BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00288 (664 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) riboso... 137 5e-33 At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) 137 5e-33 At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contai... 137 5e-33 At4g10710.1 68417.m01751 transcriptional regulator-related simil... 31 0.68 At3g61220.1 68416.m06851 short-chain dehydrogenase/reductase (SD... 30 1.2 At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase, ... 29 2.8 At5g61460.1 68418.m07712 structural maintenance of chromosomes (... 27 8.4 At5g03980.1 68418.m00378 GDSL-motif lipase/hydrolase family prot... 27 8.4 At2g38570.1 68415.m04738 expressed protein ; expression supporte... 27 8.4 >At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) ribosomal protein S4, Arabidopsis thaliana, PIR:T48480 Length = 262 Score = 137 bits (332), Expect = 5e-33 Identities = 62/86 (72%), Positives = 71/86 (82%) Frame = +3 Query: 252 EVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPK 431 +VRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K Sbjct: 75 KVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQK 134 Query: 432 NVPYLVTHDGRTIRYPDPLIKVNDSI 509 +PYL T+DGRTIRYPDPLIK ND+I Sbjct: 135 GIPYLNTYDGRTIRYPDPLIKPNDTI 160 Score = 126 bits (304), Expect = 1e-29 Identities = 57/75 (76%), Positives = 65/75 (86%) Frame = +1 Query: 31 MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNE 210 MARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT E Sbjct: 1 MARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYRE 60 Query: 211 VLKIVKQRLIKVDGK 255 V+ I+ QR I+VDGK Sbjct: 61 VISILMQRHIQVDGK 75 Score = 50.4 bits (115), Expect = 1e-06 Identities = 22/44 (50%), Positives = 30/44 (68%) Frame = +2 Query: 476 PRPTYQSQRFHQLDIATTKIMDFIKFESGNLCMITGGRNLGRVG 607 P P + +LD+ KI++FIKF+ GN+ M+TGGRN GRVG Sbjct: 150 PDPLIKPNDTIKLDLEANKIVEFIKFDVGNVVMVTGGRNRGRVG 193 Score = 31.5 bits (68), Expect = 0.52 Identities = 12/23 (52%), Positives = 17/23 (73%) Frame = +1 Query: 595 GACGHIVSRERHPGSFDIVHIKD 663 G G I +RE+H GSF+ +HI+D Sbjct: 190 GRVGVIKNREKHKGSFETIHIQD 212 >At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) Length = 262 Score = 137 bits (332), Expect = 5e-33 Identities = 62/86 (72%), Positives = 71/86 (82%) Frame = +3 Query: 252 EVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPK 431 +VRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K Sbjct: 75 KVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQK 134 Query: 432 NVPYLVTHDGRTIRYPDPLIKVNDSI 509 +PYL T+DGRTIRYPDPLIK ND+I Sbjct: 135 GIPYLNTYDGRTIRYPDPLIKPNDTI 160 Score = 126 bits (304), Expect = 1e-29 Identities = 57/75 (76%), Positives = 65/75 (86%) Frame = +1 Query: 31 MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNE 210 MARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT E Sbjct: 1 MARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYRE 60 Query: 211 VLKIVKQRLIKVDGK 255 V+ I+ QR I+VDGK Sbjct: 61 VISILMQRHIQVDGK 75 Score = 50.0 bits (114), Expect = 1e-06 Identities = 22/44 (50%), Positives = 30/44 (68%) Frame = +2 Query: 476 PRPTYQSQRFHQLDIATTKIMDFIKFESGNLCMITGGRNLGRVG 607 P P + +LD+ KI++FIKF+ GN+ M+TGGRN GRVG Sbjct: 150 PDPLIKPNDTIKLDLEENKIVEFIKFDVGNVVMVTGGRNRGRVG 193 Score = 31.5 bits (68), Expect = 0.52 Identities = 12/23 (52%), Positives = 17/23 (73%) Frame = +1 Query: 595 GACGHIVSRERHPGSFDIVHIKD 663 G G I +RE+H GSF+ +HI+D Sbjct: 190 GRVGVIKNREKHKGSFETIHIQD 212 >At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contains ribosomal protein S4 signature from residues 8 to 22 Length = 261 Score = 137 bits (332), Expect = 5e-33 Identities = 62/86 (72%), Positives = 71/86 (82%) Frame = +3 Query: 252 EVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPK 431 +VRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K Sbjct: 75 KVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQK 134 Query: 432 NVPYLVTHDGRTIRYPDPLIKVNDSI 509 +PYL T+DGRTIRYPDPLIK ND+I Sbjct: 135 GIPYLNTYDGRTIRYPDPLIKPNDTI 160 Score = 126 bits (304), Expect = 1e-29 Identities = 57/75 (76%), Positives = 65/75 (86%) Frame = +1 Query: 31 MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNE 210 MARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT E Sbjct: 1 MARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYRE 60 Query: 211 VLKIVKQRLIKVDGK 255 V+ I+ QR I+VDGK Sbjct: 61 VISILMQRHIQVDGK 75 Score = 50.0 bits (114), Expect = 1e-06 Identities = 22/44 (50%), Positives = 30/44 (68%) Frame = +2 Query: 476 PRPTYQSQRFHQLDIATTKIMDFIKFESGNLCMITGGRNLGRVG 607 P P + +LD+ KI++FIKF+ GN+ M+TGGRN GRVG Sbjct: 150 PDPLIKPNDTIKLDLEENKIVEFIKFDVGNVVMVTGGRNRGRVG 193 Score = 31.5 bits (68), Expect = 0.52 Identities = 12/23 (52%), Positives = 17/23 (73%) Frame = +1 Query: 595 GACGHIVSRERHPGSFDIVHIKD 663 G G I +RE+H GSF+ +HI+D Sbjct: 190 GRVGVIKNREKHKGSFETIHIQD 212 >At4g10710.1 68417.m01751 transcriptional regulator-related similar to chromatin-specific transcription elongation factor FACT 140 kDa subunit (GI:5499741) [Homo sapiens] Length = 1074 Score = 31.1 bits (67), Expect = 0.68 Identities = 12/27 (44%), Positives = 16/27 (59%) Frame = -3 Query: 155 KHSRNLWGPVDGLGAYTPPSLSNIHAL 75 KHS +LWG D L TPP+ ++ L Sbjct: 44 KHSADLWGSADALAIATPPASDDLRYL 70 >At3g61220.1 68416.m06851 short-chain dehydrogenase/reductase (SDR) family protein similar to carbonyl reductase GI:1049108 from [Mus musculus] Length = 296 Score = 30.3 bits (65), Expect = 1.2 Identities = 16/48 (33%), Positives = 25/48 (52%), Gaps = 4/48 (8%) Frame = +2 Query: 491 QSQRFHQLDIA----TTKIMDFIKFESGNLCMITGGRNLGRVGTSCPA 622 QS FHQLD+A T + +F+K + G L ++ +G + T A Sbjct: 59 QSLLFHQLDVADPASITSLAEFVKTQFGKLDILVNNAGIGGIITDAEA 106 >At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase, putative / steroid 5-alpha-reductase, putative identical to gi:1280611; contains a steroid 5-alpha reductase, C-terminal domain Length = 262 Score = 29.1 bits (62), Expect = 2.8 Identities = 15/39 (38%), Positives = 19/39 (48%) Frame = -3 Query: 245 TFMRRCFTIFRTSFPVKAYFRRFLRKITRGKHSRNLWGP 129 TF R C + P A +FL+ GKH+R WGP Sbjct: 8 TFFRYCLLTLIFAGPPTAVLLKFLQA-PYGKHNRTGWGP 45 >At5g61460.1 68418.m07712 structural maintenance of chromosomes (SMC) family protein very strong similarity to SMC-like protein (MIM) [Arabidopsis thaliana] GI:5880614; contains Pfam profile PF02463: RecF/RecN/SMC N terminal domain Length = 1057 Score = 27.5 bits (58), Expect = 8.4 Identities = 10/29 (34%), Positives = 16/29 (55%) Frame = +1 Query: 565 LVYDHGRP*LGACGHIVSRERHPGSFDIV 651 ++YD RP L H+V + HP F ++ Sbjct: 532 IIYDFSRPRLNIPRHMVPQTEHPTIFSVI 560 >At5g03980.1 68418.m00378 GDSL-motif lipase/hydrolase family protein similar to lipase [Arabidopsis thaliana] GI:1145627; contains Pfam profile:PF00657 Lipase/Acylhydrolase with GDSL-like motif Length = 323 Score = 27.5 bits (58), Expect = 8.4 Identities = 13/28 (46%), Positives = 15/28 (53%) Frame = -3 Query: 278 RISRVSPNFPSTFMRRCFTIFRTSFPVK 195 R V+ P F CF I+ TSFPVK Sbjct: 166 RAGAVNVVVPGNFPVGCFPIYLTSFPVK 193 >At2g38570.1 68415.m04738 expressed protein ; expression supported by MPSS Length = 302 Score = 27.5 bits (58), Expect = 8.4 Identities = 12/28 (42%), Positives = 17/28 (60%) Frame = +2 Query: 527 TKIMDFIKFESGNLCMITGGRNLGRVGT 610 T I+ +IK G + GGRN+G+V T Sbjct: 196 TYILVYIKISVGYRVVTPGGRNIGKVST 223 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,655,619 Number of Sequences: 28952 Number of extensions: 335511 Number of successful extensions: 938 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 898 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 938 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1393347168 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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