Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00294
(499 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_P46776 Cluster: 60S ribosomal protein L27a; n=204; Euka... 89 5e-17
UniRef50_Q9LR33 Cluster: 60S ribosomal protein L27a-2; n=56; Euk... 87 3e-16
UniRef50_Q00V89 Cluster: 60s ribosomal protein L15/L27; n=2; Ost... 79 4e-14
UniRef50_A1BQ56 Cluster: Ribosomal protein L27a; n=4; Bilateria|... 78 1e-13
UniRef50_Q00454 Cluster: 60S ribosomal protein L27a; n=3; Eukary... 78 1e-13
UniRef50_Q9AW02 Cluster: 60S ribosomal protein L27A; n=3; Eukary... 60 4e-08
UniRef50_Q4P992 Cluster: Putative uncharacterized protein; n=1; ... 52 5e-06
UniRef50_O62581 Cluster: 60S ribosomal protein L27a; n=1; Enceph... 49 7e-05
UniRef50_Q3LW97 Cluster: Ribosomal protein L27a; n=1; Bigelowiel... 47 2e-04
UniRef50_A7QSF4 Cluster: Chromosome chr12 scaffold_159, whole ge... 42 0.006
UniRef50_UPI0000DA3FAD Cluster: PREDICTED: similar to 60S riboso... 39 0.072
UniRef50_A4ANL3 Cluster: Putative lipoprotein; n=1; Flavobacteri... 34 2.0
UniRef50_Q9ZQM0 Cluster: Putative non-LTR retroelement reverse t... 33 2.7
UniRef50_Q6AL41 Cluster: Putative uncharacterized protein; n=1; ... 32 6.2
UniRef50_A7RPS0 Cluster: Predicted protein; n=1; Nematostella ve... 32 8.2
>UniRef50_P46776 Cluster: 60S ribosomal protein L27a; n=204;
Eukaryota|Rep: 60S ribosomal protein L27a - Homo sapiens
(Human)
Length = 148
Score = 89.0 bits (211), Expect = 5e-17
Identities = 37/73 (50%), Positives = 48/73 (65%)
Frame = +2
Query: 35 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGCFGKLGMRN 214
M + +KTRKLRGHVS NAGG HHHRIN DKYHPG FGK+GM++
Sbjct: 1 MPSRLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGLHHHRINFDKYHPGYFGKVGMKH 60
Query: 215 FHFRKNKNFCPVL 253
+H ++N++FCP +
Sbjct: 61 YHLKRNQSFCPTV 73
Score = 68.1 bits (159), Expect = 1e-10
Identities = 36/88 (40%), Positives = 46/88 (52%)
Frame = +1
Query: 214 FPL*KEQEFLSSFDLDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPKQ 393
+ L + Q F + +LDKLWTLVSEQTR+ A G P+I++V++ PKQ
Sbjct: 61 YHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGAAPIIDVVRSGYYKVLGKGKLPKQ 120
Query: 394 PVIVXXXXXXXXXXXXXXDVGGACVLSA 477
PVIV VGGACVL A
Sbjct: 121 PVIVKAKFFSRRAEEKIKSVGGACVLVA 148
>UniRef50_Q9LR33 Cluster: 60S ribosomal protein L27a-2; n=56;
Eukaryota|Rep: 60S ribosomal protein L27a-2 -
Arabidopsis thaliana (Mouse-ear cress)
Length = 146
Score = 86.6 bits (205), Expect = 3e-16
Identities = 40/73 (54%), Positives = 45/73 (61%)
Frame = +2
Query: 35 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGCFGKLGMRN 214
MAT+ KK RK RGHVS NAGG HHHRI DKYHPG FGK+GMR
Sbjct: 1 MATALKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 60
Query: 215 FHFRKNKNFCPVL 253
FH +NK FCP++
Sbjct: 61 FHKLRNKFFCPIV 73
Score = 33.1 bits (72), Expect = 3.6
Identities = 24/81 (29%), Positives = 37/81 (45%), Gaps = 1/81 (1%)
Frame = +1
Query: 238 FLSSFDLDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPK-QPVIVXXX 414
F +LDKLW+LV E + K S+ D VP+I++ + P+ +P +V
Sbjct: 69 FCPIVNLDKLWSLVPEDVKAK--SSKD-NVPLIDVTQHGFFKVLGKGHLPENKPFVVKAK 125
Query: 415 XXXXXXXXXXXDVGGACVLSA 477
+ GGA VL+A
Sbjct: 126 LISKTAEKKIKEAGGAVVLTA 146
>UniRef50_Q00V89 Cluster: 60s ribosomal protein L15/L27; n=2;
Ostreococcus|Rep: 60s ribosomal protein L15/L27 -
Ostreococcus tauri
Length = 198
Score = 79.4 bits (187), Expect = 4e-14
Identities = 36/71 (50%), Positives = 41/71 (57%)
Frame = +2
Query: 41 TSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGCFGKLGMRNFH 220
T K RK RGHVS AGG+HHHRI MDKYHPG FGK+GMR+FH
Sbjct: 54 TQLHKNRKKRGHVSAGHGRIGKHRKHPAGRGMAGGQHHHRIMMDKYHPGYFGKVGMRHFH 113
Query: 221 FRKNKNFCPVL 253
KN+ CP +
Sbjct: 114 KLKNQTVCPTI 124
Score = 46.0 bits (104), Expect = 5e-04
Identities = 26/84 (30%), Positives = 38/84 (45%)
Frame = +1
Query: 226 KEQEFLSSFDLDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPKQPVIV 405
K Q + ++DKLWTLV + R + A + PVI++ P QPV+V
Sbjct: 116 KNQTVCPTINIDKLWTLVGTEKREECAKNTS-QAPVIDLTDHGIFKLLGKGQLPNQPVVV 174
Query: 406 XXXXXXXXXXXXXXDVGGACVLSA 477
+VGGAC+L+A
Sbjct: 175 KCRYISKLAEKKIKEVGGACILTA 198
>UniRef50_A1BQ56 Cluster: Ribosomal protein L27a; n=4;
Bilateria|Rep: Ribosomal protein L27a - Ascaris suum
(Pig roundworm) (Ascaris lumbricoides)
Length = 133
Score = 78.2 bits (184), Expect = 1e-13
Identities = 30/40 (75%), Positives = 34/40 (85%)
Frame = +2
Query: 134 NAGGEHHHRINMDKYHPGCFGKLGMRNFHFRKNKNFCPVL 253
NAGG+HHHRIN DKYHPG FGK+GMRNFH RKN +CP +
Sbjct: 20 NAGGQHHHRINFDKYHPGYFGKVGMRNFHVRKNGLYCPTV 59
Score = 64.1 bits (149), Expect = 2e-09
Identities = 37/88 (42%), Positives = 46/88 (52%)
Frame = +1
Query: 214 FPL*KEQEFLSSFDLDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPKQ 393
F + K + + +LDKLW+LVSE+TR YA D K PVI++VKA PKQ
Sbjct: 47 FHVRKNGLYCPTVNLDKLWSLVSEKTRTTYAEKKD-KAPVIDVVKAGYFKVLGKGLLPKQ 105
Query: 394 PVIVXXXXXXXXXXXXXXDVGGACVLSA 477
PVIV VGGAC+L A
Sbjct: 106 PVIVKAKFFSRTAEQKIKGVGGACILVA 133
>UniRef50_Q00454 Cluster: 60S ribosomal protein L27a; n=3;
Eukaryota|Rep: 60S ribosomal protein L27a - Tetrahymena
thermophila
Length = 149
Score = 77.8 bits (183), Expect = 1e-13
Identities = 38/75 (50%), Positives = 44/75 (58%), Gaps = 2/75 (2%)
Frame = +2
Query: 35 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXN--AGGEHHHRINMDKYHPGCFGKLGM 208
M + KKTRKLRGHVS AGG HHHRI M+K+HPG +GKLGM
Sbjct: 1 MVSHLKKTRKLRGHVSHGHGRVGKHRKGGCRGGRGKAGGMHHHRILMEKWHPGYYGKLGM 60
Query: 209 RNFHFRKNKNFCPVL 253
R FH +KN CPV+
Sbjct: 61 RTFHLKKNPLHCPVV 75
Score = 58.0 bits (134), Expect = 1e-07
Identities = 32/75 (42%), Positives = 40/75 (53%)
Frame = +1
Query: 253 DLDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPKQPVIVXXXXXXXXX 432
++DKLW+LVS+ TR KYA KVPVI++ KA P QPV+V
Sbjct: 76 NIDKLWSLVSDATRQKYAE-DKKKVPVIDVTKAGFFKVLGKGRLPNQPVVVKAKYFSKTA 134
Query: 433 XXXXXDVGGACVLSA 477
VGGACVL+A
Sbjct: 135 ERRIVAVGGACVLTA 149
>UniRef50_Q9AW02 Cluster: 60S ribosomal protein L27A; n=3;
Eukaryota|Rep: 60S ribosomal protein L27A - Guillardia
theta (Cryptomonas phi)
Length = 144
Score = 59.7 bits (138), Expect = 4e-08
Identities = 28/69 (40%), Positives = 36/69 (52%)
Frame = +2
Query: 35 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGCFGKLGMRN 214
M T +++RK RGHVS N+GG+HHH+I +KYH FGK GMR
Sbjct: 1 MPTDIRRSRKKRGHVSSGHGRVGKHRKHPGGRGNSGGQHHHKIFFEKYHKKHFGKTGMRY 60
Query: 215 FHFRKNKNF 241
+ K NF
Sbjct: 61 YRKLKQNNF 69
>UniRef50_Q4P992 Cluster: Putative uncharacterized protein; n=1;
Ustilago maydis|Rep: Putative uncharacterized protein -
Ustilago maydis (Smut fungus)
Length = 187
Score = 52.4 bits (120), Expect = 5e-06
Identities = 25/50 (50%), Positives = 26/50 (52%)
Frame = +2
Query: 35 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHP 184
M T KTRK RGHVS AGG+HHHR NMDKYHP
Sbjct: 1 MPTHLSKTRKHRGHVSAGNGRVGKHRKHPGGRGLAGGQHHHRTNMDKYHP 50
Score = 36.3 bits (80), Expect = 0.38
Identities = 27/88 (30%), Positives = 35/88 (39%)
Frame = +1
Query: 214 FPL*KEQEFLSSFDLDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPKQ 393
F L + F +LDKLWTLV + R K +A +VPVI+ + A P
Sbjct: 101 FHLTRNSSFRPVVNLDKLWTLVPAEQR-KGLTADSTEVPVIDTLAAGYGKVLGKGRLPAL 159
Query: 394 PVIVXXXXXXXXXXXXXXDVGGACVLSA 477
P IV + GG L A
Sbjct: 160 PCIVKARWVSSLAEKKIKEAGGVVKLVA 187
Score = 33.1 bits (72), Expect = 3.6
Identities = 12/21 (57%), Positives = 17/21 (80%)
Frame = +2
Query: 191 FGKLGMRNFHFRKNKNFCPVL 253
FGK+GMR+FH +N +F PV+
Sbjct: 93 FGKVGMRHFHLTRNSSFRPVV 113
>UniRef50_O62581 Cluster: 60S ribosomal protein L27a; n=1;
Encephalitozoon cuniculi|Rep: 60S ribosomal protein L27a
- Encephalitozoon cuniculi
Length = 147
Score = 48.8 bits (111), Expect = 7e-05
Identities = 25/69 (36%), Positives = 33/69 (47%)
Frame = +2
Query: 35 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGCFGKLGMRN 214
M KKTRKLRGHVS AGG H + ++HP GK GMR
Sbjct: 1 MTDRVKKTRKLRGHVSHGYGRVGKHRKHSGGRGLAGGFSHMKTFFTRFHPDYHGKRGMRV 60
Query: 215 FHFRKNKNF 241
+H ++N ++
Sbjct: 61 YHRKENSDY 69
>UniRef50_Q3LW97 Cluster: Ribosomal protein L27a; n=1; Bigelowiella
natans|Rep: Ribosomal protein L27a - Bigelowiella natans
(Pedinomonas minutissima) (Chlorarachnion sp.(strain
CCMP 621))
Length = 151
Score = 47.2 bits (107), Expect = 2e-04
Identities = 23/68 (33%), Positives = 32/68 (47%)
Frame = +2
Query: 35 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGCFGKLGMRN 214
M T KK+RK+RG S +GG+HHH+I ++Y F K GMR
Sbjct: 1 MVTKLKKSRKMRGKNSSGHGRINKHRKNPGGKGKSGGQHHHKIIFERYRETYFRKTGMRI 60
Query: 215 FHFRKNKN 238
++ K N
Sbjct: 61 YNLEKTLN 68
>UniRef50_A7QSF4 Cluster: Chromosome chr12 scaffold_159, whole
genome shotgun sequence; n=3; Vitis vinifera|Rep:
Chromosome chr12 scaffold_159, whole genome shotgun
sequence - Vitis vinifera (Grape)
Length = 151
Score = 42.3 bits (95), Expect = 0.006
Identities = 16/28 (57%), Positives = 22/28 (78%)
Frame = +2
Query: 170 DKYHPGCFGKLGMRNFHFRKNKNFCPVL 253
DK+HPG FGK+GMR+FH + K CP++
Sbjct: 28 DKHHPGNFGKVGMRHFHKLRIKLHCPIV 55
>UniRef50_UPI0000DA3FAD Cluster: PREDICTED: similar to 60S ribosomal
protein L27a; n=1; Rattus norvegicus|Rep: PREDICTED:
similar to 60S ribosomal protein L27a - Rattus
norvegicus
Length = 86
Score = 38.7 bits (86), Expect = 0.072
Identities = 21/51 (41%), Positives = 24/51 (47%)
Frame = +2
Query: 35 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPG 187
M+ +KTRK +GHVS NAGGEH HRIN PG
Sbjct: 1 MSGRLRKTRKFQGHVSDSHSRISKHHKHRGGQRNAGGEHLHRINCYYKVPG 51
>UniRef50_A4ANL3 Cluster: Putative lipoprotein; n=1;
Flavobacteriales bacterium HTCC2170|Rep: Putative
lipoprotein - Flavobacteriales bacterium HTCC2170
Length = 535
Score = 33.9 bits (74), Expect = 2.0
Identities = 16/32 (50%), Positives = 19/32 (59%)
Frame = -3
Query: 467 TQAPPTSLIFFSADFEKNFAFTMTGCLGSLPL 372
T A SL FFS D + N A T GCLG+ P+
Sbjct: 306 TDAEGNSLYFFSLDTKDNSACTSAGCLGAWPV 337
>UniRef50_Q9ZQM0 Cluster: Putative non-LTR retroelement reverse
transcriptase; n=1; Arabidopsis thaliana|Rep: Putative
non-LTR retroelement reverse transcriptase - Arabidopsis
thaliana (Mouse-ear cress)
Length = 1044
Score = 33.5 bits (73), Expect = 2.7
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = +1
Query: 169 GQVPSWMLWQTWYEKFPL*KEQEFLSSFDL 258
GQ+P W+LW W + L EQ+ +SS DL
Sbjct: 842 GQLPIWILWNLWNCRNKLIFEQKHISSMDL 871
>UniRef50_Q6AL41 Cluster: Putative uncharacterized protein; n=1;
Desulfotalea psychrophila|Rep: Putative uncharacterized
protein - Desulfotalea psychrophila
Length = 516
Score = 32.3 bits (70), Expect = 6.2
Identities = 19/51 (37%), Positives = 28/51 (54%), Gaps = 1/51 (1%)
Frame = -1
Query: 319 HLEQMHTSASS-VQRLMSKAYLSQNWTEILVLSKVEISHTKFAKASRMVLV 170
H + +T S+ VQR +S YLS T+I EI + KF K +R +L+
Sbjct: 209 HSPKSYTGGSADVQRTISAIYLSDILTQIFFSRTPEIYYNKFRKEARSLLM 259
>UniRef50_A7RPS0 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 306
Score = 31.9 bits (69), Expect = 8.2
Identities = 17/46 (36%), Positives = 23/46 (50%)
Frame = -3
Query: 242 RNSCSF*SGNFSYQVCQSIQDGTCPC*FCDGAHHQHYHDLLDAYGA 105
R+SC + F +C+S T PC D H +H DL+D GA
Sbjct: 108 RSSCQ--ARCFMNNLCRSYNYNTTPCQLSDSDHLEHPSDLVDKPGA 151
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 467,831,051
Number of Sequences: 1657284
Number of extensions: 8758988
Number of successful extensions: 21562
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 20809
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 21541
length of database: 575,637,011
effective HSP length: 95
effective length of database: 418,195,031
effective search space used: 29273652170
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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