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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00288
         (664 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) riboso...   137   5e-33
At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B)             137   5e-33
At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contai...   137   5e-33
At4g10710.1 68417.m01751 transcriptional regulator-related simil...    31   0.68 
At3g61220.1 68416.m06851 short-chain dehydrogenase/reductase (SD...    30   1.2  
At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase, ...    29   2.8  
At5g61460.1 68418.m07712 structural maintenance of chromosomes (...    27   8.4  
At5g03980.1 68418.m00378 GDSL-motif lipase/hydrolase family prot...    27   8.4  
At2g38570.1 68415.m04738 expressed protein ; expression supporte...    27   8.4  

>At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) ribosomal
           protein S4, Arabidopsis thaliana, PIR:T48480
          Length = 262

 Score =  137 bits (332), Expect = 5e-33
 Identities = 62/86 (72%), Positives = 71/86 (82%)
 Frame = +3

Query: 252 EVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPK 431
           +VRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I  EEAK+KLCKV+ +  G K
Sbjct: 75  KVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQK 134

Query: 432 NVPYLVTHDGRTIRYPDPLIKVNDSI 509
            +PYL T+DGRTIRYPDPLIK ND+I
Sbjct: 135 GIPYLNTYDGRTIRYPDPLIKPNDTI 160



 Score =  126 bits (304), Expect = 1e-29
 Identities = 57/75 (76%), Positives = 65/75 (86%)
 Frame = +1

Query: 31  MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNE 210
           MARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT  E
Sbjct: 1   MARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYRE 60

Query: 211 VLKIVKQRLIKVDGK 255
           V+ I+ QR I+VDGK
Sbjct: 61  VISILMQRHIQVDGK 75



 Score = 50.4 bits (115), Expect = 1e-06
 Identities = 22/44 (50%), Positives = 30/44 (68%)
 Frame = +2

Query: 476 PRPTYQSQRFHQLDIATTKIMDFIKFESGNLCMITGGRNLGRVG 607
           P P  +     +LD+   KI++FIKF+ GN+ M+TGGRN GRVG
Sbjct: 150 PDPLIKPNDTIKLDLEANKIVEFIKFDVGNVVMVTGGRNRGRVG 193



 Score = 31.5 bits (68), Expect = 0.52
 Identities = 12/23 (52%), Positives = 17/23 (73%)
 Frame = +1

Query: 595 GACGHIVSRERHPGSFDIVHIKD 663
           G  G I +RE+H GSF+ +HI+D
Sbjct: 190 GRVGVIKNREKHKGSFETIHIQD 212


>At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B)
          Length = 262

 Score =  137 bits (332), Expect = 5e-33
 Identities = 62/86 (72%), Positives = 71/86 (82%)
 Frame = +3

Query: 252 EVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPK 431
           +VRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I  EEAK+KLCKV+ +  G K
Sbjct: 75  KVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQK 134

Query: 432 NVPYLVTHDGRTIRYPDPLIKVNDSI 509
            +PYL T+DGRTIRYPDPLIK ND+I
Sbjct: 135 GIPYLNTYDGRTIRYPDPLIKPNDTI 160



 Score =  126 bits (304), Expect = 1e-29
 Identities = 57/75 (76%), Positives = 65/75 (86%)
 Frame = +1

Query: 31  MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNE 210
           MARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT  E
Sbjct: 1   MARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYRE 60

Query: 211 VLKIVKQRLIKVDGK 255
           V+ I+ QR I+VDGK
Sbjct: 61  VISILMQRHIQVDGK 75



 Score = 50.0 bits (114), Expect = 1e-06
 Identities = 22/44 (50%), Positives = 30/44 (68%)
 Frame = +2

Query: 476 PRPTYQSQRFHQLDIATTKIMDFIKFESGNLCMITGGRNLGRVG 607
           P P  +     +LD+   KI++FIKF+ GN+ M+TGGRN GRVG
Sbjct: 150 PDPLIKPNDTIKLDLEENKIVEFIKFDVGNVVMVTGGRNRGRVG 193



 Score = 31.5 bits (68), Expect = 0.52
 Identities = 12/23 (52%), Positives = 17/23 (73%)
 Frame = +1

Query: 595 GACGHIVSRERHPGSFDIVHIKD 663
           G  G I +RE+H GSF+ +HI+D
Sbjct: 190 GRVGVIKNREKHKGSFETIHIQD 212


>At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contains
           ribosomal protein S4 signature from residues 8 to 22
          Length = 261

 Score =  137 bits (332), Expect = 5e-33
 Identities = 62/86 (72%), Positives = 71/86 (82%)
 Frame = +3

Query: 252 EVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPK 431
           +VRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I  EEAK+KLCKV+ +  G K
Sbjct: 75  KVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQK 134

Query: 432 NVPYLVTHDGRTIRYPDPLIKVNDSI 509
            +PYL T+DGRTIRYPDPLIK ND+I
Sbjct: 135 GIPYLNTYDGRTIRYPDPLIKPNDTI 160



 Score =  126 bits (304), Expect = 1e-29
 Identities = 57/75 (76%), Positives = 65/75 (86%)
 Frame = +1

Query: 31  MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNE 210
           MARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT  E
Sbjct: 1   MARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYRE 60

Query: 211 VLKIVKQRLIKVDGK 255
           V+ I+ QR I+VDGK
Sbjct: 61  VISILMQRHIQVDGK 75



 Score = 50.0 bits (114), Expect = 1e-06
 Identities = 22/44 (50%), Positives = 30/44 (68%)
 Frame = +2

Query: 476 PRPTYQSQRFHQLDIATTKIMDFIKFESGNLCMITGGRNLGRVG 607
           P P  +     +LD+   KI++FIKF+ GN+ M+TGGRN GRVG
Sbjct: 150 PDPLIKPNDTIKLDLEENKIVEFIKFDVGNVVMVTGGRNRGRVG 193



 Score = 31.5 bits (68), Expect = 0.52
 Identities = 12/23 (52%), Positives = 17/23 (73%)
 Frame = +1

Query: 595 GACGHIVSRERHPGSFDIVHIKD 663
           G  G I +RE+H GSF+ +HI+D
Sbjct: 190 GRVGVIKNREKHKGSFETIHIQD 212


>At4g10710.1 68417.m01751 transcriptional regulator-related similar
           to chromatin-specific transcription elongation factor
           FACT 140 kDa subunit (GI:5499741) [Homo sapiens]
          Length = 1074

 Score = 31.1 bits (67), Expect = 0.68
 Identities = 12/27 (44%), Positives = 16/27 (59%)
 Frame = -3

Query: 155 KHSRNLWGPVDGLGAYTPPSLSNIHAL 75
           KHS +LWG  D L   TPP+  ++  L
Sbjct: 44  KHSADLWGSADALAIATPPASDDLRYL 70


>At3g61220.1 68416.m06851 short-chain dehydrogenase/reductase (SDR)
           family protein similar to carbonyl reductase GI:1049108
           from [Mus musculus]
          Length = 296

 Score = 30.3 bits (65), Expect = 1.2
 Identities = 16/48 (33%), Positives = 25/48 (52%), Gaps = 4/48 (8%)
 Frame = +2

Query: 491 QSQRFHQLDIA----TTKIMDFIKFESGNLCMITGGRNLGRVGTSCPA 622
           QS  FHQLD+A     T + +F+K + G L ++     +G + T   A
Sbjct: 59  QSLLFHQLDVADPASITSLAEFVKTQFGKLDILVNNAGIGGIITDAEA 106


>At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase,
           putative / steroid 5-alpha-reductase, putative identical
           to gi:1280611; contains a steroid 5-alpha reductase,
           C-terminal domain
          Length = 262

 Score = 29.1 bits (62), Expect = 2.8
 Identities = 15/39 (38%), Positives = 19/39 (48%)
 Frame = -3

Query: 245 TFMRRCFTIFRTSFPVKAYFRRFLRKITRGKHSRNLWGP 129
           TF R C      + P  A   +FL+    GKH+R  WGP
Sbjct: 8   TFFRYCLLTLIFAGPPTAVLLKFLQA-PYGKHNRTGWGP 45


>At5g61460.1 68418.m07712 structural maintenance of chromosomes
           (SMC) family protein very strong similarity to SMC-like
           protein (MIM) [Arabidopsis thaliana] GI:5880614;
           contains Pfam profile PF02463: RecF/RecN/SMC N terminal
           domain
          Length = 1057

 Score = 27.5 bits (58), Expect = 8.4
 Identities = 10/29 (34%), Positives = 16/29 (55%)
 Frame = +1

Query: 565 LVYDHGRP*LGACGHIVSRERHPGSFDIV 651
           ++YD  RP L    H+V +  HP  F ++
Sbjct: 532 IIYDFSRPRLNIPRHMVPQTEHPTIFSVI 560


>At5g03980.1 68418.m00378 GDSL-motif lipase/hydrolase family protein
           similar to lipase [Arabidopsis thaliana] GI:1145627;
           contains Pfam profile:PF00657 Lipase/Acylhydrolase with
           GDSL-like motif
          Length = 323

 Score = 27.5 bits (58), Expect = 8.4
 Identities = 13/28 (46%), Positives = 15/28 (53%)
 Frame = -3

Query: 278 RISRVSPNFPSTFMRRCFTIFRTSFPVK 195
           R   V+   P  F   CF I+ TSFPVK
Sbjct: 166 RAGAVNVVVPGNFPVGCFPIYLTSFPVK 193


>At2g38570.1 68415.m04738 expressed protein ; expression supported
           by MPSS
          Length = 302

 Score = 27.5 bits (58), Expect = 8.4
 Identities = 12/28 (42%), Positives = 17/28 (60%)
 Frame = +2

Query: 527 TKIMDFIKFESGNLCMITGGRNLGRVGT 610
           T I+ +IK   G   +  GGRN+G+V T
Sbjct: 196 TYILVYIKISVGYRVVTPGGRNIGKVST 223


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,655,619
Number of Sequences: 28952
Number of extensions: 335511
Number of successful extensions: 938
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 898
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 938
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1393347168
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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