BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00289 (636 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1682.14 |rpl1902|rpl19-2|60S ribosomal protein L19B|Schizosa... 128 9e-31 SPBC56F2.02 |rpl1901|rpl19-1|60S ribosomal protein L19|Schizosac... 128 9e-31 SPAC23C11.03 |||U3 snoRNP-associated protein Mpp1 |Schizosacchar... 29 0.56 SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharo... 29 0.56 SPBC28E12.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 29 0.74 SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 27 3.0 SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex su... 26 5.2 SPBC21.05c |ral2||Ras guanyl-nucleotide exchange factor Ral2 |Sc... 26 5.2 SPAC3H8.02 |||sec14 cytosolic factor family|Schizosaccharomyces ... 25 9.1 SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ... 25 9.1 SPAC10F6.03c |||CTP synthase |Schizosaccharomyces pombe|chr 1|||... 25 9.1 >SPCC1682.14 |rpl1902|rpl19-2|60S ribosomal protein L19B|Schizosaccharomyces pombe|chr 3|||Manual Length = 193 Score = 128 bits (308), Expect = 9e-31 Identities = 55/82 (67%), Positives = 72/82 (87%) Frame = +3 Query: 12 MSSLKLQKRLAASVMRCGKKKVWLDPNEINEIANTNSRQNIRKMIKDGLVIKKPVAVHSR 191 M++L+ QKRLAASV++CGK+KVW+DPNEI+EI+N NSRQNIRK++KDGLVI+KP +HSR Sbjct: 1 MANLRTQKRLAASVLKCGKRKVWMDPNEISEISNANSRQNIRKLVKDGLVIRKPNLMHSR 60 Query: 192 ARVRKNTEARRKGRHCGFGKRE 257 R+RK A+R GRH G+GKR+ Sbjct: 61 FRIRKTHAAKRLGRHTGYGKRK 82 Score = 87.0 bits (206), Expect = 2e-18 Identities = 39/86 (45%), Positives = 55/86 (63%) Frame = +2 Query: 254 RGTANARMPQKELWXXXXXXXXXXXXXXXTAKKIDRHLYHSLYMKAKGNVFKNKRVLMEY 433 +GTA ARMP +W + KID+HLYH+LY++AKGN FK+KR L+E+ Sbjct: 82 KGTAEARMPSTVVWMRRQRVLRRLLRKYRESGKIDKHLYHTLYLEAKGNTFKHKRALIEH 141 Query: 434 IHRKKAEKARTKMLSDQAEARRNKVR 511 I R KAE RTK++ +Q +ARR + + Sbjct: 142 IQRAKAEANRTKLIQEQQDARRARAK 167 >SPBC56F2.02 |rpl1901|rpl19-1|60S ribosomal protein L19|Schizosaccharomyces pombe|chr 2|||Manual Length = 193 Score = 128 bits (308), Expect = 9e-31 Identities = 55/82 (67%), Positives = 72/82 (87%) Frame = +3 Query: 12 MSSLKLQKRLAASVMRCGKKKVWLDPNEINEIANTNSRQNIRKMIKDGLVIKKPVAVHSR 191 M++L+ QKRLAASV++CGK+KVW+DPNEI+EI+N NSRQN+RK+IKDGLVI+KP +HSR Sbjct: 1 MANLRTQKRLAASVLKCGKRKVWMDPNEISEISNANSRQNVRKLIKDGLVIRKPNLMHSR 60 Query: 192 ARVRKNTEARRKGRHCGFGKRE 257 R+RK A+R GRH G+GKR+ Sbjct: 61 FRIRKTHAAKRLGRHTGYGKRK 82 Score = 87.0 bits (206), Expect = 2e-18 Identities = 39/86 (45%), Positives = 55/86 (63%) Frame = +2 Query: 254 RGTANARMPQKELWXXXXXXXXXXXXXXXTAKKIDRHLYHSLYMKAKGNVFKNKRVLMEY 433 +GTA ARMP +W + KID+HLYH+LY++AKGN FK+KR L+E+ Sbjct: 82 KGTAEARMPSAVVWMRRQRVLRRLLRKYRESGKIDKHLYHTLYLEAKGNTFKHKRALIEH 141 Query: 434 IHRKKAEKARTKMLSDQAEARRNKVR 511 I R KAE RTK++ +Q +ARR + + Sbjct: 142 IQRAKAEANRTKLIQEQQDARRARAK 167 >SPAC23C11.03 |||U3 snoRNP-associated protein Mpp1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 598 Score = 29.1 bits (62), Expect = 0.56 Identities = 16/44 (36%), Positives = 23/44 (52%) Frame = +2 Query: 428 EYIHRKKAEKARTKMLSDQAEARRNKVRRHASAARNVLPPRRRN 559 E I R + T+M Q +ARR +VRR + R + +RRN Sbjct: 510 ETITRSGIAISSTEMDHQQKQARRRRVRRKHAEKRKQMAEKRRN 553 >SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharomyces pombe|chr 2|||Manual Length = 1309 Score = 29.1 bits (62), Expect = 0.56 Identities = 12/30 (40%), Positives = 21/30 (70%) Frame = -2 Query: 155 TILDHLTDVLSGVGVCDLIDFIWIQPHLLF 66 +I +H++DV G+ V LI+ + I+P +LF Sbjct: 351 SICEHVSDVSRGIAVETLIELMIIRPKILF 380 >SPBC28E12.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 356 Score = 28.7 bits (61), Expect = 0.74 Identities = 12/32 (37%), Positives = 19/32 (59%) Frame = -2 Query: 134 DVLSGVGVCDLIDFIWIQPHLLFTTSHNRGCK 39 +VLS + ++D + PHLL +S N+ CK Sbjct: 237 EVLSKLPYFHILDKSFFSPHLLLQSSENQACK 268 >SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1|||Manual Length = 1841 Score = 26.6 bits (56), Expect = 3.0 Identities = 20/73 (27%), Positives = 33/73 (45%) Frame = +2 Query: 395 GNVFKNKRVLMEYIHRKKAEKARTKMLSDQAEARRNKVRRHASAARNVLPPRRRNCCRPS 574 G + K + V +E H+ + A T EA + +S A+N L +R+ + Sbjct: 1414 GFLDKLRNVKLESHHKPRNRSAITMGKEHLIEAPNTSTK--SSPAKNELFVPKRSSVKSD 1471 Query: 575 LEKTKPRLPLRSK 613 L K +PR P S+ Sbjct: 1472 LAKVRPRYPKGSE 1484 >SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex subunit Mtr4|Schizosaccharomyces pombe|chr 1|||Manual Length = 1117 Score = 25.8 bits (54), Expect = 5.2 Identities = 19/80 (23%), Positives = 40/80 (50%), Gaps = 3/80 (3%) Frame = +2 Query: 344 AKKIDRHLYHSLYMKAKGNVFKNKRVLMEYIH--RKKAEKARTKMLSDQAEARRNKVRRH 517 ++ + L++ ++ K + K L+E + +KK KAR+ M D+ +R+ +RR Sbjct: 876 SRLLSNPLHNFSELEEKYAEYLRKLALLEEVKDLKKKLSKARSIMQLDELNSRKRVLRRL 935 Query: 518 A-SAARNVLPPRRRNCCRPS 574 + + +V+ + R C S Sbjct: 936 GFTTSDDVIEVKGRVACEIS 955 >SPBC21.05c |ral2||Ras guanyl-nucleotide exchange factor Ral2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 611 Score = 25.8 bits (54), Expect = 5.2 Identities = 11/32 (34%), Positives = 17/32 (53%), Gaps = 1/32 (3%) Frame = -1 Query: 450 AFFLWMYSMSTRLFLNTLPFAFM-*SEWYRCL 358 AF LW+YS+ L+L + W++CL Sbjct: 337 AFVLWVYSLDKELWLQLDMLGVLNHGSWFKCL 368 >SPAC3H8.02 |||sec14 cytosolic factor family|Schizosaccharomyces pombe|chr 1|||Manual Length = 444 Score = 25.0 bits (52), Expect = 9.1 Identities = 16/65 (24%), Positives = 31/65 (47%), Gaps = 11/65 (16%) Frame = +3 Query: 72 KVWLDPNEINEIANTNSRQNIRKMIKDGLVIKK-----------PVAVHSRARVRKNTEA 218 K WLDP ++++ T + +++++ I ++K+ P + A KN EA Sbjct: 289 KSWLDPVVVSKVKFTRNYRDLQQYINPDNILKEFGGPNPWRYTYPEPCQNEAEALKNVEA 348 Query: 219 RRKGR 233 R+ R Sbjct: 349 RKSLR 353 >SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 25.0 bits (52), Expect = 9.1 Identities = 11/39 (28%), Positives = 20/39 (51%) Frame = +3 Query: 126 QNIRKMIKDGLVIKKPVAVHSRARVRKNTEARRKGRHCG 242 +++R +KD + +PV+V S A ++T G G Sbjct: 141 ESLRSKLKDAMASTEPVSVDSAAAPSQSTNPEGNGGSVG 179 >SPAC10F6.03c |||CTP synthase |Schizosaccharomyces pombe|chr 1|||Manual Length = 600 Score = 25.0 bits (52), Expect = 9.1 Identities = 13/44 (29%), Positives = 22/44 (50%), Gaps = 2/44 (4%) Frame = +3 Query: 15 SSLKLQKRLAASVMRCGKK--KVWLDPNEINEIANTNSRQNIRK 140 S + + K L S MRCG+K W++ + + NT+ + K Sbjct: 317 SYISVIKALEHSAMRCGRKLDLQWVEASHLEASTNTSDPLSYHK 360 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,503,091 Number of Sequences: 5004 Number of extensions: 50424 Number of successful extensions: 163 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 152 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 161 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 283719918 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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