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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00295
         (754 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g13950.1 68414.m01639 eukaryotic translation initiation facto...   105   3e-23
At1g69410.1 68414.m07972 eukaryotic translation initiation facto...   101   4e-22
At1g26630.1 68414.m03243 eukaryotic translation initiation facto...    97   1e-20
At5g47690.1 68418.m05887 expressed protein                             31   1.1  
At1g56345.1 68414.m06477 pseudouridine synthase family protein l...    29   2.5  
At5g03140.1 68418.m00262 lectin protein kinase family protein co...    29   4.4  
At4g37250.1 68417.m05273 leucine-rich repeat family protein / pr...    29   4.4  
At1g62420.1 68414.m07042 expressed protein contains Pfam profile...    29   4.4  
At3g51290.1 68416.m05614 proline-rich family protein                   28   5.8  
At2g33255.1 68415.m04075 haloacid dehalogenase-like hydrolase fa...    28   7.7  

>At1g13950.1 68414.m01639 eukaryotic translation initiation factor
           5A-1 / eIF-5A 1 identical to SP|Q9XI91 Eukaryotic
           translation initiation factor 5A-1 (eIF-5A 1)
           {Arabidopsis thaliana}
          Length = 158

 Score =  105 bits (252), Expect = 3e-23
 Identities = 43/72 (59%), Positives = 56/72 (77%)
 Frame = +3

Query: 63  EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 242
           E+ HFE+ D+GAS T+P Q   +RKNG++++K RPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4   EEHHFESSDAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63

Query: 243 IFNGKSMKISVP 278
           IF  K ++  VP
Sbjct: 64  IFTSKKLEDIVP 75



 Score = 94.7 bits (225), Expect = 6e-20
 Identities = 41/75 (54%), Positives = 58/75 (77%), Gaps = 1/75 (1%)
 Frame = +2

Query: 257 KYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGD-LGTQLRT 433
           K EDI PS+HN DVPHV R DYQL DIS+DGY++L+ DNG  ++DLK+P+ D L  Q+++
Sbjct: 69  KLEDIVPSSHNCDVPHVNRTDYQLIDISEDGYVSLLTDNGSTKDDLKLPNDDTLLQQIKS 128

Query: 434 DFDSGKELLCTVLKS 478
            FD GK+L+ +V+ +
Sbjct: 129 GFDDGKDLVVSVMSA 143


>At1g69410.1 68414.m07972 eukaryotic translation initiation factor
           5A, putative / eIF-5A, putative strong similarity to
           eukaryotic initiation factor 5A (2) (Nicotiana
           plumbaginifolia) GI:19702, SP|Q9AXQ6| Eukaryotic
           translation initiation factor 5A-1 (eIF-5A 1)
           {Lycopersicon esculentum}
          Length = 158

 Score =  101 bits (243), Expect = 4e-22
 Identities = 42/72 (58%), Positives = 55/72 (76%)
 Frame = +3

Query: 63  EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 242
           ++ HFE+ D+GAS T+P Q   +RK G +++KGRPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4   DEHHFESSDAGASKTYPQQAGNIRKGGHIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAID 63

Query: 243 IFNGKSMKISVP 278
           IF  K ++  VP
Sbjct: 64  IFTSKKLEDIVP 75



 Score = 91.5 bits (217), Expect = 5e-19
 Identities = 40/75 (53%), Positives = 57/75 (76%), Gaps = 1/75 (1%)
 Frame = +2

Query: 257 KYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLGTQLRT 433
           K EDI PS+HN DVPHV R DYQL DIS+DG+++L+ DNG  ++DLK+P D  L TQL+ 
Sbjct: 69  KLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQLKN 128

Query: 434 DFDSGKELLCTVLKS 478
            F+ GK+++ +V+ +
Sbjct: 129 GFEEGKDIVVSVMSA 143


>At1g26630.1 68414.m03243 eukaryotic translation initiation factor
           5A, putative / eIF-5A, putative strong similariy to
           SP|Q9AXQ6 Eukaryotic translation initiation factor 5A-1
           (eIF-5A 1) {Lycopersicon esculentum}
          Length = 159

 Score = 96.7 bits (230), Expect = 1e-20
 Identities = 40/72 (55%), Positives = 52/72 (72%)
 Frame = +3

Query: 63  EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 242
           ++ HFE  +SGAS T+P     +RK G +++K RPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4   DEHHFEASESGASKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63

Query: 243 IFNGKSMKISVP 278
           IF  K ++  VP
Sbjct: 64  IFTAKKLEDIVP 75



 Score = 90.2 bits (214), Expect = 1e-18
 Identities = 39/75 (52%), Positives = 56/75 (74%), Gaps = 1/75 (1%)
 Frame = +2

Query: 257 KYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLGTQLRT 433
           K EDI PS+HN DVPHV R DYQL DI++DG+++L+ D+G  ++DLK+P D  L  Q+R 
Sbjct: 69  KLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTDDGLTAQMRL 128

Query: 434 DFDSGKELLCTVLKS 478
            FD GK+++ +V+ S
Sbjct: 129 GFDEGKDIVVSVMSS 143


>At5g47690.1 68418.m05887 expressed protein
          Length = 1638

 Score = 30.7 bits (66), Expect = 1.1
 Identities = 23/80 (28%), Positives = 40/80 (50%), Gaps = 6/80 (7%)
 Frame = -1

Query: 532 LFVESCVCLTAMTHSSPPGFQHGAQQFLAAV---EVSTQLCAEVTIRDFEVLTQVT-VVS 365
           L  E+ VCL+ +  S PP      Q FL AV   E+      +V +     ++++T + +
Sbjct: 29  LLKEAAVCLSELEQSPPPAVLKSIQPFLDAVIKPEILNHQDKDVKLLVASCVSEITRITA 88

Query: 364 HQGQVA--IIRDISQLVVFA 311
            +   +  I++DI QL+V A
Sbjct: 89  PEAPYSDNIMKDIFQLIVSA 108


>At1g56345.1 68414.m06477 pseudouridine synthase family protein low
           similarity to SP|P23851 Ribosomal large subunit
           pseudouridine synthase C (EC 4.2.1.70) (Pseudouridylate
           synthase) (Uracil hydrolyase) {Escherichia coli};
           contains Pfam profile PF00849: RNA pseudouridylate
           synthase
          Length = 322

 Score = 29.5 bits (63), Expect = 2.5
 Identities = 20/69 (28%), Positives = 35/69 (50%), Gaps = 1/69 (1%)
 Frame = -1

Query: 472 QHGAQQFLAAVEVSTQLCAEVTIRDFEVLTQ-VTVVSHQGQVAIIRDISQLVVFALHVGY 296
           +HGA +  AA++V   L     +RD E   + V+V S + +   + D++ ++V     G 
Sbjct: 176 KHGAWRVYAALDVGRVLPGGSFVRDMETTFEVVSVNSVKNESCELEDVNHVIVAE---GE 232

Query: 295 VHVVCGGTD 269
             + CGG D
Sbjct: 233 RELSCGGDD 241


>At5g03140.1 68418.m00262 lectin protein kinase family protein
           contains Pfam domains, PF00138: Legume lectins alpha
           domain, PF00139: Legume lectins beta domain and PF00069:
           Protein kinase domain
          Length = 711

 Score = 28.7 bits (61), Expect = 4.4
 Identities = 13/36 (36%), Positives = 21/36 (58%)
 Frame = -1

Query: 163 RPFSITKPFLRRAEHCMGKVAEAPESPVSKCVSSMS 56
           +P  +T+P +R     +   A+ PE P++K  SSMS
Sbjct: 631 QPDPVTRPTMRSVVQILVGEADVPEVPIAKPSSSMS 666


>At4g37250.1 68417.m05273 leucine-rich repeat family protein /
           protein kinase family protein contains protein kinase
           domain, Pfam:PF00069; contains leucine-rich repeats,
           Pfam:PF00560
          Length = 768

 Score = 28.7 bits (61), Expect = 4.4
 Identities = 13/26 (50%), Positives = 16/26 (61%)
 Frame = +1

Query: 676 PSPSLTSLAIPHPLTSPTRFRSLAPS 753
           PSPS +S+    P  +P  FRSL PS
Sbjct: 637 PSPSPSSVGAMSPYCAPESFRSLKPS 662


>At1g62420.1 68414.m07042 expressed protein contains Pfam profile
           PF04720: Protein of unknown function (DUF506)
          Length = 465

 Score = 28.7 bits (61), Expect = 4.4
 Identities = 18/56 (32%), Positives = 29/56 (51%), Gaps = 1/56 (1%)
 Frame = -1

Query: 520 SCVCLTAMTHSSPPGFQHGAQQFLAAVEVS-TQLCAEVTIRDFEVLTQVTVVSHQG 356
           S +C     H SPP      +QFL+ + V+   L A +++ D EVL    ++S+ G
Sbjct: 279 SALCRKGTVHPSPPPAIKTDEQFLSLLPVAILSLVAALSVEDREVLAY--LISNSG 332


>At3g51290.1 68416.m05614 proline-rich family protein 
          Length = 602

 Score = 28.3 bits (60), Expect = 5.8
 Identities = 10/25 (40%), Positives = 14/25 (56%)
 Frame = +1

Query: 40  KTQQWVTSKTHTLRPETPGPQPPSP 114
           +T  W T+ T ++ P  P P PP P
Sbjct: 91  ETTTWTTTTTSSVLPPPPPPPPPPP 115


>At2g33255.1 68415.m04075 haloacid dehalogenase-like hydrolase
           family protein contains InterPro accession IPR005834:
           Haloacid dehalogenase-like hydrolase
          Length = 224

 Score = 27.9 bits (59), Expect = 7.7
 Identities = 15/40 (37%), Positives = 20/40 (50%)
 Frame = -3

Query: 344 HQRYQSVGSLRASRGVRPCCVWRDRYLHTFTIEDINPNQV 225
           HQR++ + S    R  RP     D  LH  +  DI PN+V
Sbjct: 121 HQRFEVIFSPALGREFRPYKPNPDPLLHICSTWDIQPNEV 160


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,188,303
Number of Sequences: 28952
Number of extensions: 377215
Number of successful extensions: 1121
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1036
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1111
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1672953192
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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