BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00294 (499 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g23290.1 68414.m02913 60S ribosomal protein L27A (RPL27aB) si... 87 8e-18 At1g70600.1 68414.m08133 60S ribosomal protein L27A (RPL27aC) id... 84 6e-17 At1g12960.1 68414.m01505 60S ribosomal protein L27A (RPL27aA) si... 29 1.3 At2g16100.1 68415.m01846 hypothetical protein 27 5.3 At2g39930.1 68415.m04907 isoamylase, putative / starch debranchi... 27 9.3 >At1g23290.1 68414.m02913 60S ribosomal protein L27A (RPL27aB) similar to 60S RIBOSOMAL PROTEIN L27A GB:P49637 GI:1710530 from [Arabidopsis thaliana] Length = 146 Score = 86.6 bits (205), Expect = 8e-18 Identities = 40/73 (54%), Positives = 45/73 (61%) Frame = +2 Query: 35 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGCFGKLGMRN 214 MAT+ KK RK RGHVS NAGG HHHRI DKYHPG FGK+GMR Sbjct: 1 MATALKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 60 Query: 215 FHFRKNKNFCPVL 253 FH +NK FCP++ Sbjct: 61 FHKLRNKFFCPIV 73 Score = 33.1 bits (72), Expect = 0.11 Identities = 24/81 (29%), Positives = 37/81 (45%), Gaps = 1/81 (1%) Frame = +1 Query: 238 FLSSFDLDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPK-QPVIVXXX 414 F +LDKLW+LV E + K S+ D VP+I++ + P+ +P +V Sbjct: 69 FCPIVNLDKLWSLVPEDVKAK--SSKD-NVPLIDVTQHGFFKVLGKGHLPENKPFVVKAK 125 Query: 415 XXXXXXXXXXXDVGGACVLSA 477 + GGA VL+A Sbjct: 126 LISKTAEKKIKEAGGAVVLTA 146 >At1g70600.1 68414.m08133 60S ribosomal protein L27A (RPL27aC) identical to 60S ribosomal protein L27A GB:P49637 [Arabidopsis thaliana] Length = 146 Score = 83.8 bits (198), Expect = 6e-17 Identities = 39/73 (53%), Positives = 43/73 (58%) Frame = +2 Query: 35 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGCFGKLGMRN 214 M T KK RK RGHVS NAGG HHHRI DKYHPG FGK+GMR Sbjct: 1 MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 60 Query: 215 FHFRKNKNFCPVL 253 FH +NK FCP++ Sbjct: 61 FHKLRNKFFCPIV 73 Score = 32.7 bits (71), Expect = 0.14 Identities = 24/81 (29%), Positives = 36/81 (44%), Gaps = 1/81 (1%) Frame = +1 Query: 238 FLSSFDLDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPK-QPVIVXXX 414 F +LDKLW+LV E + K S D VP+I++ + P+ +P +V Sbjct: 69 FCPIVNLDKLWSLVPEDVKAK--STKD-NVPLIDVTQHGFFKVLGKGHLPENKPFVVKAK 125 Query: 415 XXXXXXXXXXXDVGGACVLSA 477 + GGA VL+A Sbjct: 126 LISKTAEKKIKEAGGAVVLTA 146 >At1g12960.1 68414.m01505 60S ribosomal protein L27A (RPL27aA) similar to GB:BAA96068 from [Panax ginseng] Length = 104 Score = 29.5 bits (63), Expect = 1.3 Identities = 25/75 (33%), Positives = 32/75 (42%), Gaps = 2/75 (2%) Frame = +2 Query: 35 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGCFGK--LGM 208 M TS+KKTR LR HVS H R + PG G +GM Sbjct: 1 MTTSRKKTRNLREHVSVG---------------------HGRFGKHRKLPGSRGNAGVGM 39 Query: 209 RNFHFRKNKNFCPVL 253 R FH +NK +C ++ Sbjct: 40 RYFHKLRNKFYCQIV 54 >At2g16100.1 68415.m01846 hypothetical protein Length = 250 Score = 27.5 bits (58), Expect = 5.3 Identities = 8/19 (42%), Positives = 13/19 (68%) Frame = +1 Query: 148 APSQNQHGQVPSWMLWQTW 204 +P +Q Q+P W+LW+ W Sbjct: 20 SPRTSQFHQLPLWILWRIW 38 >At2g39930.1 68415.m04907 isoamylase, putative / starch debranching enzyme, putative similar to isoamylase from [Solanum tuberosum] GI:27728145, [Triticum aestivum] GI:17932898, [Hordeum vulgare] GI:21314275, [Oryza sativa] GI:3252794; contains Pfam profiles PF00128: Alpha amylase catalytic domain, PF02922: Isoamylase N-terminal domain Length = 783 Score = 26.6 bits (56), Expect = 9.3 Identities = 10/25 (40%), Positives = 11/25 (44%) Frame = +1 Query: 142 W*APSQNQHGQVPSWMLWQTWYEKF 216 W A Q G P W +W W KF Sbjct: 468 WDAGGLYQVGMFPHWGIWSEWNGKF 492 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,301,241 Number of Sequences: 28952 Number of extensions: 203392 Number of successful extensions: 531 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 510 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 530 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 878448512 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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