMNPQMAFIRGAMKIKGSLSAAQKFT 113


>At3g51550.1 68416.m05645 protein kinase family protein contains
           protein kinase domain, Pfam:PF00069
          Length = 895

 Score = 30.7 bits (66), Expect = 1.1
 Identities = 23/65 (35%), Positives = 34/65 (52%), Gaps = 2/65 (3%)
 Frame = -2

Query: 441 MTQ*PSAPSGPFLTLKP*IPRTFSIKLSWSVCMASSRIF--MYLKTLKPSGLSATAATLF 268
           +TQ PS P  P++T      R F    +++  +AS R F  +Y       GL+AT  +LF
Sbjct: 77  LTQDPSVPEVPYMT-----ARVFRSPFTYTFPVASGRKFVRLYFYPNSYDGLNAT-NSLF 130

Query: 267 GVTFG 253
            V+FG
Sbjct: 131 SVSFG 135


>At1g67160.1 68414.m07640 F-box family protein similar to F-box
           protein family, AtFBX7 (GI:20197899) [Arabidopsis
           thaliana]
          Length = 450

 Score = 29.5 bits (63), Expect = 2.5
 Identities = 11/21 (52%), Positives = 15/21 (71%)
 Frame = -3

Query: 626 KSVSFIASPMLPWILIFPWKN 564
           KSV  + +P  PWI++FP KN
Sbjct: 49  KSVIGVTNPTTPWIILFPNKN 69


>At3g47290.1 68416.m05139 phosphoinositide-specific phospholipase C
           family protein similar to phosphoinositide-specific
           phospholipase C [Nicotiana rustica] GI:1771381,
           1-phosphatidylinositol-4,5-bisphosphate
           phosphodiesterase [Nicotiana rustica] GI:2765140;
           contains Pfam profiles PF00168: C2 domain, PF00388:
           Phosphatidylinositol-specific phospholipase C, X domain
          Length = 531

 Score = 28.7 bits (61), Expect = 4.4
 Identities = 19/73 (26%), Positives = 30/73 (41%), Gaps = 3/73 (4%)
 Frame = -2

Query: 228 RQHPRDPSYVAALSGRVA-PHGRRQASRNSESELIR--WWYSXXXXXXXXXXXXISRDNN 58
           RQHP DP+Y + +S  V  P G  Q     ++  I+   WY              +R   
Sbjct: 271 RQHPADPNYQSLVSFHVVEPRGMLQNVLTGKANKIQRPGWYETDIISFTQKRFLRTRPQR 330

Query: 57  KYLRMSTERPSKS 19
           K L  +  +P ++
Sbjct: 331 KLLIYAPYKPQRA 343


>At1g80240.1 68414.m09390 expressed protein contains Pfam profile
           PF04862: Protein of unknown function, DUF642
          Length = 370

 Score = 28.7 bits (61), Expect = 4.4
 Identities = 18/65 (27%), Positives = 30/65 (46%)
 Frame = +2

Query: 296 PEGFKVFKYMKILEEAMQTDQDNLIEKVRGIYGFKVRNGPDGAEGYWVINAKEGKGKVTY 475
           PEG +  + +   E A+       + K   +  F V +  DG EG  ++ A  G+GKV  
Sbjct: 251 PEGHRAIELVGGKESAISQIVRTSLNKFYALT-FNVGDARDGCEGPMIVEAFAGQGKVMV 309

Query: 476 NGSEK 490
           + + K
Sbjct: 310 DYASK 314


>At1g62220.1 68414.m07018 expressed protein
          Length = 153

 Score = 28.3 bits (60), Expect = 5.8
 Identities = 23/57 (40%), Positives = 30/57 (52%)
 Frame = +3

Query: 297 RKALRSSNT*RSLKRPCKPTRTT*SRKSAGSTVSRSETVQTAPRVTGSSMRKKAKGK 467
           RKA  SS +  + K   K   T   RK+A +  +    V +A RV GSS +KK KGK
Sbjct: 95  RKARVSSASASTAKGDAKAAVT---RKAAAARANGK--VASASRVKGSSEKKKGKGK 146


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,948,801
Number of Sequences: 28952
Number of extensions: 329514
Number of successful extensions: 849
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 822
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 849
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1663169840
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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