Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00297
(686 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 271 2e-73
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 271 2e-73
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 271 2e-73
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 271 2e-73
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 159 2e-39
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 157 8e-39
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 87 1e-17
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 79 4e-15
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 45 4e-05
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 45 4e-05
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 45 4e-05
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 43 2e-04
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 41 7e-04
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 41 7e-04
At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 41 9e-04
At4g18330.2 68417.m02719 eukaryotic translation initiation facto... 37 0.014
At4g18330.1 68417.m02718 eukaryotic translation initiation facto... 37 0.014
At1g04170.1 68414.m00407 eukaryotic translation initiation facto... 36 0.025
At2g18720.1 68415.m02180 eukaryotic translation initiation facto... 35 0.044
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 34 0.10
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 34 0.10
At4g11160.1 68417.m01808 translation initiation factor IF-2, mit... 33 0.23
At1g76825.1 68414.m08940 eukaryotic translation initiation facto... 30 1.7
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 30 1.7
At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 30 1.7
At1g21160.1 68414.m02646 eukaryotic translation initiation facto... 29 2.2
At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329... 29 3.8
At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329... 29 3.8
At3g43240.1 68416.m04564 ARID/BRIGHT DNA-binding domain-containi... 28 5.0
At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein s... 28 5.0
At5g64600.1 68418.m08118 expressed protein similar to axi 1 [Nic... 28 6.7
At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5) M... 28 6.7
At4g20200.1 68417.m02953 terpene synthase/cyclase family protein... 28 6.7
At1g76530.1 68414.m08906 auxin efflux carrier family protein con... 28 6.7
At1g17220.1 68414.m02098 translation initiation factor IF-2, chl... 28 6.7
At2g02410.1 68415.m00181 expressed protein 27 8.8
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 271 bits (665), Expect = 2e-73
Identities = 131/155 (84%), Positives = 139/155 (89%), Gaps = 1/155 (0%)
Frame = +2
Query: 47 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 226
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 227 DKLKAERD-WFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 403
DKLKAER+ TIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 404 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKM 508
G FEAGISK+GQTREHALLAFTLGVKQ+I NKM
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKM 155
Score = 87.8 bits (208), Expect = 6e-18
Identities = 36/58 (62%), Positives = 47/58 (81%)
Frame = +1
Query: 508 DSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 681
D+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+KG
Sbjct: 156 DATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 271 bits (665), Expect = 2e-73
Identities = 131/155 (84%), Positives = 139/155 (89%), Gaps = 1/155 (0%)
Frame = +2
Query: 47 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 226
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 227 DKLKAERD-WFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 403
DKLKAER+ TIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 404 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKM 508
G FEAGISK+GQTREHALLAFTLGVKQ+I NKM
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKM 155
Score = 87.8 bits (208), Expect = 6e-18
Identities = 36/58 (62%), Positives = 47/58 (81%)
Frame = +1
Query: 508 DSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 681
D+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+KG
Sbjct: 156 DATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 271 bits (665), Expect = 2e-73
Identities = 131/155 (84%), Positives = 139/155 (89%), Gaps = 1/155 (0%)
Frame = +2
Query: 47 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 226
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 227 DKLKAERD-WFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 403
DKLKAER+ TIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 404 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKM 508
G FEAGISK+GQTREHALLAFTLGVKQ+I NKM
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKM 155
Score = 87.8 bits (208), Expect = 6e-18
Identities = 36/58 (62%), Positives = 47/58 (81%)
Frame = +1
Query: 508 DSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 681
D+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+KG
Sbjct: 156 DATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 271 bits (665), Expect = 2e-73
Identities = 131/155 (84%), Positives = 139/155 (89%), Gaps = 1/155 (0%)
Frame = +2
Query: 47 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 226
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 227 DKLKAERD-WFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 403
DKLKAER+ TIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 404 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKM 508
G FEAGISK+GQTREHALLAFTLGVKQ+I NKM
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKM 155
Score = 87.8 bits (208), Expect = 6e-18
Identities = 36/58 (62%), Positives = 47/58 (81%)
Frame = +1
Query: 508 DSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 681
D+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+KG
Sbjct: 156 DATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 159 bits (385), Expect = 2e-39
Identities = 71/151 (47%), Positives = 106/151 (70%), Gaps = 2/151 (1%)
Frame = +2
Query: 62 THINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKA 241
+ +N+ ++GHVDSGKST +G L++ G I ++ + K+EKEA+ GKGSF YAW LD+
Sbjct: 238 SQLNLAIVGHVDSGKSTLSGRLLHLLGRISQKQMHKYEKEAKLQGKGSFAYAWALDESAE 297
Query: 242 ERD-WFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEA 418
ER+ T+ +A+ F + +++V ++D+PGH+DF+ NMI G +QAD A+L++ A G FEA
Sbjct: 298 ERERGITMTVAVAYFNSKRHHVVLLDSPGHKDFVPNMIAGATQADAAILVIDASVGAFEA 357
Query: 419 GISK-NGQTREHALLAFTLGVKQLIVGVNKM 508
G GQTREHA + GV+Q+IV +NKM
Sbjct: 358 GFDNLKGQTREHARVLRGFGVEQVIVAINKM 388
Score = 34.7 bits (76), Expect = 0.058
Identities = 15/55 (27%), Positives = 32/55 (58%), Gaps = 3/55 (5%)
Frame = +1
Query: 526 YSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNML-EPSTK--MPWFKG 681
YS+ RF+ IK+ V S+++ + +++ ++P+S N++ PS W++G
Sbjct: 393 YSKERFDLIKQHVGSFLQSCRFKDSSLTWIPLSAMENQNLVAAPSDNRLSSWYQG 447
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 157 bits (380), Expect = 8e-39
Identities = 74/152 (48%), Positives = 105/152 (69%), Gaps = 1/152 (0%)
Frame = +2
Query: 56 EKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKL 235
+K H+N+V IGHVD+GKST G +++ G +D R I+K+EKEA++ + S+ A+++D
Sbjct: 98 KKRHLNVVFIGHVDAGKSTIGGQILFLSGQVDDRQIQKYEKEAKDKSRESWYMAYIMDTN 157
Query: 236 KAER-DWFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEF 412
+ ER T+++ FET TI+DAPGH+ ++ NMI+G SQAD VL+++A GEF
Sbjct: 158 EEERLKGKTVEVGRAHFETESTRFTILDAPGHKSYVPNMISGASQADIGVLVISARKGEF 217
Query: 413 EAGISKNGQTREHALLAFTLGVKQLIVGVNKM 508
E G + GQTREH LA TLGV +LIV VNKM
Sbjct: 218 ETGYERGGQTREHVQLAKTLGVSKLIVVVNKM 249
Score = 39.9 bits (89), Expect = 0.002
Identities = 23/65 (35%), Positives = 39/65 (60%), Gaps = 3/65 (4%)
Frame = +1
Query: 496 SKQNDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAA-VAFVPISGWHGDNMLEPSTK--M 666
+K +D T +S+ R++EI++++ ++K GYN V F+PISG G NM + +
Sbjct: 247 NKMDDPTVN-WSKERYDEIEQKMVPFLKASGYNTKKDVVFLPISGLMGKNMDQRMGQEIC 305
Query: 667 PWFKG 681
PW+ G
Sbjct: 306 PWWSG 310
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 86.6 bits (205), Expect = 1e-17
Identities = 54/155 (34%), Positives = 79/155 (50%), Gaps = 1/155 (0%)
Frame = +2
Query: 44 KMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWV 223
K ++K H+NI IGHVD GK+T T L I +K+++
Sbjct: 72 KFERKKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKYDE--------------- 116
Query: 224 LDKLKAERD-WFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG 400
+D ER TI+ A ++ET + +D PGH D++KNMITG +Q D A+L+V+
Sbjct: 117 IDAAPEERARGITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 176
Query: 401 TGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK 505
G QT+EH LLA +GV ++V +NK
Sbjct: 177 DGPMP-------QTKEHILLAKQVGVPDMVVFLNK 204
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 78.6 bits (185), Expect = 4e-15
Identities = 53/153 (34%), Positives = 76/153 (49%), Gaps = 1/153 (0%)
Frame = +2
Query: 53 KEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK 232
+ K H+N+ IGHVD GK+T T + K E GK +DK
Sbjct: 63 RNKPHVNVGTIGHVDHGKTTLTAAIT---------------KVLAEEGKAKAIAFDEIDK 107
Query: 233 LKAERD-WFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGE 409
E+ TI A ++ET+K + +D PGH D++KNMITG +Q D +L+V+ G
Sbjct: 108 APEEKKRGITIATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGP 167
Query: 410 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKM 508
QT+EH LLA +GV L+ +NK+
Sbjct: 168 MP-------QTKEHILLARQVGVPSLVCFLNKV 193
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 45.2 bits (102), Expect = 4e-05
Identities = 32/112 (28%), Positives = 50/112 (44%)
Frame = +2
Query: 71 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERD 250
NI ++ HVD GK+T HLI GG G ++ LD+ +R
Sbjct: 11 NICILAHVDHGKTTLADHLIASSGG----------GVLHPRLAGKLRFMDYLDE--EQRR 58
Query: 251 WFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 406
T+ + + Y + +ID+PGH DF + T +D A+++V A G
Sbjct: 59 AITMKSSSISLKYKDYSLNLIDSPGHMDFCSEVSTAARLSDGALVLVDAVEG 110
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 45.2 bits (102), Expect = 4e-05
Identities = 38/128 (29%), Positives = 58/128 (45%), Gaps = 1/128 (0%)
Frame = +2
Query: 71 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERD 250
NI + H+DSGK+T T +++ G I E E ++ G G+ +D + ER+
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRIH----EIHEVRGRD-GVGA-----KMDSMDLERE 116
Query: 251 -WFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 427
TI A Y V IID PGH DF + D A+L++ + G I+
Sbjct: 117 KGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSIT 176
Query: 428 KNGQTREH 451
+ Q R +
Sbjct: 177 VDRQMRRY 184
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 45.2 bits (102), Expect = 4e-05
Identities = 38/128 (29%), Positives = 58/128 (45%), Gaps = 1/128 (0%)
Frame = +2
Query: 71 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERD 250
NI + H+DSGK+T T +++ G I E E ++ G G+ +D + ER+
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRIH----EIHEVRGRD-GVGA-----KMDSMDLERE 116
Query: 251 -WFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 427
TI A Y V IID PGH DF + D A+L++ + G I+
Sbjct: 117 KGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSIT 176
Query: 428 KNGQTREH 451
+ Q R +
Sbjct: 177 VDRQMRRY 184
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 42.7 bits (96), Expect = 2e-04
Identities = 37/124 (29%), Positives = 53/124 (42%)
Frame = +2
Query: 35 D*PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKY 214
D K EK N +I H+D GKST L+ G TI+K + Q + K +
Sbjct: 57 DLTKFPSEKIR-NFSIIAHIDHGKSTLADRLMELTG-----TIKKGHGQPQYLDKLQRER 110
Query: 215 AWVLDKLKAERDWFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 394
+ KA+ + + E S Y + +ID PGH DF + S A+L+V
Sbjct: 111 GITV---KAQTATMFYENKVEDQEASGYLLNLIDTPGHVDFSYEVSRSLSACQGALLVVD 167
Query: 395 AGTG 406
A G
Sbjct: 168 AAQG 171
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 41.1 bits (92), Expect = 7e-04
Identities = 32/116 (27%), Positives = 53/116 (45%)
Frame = +2
Query: 59 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 238
K + NI ++ H+D+GK+TTT ++Y G + E+ +G+ W+ + +
Sbjct: 94 KDYRNIGIMAHIDAGKTTTTERILYYTGR---------NYKIGEVHEGTATMDWM--EQE 142
Query: 239 AERDWFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 406
ER TI A K+ + IID PGH DF + D A+ + + G
Sbjct: 143 QERG-ITITSAATTTFWDKHRINIIDTPGHVDFTLEVERALRVLDGAICLFDSVAG 197
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 41.1 bits (92), Expect = 7e-04
Identities = 31/111 (27%), Positives = 51/111 (45%), Gaps = 4/111 (3%)
Frame = +2
Query: 71 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRT---IEKFEKEAQEMGKG-SFKYAWVLDKLK 238
N+ VI HVD GKST T L+ G I + + + A E +G + K + L
Sbjct: 21 NMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTRADEAERGITIKSTGI--SLY 78
Query: 239 AERDWFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 391
E ++ + ++Y + +ID+PGH DF + D A+++V
Sbjct: 79 YEMTDESLKSFTGARDGNEYLINLIDSPGHVDFSSEVTAALRITDGALVVV 129
>At5g08650.1 68418.m01029 GTP-binding protein LepA, putative
Length = 681
Score = 40.7 bits (91), Expect = 9e-04
Identities = 28/112 (25%), Positives = 52/112 (46%)
Frame = +2
Query: 71 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERD 250
N +I H+D GKST L+ G + R +++ + ++ + + KL+A R
Sbjct: 88 NFSIIAHIDHGKSTLADKLLQVTGTVQNRDMKEQFLDNMDLERER----GITIKLQAARM 143
Query: 251 WFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 406
+ +E + + + +ID PGH DF + + + A+L+V A G
Sbjct: 144 RYV-------YEDTPFCLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQG 188
>At4g18330.2 68417.m02719 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 471
Score = 36.7 bits (81), Expect = 0.014
Identities = 22/78 (28%), Positives = 38/78 (48%)
Frame = +2
Query: 287 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAF 466
T + +V+ +D PGH + M+ G + D A+L++AA QT EH
Sbjct: 124 TLRRHVSCVDCPGHDILMATMLNGAAIVDGALLLIAANES------CPQPQTAEHLASVD 177
Query: 467 TLGVKQLIVGVNKMIPLN 520
+ +K +I+ NK+ +N
Sbjct: 178 MMRLKHIIILQNKIDLIN 195
>At4g18330.1 68417.m02718 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 284
Score = 36.7 bits (81), Expect = 0.014
Identities = 22/78 (28%), Positives = 38/78 (48%)
Frame = +2
Query: 287 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAF 466
T + +V+ +D PGH + M+ G + D A+L++AA QT EH
Sbjct: 124 TLRRHVSCVDCPGHDILMATMLNGAAIVDGALLLIAANES------CPQPQTAEHLASVD 177
Query: 467 TLGVKQLIVGVNKMIPLN 520
+ +K +I+ NK+ +N
Sbjct: 178 MMRLKHIIILQNKIDLIN 195
>At1g04170.1 68414.m00407 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to gb|U37354 from S. pombe. ESTs
gb|T41979, gb|N37284 and gb|N37529 come from this gene
Length = 465
Score = 35.9 bits (79), Expect = 0.025
Identities = 28/99 (28%), Positives = 47/99 (47%), Gaps = 4/99 (4%)
Frame = +2
Query: 281 FETSKY----YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTRE 448
FE SK +V+ +D PGH + M+ G + D A+L++AA QT E
Sbjct: 112 FENSKMKLLRHVSFVDCPGHDILMATMLNGAAIMDGALLLIAANE------TCPQPQTSE 165
Query: 449 HALLAFTLGVKQLIVGVNKMIPLNHHTVSPDLRKSRRKY 565
H + +K +I+ NK I L V+ + ++ +K+
Sbjct: 166 HLAAVEIMQLKHIIILQNK-IDLIQENVAINQHEAIQKF 203
>At2g18720.1 68415.m02180 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative
Length = 465
Score = 35.1 bits (77), Expect = 0.044
Identities = 21/70 (30%), Positives = 34/70 (48%)
Frame = +2
Query: 299 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGV 478
+V+ +D PGH + M+ G + D A+LI+AA QT EH + +
Sbjct: 120 HVSFVDCPGHDILMATMLNGAAIMDGALLIIAANE------TCPQPQTAEHLASVDMMHL 173
Query: 479 KQLIVGVNKM 508
K +I+ NK+
Sbjct: 174 KDIIIIQNKI 183
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 33.9 bits (74), Expect = 0.10
Identities = 18/49 (36%), Positives = 23/49 (46%)
Frame = +2
Query: 260 IDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 406
+ + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 197 MSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 33.9 bits (74), Expect = 0.10
Identities = 18/49 (36%), Positives = 23/49 (46%)
Frame = +2
Query: 260 IDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 406
+ + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 197 MSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At4g11160.1 68417.m01808 translation initiation factor IF-2,
mitochondrial, putative similar to SP|P46198|IF2M_BOVIN
Translation initiation factor IF-2, mitochondrial
precursor (IF-2Mt) (IF-2(Mt)) {Bos taurus}
Length = 743
Score = 32.7 bits (71), Expect = 0.23
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +2
Query: 302 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 406
+T +D PGH F + G + D VL+VAA G
Sbjct: 270 ITFLDTPGHAAFSEMRARGAAVTDIVVLVVAADDG 304
>At1g76825.1 68414.m08940 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profile PF00009: Elongation
factor Tu GTP binding domain
Length = 630
Score = 29.9 bits (64), Expect = 1.7
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 308 IIDAPGHRDFIKNMITGTSQADCAVLIV 391
+ID PGH F G+S D A+L+V
Sbjct: 113 VIDTPGHESFTNLRSRGSSLCDLAILVV 140
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 29.9 bits (64), Expect = 1.7
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 308 IIDAPGHRDFIKNMITGTSQADCAVLIV 391
+ID PGH F G+S D A+L+V
Sbjct: 772 VIDTPGHESFTNLRSRGSSLCDLAILVV 799
>At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1201
Score = 29.9 bits (64), Expect = 1.7
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 308 IIDAPGHRDFIKNMITGTSQADCAVLIV 391
+ID PGH F G+S D A+L+V
Sbjct: 705 VIDTPGHESFTNLRSRGSSLCDLAILVV 732
>At1g21160.1 68414.m02646 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1088
Score = 29.5 bits (63), Expect = 2.2
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = +2
Query: 302 VTIIDAPGHRDFIKNMITGTSQADCAVLIV 391
+ +ID PGH F G++ D A+L+V
Sbjct: 558 ILVIDTPGHESFTNLRSRGSNLCDLAILVV 587
>At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329:
Protein of unknown function (DUF731)
Length = 442
Score = 28.7 bits (61), Expect = 3.8
Identities = 15/40 (37%), Positives = 22/40 (55%)
Frame = +1
Query: 505 NDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 624
N +TE + P+ E K E+S++I IG+ V F P S
Sbjct: 61 NVATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCS 100
>At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329:
Protein of unknown function (DUF731)
Length = 440
Score = 28.7 bits (61), Expect = 3.8
Identities = 15/40 (37%), Positives = 22/40 (55%)
Frame = +1
Query: 505 NDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 624
N +TE + P+ E K E+S++I IG+ V F P S
Sbjct: 61 NVATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCS 100
>At3g43240.1 68416.m04564 ARID/BRIGHT DNA-binding domain-containing
protein contains Pfam profile PF01388: ARID/BRIGHT DNA
binding domain
Length = 747
Score = 28.3 bits (60), Expect = 5.0
Identities = 13/34 (38%), Positives = 16/34 (47%)
Frame = +3
Query: 582 DWLQPSCCRFRAHFWMARRQHVGAFNQNALVQGM 683
DW+ C AHF RR +GAF A G+
Sbjct: 687 DWVNCGSCGEWAHFGCDRRPGLGAFKDYAKTDGL 720
>At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein
similar to 1,4-alpha-glucan branching enzyme [Solanum
tuberosum] GI:1621012, 1,4-alpha-glucan branching enzyme
(EC 2.4.1.18) from [Homo sapiens] SP|Q04446, {Solanum
tuberosum} SP|P30924; contains Pfam profiles: PF00128
Alpha amylase catalytic domain, PF02922 Isoamylase
N-terminal domain
Length = 777
Score = 28.3 bits (60), Expect = 5.0
Identities = 14/33 (42%), Positives = 20/33 (60%), Gaps = 3/33 (9%)
Frame = +1
Query: 529 SEPR---FEEIKKEVSSYIKKIGYNPAAVAFVP 618
SEP+ FEE K+V ++K+ GYN + VP
Sbjct: 257 SEPKVSTFEEFTKKVLPHVKRAGYNAIQLIGVP 289
>At5g64600.1 68418.m08118 expressed protein similar to axi 1
[Nicotiana tabacum] GI:559921; contains Pfam profile
PF03138: Plant protein family
Length = 522
Score = 27.9 bits (59), Expect = 6.7
Identities = 19/68 (27%), Positives = 33/68 (48%), Gaps = 2/68 (2%)
Frame = +1
Query: 472 RCQTAHRRSKQNDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHG--DNM 645
R T+H + K +STE E KEV ++K +GY+ + V ++ +G D +
Sbjct: 313 RESTSHWKIKSINSTEQR-EEGLCPLTPKEVGIFLKGLGYSQSTVIYIAAGEIYGGDDRL 371
Query: 646 LEPSTKMP 669
E ++ P
Sbjct: 372 SELKSRFP 379
>At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5)
Monovalent cation:proton antiporter family 2 (CPA2
family) member, PMID:11500563; related to
glutathione-regulated potassium-efflux system protein
[Escherichia coli] GP|606284|gb|AAA58147
Length = 568
Score = 27.9 bits (59), Expect = 6.7
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = +1
Query: 535 PRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNM 645
PRF ++ ++SS ++ Y AAVAF +S W D +
Sbjct: 354 PRFLKLMIQLSSQTNEL-YQLAAVAFCLLSAWCSDKL 389
>At4g20200.1 68417.m02953 terpene synthase/cyclase family protein
5-epi-aristolochene synthase, Nicotiana tabacum,
PATX:G505588
Length = 604
Score = 27.9 bits (59), Expect = 6.7
Identities = 16/47 (34%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
Frame = +2
Query: 170 FEKEAQEMGKGSFKYAWVLDKLK-AERDWFTIDIALWKFETSKYYVT 307
FE+E E K SF+ +DK+ E D +T+ I W F Y+++
Sbjct: 132 FEEEIYETLKMSFEN---IDKMMDGEDDLYTVSIIFWVFRRHGYHIS 175
>At1g76530.1 68414.m08906 auxin efflux carrier family protein
contains auxin efflux carrier domain, Pfam:PF03547
Length = 415
Score = 27.9 bits (59), Expect = 6.7
Identities = 15/57 (26%), Positives = 28/57 (49%), Gaps = 4/57 (7%)
Frame = +1
Query: 457 ARFHPRCQTAHRRSKQNDSTEPPYSEPRFEEIKKEVSSYIKKIG----YNPAAVAFV 615
+ + C+ SK+ + E + R+EE+K+ V S KK+ + PA +A +
Sbjct: 201 SNYDDSCKVQLISSKEEEKEEDNHQVGRWEEVKQRVVSLSKKVNLGSIFAPATIAAI 257
>At1g17220.1 68414.m02098 translation initiation factor IF-2,
chloroplast, putative similar to SP|P57997|IF2C_PHAVU
Translation initiation factor IF-2, chloroplast
precursor (PvIF2cp) {Phaseolus vulgaris}
Length = 1026
Score = 27.9 bits (59), Expect = 6.7
Identities = 12/32 (37%), Positives = 16/32 (50%)
Frame = +2
Query: 311 IDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 406
+D PGH F G D A+++VAA G
Sbjct: 557 LDTPGHEAFGAMRARGARVTDIAIIVVAADDG 588
>At2g02410.1 68415.m00181 expressed protein
Length = 308
Score = 27.5 bits (58), Expect = 8.8
Identities = 17/47 (36%), Positives = 21/47 (44%)
Frame = +2
Query: 167 KFEKEAQEMGKGSFKYAWVLDKLKAERDWFTIDIALWKFETSKYYVT 307
K KE Q G G K A K K E+D D L++ T+ Y T
Sbjct: 75 KLWKEFQSRGSGMAKPATSYRKKKVEKDELPDDSELYRDPTNTLYYT 121
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,416,100
Number of Sequences: 28952
Number of extensions: 327048
Number of successful extensions: 1080
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 1022
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1065
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1457719448
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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