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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00294
         (499 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g23290.1 68414.m02913 60S ribosomal protein L27A (RPL27aB) si...    87   8e-18
At1g70600.1 68414.m08133 60S ribosomal protein L27A (RPL27aC) id...    84   6e-17
At1g12960.1 68414.m01505 60S ribosomal protein L27A (RPL27aA) si...    29   1.3  
At2g16100.1 68415.m01846 hypothetical protein                          27   5.3  
At2g39930.1 68415.m04907 isoamylase, putative / starch debranchi...    27   9.3  

>At1g23290.1 68414.m02913 60S ribosomal protein L27A (RPL27aB)
           similar to 60S RIBOSOMAL PROTEIN L27A GB:P49637
           GI:1710530 from [Arabidopsis thaliana]
          Length = 146

 Score = 86.6 bits (205), Expect = 8e-18
 Identities = 40/73 (54%), Positives = 45/73 (61%)
 Frame = +2

Query: 35  MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGCFGKLGMRN 214
           MAT+ KK RK RGHVS                 NAGG HHHRI  DKYHPG FGK+GMR 
Sbjct: 1   MATALKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 60

Query: 215 FHFRKNKNFCPVL 253
           FH  +NK FCP++
Sbjct: 61  FHKLRNKFFCPIV 73



 Score = 33.1 bits (72), Expect = 0.11
 Identities = 24/81 (29%), Positives = 37/81 (45%), Gaps = 1/81 (1%)
 Frame = +1

Query: 238 FLSSFDLDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPK-QPVIVXXX 414
           F    +LDKLW+LV E  + K  S+ D  VP+I++ +            P+ +P +V   
Sbjct: 69  FCPIVNLDKLWSLVPEDVKAK--SSKD-NVPLIDVTQHGFFKVLGKGHLPENKPFVVKAK 125

Query: 415 XXXXXXXXXXXDVGGACVLSA 477
                      + GGA VL+A
Sbjct: 126 LISKTAEKKIKEAGGAVVLTA 146


>At1g70600.1 68414.m08133 60S ribosomal protein L27A (RPL27aC)
           identical to 60S ribosomal protein L27A GB:P49637
           [Arabidopsis thaliana]
          Length = 146

 Score = 83.8 bits (198), Expect = 6e-17
 Identities = 39/73 (53%), Positives = 43/73 (58%)
 Frame = +2

Query: 35  MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGCFGKLGMRN 214
           M T  KK RK RGHVS                 NAGG HHHRI  DKYHPG FGK+GMR 
Sbjct: 1   MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 60

Query: 215 FHFRKNKNFCPVL 253
           FH  +NK FCP++
Sbjct: 61  FHKLRNKFFCPIV 73



 Score = 32.7 bits (71), Expect = 0.14
 Identities = 24/81 (29%), Positives = 36/81 (44%), Gaps = 1/81 (1%)
 Frame = +1

Query: 238 FLSSFDLDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPK-QPVIVXXX 414
           F    +LDKLW+LV E  + K  S  D  VP+I++ +            P+ +P +V   
Sbjct: 69  FCPIVNLDKLWSLVPEDVKAK--STKD-NVPLIDVTQHGFFKVLGKGHLPENKPFVVKAK 125

Query: 415 XXXXXXXXXXXDVGGACVLSA 477
                      + GGA VL+A
Sbjct: 126 LISKTAEKKIKEAGGAVVLTA 146


>At1g12960.1 68414.m01505 60S ribosomal protein L27A (RPL27aA)
           similar to GB:BAA96068 from [Panax ginseng]
          Length = 104

 Score = 29.5 bits (63), Expect = 1.3
 Identities = 25/75 (33%), Positives = 32/75 (42%), Gaps = 2/75 (2%)
 Frame = +2

Query: 35  MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGCFGK--LGM 208
           M TS+KKTR LR HVS                       H R    +  PG  G   +GM
Sbjct: 1   MTTSRKKTRNLREHVSVG---------------------HGRFGKHRKLPGSRGNAGVGM 39

Query: 209 RNFHFRKNKNFCPVL 253
           R FH  +NK +C ++
Sbjct: 40  RYFHKLRNKFYCQIV 54


>At2g16100.1 68415.m01846 hypothetical protein 
          Length = 250

 Score = 27.5 bits (58), Expect = 5.3
 Identities = 8/19 (42%), Positives = 13/19 (68%)
 Frame = +1

Query: 148 APSQNQHGQVPSWMLWQTW 204
           +P  +Q  Q+P W+LW+ W
Sbjct: 20  SPRTSQFHQLPLWILWRIW 38


>At2g39930.1 68415.m04907 isoamylase, putative / starch debranching
           enzyme, putative similar to isoamylase from [Solanum
           tuberosum] GI:27728145, [Triticum aestivum] GI:17932898,
           [Hordeum vulgare] GI:21314275, [Oryza sativa]
           GI:3252794; contains Pfam profiles PF00128: Alpha
           amylase catalytic domain, PF02922: Isoamylase N-terminal
           domain
          Length = 783

 Score = 26.6 bits (56), Expect = 9.3
 Identities = 10/25 (40%), Positives = 11/25 (44%)
 Frame = +1

Query: 142 W*APSQNQHGQVPSWMLWQTWYEKF 216
           W A    Q G  P W +W  W  KF
Sbjct: 468 WDAGGLYQVGMFPHWGIWSEWNGKF 492


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,301,241
Number of Sequences: 28952
Number of extensions: 203392
Number of successful extensions: 531
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 510
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 530
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 878448512
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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