BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00288 (664 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_53271| Best HMM Match : No HMM Matches (HMM E-Value=.) 147 8e-36 SB_56329| Best HMM Match : DUF112 (HMM E-Value=4.3) 29 3.4 SB_11242| Best HMM Match : MAM (HMM E-Value=0) 29 4.5 SB_46368| Best HMM Match : DUF156 (HMM E-Value=6.5) 28 5.9 SB_41068| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 7.8 >SB_53271| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 687 Score = 147 bits (356), Expect = 8e-36 Identities = 66/75 (88%), Positives = 70/75 (93%) Frame = +1 Query: 31 MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNE 210 MARGPKKH+KRLNAPK WMLDKL GV+APRPSTGPHKLRECLPL+IFLRNRLKYAL G E Sbjct: 425 MARGPKKHMKRLNAPKHWMLDKLSGVFAPRPSTGPHKLRECLPLIIFLRNRLKYALNGEE 484 Query: 211 VLKIVKQRLIKVDGK 255 V KIVKQRLIK+DGK Sbjct: 485 VKKIVKQRLIKIDGK 499 Score = 142 bits (345), Expect = 2e-34 Identities = 64/86 (74%), Positives = 74/86 (86%) Frame = +3 Query: 252 EVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPK 431 +VRTD TYPAGFMDVV+I+KT E FRL+YDVKGRF +HRIT EEAKYKL +V+RV G K Sbjct: 499 KVRTDTTYPAGFMDVVTIDKTGENFRLLYDVKGRFAVHRITAEEAKYKLGRVRRVDVGAK 558 Query: 432 NVPYLVTHDGRTIRYPDPLIKVNDSI 509 VPY+VTHD RTIRYPDP IKVND++ Sbjct: 559 GVPYIVTHDARTIRYPDPNIKVNDTV 584 Score = 52.0 bits (119), Expect = 4e-07 Identities = 21/44 (47%), Positives = 31/44 (70%) Frame = +2 Query: 476 PRPTYQSQRFHQLDIATTKIMDFIKFESGNLCMITGGRNLGRVG 607 P P + +DI T K++D+IKF++GN+ M+ GGRN+GRVG Sbjct: 574 PDPNIKVNDTVVIDIKTGKVIDYIKFDTGNMAMVVGGRNMGRVG 617 Score = 37.5 bits (83), Expect = 0.010 Identities = 14/24 (58%), Positives = 18/24 (75%) Frame = +1 Query: 592 LGACGHIVSRERHPGSFDIVHIKD 663 +G G + RE+H GSFDIVH+KD Sbjct: 613 MGRVGMVTHREKHAGSFDIVHVKD 636 >SB_56329| Best HMM Match : DUF112 (HMM E-Value=4.3) Length = 573 Score = 29.1 bits (62), Expect = 3.4 Identities = 27/95 (28%), Positives = 43/95 (45%), Gaps = 1/95 (1%) Frame = -1 Query: 412 RLTLHNLYLASSGVIRWIVNLPLTS*IRRNSSLVFSIETTSINPAG*VGSVLTSHQPL*G 233 R +++AS VI I ++ IR + SL+ S + N G + P+ G Sbjct: 320 RFGARQVFIASRHVITTIPSMHRARYIRDSGSLLSSAVESYANYFGIIQLCSIGFGPILG 379 Query: 232 VVSQFSGLRFRSK-HTSDDSSGKSPGASTRATCGD 131 V SG R + ++DDSS +++ ATC D Sbjct: 380 FVIS-SGKRVSKRLDSTDDSSSMIIQSNSSATCKD 413 >SB_11242| Best HMM Match : MAM (HMM E-Value=0) Length = 348 Score = 28.7 bits (61), Expect = 4.5 Identities = 12/20 (60%), Positives = 16/20 (80%), Gaps = 2/20 (10%) Frame = +3 Query: 174 EES--SEVCFDRKRSPENCE 227 EES +E+C DRKR P++CE Sbjct: 76 EESRYNELCHDRKRGPDDCE 95 >SB_46368| Best HMM Match : DUF156 (HMM E-Value=6.5) Length = 203 Score = 28.3 bits (60), Expect = 5.9 Identities = 18/52 (34%), Positives = 25/52 (48%), Gaps = 1/52 (1%) Frame = -3 Query: 173 RKITRGKHSRNLWGPVDGLGAYTPPSLSNIHALGAF-KRFKCFLGPRAMLDK 21 RK +R N++G ++GLG+ PP + I F K K F PR K Sbjct: 60 RKRSRSLPRTNVFGTLNGLGSPPPPRDNRIDEYAEFTKSQKSFTFPRRSKQK 111 >SB_41068| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 141 Score = 27.9 bits (59), Expect = 7.8 Identities = 15/45 (33%), Positives = 22/45 (48%), Gaps = 6/45 (13%) Frame = -3 Query: 251 PSTFMRRCFTIFRTSFPVKAYFRRFLRK------ITRGKHSRNLW 135 PS++ F +FRT FP + RF R+ IT ++LW Sbjct: 84 PSSYNGHQFLVFRTDFPFSKHKNRFKRRTKYLYVITTSTKHQHLW 128 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 22,923,245 Number of Sequences: 59808 Number of extensions: 494660 Number of successful extensions: 1182 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 1096 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1181 length of database: 16,821,457 effective HSP length: 79 effective length of database: 12,096,625 effective search space used: 1705624125 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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