BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00295 (754 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g13950.1 68414.m01639 eukaryotic translation initiation facto... 105 3e-23 At1g69410.1 68414.m07972 eukaryotic translation initiation facto... 101 4e-22 At1g26630.1 68414.m03243 eukaryotic translation initiation facto... 97 1e-20 At5g47690.1 68418.m05887 expressed protein 31 1.1 At1g56345.1 68414.m06477 pseudouridine synthase family protein l... 29 2.5 At5g03140.1 68418.m00262 lectin protein kinase family protein co... 29 4.4 At4g37250.1 68417.m05273 leucine-rich repeat family protein / pr... 29 4.4 At1g62420.1 68414.m07042 expressed protein contains Pfam profile... 29 4.4 At3g51290.1 68416.m05614 proline-rich family protein 28 5.8 At2g33255.1 68415.m04075 haloacid dehalogenase-like hydrolase fa... 28 7.7 >At1g13950.1 68414.m01639 eukaryotic translation initiation factor 5A-1 / eIF-5A 1 identical to SP|Q9XI91 Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) {Arabidopsis thaliana} Length = 158 Score = 105 bits (252), Expect = 3e-23 Identities = 43/72 (59%), Positives = 56/72 (77%) Frame = +3 Query: 63 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 242 E+ HFE+ D+GAS T+P Q +RKNG++++K RPCK+VE+STSKTGKHGHAK H V ID Sbjct: 4 EEHHFESSDAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63 Query: 243 IFNGKSMKISVP 278 IF K ++ VP Sbjct: 64 IFTSKKLEDIVP 75 Score = 94.7 bits (225), Expect = 6e-20 Identities = 41/75 (54%), Positives = 58/75 (77%), Gaps = 1/75 (1%) Frame = +2 Query: 257 KYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGD-LGTQLRT 433 K EDI PS+HN DVPHV R DYQL DIS+DGY++L+ DNG ++DLK+P+ D L Q+++ Sbjct: 69 KLEDIVPSSHNCDVPHVNRTDYQLIDISEDGYVSLLTDNGSTKDDLKLPNDDTLLQQIKS 128 Query: 434 DFDSGKELLCTVLKS 478 FD GK+L+ +V+ + Sbjct: 129 GFDDGKDLVVSVMSA 143 >At1g69410.1 68414.m07972 eukaryotic translation initiation factor 5A, putative / eIF-5A, putative strong similarity to eukaryotic initiation factor 5A (2) (Nicotiana plumbaginifolia) GI:19702, SP|Q9AXQ6| Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) {Lycopersicon esculentum} Length = 158 Score = 101 bits (243), Expect = 4e-22 Identities = 42/72 (58%), Positives = 55/72 (76%) Frame = +3 Query: 63 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 242 ++ HFE+ D+GAS T+P Q +RK G +++KGRPCK+VE+STSKTGKHGHAK H V ID Sbjct: 4 DEHHFESSDAGASKTYPQQAGNIRKGGHIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAID 63 Query: 243 IFNGKSMKISVP 278 IF K ++ VP Sbjct: 64 IFTSKKLEDIVP 75 Score = 91.5 bits (217), Expect = 5e-19 Identities = 40/75 (53%), Positives = 57/75 (76%), Gaps = 1/75 (1%) Frame = +2 Query: 257 KYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLGTQLRT 433 K EDI PS+HN DVPHV R DYQL DIS+DG+++L+ DNG ++DLK+P D L TQL+ Sbjct: 69 KLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQLKN 128 Query: 434 DFDSGKELLCTVLKS 478 F+ GK+++ +V+ + Sbjct: 129 GFEEGKDIVVSVMSA 143 >At1g26630.1 68414.m03243 eukaryotic translation initiation factor 5A, putative / eIF-5A, putative strong similariy to SP|Q9AXQ6 Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) {Lycopersicon esculentum} Length = 159 Score = 96.7 bits (230), Expect = 1e-20 Identities = 40/72 (55%), Positives = 52/72 (72%) Frame = +3 Query: 63 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 242 ++ HFE +SGAS T+P +RK G +++K RPCK+VE+STSKTGKHGHAK H V ID Sbjct: 4 DEHHFEASESGASKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63 Query: 243 IFNGKSMKISVP 278 IF K ++ VP Sbjct: 64 IFTAKKLEDIVP 75 Score = 90.2 bits (214), Expect = 1e-18 Identities = 39/75 (52%), Positives = 56/75 (74%), Gaps = 1/75 (1%) Frame = +2 Query: 257 KYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLGTQLRT 433 K EDI PS+HN DVPHV R DYQL DI++DG+++L+ D+G ++DLK+P D L Q+R Sbjct: 69 KLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTDDGLTAQMRL 128 Query: 434 DFDSGKELLCTVLKS 478 FD GK+++ +V+ S Sbjct: 129 GFDEGKDIVVSVMSS 143 >At5g47690.1 68418.m05887 expressed protein Length = 1638 Score = 30.7 bits (66), Expect = 1.1 Identities = 23/80 (28%), Positives = 40/80 (50%), Gaps = 6/80 (7%) Frame = -1 Query: 532 LFVESCVCLTAMTHSSPPGFQHGAQQFLAAV---EVSTQLCAEVTIRDFEVLTQVT-VVS 365 L E+ VCL+ + S PP Q FL AV E+ +V + ++++T + + Sbjct: 29 LLKEAAVCLSELEQSPPPAVLKSIQPFLDAVIKPEILNHQDKDVKLLVASCVSEITRITA 88 Query: 364 HQGQVA--IIRDISQLVVFA 311 + + I++DI QL+V A Sbjct: 89 PEAPYSDNIMKDIFQLIVSA 108 >At1g56345.1 68414.m06477 pseudouridine synthase family protein low similarity to SP|P23851 Ribosomal large subunit pseudouridine synthase C (EC 4.2.1.70) (Pseudouridylate synthase) (Uracil hydrolyase) {Escherichia coli}; contains Pfam profile PF00849: RNA pseudouridylate synthase Length = 322 Score = 29.5 bits (63), Expect = 2.5 Identities = 20/69 (28%), Positives = 35/69 (50%), Gaps = 1/69 (1%) Frame = -1 Query: 472 QHGAQQFLAAVEVSTQLCAEVTIRDFEVLTQ-VTVVSHQGQVAIIRDISQLVVFALHVGY 296 +HGA + AA++V L +RD E + V+V S + + + D++ ++V G Sbjct: 176 KHGAWRVYAALDVGRVLPGGSFVRDMETTFEVVSVNSVKNESCELEDVNHVIVAE---GE 232 Query: 295 VHVVCGGTD 269 + CGG D Sbjct: 233 RELSCGGDD 241 >At5g03140.1 68418.m00262 lectin protein kinase family protein contains Pfam domains, PF00138: Legume lectins alpha domain, PF00139: Legume lectins beta domain and PF00069: Protein kinase domain Length = 711 Score = 28.7 bits (61), Expect = 4.4 Identities = 13/36 (36%), Positives = 21/36 (58%) Frame = -1 Query: 163 RPFSITKPFLRRAEHCMGKVAEAPESPVSKCVSSMS 56 +P +T+P +R + A+ PE P++K SSMS Sbjct: 631 QPDPVTRPTMRSVVQILVGEADVPEVPIAKPSSSMS 666 >At4g37250.1 68417.m05273 leucine-rich repeat family protein / protein kinase family protein contains protein kinase domain, Pfam:PF00069; contains leucine-rich repeats, Pfam:PF00560 Length = 768 Score = 28.7 bits (61), Expect = 4.4 Identities = 13/26 (50%), Positives = 16/26 (61%) Frame = +1 Query: 676 PSPSLTSLAIPHPLTSPTRFRSLAPS 753 PSPS +S+ P +P FRSL PS Sbjct: 637 PSPSPSSVGAMSPYCAPESFRSLKPS 662 >At1g62420.1 68414.m07042 expressed protein contains Pfam profile PF04720: Protein of unknown function (DUF506) Length = 465 Score = 28.7 bits (61), Expect = 4.4 Identities = 18/56 (32%), Positives = 29/56 (51%), Gaps = 1/56 (1%) Frame = -1 Query: 520 SCVCLTAMTHSSPPGFQHGAQQFLAAVEVS-TQLCAEVTIRDFEVLTQVTVVSHQG 356 S +C H SPP +QFL+ + V+ L A +++ D EVL ++S+ G Sbjct: 279 SALCRKGTVHPSPPPAIKTDEQFLSLLPVAILSLVAALSVEDREVLAY--LISNSG 332 >At3g51290.1 68416.m05614 proline-rich family protein Length = 602 Score = 28.3 bits (60), Expect = 5.8 Identities = 10/25 (40%), Positives = 14/25 (56%) Frame = +1 Query: 40 KTQQWVTSKTHTLRPETPGPQPPSP 114 +T W T+ T ++ P P P PP P Sbjct: 91 ETTTWTTTTTSSVLPPPPPPPPPPP 115 >At2g33255.1 68415.m04075 haloacid dehalogenase-like hydrolase family protein contains InterPro accession IPR005834: Haloacid dehalogenase-like hydrolase Length = 224 Score = 27.9 bits (59), Expect = 7.7 Identities = 15/40 (37%), Positives = 20/40 (50%) Frame = -3 Query: 344 HQRYQSVGSLRASRGVRPCCVWRDRYLHTFTIEDINPNQV 225 HQR++ + S R RP D LH + DI PN+V Sbjct: 121 HQRFEVIFSPALGREFRPYKPNPDPLLHICSTWDIQPNEV 160 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 17,188,303 Number of Sequences: 28952 Number of extensions: 377215 Number of successful extensions: 1121 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 1036 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1111 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1672953192 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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